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Vinblastine이 백서 배양 Type-1 별아교세포에 미치는 세포독성에 관한 연구
하상호,박승택,문연자,김종영,김정중,정연태 圓光大學校 醫科學硏究所 1994 圓光醫科學 Vol.10 No.1-2
It has been reported that vinblastine, anticancer drug, is a neuroteratogen inducing various neural malformations such as microcephaly and neural tube defect(NTD), but the study on the toxicity of neuroglial cells, especially type-1 astrocytes using culture system is not well established. In order to evaluate the cytotoxicity of vinblastine on cultured type-1 astrocytes of neonatal rat brain in vitro. MTT assay and also light and electron microscopic studies were carried out. The results were as follows: 1. MTT_90 and MTT_50 in cultured type-1 astrocytes were 1×10 exp (-1) μM and 1×10 exp (2)μM of vinblastine, respectively. 2. Vinblastine was highly toxic in cultured type-1 astrocytes(MTT_50 ≤ 100 μM). 3. In a light microscopy, cultured type-1 astrocytes showed decrease of cells in number, cytoplasmic perforation, vacuolization and cytoplasmic granulation after cultured type-1 astrocytes were treated with 1×10 exp (2)μM of vinblastine for 24 hours. Cultured type-1 astrocytes damaged by vinblastine showed degenerative changes of cells morphologically. 4. In an electron microscopy, increment of glial fibrillary acidic protein(GFAP), increased free-ribosomes, cisternal dilatation of rough endoplasmic reticulum (rER). few dense bodies and vacuoles were shown in cultured type-1 astrocytes treated with 1×10 exp (2)μM of vinblastine for 24 hours. The results indicate that vinblastine has markedly cytotoxic effect on the type-1 astrocytes of neonatal rat brain in vitro study.
진세노사이드 Rd와 사포닌 대사물인 compound K의 항지질과산화 효과
김경현,성금수,문연자,박시준,신미란,장재철 한국전통의학연구소 2006 한국전통의학지 Vol.15 No.1
To study on antioxidant effects in the liver of 40-week-old mouse, the sample were orally pretreated 5mg/kg/day for 5 days with red ginseng saponin components(total saponin, protopanaxadiol saponin, protopanaxatriol saponin, ginsenoside-Rd, ginsenoside-Re, compound-K) for 5 days. The ability of saponin to protect the mouse liver from oxidative damage was examined by determining the activity of superoxide dismutase(SOD), glutathione peroxidase(GPx) and the contents of glutathione, the level of malondialdehyde. The only protopanaxadiol among the ginseng saponin fractions was significantly increased the hepatic SOD activity(p<0.01). The red ginseng saponin induced a slight increase of GPx activity, especially ginsenoside Rd, compound K and protopanaxatriol treatments significantly increased its activity. The content of glutathione was significantly increased by total saponin, protopanaxadiol and ginsenoside Rd(p<0.01), but the oxidized glutathione level was lowered in all the red ginseng saponin. Finally, the level of malondialdehyde was significantly decreased by ginsenoside Rd and protopanaxadiol. In conclusion, protopanaxadiol and ginsenoside Rd among the saponin fraction were especially increased in the activity of hepatic antioxidative enzyme and decreased the lipid peroxidation that was expressed in term of MDA formation. This comprehensive antioxidant effects of red ginseng saponin seems to be by a certain action of saponin other than a direct antioxidant action.
생쥐의 卵子와 初期胚子에 對한 Clomiphene Citrate의 細胞毒性에 關한 硏究
金惠敬,潘勝一,金洋一,文蓮子,朴承澤,鄭然泰 圓光大學校 醫科學硏究所 1993 圓光醫科學 Vol.9 No.1-2
In order to elucidate the cytotoxicity of clomiphene citrate, the rate of in vitro fertilization(IVF) of ova and the developmental rate of early embroys in A-strain mouse were examined. The rate of ova cleavaging to 2-cell stage by IVF was remarkably decreased(12.6%) at the concentration of 10μg/㎖ of clomiphene than that of the control(65.2%) when the ova fertilized in vitro were cultured for 24 hours in the untreated medium after treatment ova with clomiphene for 5 hrs. But there was no ovum cleavaging to 2-cell stage at 50μg/㎖ clomiphene except only a few 2-polar bodied ova. The developmental rate of mouse early embryos was decreased dose-dependently. Especially at 25μg/㎖ of clomiphene, the number of embryos cleavaging to blastocyst from 2-cell stage(41.4%) was decreased remarkably compared with that of the control(87.2%). And only a few embryos were developed to 8-cell stage at clomiphene concentration of 50μg/㎖ after 2-cell embryos were incubated for 72 hours in clomiphene-treated medium. Morphological changes such as fragmentation and fusion of blastomeres, cytolysis and developmental retardation were increased with dose-dependently. These results suggest that clomiphene has cytotoxic effect by the decrease of the rate of IVF, the developmental rate and the degenerative changes of murine ova and early embryos.
박시준,김호민,한규수,성금수,신미란,문연자,우원홍 한국전통의학연구소 2006 한국전통의학지 Vol.15 No.1
Lavandula vera is indispensable member of the herb family, used for perfumes and potpourri. Androgens have profound effects on the physiology of the sebaceous gland. Sebum is secreted due to the effect of androgen, which starts to be secreted at puberty. Using the human sebocyte cell line SZ95, the author investigated the inhibitory effect of Lavandula vera on the lipid production. Light microscopic finding were examined numerous cytoplasmic lipid droplets SZ95 cells by Oil red staining and lipid droplets were increased markedly by testosterone. On the other hand, combined treatment with Lavandula vera and testosterone resulted in a lower lipid droplets than with testosterone alone in a dose-dependent manner. These findings indicate that Lavandula vera acts antagonistically to testosterone and inhibits the lipid synthesis of SZ95 cells at the cellular level.
남항우,이성원,이병주,권칠용,문연자,안성훈,우원홍 한국전통의학연구소 2002 한국전통의학지 Vol.12 No.1
The Sellaginella Tarmariscina is widely used in the traditional oriental herbal medicine for its anti-inflammatory, antioxidant effects. The effects of aqueous extracts of Sellaginella Tarmariscina on the viability and induction of apoptotic cell death were investigated in HL-60 cells. The cell viability after treating with extract of Sellaginella Tarmariscina was quantified by MTT assay. The results showed that could inhibit the proliferation of HL-60 cells and caused a 40% inhibition of HL-60 cells at concentrations of 400 ㎍/ml. The cancericidic effect of Sellaginella Tarmariscina was mediated by apoptosis. Thus, HL-60 cells exposed to Sellaginella Tarmariscina displayed the DNA fragmentation ladder and nucleus chromatin condensation characteristic for apoptosis. In conclusion, our results suggest that the extract of Sellaginella Tarmariscina may induce the apoptotic death in HL-60 cells.
김대성,노성택,이장천,임규상,신미란,우원홍,문연자 한국전통의학연구소 2006 한국전통의학지 Vol.15 No.1
The aim of this study was to investigate the effect of ethanol extract of Fagopyrum escuentum(FE) on the melanogenesis. To determine whether ethanol extract of FE suppress melanin synthesis in cellular level, B16F10 melanoma cells were cultured in the presence of different concentrations of FE ethanol extract. In the present study, the author examined the effects of FE ethanol extract on cell proliferation, melanin contents, tyrosinase activity. Cell proliferation was slightly increased by treatment with ethanol extract of FE (25-200 ㎍/ml). The ethanol extract of FE effectively suppressed melanin contents at a dose of 100 ㎍/ml. It was observed that the color of cell pellets was totally whitened compared with the control. The ethanol extract of FE inhibited tyrosinase activity, regulate melanin biosynthesis as the key enzyme in melanogenesis. These results suggest that the ethanol extract of FE exerts its depigmenting effects through the suppression of tyrosinase activity. And it may be a potent depigmetation agent in hyperpigmentation condition.