간내 담석과 동반된 간내 담도암에서의 p53과 Ki-ras의 발현

김명환,이성구,서동완,이승규,남승우,박능화,민영일,김연석,심기남,공경엽 대한소화기학회 1997 대한소화기학회지 Vol.30 No.5

Background/Aims: Hepatolithiasis or primary intrahepatic stones may be accompanied later by intrahepatic cholangiocarcinoma. This cancerous lesion is frequently associated with atypical hyperplastic epithelium that was suspected of being precancerous. To investigate the Ki-ras or p53 mutation may play a role in carcinogenesis, and to determine whether atypical hyperplastic epithelium may be precancerous, this study was performed in intrahepatic cholangiocarcinomas associated with hepatolithiasis. Methods: We examined 12 patients with intrahepatic cholangiocarcinomas associated with hepatolithiasis. Ki-ras point mutations were assessed by direct DNA sequencing. Expressions of p53 protein were immunohistochemically assessed. The overexpression of p53 and point mutation of Ki-ras were examined in normal, hyperplastic, atypical hyperplastic, and can- cerous tissues separately. Results: The expressions of p53 protein were detected in 4 (33%) of 12 cholangiocarcinomas and Ki-ras point mutations at codon 12 were found in 2 (17%) of 12 cholangiocarcinomas. In those two carcinoma cases which contained the mutant sequence of Ki-ras, the same point mutation from wild type GGT (glycine) to GAT (aspartic acid) was. found in the associated atypical hyperplastic epithelium. However, none of the normal and hyperplastic epithelium harbored Ki-ras or p53 mutations. Conclusions: The overexpression of p53 may play a part in the carcinogenesis of some intrahepatic cholangiocarcinomas associated with hepatolithiasis, whereas the role of Ki-ras mutations in those cases is dubious. However, Ki-ras point mutation at codon 12 may be responsible for either cancer or atypical hyperplastic epithelium associated with hepatolithiasis in certain cases, suggesting atypical hyperplastic epithelium may give rise to carcinoma.

Tetrahydroisoquinoline Alkaloids가 쥐의 동맥과 뇌균등질의 α-Adrenoceptors에 미치는 영향

이현석,백선하,황수현,박인성,김은상,정진명,한종우,장기철,장기철 대한신경외과학회 1999 Journal of Korean neurosurgical society Vol.28 No.1

정신분열병에 대한 리스페리돈의 효과 및 안정성

이민수,김용구,김영훈,연병길,오병훈,윤도준,윤진상,이철,정희연,강병조,김광수,김동언,김명정,김상훈,김희철,나철,노승호,민경준,박기창,박두병,백기청,백인호,손봉기,손진욱,양병환,양창국,우행원,이정호,이종범,이홍식,임기영,전태연,정영조,정영철,정인과,정인원,지익성,채정호,한상익,한선호,한진희,서광윤 大韓神經精神醫學會 1998 신경정신의학 Vol.37 No.1

연구목적 : 본 시험의 목적은 임상시험 시작전에 연구자들을 대상으로 PANSS Workshop을 통하여 PANSS, ESRS에 대한 국내에서의 표준화 작업을 구축하고 새로운 정신병 치료제인 리스페리돈의 효과와 안정성을 재확인하여 리스페리돈 사용에 대한 적정화를 이루는데 있다. 연구방법 : 1996년 4월부터 1996년 9월까지 국내 39개 대학병원 정신과에 입원중인 혹은 증상이 악화되어 입원하는 정신분열병 환자 377명을 대상으로 다시설 개방 연구를 시행하였다. 1주일간의 약물 배설기간을 가진후, 리스페리돈을 8주간 투여하였고, 기준점, 1주, 2주, 4주, 그리고 8주후에 평가되었다. 용량은 제1일에는 리스페리돈 1mg씩 1일 2회, 제2일에는 2mg씩 1일 2회, 제3∼7일에는 3mg씩 1일 2회 투여하였다. 이후 환자의 임상상태에 따라 임의로 증량할 수 있으며, 최대 일일 16mg을 초과하지 않도록 하였다. 추체외로 증상을 조절하기 위한 투약을 허용하였다. 임상증상 및 부작용의 평가는 PANSS(Positive and Negative Syndrome Scale), CGI(Clinical Global Impression) 그리고 ESRS(Extrapyramidal Symptom Rating Scale)을 사용하였다. 연구결과 : 377명중 343명(91%)이 8주간의 연구를 완결하였다. 치료 종결시점인 8주후 PANSS 총점수가 20% 이상 호전된 경우를 약물 반응군으로 정의할때, 약물반응군은 81.3%였다. 리스페리돈에 반응하는 예측인자로는 발병연령, 이전의 입원 횟수, 유병기간이 관련 있었다. 리스페리돈은 1주후부터 PANSS양성, 음성, 및 일반정신병리 점수상에 유의한 호전을 보여 효과가 빨랐다. CGI의 경우도 기준점에 비해 1주후부터 유의한 감소를 나타내었다. ESRS의 경우, 파킨슨 평가점수는 기준점과 비교해 투여 1주, 2주, 4주후 유의하게 증가되었다가 8주후 기준점과 차이가 없었다. Dystonia 평가점수는 1주후만 유의한 증가를 보였으며, dyskinesia 평가점수는 유의한 차이가 없었다. 혈압, 맥박수의 생명징후 및 일반 혈액학 검사, 생화학적 검사, 심전도 검사에서 유의한 변화는 없었다. 결 론 : 이상의 다시설 개방 임상 연구를 통해 리스페리돈은 정신분열병 환자에서 양성증상뿐만 아니라 음성증상 및 전반적인 증상에도 효과적인 것으로 사료된다. 보다 명확한 평가를 위해서는 다른 항정신병약물과의 이중맹검 연구가 필요할 것으로 생각되며, 또한 장기적 치료에 대한 평가도 함께 이루어져야 하겠다. Objective : The purpose of this study was to investigate the efficacy and safety of risperidone in the treatment of Korean schizophrenic patients. Method : This multicenter open study included 377 schizophrenic patients drawn from 39 university hospitals. After a wash-out period of 1 week, the schizophrenic patients were treated with risperidone for 8 weeks and evaluated at 5 points ; at baseline, and 1, 2, 4 and 8 weeks of treatment. The dose was increased from 2mg/day(1mg twice daily) to 6mg/day(3mg twice daily) during the first week and adjusted to a maximum of 16mg/day over the next 7 weeks according to the patient's clinical response. Medication to control extrapyramidal symptoms was permitted. The psychiatric and neurological status of the patients was assessed by PANSS, CGI, and ESRS scales. Results : 343(91%) of 377 patients completed the 8-week trial period. Clinical improvement, as defined by a 20% or more reduction in total PANSS score at end point, was shown by 81.3% of patients. The predictors of response to risperidone were associated older age, shorter duration of illness, fewer previous hospitalization. Risperidone had rapid onset of action ; a significant decrease of the total PANSS and three PANSS factor(positive, negative, general), and CGI was already noticed at the end of first week. For the ESRS, parkinsonism rating scores were significantly increased until week 4 comparing with baseline. Dystonia rating scores were significantly increased until week 1, and dyskinesia rating scores were not significantly changed during the study. Laboratory parameters including vital sign, EKG, hematological, and biochemical values showed no significant changes during the trial. Conclusions : This study suggests that risperidone is generally safe and effective against both the positive and negative symptoms in our group of patients.

黃化水素化合物이 紫外線B를 照射한 黑色마우스의 表皮멜라닌細胞의 形態에 미치는 影響

明基範,鞠泓一 梨花女子大學校 韓國生活科學硏究院 1989 韓國生活科學硏究院 論叢 Vol.44 No.-

C57BL 흑색마우스에 UVB를 매일 100mJ/cm^2씩 10일간 조사하여 표피멜라닌세포를 활성화시킨 후 5% cysteine과 5% glutathione이 표피멜라닌세포의 수적 변화와 멜라닌소체 형태변화에 미치는 영향을 관찰하여 다음과 같은 결과를 얻었다. 1. Cysteine 투여군에서는 멜라닌세포의 수적변화가 없었으며 glutathione투여 5주 후에서만 멜라닌세포의 수가 감소하였다. 2. Cysteine 투여군과 glutathione 투여군은 대조군에 비해 표피멜라닌세포내 멜라닌소체의 단축 길이와 stage Ⅳ 멜라닌소체 비율에 유의한 감소를 보여 멜라닌형성 억제제로서 의의있는 효과를 나타내었다. 3. 두 실험군간의 비교에서 멜라닌소체 단축길이는 glutathione 투여군에서 투여 3주 후에 cysteine 투여군보다 의의있게 감소되었으며, stage Ⅳ 멜라닌소체의 비율은 glutathione 투여군에서는 투여 3주 후에 cysteine 투여군에서는 투여 5주 후에 각각 유의한 감소소견을 보였다. 4. Glutathione 투여군에서는 멜라닌소체 단축길이와 stage Ⅳ 멜라닌소체의 비율이 모두 투여기간에 비례하여 감소되었다. 이상과 같은 결과로 황화수소화합물인 cysteine과 glutathione은 자외선 B조사로 활성화된 표피멜라닌세포내 멜라닌소체의 단축길이와 stage Ⅳ 멜라닌소체의 비율을 감소시킴으로써 멜라닌형성을 억제하고, glutathione이 cysteine보다 멜라닌형성억제에 우수한 효과를 나타냄을 알수 있었다. The effects of sulfhydryl compounds, cysteine and glutathione, on populations of melanocytes, the sizes of melanosomes and the ratios of melanosomal stages of epidermal melanocytes in UVB-irradiated C57BL black mice were evaluated. The results were as follow: 1. Both of cysteine and glutathione showed no significant diminution in the numbers of dopa-positive melanocytes except at the end of 5 weeks' treatment with glutathione. 2. Both of cysteine and glutathione showed significant diminution in short axis of melanosomes and the percentage of stage Ⅳ melanosomes of epidermal melanocytes. 3. The lengthes of short axes of melanosomes in glutathione-treated group were smaller than those in cysteine-treated group at the end of 3rd week of intraperitoneal injection. 4. In the glutathione-treated group, the short axes of melanosomes and the percentages of stage Ⅳ melanosomes decreased in proportion to the period of intraperitoneal injection.

정신과 입원환자의 병원이탈에 대한 고찰

기문상,박상학,김명규 大韓神經精神醫學會 1996 신경정신의학 Vol.35 No.2

This study was a survey of all available hospital records pertaining to the 300 elopers of psychiatric inpatients admitted at Naju National Mental Hospital during the years between 1991-1993. The findings were compared with randomly selected control group of 300 inpatients who admitted during the same period. This study was designed to evaluate the demographic data and clinical characteristics, motivations, and results of elopement in these patients. Results were as follows : 1) During 3 years, 308 of 4670 inpatients(6.5%) committed elopememt. 2) Most of them were involuntarily admitted young male patients and they were likely to be unmarried, unemployed, and high-school graduated compared to control group. Their socioeconomic status were relatively low. 3) While the control group was mainly diagnosed as undifferentiated schizophrenia, more of elopers was diagnosed as paranoid schizophrenia. 4) Clinically, elopers had chronic course of illness, frequent and long-term hospitalization, and previous history of elopement than control group. 5) Patients in open wards showed more frequent elopement behavior than those in closed wards. They were likely to elope during the strolling. Many attempted to elope on Thursday when the family members visited them. 6) Most of the elopers were safely returned to the hospital and the management was simple interview without restraint. Many of families were not unconcerned about the patient's elopement. 7) Elopement group were characterized by their severe psychotic symptoms(hallucination, delusion). poor relationship with treatment teams, and cold familial concerns. It was suggested that the patient's intrapsychic factor(42.8%) was the most important factor in determining the elopement.

연령증가에 따른 흰쥐 삼차신경절 및 삼차신경감각핵에서의 Neuropeptide Y 면역반응세포의 분포에 관한 연구

김명국,백기석,이승표 대한해부학회 1996 Anatomy & Cell Biology Vol.29 No.6

場所性을 媒介體로 본 韓國伽藍建築空間의 中心이미지에 關한 硏究

예명해,최창길,이기승 대구대학교 산업기술연구소 1989 産業技術硏究 Vol.8 No.-

The following is the conclusion of survey and analysis of the main image of Korea's Buddhist temple space in view of placeness: In Korea, the stupa preserving relics of the Buddha enshrining the Buddhist bones at Tong Do Sa, the Buddhist-oriented temple, forms the main image of space. At Hae In Sa, the Truth-oriented temple, the Hall of Truth which enshrines the complete collection of Buddhist scriptutes, forms the main image of space. At Song Kwang Sa, the Bonze-oriented temple, the Hall of Sermon forms the main image of space. At Hwa Eum Sa. the general temple, the Main Buddha Sanctum enshrining the Buddha image forms the main image of space.

에틸렌디아민과 4배위형 에틸렌디아민-트리아세테이트를 포함하는 코발트(Ⅲ)착물에 관한 연구(제2보)

都命基,安昌祿 嶺南大學校附設 基礎科學硏究所 1982 基礎科學硏究 Vol.2 No.-

Octahedral cobalt(Ⅲ) complex containing tetradentate ethylenediamine-triacetate and ethylenediamine was prepared from the reaction of aqua(ethylenediamine-triacetato)cobalt(Ⅲ) with ethylenediamine in dimethylsulfoxide. Red-violet trans(0-0)(1) isomer is the [Co(en)(HEDTRA)]+ was isolated using a column (Φ5×60cm) of Dowex 50W-X8 cation exchange resin in H+ form. The UV, IR, and Circular Dichroism studies indicate that the red-violet complex has trans (0-0)(1) structure and the ethylenediamine-triacetate (EDTRA³-) ligand of [Co(en)(HEDTRA)]+ coordinates to cobalt(Ⅲ) ion as a tetradentate with a free acetate branch.

砲丸던지기 直前의 발位置에 對한 實驗的 硏究

白明基,崔大雨 충남대학교 자연과학연구소 1982 忠南科學硏究誌 Vol.9 No.1

For the experimental study of stance just before shot putting, we have trained 23 un-experienced and un-skilled students of department of physical education. College of Science, Chungnam University by O'Brein and Torque type of shot putting and made them put shots. The results was that the average record by the former type(45°stance) was 10m 39cm, and by the latter(90°stance) was 10m 99cm, and therefore the students with 90 stance threw farther than the ones with 45 stance by 60cm. Viewed from the above experiment, the shot putting of Torque type is considered to have better record than that of O'Brein type. Because the former type with much ywist of upper and lower body not only makes the throwing orbit longer, but pre-action of the Knee and waist unnecessary when they do shift-action, and there are no loss of power and no decrease of speed of lift. While our athletes use the type of 45°stance, only the foreign athletes with god physique and physical fitness get the good record by using the Torque of shot putting nowadays. Accordingly, we think that it is desirable for us change our present 45°type of stance into 90°type to get better record.

國際法上 北方政策과 北韓의 法的 地位에 관한 硏究

金明基 명지대학교 사회과학연구소 1990 사회과학논총 Vol.5 No.-