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Fluorescent Bead–DNA Conjugate-based Dual Signal Amplification Technology
Mukesh Digambar Sonawane,Hae Ryong Lee,Satish Balasaheb Nimse,Keum-Soo Song,김태선,김상철 대한화학회 2016 Bulletin of the Korean Chemical Society Vol.37 No.5
One of the major challenges in DNA microarrays for their widespread application is assay sensitivity. Therefore, to achieve higher sensitivity, we developed a dual signal amplification technology based on the fluorescence beads (FB). It is the first report of the development of a dual signal amplification technology for DNA microarrays by synthesizing and applying the FB–DNA conjugates. The FB–DNA conjugates and Cy5-T1-FB-mixed-DNA demonstrated ~250–325-fold increase in sensitivity when compared with the conventional use of Cy5-labeled oligonucleotides such as Cy5-T1. Furthermore, the Cy5-T1-FB-mixed-DNA demonstrated ~5–1.3-fold increase in the sensitivity when compared with that of the FB–DNA conjugates alone.
Biomarker detection technologies and future directions
Nimse, Satish Balasaheb,Sonawane, Mukesh Digambar,Song, Keum-Soo,Kim, Taisun The Royal Society of Chemistry 2016 The Analyst Vol.141 No.3
<P>Biomarkers play a vital role in disease detection and treatment follow-up. It is important to note that diseases in the early stage are typically treated with the greatest probability of success. However, due to various technical difficulties in current technologies for the detection of biomarkers, the potential of biomarkers is not explored completely. Therefore, the developments of technologies, which can enable the accurate detection of prostate cancer at an early stage with simple, experimental protocols are highly inevitable. This critical review evaluates the current methods and technologies used in the detection of biomarkers. The aim of this article is to provide a comprehensive review covering the advantages and disadvantages of the biomarker detection methods. Future directions for the development of technologies to achieve highly selective and sensitive detection of biomarkers for point-of-care applications are also commented on.</P>
A glass fibre membrane platform for ultra-sensitive detection of cardiac troponin T
Song, Keum-Soo,Nimse, Satish Balasaheb,Sonawane, Mukesh Digambar,Lin, Yahui,Zhou,Kim, Taisun The Royal Society of Chemistry 2017 The Analyst Vol.142 No.20
<P>A glass fibre membrane platform that allows quantification of circulating cTnT with a LoD of 0.87 pg mL<SUP>−1</SUP> is described. The proposed platform uses a glass fibre membrane, DNA-guided detection method, and antibody-conjugated fluorescent beads for the quantification of cTnT in the analytical detection range of 1-120 pg mL<SUP>−1</SUP> at room temperature in 30 min. Glass fibre membranes were chemically modified to immobilize the oligonucleotide probes that catch a biomolecular complex (FB-dAB-cTnT-cAB-DNA) containing complementary oligonucleotides. There were no interferences from human cTnI, cTnC, skTnT, biotin, and hemoglobin (each 1 μg mL<SUP>−1</SUP>). The linearity in the serial dilution test of plasma samples indicates that this platform is highly applicable for regular health check-up to assess the risk of AMI and HF.</P>