Simple Sequence Repeat (SSR) and GC Distribution in the Arabidopsis thaliana Genome

Mortimer Jennifer C,Batley Jacqueline,Love Christopher G,Logan Erica,Edwards David The Korean Society of Plant Biotechnology 2005 Plant molecular biology and biotechnology research Vol.7 No.1

We have mined each of the five A. thaliana chromosomes for the presence of simple sequence repeats (SSRs) and developed custom perl scripts to examine their distribution and abundance in relation to genomic position, local G/C content and location within and around transcribed sequences. The distribution of repeats and G/C content with respect to genomic regions (exons, UTRs, introns, intergenic regions and proximity to expressed genes) are shown. SSRs show a non-random distribution across the genome and a strong association within and around transcribed sequences, while G/C density is associated specifically with the coding portions of transcribed sequences. SSR motif repeat number shows a high degree of variation for each SSR type and a high degree of motif sequence bias reflecting local genome sequence composition. PCR primers suitable for the amplification of identified SSRs have been designed where possible, and are available for further studies.

Impact of Intravenous Aspirin Administration on Ventriculostomy-Associated Hemorrhage in Coiled Acute Subarachnoid Hemorrhage Patients

Alex Mortimer,David Evans,Richard Flood,Owain Davies,James Wareham 대한신경중재치료의학회 2021 Neurointervention Vol.16 No.2

Purpose Aspirin has beneficial effects on coiling, even in acute subarachnoid hemorrhage, but there is also a perceived risk of increased bleeding and, importantly, a concern regarding ventriculostomy-associated hemorrhage (VAH) in those with complicating hydrocephalus. We aimed to assess the rate and extent of VAH in patients specifically treated with procedural intravenous aspirin during endovascular coiling of ruptured intracranial aneurysms. Materials and Methods This was a single neurovascular center retrospective observational study of consecutive patients treated over a three-year period. The rate of VAH assessed using computed tomography and clinical outcomes were compared in patients receiving intraprocedural intravenous aspirin loading (n=90) versus those that did not receive the drug (n=40). Results There was a significantly elevated rate of VAH in patients receiving intravenous aspirin (30% vs. 2.5%, odds ratio 16.7 [95% confidence interval: 2.2–128.0], P<0.0001). The majority of VAH was <10 mm in size (70%) with the largest bleed measuring 20 mm. No hematoma required surgical evacuation. No difference in favorable outcome at discharge was demonstrated. There was no difference in mortality between the 2 groups. Conclusion Loading with intravenous aspirin during endovascular treatment of ruptured intracranial aneurysms significantly increases the risk of VAH, but most are small with minimal impact on clinical outcome at discharge. Intravenous aspirin should probably be reserved for selected cases but should not be withheld based on risk of VAH.

EFFECT OF FEEDING PATTERN ON DIURNAL VARIATION IN FAECAL CHROMIC LEVEL WHEN USING CONTROLLED RELEASE DEVICES IN SHEEP

Lee, G.J.,Mortimer, S.I. Asian Australasian Association of Animal Productio 1991 Animal Bioscience Vol.4 No.1

Diurnal variation in faecal chromic oxide levels was estimated from 4 hourly faecal sampling of 56 wethers allotted to one of six different feeding patterns. Sampling occurred on days 6 and 7 (Period 1) after a controlled release device was administered, and was repeated on days 14 and 15 (Period 2) following re-randomisation of the wethers to the feeding patterns. Increasing the frequency of feeding tended to be associated with higher faecal chromic oxide levels (p < 0.05), particularly in wethers fed thrice daily at 8 hour intervals. There was no interaction between feeding pattern and period, sampling day or time within-day. There were significant period x time within-day (p < 0.05) and day x time within-day (p < 0.001) interactions, indicating that variation in faecal chromic oxide between sample times was not consistent. This implies that sampling at any time of day is unlikely to result in a biased estimate of pasture intake, providing sufficient samples are collected. Significant period (p < 0.001) and period x day (p < 0.01) effects were associated with slow faecal chromic oxide equilibration in period 1. Equilibration did not occur until after day 7, indicating a need for caution when commencing sampling.

Resveratrol protects SH-SY5Y neuroblastoma cells fromapoptosis induced by dopamine

Mi Kyung Lee,Soon Ja Kang,Mortimer Poncz,송기준,박광숙 생화학분자생물학회 2007 Experimental and molecular medicine Vol.39 No.3

Dopamine (DA) is an oxidant that may contribute to the degeneration of dopaminergic neurons. The present study demonstrates that DA-induced cytotoxicity in human-derived neurotypic cells, SH-SY5Y, is prevented by resveratrol, one of the major antioxidative constituents found in the skin of grapes. SH-SY5Y cells, a neuroblastoma cell line, treated with DA at 300 and 500 M for 24 h underwent apoptotic death as determined by characteristic morphological features, including nuclear conden-sation, and loss of mitochondrial membrane poten-tial (MMP). Flow cytometric analysis using Annexin V showed that DA can induce significant and severe apoptosis. Exposure to resveratrol (5 M) for 1 h prior to the DA treatment attenuated DA-induced cyto-toxicity, and rescued the loss of MMP. To investigate the apoptotic signaling pathways relevant to the restoration of DA-induced apoptosis by resveratrol, we carried out quantitative analysis of Bcl-2, cas-pase-3, and cleaved poly ADP-ribose polymerase (PARP) by immunoblot analysis. Resveratrol pre-treatment led to a decrease in cleavage of PARP, an increase in the Bcl-2 protein, and activation of cas-pase-3. These results suggest that DA may be a potential oxidant of neuronal cells at biologically relevant concentrations. Resveratrol may protect SH-SY5Y cells against this cytotoxicity, reducing intracellular oxidative stress through canonical signal pathways of apoptosis and may be of bio-logical importance in the prevention of a dopami-nergic neurodegenerative disorder such as Parkin-son disease.

In vivo characterization of the integrin b3 as a receptor for Hantaan virus cellular entry

Jin-Won Song,Ki-Joon Song,Luck-Ju Baek,Blasie Frost,Mortimer Poncz,KwangSook Park 생화학분자생물학회 2005 Experimental and molecular medicine Vol.37 No.2

Binding of viruses to cel surface molecules is an essential step in viral infection. In vitro studies suggested that the αvβ3 integrin receptor is the epithelial cell receptor for Hantan virus (HTNV). Whether β3 is in vivo the only or central cellular receptor for HTNV infection is not known. To investigate the role of β3 integrin for cellular model in newborn murine pups. Infected pups died at an average age of 14.21.1 days with high levels of viral antigen detected in their brain, lung, and kidney. Pre-injection of blocking mono-clonal antibodies (mAb) specific for either β3 or αv prolonged survival significantly to a maximal average survival of 19.7 1.5 days (P < 0.01) and 18.4 0.9 days (P < 0.01), respectively. XT- 19, a chemical blocker of the αvβ3 receptor also prolonged survival to 19.5 1.3 days (P < 0.01). In contrast to these receptor blockades, anti- HTNV antibody was not only able to prolong survival, but 20% of infected pups achieved long- term survival. An anti-murine β1 antibody compa-ratively prolonged survival (19.0 1.2 days), sugesting that HTNV infection is partly mediated through integrin β1 receptors as wel as through β3 receptors in vivo. Our data demonstrate that the β3 receptor is important for HTNV infection in vivoaditional receptors beyond β3 for celular entry within an organism.

제1부 잡초 분야 : 토양조건 및 파종심도에 따른 벼 및 잡초의 출아율

구연충(Yeon Chung Ku),허일봉(I . P . Huh),(Martin Mortimer) 한국잡초학회 1997 한국잡초학회 별책(학술대회 초록집) Vol.17 No.1

Weed Emergence as Affected by Burying Depth and Water Management

Byeong-Cheul Moon,Sung-Tae Park,Soon-Chul Kim,Suk-Ju Kwon,Andrew Martin Mortimer,Collin Piggin 韓國作物學會 1999 Korean journal of crop science Vol.44 No.1

The experiment was conducted to investigate emergence response of lowland weeds at different soil moisture contents, burying depths and upon changes in soil moisture. Rice germination was over 50% at all burying depths under aerobic condition, but the emergence rate of the soil surface placed seeds in saturated and flooded conditions decreased by 19% and 29%, respectively, as compared with that of aerobic condition. Rice seeds at burying depth of over 3 cm did not emerge at all. The emergence rate of Echinochloa crus-galli (L.) Beauv. in aerobic condition was lower than 30%, but the emergence pattern of E. crus galli (L.) Beauv. at different soil moisture contents and seeding depths was similar to that of rice. Emergence behavior of lschaemum rugosum Salisb., Ludwigia octovalvis (Jacq.) Raven and Sphenoclea zeylanica Gaertn. which are dominant lowland weed species in the Philippines also differed depending on soil moisture conditions and burying depths. lschaemum rugosum Salisb. emerged at all burying depths under aerobic condition, whereasLudwigia octovalvis (Jacq.) Raven emerged only at 0 cm deep under saturated and aerobic condition and Sphenoclea zeylanica Gaertn. at 0 cm deep under flooding condition. Weed seeds planted at 1, 3, and 5 cm deep in continuous flooded and saturated condition did not emerge at all, but upon a change of soil moisture condition from saturated to drainage (S~rightarrow D) and flooded to drainage (F~rightarrow D), grass weeds began to germinate again and the average emergence rate in S~rightarrow D and F~rightarrow D were 26% and 5% forE. crus-galIi (L.) Beauv., 9% and 8% forI. rugosum SaIisb., respectively. Weed seeds buried in soil in the pot showed great emergence at S~rightarrow D but did not emerge under continuous flooded condition. The diversity index accounting for dominance degree and occurrence aspect of weed, was the lowest at F~rightarrow D.

Arabidopsis Annexin1 Mediates the Radical-Activated Plasma Membrane Ca²⁺- and K⁺-Permeable Conductance in Root Cells

Laohavisit, A.,Shang, Z.,Rubio, L.,Cuin, T.A.,Very, A.-A.,Wang, A.,Mortimer, J.C.,Macpherson, N.,Coxon, K.M.,Battey, N.H. AMERICAN SOCIETY OF PLANT BIOLOGISTS 2012 The Plant cell Vol.24 No.4

A Survey of Termitomyces (Lyophyllaceae, Agaricales), Including a New Species, from a Subtropical Forest in Xishuangbanna, China

( Lei Ye ),( Samantha C. Karunarathna ),( Huli Li ),( Jianchu Xu ),( Kevin D. Hyde ),( Peter E. Mortimer ) 한국균학회 2019 Mycobiology Vol.47 No.4

A survey of mushrooms was conducted in Xishuangbanna, Yunnan Province, China, in the rainy season (May to October) of 2012, 2013, and 2014, during which 16 specimens of Termitomyces were collected. Preliminary macro- and micro-characteristics, together with ITS sequence data, showed that four of the specimens belonged to a new species (Termitomyces fragilis), while the other 12 belonged to T. aurantiacus, T. eurrhizus, T. globules, T. microcarpus, and T. bulborhizus. In this paper, T. fragilis is introduced as a species new to science based on morphological characterization and phylogenetic analyses. Macro- and micro- morphological descriptions, color photographs and line drawings of the new species, and a phylogenetic tree to show the placement of the new species are provided. T. fragilis is then compared with other closely related taxa in the genus Termitomyces.