백서에서 L - Tryptophan 의 과량 투여시 간장내 Triglyceride 축적에 미치는 호르몬의 효과

조무연,김윤수 ( Moo Youn Cho,Yoon Soo Kim ) 생화학분자생물학회 1978 BMB Reports Vol.11 No.1

When an excess amount of L-tryptophan (0.34mmole) was administered intraperitioneally to a normal rat the triglyceride content in the liver increased two times that of the normal control in three hours, but no significant changes of triglyceride content in the liver of adrenalectomized rat was noted, however, the triglyceride content in liver and FFA in the plasma of adrenalectomized rat administered epinephrine and L-tryptophan together was increased as much as one and a half times than that of the control adrenalectomized rat which received L-tryptophan or epinephrine only. No effects of hydrocortisone, glucagon and insulin on the triglyceride accumulation in the liver were noted in adrenalectomized rat receiving each hormone or L-tryptophan only, or hormone and L-tryptophan together. Therefore, it was confirmed that the triglyceride accumulation in the liver induced by L-tryptophan is due to the indirect effect of L-tryptophan through stimulation of epinephrine secretion.

Dehydro - L - ascorbic Acid 가 백서 간에서 Vitamin A 대사에 미치는 영향

조무연,김현옥,송정석 ( Moo Youn Cho,Hyun Ock Kim,Chung Suk Song ) 생화학분자생물학회 1976 BMB Reports Vol.9 No.2

The effect of dehydro-L-ascorbic acid (DHA) on the transfer of vitamin A to the liver was observed in the isolated rat liver perfusion. DHA had no effect on the transfer of vitamin A to the liver. The addition of DHA to the perfusate did not raise the level of either oxidized or reduced form of vitamin A in the liver. From this fact, DHA seems to be destroyed as soon as it is absorbed into the liver or not to be absorbed to the liver and rather to be destroyed in the blood.

Genomic changes of c-myc, c-H-ras in benzo(a)pyrene and dimethylbenz(a)anthracene treated human lymphoblast NC-37 cells

조무연,어완규,이상욱,정인철,Cho, Moo Youn,Eo, Wan Kyu,Lee, Sang Uk,Jeong, In cheol Korean Society of Life Science 1995 생명과학회지 Vol.5 No.3

To investigate genomic changes in c-myc gene by a chemical carcinogen, human lymphoblast NC-37 cells were exposed to benzo(a)pyrene(BP) and dimethylbenzanthracene(DMBA), and the c-myc gene expression was evaluated by Northern and Southern blot hybridization techniques. The results are as follows: When the genomic DNA of NC-37 cells exposed to several concentrations(1.25, 2.5 and 5ug/ml) of BP concentration. However, the c-myc gene was most significantly enhanced with 2.5ug/ml of BP. The expressions of c-myc gene in NC-37 cells was stimulated by BP and DMBA. Addition of TPA reduced the gene expression BP-treated cells, whereas it enhanced the gene expression in DMBA-treated cells. The expression of c-H-ras gene was slightly increased by treatment with BP and DMBA alone and in combination with TPA, however the magnitude of increase was not significantly different between each other. The expressions of c-myc c-H-ras genes in Burkitt's lymphoma cells were greater than those in NC-37 cells. When the DNA extracted from NC-37 cells exposed to various concentrations of BP were amplified by polymerase chain reaction using a primer set containing c-myc exon I, the amplified products were of the same size in all groups. To evaluate the BP toxicity in E.coli to which human c-myc gene-cloned pBR322 vector was inserted, Southern blot hybridization was conducted on c-myc genes digested with EcoRI/HindIII and Smal/Xbal restriction enzymes, and observing that in 2 ug/ml BP-treated cells a 3.5kb fragment was generated in addition to 1.3kb fragment which can be observed in normal cells. Direct nucleotide sequence analysis of polymerase chain reaction products showed a mutation of G$\longrightarrow$A transition at the Smal recognition site.

식이조성을 달리한 백서에 만성 알콜투여가 간장조직 내 malic enzyme 및 ATP-citrate lyase활성에 미치는 실험적 연구

조무연,김윤수,Cho, Moo-Youn,Kim, Yoon-Soo 생화학분자생물학회 1978 한국생화학회지 Vol.11 No.1

간장조직 내 lipogenic enzyme인 malic enzyme과 ATP-citrate lyase의 활성이 식이조성을 달리한 사료로 사육한 백서에서 어떻게 영향을 받으며 이동안 만성알콜투여 (6.7Cal/day)로 오는 효소활성 및 간장조직 내 지방 형성과 어떠한 상관 관계를 가지는가 관찰한 바 식이조성 차이로 오는 효소 활성은 고함수탄소 식이군에서 malic enzyme 활성만이 높았고 고지방식이군, 저함수탄소 식이군에서는 정상식이군에 비해 감소하고 알콜투여에 의한 영향은 볼 수 없었다. 간장조직내 식이에 의한 triglyceride 함량의 변화는 정상식이군에 비해 고지방식이군, 저함수탄소식이군에서는 높으나 알콜투여에 의한 효과는 고지방식이군에서만 현저한 증가를 나타냈다. 혈청내 유리지방산 농도는 알콜투여에 의해 정상식이군에서는 1.5배(倍), 저함수탄소식이군에서는 3배(倍)의 증가가 있었으며 고지방식이군에서는 차이가 없으며 고함수탄소식이군에서는 알콜투여군이 대조군에 비해 오히려 낮았다. 식이 조성및 알콜투여에 의한 혈청내 Phospholipid 함량에는 변화가 없었다. Influences of diet composition on the activities of two lipogenic enzymes, malic enzyme and ATP-citrate lyase, and on the hepatic triglyceride accumlation were investigated in rats. In addition, the effect of chronic administration of ethanol on these enzymes and hepatic triglyceride accumulation were studied. The results were summarized as fallowing. 1) The activity of malic enzyme in the liver of rat fed high-fat (HF), low-carbohydrate (LC) and high-carbohydrate (HC) diet groups was 62%, 55% and 155% of that of the normal level, and the ATP-citrate lyase activity was 22%, 31% and 44% of that of normal level, respectively. These changes of the lipogenic enzyme activities were not altered by chronic administration of ethanol to the rats. 2) Average content of triglyceride in the liver of rat in normal diet group was 9.27mg per g. of liver, 16.37mg in the normal diet plus ethanol treated group, 37.79mg in the HF diet group, 75.7mg in the HF plus ethanol treated group, 14.78mg in the LC diet group, 17.87mg in the LC diet plus ethanol treated group, 11.61mg in the HC group, 14.92mg in the LC diet plus ethanol treated group. These results, shows that the administration of ethanol was increased hepatic triglyceride content only in the high fat diet group. 3) Free fatty acid concentration was increased in the serum of rats free fatty acid concentration was decreased by 40% in the high carbohydrate group, may be due to the increased or decreased mobilization of lipid from peripheral adipose tissue. 4) No total phospholipid content in the serum of rat was observed among above different diet and plus alcohol treated group. It is therefore apparent that ethanol administration induces the hepatic accumulation of triglyceride in the HF diet group, whereas it reduces the hepatic accumulation of triglyceride and serum free fatty acid level in the HC diet group.

백서에서 L-Tryptophan의 과량 투여시 간장내 Triglyceride 축적에 미치는 호르몬의 효과

조무연,김윤수,Cho, Moo-Youn,Kim, Yoon-Soo 생화학분자생물학회 1978 한국생화학회지 Vol.11 No.1

L-tryptophan을 백서 복강내 과량 (0.34m mole) 투여시 간장내 지방축적에 있어서 호르몬의 작용을 관찰한 바 부신적출한 백서에서 Epinephrine과 L-tryptophan 동시주사군이 Epinephrine 단독주사군에 비하여 1.5배의 증가를 보이고 혈액내 유리지방산의 농도도 높으나 그외의 다른 호르몬(Hydrocortisone, Glucagon, Insulin)은 간장조직내 지방축적에 아무 작용도 없었다. When an excess amount of L-tryptophan (0.34mmole) was administered intraperitioneally to a normal rat the triglyceride content in the liver increased two times that of the normal control in three hours, but no significant changes of triglyceride content in the liver of adrenalectomized rat was noted, however, the triglyceride content in liver and FFA in the plasma of adrenalectomized rat administered epinephrine and L-tryptophan together was increased as much as one and a half times than that of the control adrenalectomized rat which received L-tryptophan or epinephrine only. No effects of hydrocortisone, glucagon and insulin on the triglyceride accumulation in the liver were noted in adrenalectomized rat receiving each hormone or L-tryptophan only, or hormone and L-tryptophan together. Therefore, it was confirmed that the triglyceride accumulation in the liver induced by L-tryptophan is due to the indirect effect of L-tryptophan through stimulation of epinephrine secretion.

식이조성을 달리한 백서에 만성 알콜투여가 간장조직내 malic enzyme 및 ATP - citrate lyase 활성에 미치는 실험적 연구

조무연,김윤수 ( Moo Youn Cho,Yoon Soo Kim ) 생화학분자생물학회 1978 BMB Reports Vol.11 No.1

Influences of diet composition on the activities of two lipogenic enzymes, malic enzyme and ATP-citrate lyase, and on the hepatic triglyceride accumlation were investigated in rats. In addition, the effect of chronic administration of ethanol on these enzymes and hepatic triglyceride accumulation were studied. The results were summarized as following. 1) The activity of malic enzyme in the liver of rat fed hibh-fat (HF), low-carbohydrate (LC) and high-carbohydrate (HC) diet groups was 62%, 55% and 155% of that of the normal level, and the ATP-citrate lyase activity was 22%, 31% and 44% of that of normal level, respectively. These changes of the lipogenic enzyme activities were not altered by chronic administration of ethanol to the rats. 2) Average content of triglyceride in the liver of rat in normal diet group was 9.27㎎ per g. of liver, 16.37㎎ in the normal diet plus ethanol treated group, 37.79㎎ in the HF diet group, 75.7㎎ in the HF plus ethanol treai:ed group, 14.78㎎ in the LC diet group, 17.87㎎ in the LC diet plus ethanol treated group, 11.61㎎ in the HC group, 14.92㎎ in the LC diet plus ethanol treated group. These results, shows that the administration of ethanol was increased hepatic triglyceride content only in the high fat diet group. 3) Free fatty acid concentration was increased in the serum of rats free fatty acid concentration was decreased by 40% in the high carbohydrate group, may be due to the increased or decreased mobilization of lipid from peripheral adipose tissue. 4) No total phospholipid content in the serum of rat was observed among above different diet and plus alcohol treated group. It is therefore apparent that ethanol administration induces the hepatic accumulation of triglyceride in the HF diet group, whereas it reduces the hepatic accumulation of triglyceride and serum free fatty acid level in the HC diet group.

HL-60 세포의 유전자 발현 및 topoisomerase의 기능 활성에 미치는 억제제의 영향

정인철(In Cheol Jeong),조무연(Moo Youn Cho),박장수(Jang Su Park) 한국생명과학회 2008 생명과학회지 Vol.18 No.1

인체 DNA topoisomerase는 DNA를 단일 또는 두 가닥을 일시적인 절단을 촉매하여 DNA의 topological 문제를 조절함으로써, DNA 복제, 전사, 재조합과 유사분열 과정 등에 관여한다. 이 효소는 많은 항생, 항암제의 표적효소로서 널리 알려져 있으며, 이들 유도체를 이용한 다양한 억제제의 개발과 임상적 응용에 관한 연구가 활발하게 진행되고 있다. 본 실험에서는 인체 백혈병 HL-60 세포에서 topoisomerase 억제제가 topoisomerase 기능 활성과 유전자 발현을 조절하는지를 규명하기 위하여 본 연구를 수행하였다. 연구 방법은 HL-60세포에 topoisomerase type Ⅰ과 type Ⅱ의 대표적 억제제인 10-hydroxycamptothecin (10-CPT)과 doxorubicin을 투여한 후 total RNA를 분리하였고, 10K-oligonucleotide microarray 방법으로 분석하여 유전자의 발현 양상을 조사하였다. 연구 결과에 의하면 10-CPT 또는 doxorubicin을 투여한 HL-60세포에서의 유전자 발현 양상은 주로 signal transduction, cell adhesion, cell cycle, cell growth, cell proliferation, cell differentiation, transcription 및 immune response 등과 관련이 있었다. Topoisomerase type Ⅰ의 억제제인 10-CPT를 HL-60 세포주에 투여 하였을 때 type Ⅰ으로 분류되는 topoisomerase Ⅲα, Ⅲβ 및 Ⅰ의 발현은 증가하였으나 type Ⅱ인 topoisomerase Ⅱα와 Ⅱβ의 유전자의 발현은 감소되었다. 반대로 type Ⅱ의 억제제인 doxorubicin을 투여하였을 때는 앞의 결과와 상반된 topoisomerase Ⅱα와 Ⅱβ의 유전자의 발현이 현저히 증가되었으며, topoisomerase Ⅲα와 Ⅲβ의 mRNA의 발현은 약간 감소하는 양상을 보였으나 의미 있는 차이는 없었다. 이 연구 결과는 앞으로 항암제의 기전을 밝히고 약물에 대한 치료 반응을 예측하고 새로운 약제 개발에 기초자료가 될 것으로 여겨진다. This studies were designed to elucidate whether inhibitors of topoisomerase regulate function and activity of topoisomerase, and gene expression in HL-60 human leukemia cells. HL-60 cells were treated with 10-hydroxycamptothecin or doxorubicin, total RNA was isolated, and expressed genes were investigated with human oligonucleotide microarray containing 10K gene, respectively. Expression profiles of the human leukemia HL-60 cells treated with 10-hydroxycamptothecin (10-CPT) or doxorubicin associated with signal transduction, cell adhesion, cell cycle, cell growth, cell proliferation, cell differentiation, transcription and immune response, especially genes related with transcription and cell growth. In HL-60 cells treated with 10-CPT, the expression of topoisomerase Ⅲα, Ⅲβ and Ⅰ gene from oligo chip microarray analysis were increased over, but the expression of topoisomerase IIα and IIβ gene were decreased over. In contrast, the expression of topoisomerase Ⅱα and Ⅱβ gene were increased over in HL-60 cells treated with doxorubicin, whereas the expression of topoisomerase Ⅲα and Ⅲβ mRNA remained no significant change. These results suggest that these data may be useful for novel therapeutic markers.

Distribution of Selenium Contents in Human Blood and Foods Produced in Korea

Sang-Hwan Oh(오상환),Moo-Youn Cho(조무연) 韓國營養學會 1983 Journal of Nutrition and Health Vol.16 No.3

위암의 Lauren씨 유형에 따른 위액의 산도, 세균 및 아질산염 농도에 관한 연구

최경현(Kyung Hyun Choi),장명웅(Myung Woong Chang),조무연(Moo Youn Cho),장희경(Hee Kyung Chang),이상호(Sang Ho Lee) 대한외과학회 1996 Annals of Surgical Treatment and Research(ASRT) Vol.50 No.5

Doxorubicin이 HL-60 사람 백혈병 세포내 topoisomerase의 활성과 발현에 미치는 영향

조무연,정인철 고신대학교 의학부 2004 高神大學校 醫學部 論文集 Vol.19 No.1

Background DNA topoisomerases are essential enzymes present in all organisms. They modify DNA topology in connection with a number of nuclear processes, such as replication, transcription, chromatin remodeling, chromatin condensation/decondensation, recombination and repair. In the strand-breakage reaction by a DNA topoisomerase, a tyrosyl oxygen of the enzyme attacks a DNA phosphorus, forming a covalent phosphotyrosine link and breaking a DNA phosphodiester bond at the same time. DNA topoisomerase have been shown to be the molecular targets of many antimicrobial and anticancer agents. Among topoisomerase-targeting drugs in clinical use at present, most act by trapping the covalent DNA-enzyme intermediates to convert a normal cellular enzyme to a DNA damaging agent. This studies were designed to elucidate whether pretreatment of HL-60 human leukemia cells with the topoisomerase I - directed drug doxorubicin would increase the expression of topoisomerase and to investigate the activity of topoisomerase mediated by doxorubicin in nuclear extract from HL-60 human leukemia cells. Methods We have conducted experiments on topoisomerase assay using gel electrophoresis, pUC-X I cloning, DNA sequencing, cell cytotoxicity in drug-treated cells, topoisomerase purification, quantitative RT-PCR analysis, northern blotting techniques, respectively. Results Doxorubicin inhibited the relaxation activity of topoisomerase in pUC19 DNA at warious concentrations (0.4-50 μM), while it enhanced the cleavage of topoisomerase in the pUC-X I by forming a cleavable complex at 0.4-2μM. The levels of the topoisomerase IIα mRNA from RT-PCR analysis and northern blot were increased in HL-60 cells treated with doxorubicin, whereas the expression of topoisomerase I and IIβ mRNA from RT-PCR analysis remained no significant change. Conclusion Our results suggest that overexpression of topoisomerase IIα mRNA by topoisomerase II-directed drug treatment is due to stabilizing drug-enzyme-DNA "cleavable complex".