http://chineseinput.net/에서 pinyin(병음)방식으로 중국어를 변환할 수 있습니다.
변환된 중국어를 복사하여 사용하시면 됩니다.
Haque, Muhammad R.,Jeong, Jee-Heon,Byun, Youngro Elsevier 2016 Biomaterials Vol.84 No.-
<P><B>Abstract</B></P> <P>This study suggests a novel method of stabilizing fragile porcine islets to prevent the dissociation after isolation and reducing immune cell invasion in a combination therapy of ‘surface camouflaging’ and immunosuppressive drugs (FK506, Rapamycin, MR-1, anti-CD19 mAb, and Clodrosome<SUP>®</SUP>) to effectively alleviate overall immune reactions against xenotransplanted porcine islets. The surface camouflage of pancreatic islets using biocompatible materials improved stabilization of pancreatic islet and prevented the infiltration of immune cells. Firstly, the surface of porcine islets was camouflaged by SH-6-arm-PEG-lipid and gelatin-catechol (artificial extracellular matrix) in order to stabilize the fragile isolated islets. Secondly, three different PEG layers (6-arm-PEG-SH, 6-arm-PEG-catechol, and linear PEG-SH) were chemically conjugated onto the surface of the stabilized porcine islets. Both artificial extracellular matrix (artificial ECM) and PEGylation effectively covered the surface of porcine islets without increasing the size of the whole islet. In addition, the viability and functionality of the islets were not affected by this multi-layer surface modification. The multi-layer modification significantly reduced the attachment of human serum albumin, fibronectin, and immunoglobulin G in comparison to the control collagen surface. The combination effect of multi-layer PEGylation and cocktailed immunosuppressive drugs on the survival time of the transplanted islets was assessed in a xenogeneic porcine-to-mouse model. The median survival time (MST) of ‘artificial ECM + PEGylation' group was 4-fold increased compared to that of control group. In addition, the MST of ‘artificial ECM + PEGylation + drug' group was 2.16-fold increased, compared to the ‘control + drug’ group. In conclusion, we proposed a novel porcine islet transplantation protocol using surface multi-layer modification and cocktailed immunosuppressive drugs, for stabilization and immunoprotection against xenogeneic immune reactions.</P>
( Muhammad R. Haque ),( Dong Yun Lee ),( Cheol Hee Ahn ),( Jee Heon Jeong ),( Young Ro Byun ) 영남대학교 약품개발연구소 2015 영남대학교 약품개발연구소 연구업적집 Vol.25 No.-
Purpose The purpose of this study was to investigate the effect of locally delivered pancreatic islet with liposomal clodronate (Clodrosome®) as an immunoprotection agent for the treatment of type 1 diabetes. Method The bio-distribution of liposomal clodronate in matrigel was checked by imaging analyzer. To verify the therapeutic efficacy of locally delivered islet with liposomal clodronate using injectable hydrogel, four groups of islet transplanted mice (n=6 in each group) were prepared: 1) the islet group, 2) the islet-Clodrosome group, 3) the islet-Matrigel group, and 4) the islet-Matrigel- Clodrosome group. Immune cell migration and activation, and pro-inflammatory cytokine secretion was evaluated by immunohistochemistry staining and ELISA assay. Results Cy5.5 labeled liposomes remained in the matrigel for over 7 days. The median survival time of transplanted islets (Islet- Matrigel-Clodrosome group) was significantly increased (>60 days), compared to other groups. Locally delivered liposomal clodronate in matrigel effectively inhibited the activation of macrophages, immune cell migration and activation, and proinflammatory cytokine secretion from macrophages. Conclusions Locally co-delivered pancreatic islets and liposomal clodronate using injectable hydrogel effectively cured type 1 diabetes. Especially, the inhibition of macrophage attack in the early stage after local delivery of islets was very important for the successful long-term survival of delivered islets.
( Muhammad R Haque ),( Jee Heon Jeong ),( Youngro Byun ) 영남대학교 약품개발연구소 2016 영남대학교 약품개발연구소 연구업적집 Vol.26 No.-
This study suggests a novel method of stabilizing fragile porcine islets to prevent the dissociation after isolation and reducing immune cell invasion in a combination therapy of ``surface camouflaging`` and immunosuppressive drugs (FKS06, Rapamycin, MR-l, anti-CDl9 mAb, and Clodrosome") to effectively alleviate overall immune reactions against xenotransplanted porcine islets. The surface camouflage of pancreatic islets using biocompatible materials improved stabilization of pancreatic islet and prevented the infiltration of immune cells. Firstly, the surface of porcine islets was camouflaged by SH-6-arm-PEG-lipid and gelatin-catechol (artificial extracellular matrix) in order to stabilize the fragile isolated islets. Secondly, three different PEG layers (6-arm-PEG-SH, 6-arm-PEG-catechol, and linear PEG-SH) were chemically conjugated onto the surface of the stabilized porcine islets. Both artificial extracellular matrix (artificial ECM) and PEGylation effectively covered the surface of porcine islets without increasing the size of the whole islet. In addition, the viability and functionality of the islets were not affected by this multi-layer surface modification. The multi-layer modification significantly reduced the attachment of human serum albumin, fibronectin, and immunoglobulin G in comparison to the control collagen sur-face, The combination effect of multi-layer PEGylation and cocktailed immunosuppressive drugs on the survival time of the transplanted islets was assessed in a xenogeneic porcine-to-mouse model. The median survival time (MST) of ``artificial ECM + PEGylation`` group was 4-fold increased compared to that of control group. In addition, the MST of ``artificial ECM + PEGylation + drug`` group was 2.16-fold increased, compared to the ``control + drug`` group. In conclusion. we proposed a novel porcine islet transplantation protocol using surface multi-layer modification and cocktailed immunosuppressive drugs, for stabilization and immunoprotection against xenogeneic immune reactions.
Polymeric nano-shielded islets with heparin-polyethylene glycol in a non-human primate model
Park, Hyojun,Haque, Muhammad R.,Park, Jae Berm,Lee, Kyo Won,Lee, Sanghoon,Kwon, Yeongbeen,Lee, Han Sin,Kim, Geun-Soo,Shin, Du Yeon,Jin, Sang-Man,Kim, Jae Hyeon,Kang, Hee Jung,Byun, Youngro,Kim, Sung J Elsevier 2018 Biomaterials Vol.171 No.-
<P><B>Abstract</B></P> <P>Intraportal pancreatic islet transplantation incurs huge cell losses during its early stages due to instant blood-mediated inflammatory reactions (IBMIRs), which may also drive regulation of the adaptive immune system. Therefore, a method that evades IBMIR will improve clinical islet transplantation. We used a layer-by-layer approach to shield non-human primate (NHP) islets with polyethylene glycol (nano-shielded islets, NSIs) and polyethylene glycol plus heparin (heparin nano-shielded islets; HNSIs). Islets ranging from 10,000 to 20,000 IEQ/kg body weight were transplanted into 19 cynomolgus monkeys (n = 4, control; n = 5, NSI; and n = 10, HNSI). The mean C-peptide positive graft survival times were 68.5, 64 and 108 days for the control, NSI and HNSI groups, respectively (<I>P</I> = 0.012). HNSI also reduced the factors responsible for IBMIR <I>in vitro</I>. Based on these data, HNSIs in conjunction with clinically established immunosuppressive drug regimens will result in superior outcomes compared to those achieved with the current protocol for clinical islet transplantation.</P>
정지헌,우윤주,이미현,Muhammad R. Haque,안철희,이해신,변영로 한국공업화학회 2018 Journal of Industrial and Engineering Chemistry Vol.57 No.-
Porcine islets are an alternative cell source for cell replacement therapy in diabetes. However, porcineislets are very fragile, and isolated islets are easily damaged during culture. Therefore, the purpose of thisstudy was to incorporate a tissue-adhesive chitosan derivative on the surface of porcine islets to preventthe dissociation of porcine islet clusters into single cells. Chitosan-catechol was evenly covered on thesurface of islets without any toxicity. In addition, the tissue-adhesive chitosan catechol was effective inpreventing cell dissociation. Therefore, tissue adhesive chitosan-catechol conjugation onto the porcineislet surface is a promising technique to stabilize islets after isolation.