Slide Session : OS-IFD-07 ; Infectious Disease : In Vitro Antiviral Activity of Ribavirin Against Severe Fever with Thrombocytopenia Syndrome Virus

( Myung Jin Lee ),( Kye Hyung Kim ),( Jong Youn Yi ),( Su Jin Choi ),( Chung Jong Kim ),( Nak Hyun Kim ),( Kyoung Ho Song ),( Pyoeng Gyun Choi ),( Ji Hwan Bang ),( Wan Beom Park ),( Eu Suk Kim ),( San 대한내과학회 2014 대한내과학회 추계학술대회 Vol.2014 No.1

In Vitro Antiviral Activity of Ribavirin Against Severe Fever with Thrombocytopenia Syndrome Virus Myung Jin LEE1, Kye-Hyung KIM1, Jongyoun YI2, SuJin CHOI1, Chung-Jong KIM1, Nak- Hyun KIM1, Kyoung-Ho SONG1, Pyoeng Gyun CHOI1, Ji-Hwan BANG1, Wan Beom PARK1, Eu Suk KIM1, Sang-Won PARK1, Hong Bin KIM1, Nam Joong KIM1, Myoung- Don OH1 Seoul National University College of Medicine, Korea1, Pusan National University School of Medicine, Korea2 Background: Severe fever with thrombocytopenia syndrome (SFTS) is an emerging infectious disease caused by a novel Bunyavirus, severe fever with thrombocytopenia syndrome virus (SFTSV). No effective antiviral therapy is proven yet, but clinical use of ribavirin (RBV) has been tried. We investigated the antiviral effect of RBV against SFTSV in vitro. Methods: To test for cytotoxicity of RBV, Vero cells were treated with different concentrations of RBV (3.90 to 500 μg/mL, two-fold dilution) and analyzed by cell viability MTS assay 48h post-infection. To determine antiviral activity of RBV against SFTSV, Vero cells were infected with SFTSV strain Gangwon/Korea/2012 at 100 TCID50 (50% tissue culture infective dose) per well in a 96-well plate, and RBV was added at the concentrations showing no or minimal cytotoxicity. Viral RNAs were extracted from the culture supernatants and quantifi ed using one-step real-time reverse transcription- PCR to amplify the partial large segment of SFTSV. Statistical analysis was done by one-way ANOVA with Tukey`s post hoc test. Results: Cytotoxicity due to RBV was not observed at RBV concentration =31.3 μg/ mL. Viral RNAs at 24h post-RBV treatment were reduced with increasing RBV concentrations (1-32 μg/mL), compared with those of mock-treated cells (P <0.01, Figure). Half maximal inhibitory concentration (IC50) of RBV was 3.69 μg/mL at 24h post-RBV treatment. Conclusions: Our study shows that RBV has antiviral effect against SFTSV in a dose-dependent manner. Further studies are required to evaluate the effi cacy of RBV in SFTS.

토양환경지도 자료를 이용한 콩밭 토양의 화학성 비교

박지숙,이민진,정재원,김미혜,이서연,지윤미,한준호,김유학,공명석,한광현,노희명 忠北大學校 農業科學硏究所 2015 農業科學硏究 Vol.31 No.2

It is important to interpret the soil chemical properties in order to effectively manage the farmland. This study was conducted to investigate the soil chemical properties of upland soybean field according to the soil series at 32 upland fields located in the parts of Gangwon-do. Soil sampling sites were selected by using the National Statistics DB and KSIS DB that is a soil environment information system provided by the Rural Development Administration. Soil samples were collected from 0~15 cm of top soil before seeding or transplanting, air-dried, passed through a 2 mm sieve, and analyzed for soil pH, EC, organic matter, available phosphorus (P2O5), exchangeable potassium, exchangeable calcium and exchangeable magnesium. The average chemical properties have exceeded the each nutrient optimum range level of RDA, except the organic matter in experimental soil 2015. And despite application of conventional fertilization, each soil series nutrient contents were change respectively. The results of this study suggest that conventional fertilization should be applied based on the analysis about the amount of accumulated nutrient contents in the soil.

Gene pyramiding effect of two major bakanae disease resistance gene using qBK1 and qBKWD

Sais-Beul Lee,Tae-Heon Kim,Su-Min Jo,Jun-Hyun Cho,Ji-Yoon Lee,Jong-Hee Lee2,Dongjin Shin,You-Chun Song,Myung-Kyu Oh,Dong-Soo Park 한국작물학회 2017 한국작물학회 학술발표대회 논문집 Vol.2017 No.-

서울의 Penicillinase Producing Neisseria Gonorrhoeae 발생빈도(1996)

김재홍,황동규,전재홍,김윤석,김중환,김용준,이창균,임동진,김현수,조창근,김경문,박상훈,전우형,김희성,이호정,차명수,김갑형,김형석,김석우,황지환,박병순,권오상,이민수,송기훈,성소영,이인섭,부태성 대한화학요법학회 1999 대한화학요법학회지 Vol.17 No.2

Background : In recent years, gonorrhea has been panedemic and remains one of the most commom STDs in the world, especially in developing countries. Objective & Methods: For the detection of a more effective therapeutic regimen and assessing the prevalence of PPNG, we have been trying to study the patients who have visited the VD Clinic of Choong-Ku Public Health Center in Seoul since 1980 by means of the chromogenic cephalosporin method. Results: In 1996, 139 strains of N. gonorrhoeae were isolated, among which 53(39.0%) were PPNG. Conclusion: Our results suggests that after a peak of 74.3% in 1993, the prevalence of PPNG in Seoul is gradually declining.

노인의 영적 간호요구

최미혜,김경희,김귀옥,김기숙,김수강,김정신,김춘숙,노흥진,박지연,성혜연,오명선,이선희,이원옥,이윤영,이현수,장명재,차혜경,채정선,홍상희 중앙대학교 의과대학 간호학과 간호과학연구소 2001 중앙간호논문집 Vol.5 No.1

This study was designed to exam the aged's needs for spiritual nursing care. The purpose was to serve as a basis for the development of spiritual nursing practice. The major findings are as follows : 1. The degree of needs for spiritual nursing care as area was that needs of love and relationship mean 22.0, needs of meaning and object mean 28.2, needs of forgiving mean 13.5. Total needs for spiritual nursing care mean 63.7, which was on the upper middle level. The needs of meaning and object was rated highest. 2. Among the general characteristics of the subjects, needs of love and relatiohship wasn't significanlty different. 3. Among the general characteristics of the subjects, needs of meaning and object was significantly different according to two factors : age(F=7.260, p=0.001), religion(F=5.275, p=0.001). Higher needs of meaning and object was possessed by the older than the younger, by the one who have religion than the other. 4. Among the general characteristics of the subjects, four factors made a significantly difference to needs of forgiving : sex(t=-2.851, p=0.006), age(F=8.201, p=0.001), religion(F=6.928, p=0.000), disease(t=2.327, p=0.024). Higher needs of forgiving was possessed by man than woman, by the older than the younger, by the one who have religion than the other, by the one who have disease than the other.

경량 폴리머 콘크리트의 강도 및 난연성에 관한 연구

이윤수,주명기,김남길 강원대학교 석재복합신소재제품연구센터 2001 석재연 논문집 Vol.6 No.-

불포화 에스트테 수지가 가장 많이 이용되는 분야 중의 하나로 건축 내ㆍ외장재로 이용되고 있으나 여기서 지금까지 가장 문제로 되고 있는 것이 난연성 향상에 관한 것이다. 불포화 폴리에스터 수지는 탄소를 구성 성분으로 하는 유기물이므로 무기재료와 같은 불연성을 기대하기는 어려우나 지금까지의 연구결과 불연성 첨가제 등을 이용하여 어느 정도까지는 연소를 억제시킬 수는 있다는 것이다. 본 연구에서는 폴리머 콘크리트의 난연성 성능을 판단하기 위해 난연제가 이미 포함되어 시판되는 불포화 에스트테 수지를 결합재로 사용하고 탄산칼슘을 충진재로, 규사를 잔골재로 치환하고, 굵은골재를 경량골재로하는 경량 폴리머 콘크리트를 제조하여 결합재 첨가량과 규사 치환율을 변화시켜 변수별 난연특성을 구명하였던 바, 최적의 결합재 첨가량과 규사 치환율을 얻을 수 있었다. In this study, the light weight polymer concrete were produced, in which unsaturated polyester resin was used as a binder, calcium carbonate as a filler, silica sand and perlite as a fine aggregate, and expanded shale as a coarse aggregate. The effect of binder content and silica sand content that influence on strength and incombustibility are examined. Results show that the compressive, flexural and tensile strengths of the lightweight polymer concretes increase with increasing binder content as well as silica sand content. Addition of the unsaturated polyester resin with the flame retarder is effective for improving the incombustible properties of lightweight polymer concrete.

Jini 기반 전자상거래 구조의 설계

이병래,김지균,이명선 高麗大學校附設 컴퓨터科學技術硏究所 1999 JOURNAL OF COMPUTER SCIENCE & ENGINEERING TECHNOLO Vol.1 No.-

시간이 지날수록 다양한 장소는 네트워크화 되고 있으며, 많은 종류의 전자 기계와 소프트웨어 또한 네트워크화 되고 있다. 이러한 조건하에서, 분산 환경은 필수적인 요건이 될 것이고, 전자 기계 또는 소프트웨어들의 호환성은 고려되어야 할 것이다. 이 논문에서는 Jini를 기반으로 한 전자상거래 구조를 제시한다. Jini 시스템은 Java 중심적인 분산 시스템으로서 단순성, 유연성 그리고 연합성을 특징으로 하고 있다. Jini 구조는 머신 또는 프로그램들이 연합체에 참가하여 다른 멤버들에게 리소스를 제공하고 필요한 리소스를 사용하는 기반을 제공한다. Jini 구조는 Java 언어 코드의 이동성을 취하여 service라는 개념 하에, Jini 시스템의 사용자로부터 이용되는 하드웨어에서의 소프트웨어 또는 하드웨어 자신의 컴포넌트들까지 통합한다. In the future, Countless place should be networked and Countless variety of money-related electronic requirement, and electronic machine's interoperability should be concerned. In this paper, we propose Jini based electronic commerce architecture. A Jini system is a Javatm-centric distributed system designed for simplicity, flexibility, and federation. The Jini architecture provides mechanisms for machines or programs to enter into a federation where each machine or program offers resources to other members of the federation and uses resources as needed. The design of the Jini architecture exploits the ability to move Java language code from machine to machine and unifies under the notion of a service everything from the user of a Jini system to the software available on the machines to the hardware components of the machines themselves.

감식초 및 감고추장을 이용한 감초고추장의 최적 배합비 설정

이기동,정용진,서지형,이명희 동아시아식생활학회 1998 동아시아식생활학회지 Vol.8 No.3

The optimum recipe of persimmon chokochujang using persimmon vinegar and kochujang was established by four-dimensional response surface methodology. The organoleptic color of persimmon chokochujang showed maximum score in 18.16g of persimmon vinegar, 5.58g of garlic and 1.62g of sucrose. The organoleptic aroma of persimmon chokochujang showed maximum score in 18.19g of persimmon vinegar, 4.57g of garlic and 2.06g of sucrose. The organoleptic taste of persimmon chokochujang showed maximum score in 19.28 g of persimmon vinegar, 4.97g of garlic and 2.34g of sucrose. The organoleptic color of persimmon chokochujang showed maximum score in 18.81 g of persimmon vinegar, 4.81g of garlic and 2.65g of sucrose. Optimum mixing ranges for organoleptic properties of persimmon chokochujang were 18.25~19.25g of persimmon vinegar, 4.60~5.00g of garlic and 2.06~2.65g of sucrose.

레이저 포화흡수체 Nd:LSB에 관한 연구

지명훈,오세용,이영우 목원대학교 멀티미디어신기술연구소 2001 멀티미디어신기술연구소논문집 Vol.1 No.1

본 논문에서는 다른 Q-스위칭용 포화흡수체보다 넓은 천이 영역을 가지고 있는 포화흡수체 Nd:LSB가 Q-스위칭 소자로서 가능한지 보였으며, 최적화된 출력 거울의 반사율, 최대 출력, 출력 에너지, 펄스폭, 출력 효율을 수치적으로 분석하였다. 그 결과 Nd:LSB를 이용한 1319nm 파장의 수동형 Q-스위칭 Nd:YAG 레이저의 특성을 예측할 수 있었고, 공진기 거울의 반사율 98%, 공진기 길이 30㎝로 하였을 경우 출력 31.3MW, 펄스폭 6.22ps를 얻을 수 있다. In this study, it's shown that the saturable absorber Nd:LSB which has broad absorption bandwidth than other saturable absorber as Q-switching system has a capability as a saturable absorber in Q-switching system. In this system, we theoretically analyzed the optimized output mirror, peak power, output power, pulse width and output efficiency. As the result, the passively Q-switched 1319nm Nd:YAG laser using Nd:LSB as a saturable absorber gives the optimized output mirror reflectivity 98% and peak power of 31.3MW and pulse width of 6.22ps at 30㎝ resonator length theoretically.

Postweaning Multisystemic Wasting Syndrome (PMWS)에 이환된 자돈에 대해 Porcine circovirus 2 Rep 유전자 검출에 관한 연구

이용훈,김지영,楚佳奇,서상희,장경수,김명철,박창식,전무형 충남대학교 수의과대학 동물의과학연구소 2004 動物醫科學硏究誌 Vol.12 No.-

Postweaning multisystemic wasting syndrome (PMWS) is a new emerging disease in swine herds world wide, and porcine circovirus type 2 (PCV2) is known as a major cause of PMWS. In this study, the prevalence of PCV2 Rep gene was examined for the serum, nasal swabs and feces collected from 30 heads of piglets affected with PMWS and 10 heads of normal healthy piglets using polymerase chain reaction (PCR). The diagnostic efficiency of the PCR was evaluated for different types of samples. Rep gene of the isolate (PCV2 S1) was then sequenced. In the group of PMWS-affected piglets, the prevalence of PCR-positive were 70.0% in sera, 33.3% in nasal swabs and 0% in feces. In the normal healthy group, the PCR-positive rates of sera, nasal swabs and feces were 30%, 20% and 0%, respectively. Seven piglets (17.5%) out of 40 showed the simultaneously positive results in both of sera and nasal swabs. The nucleotide sequence homology of Rep gene of PCV2 S1 as compared with the reference strain of PCV2 (GenBank accesson no.AF027217) was 99.3%.