http://chineseinput.net/에서 pinyin(병음)방식으로 중국어를 변환할 수 있습니다.
변환된 중국어를 복사하여 사용하시면 됩니다.
Padmanaban Mohanan,허준,김선오,나주련,김유진,이현아,김민경,양덕천 한국식물생명공학회 2019 Plant biotechnology reports Vol.13 No.3
The traditional oriental medicine bokbunja, prepared from immature berries of Rubus coreanus is used as an anti-oxidant, diuretic, and cure for impotence. The bokbunja wine made from fermented fruits of bokbunja has been used as a functional food as well. However, the usage of bokbunja has been problematic over the years due to the abundance of mistakenly identified berries such as Rubus chingii, Rubus crataegifolius, and Rubus occidentalis. Thus, here we developed a method for the molecular differentiation of Rubus species as well as the authentication bokbunja from other Rubus species. We screened several sequences from the chloroplast DNA of these species and found that the rpl16 region was polymorphic for R. coreanus and R. occidentalis, while the trnG–trnS intergenic spacer region was polymorphic for R. chingii and R. crataegifolius. Species-specific primers were designed and a multiplex PCR was performed by combining the markers at the rpl16 and trnG–trnS regions. Amplicons of 686 bp for R. coreanus and 478 bp for R. occidentalis were produced by the primers 5′ Rcor or 5′ Rocci, respectively, with 3′ rpl16; whereas, amplicons of 389 bp for Rubus crataegifolius and 180 bp for R. chingii were produced by 5′ Rcra or 5′ trnG–trnS, respectively, and 3′ Rcra/Rchi. The deduced molecular markers were utilized to authenticate the bokbunja products and demonstrated that the majority of bokbunja samples from the markets were adulterant berries. Hence, our results indicate that the produced molecular markers can serve as an effective tool to authenticate bokbunja.
인삼의 Flowering Locus T의 동종 유전자 탐구 및 개화시기 조절
Padmanaban Mohanan,Davajargal Myagmarav,김유진,양덕춘 한국약용작물학회 2018 한국약용작물학술대회 발표집 Vol.2018 No.10
Background : Panax ginseng Meyer, the king of Oriental medicine is a geophytic perennial shrub. P. ginseng possess long juvenile period which spans for at least 3-years before it could attain reproductive competence. To explore the significance of flowering time genes we isolated, characterized the Flowering Locus T (FT) gene and named as PgFT1 and PgFT2 to understand their role in ginseng floral transition. Methods and Results : By using extensive cloning and bioinformatics analysis we identified two FT homologs from ginseng a. The deduced PgFT sequences has a considerable homology to established FT homologs with the essential amino acids of a florigen function conserved. The tissue specific expression profiling by Real-time qPCR from the juvenile (2-years-old) and adult (4-year-old) ginseng plants have shown a spatial and temporal expression pattern, furthermore PgFT transcripts exhibited photoperiod dependent oscillation as well. Besides, PgFT1 and PgFT2 have shown an age specific expression pattern, in particular the transcripts were concentrated to the roots of P. ginseng. The ectopic expression of PgFT homologs in Arabidopsis thaliana induce precocious flowering by altering several floral pathway integrators, in addition PgFT1 transgenic Arabidopsis seedlings had an increased root length while, the influence of PgFT2 in transgenic seedlings apart from flowering is as yet to be revealed. Conclusion : Based on our results, we alleged the spatial and temporal expression of PgFT homologs could be significant in the flowering time transition of ginseng and their age specific expression pattern suggests the roots of ginseng are influential in determining the reproductive competency. On top of it, results from transgenic studies in Arabidopsis claimed that PgFT1 might involve in the underground organ development. Albeit, their molecular regulation, developmental role and the manipulation of PgFTs in ginseng flowering warranties further investigation.
Mohanan Padmanaban,Yang Tae-Jin,Song Young Hun 한국응용생명화학회 2023 Applied Biological Chemistry (Appl Biol Chem) Vol.66 No.-
Ginsenosides are the most valuable and pharmacologically active triterpenoid saponins found in Panax ginseng. Although light quality affects ginsenoside content, little is known about the underlying genetic and regulatory mechanisms. Additionally, the correlation between the adaptability of ginseng to shade and ginsenoside biosynthesis remains poorly understood. In the present study, transcriptome analysis of ginseng seedlings using RNA sequencing revealed that the expression of ginsenoside biosynthesis genes, including PgHMGR, PgFPS, PgSS, and PgUGT, was enhanced in shade conditions but downregulated by red light, indicating that far-red light might play an essential role in ginsenoside production. Further, gene expression analysis in adventitious roots and 2-year-old plants using qRT-PCR showed that the light quality-mediated expression patterns of ginsenoside genes varied with tissue and age. However, unlike the transcriptome, there was no difference in the total ginsenoside content in seedlings among various light conditions. Nevertheless, the amount of major protopanaxadiol-type ginsenosides increased under shade and red light conditions. Unlike seedlings and adventitious roots, there was a decrease in the expression of PgHMGR, PgFPS, PgSS, and PgDDS in 2-year-old plants, along with an increase in the ginsenoside content, under far-red light. Taken together, our findings suggest that far-red light is an important environmental factor for ginsenoside biosynthesis and diversification and provide information that can improve the quality of ginseng produced for medicinal purposes.
Development of SNP Marker to Authenticate Different Species of Sea Buckthorn by Multiplex ARMS-PCR
Padmanaban Mohanan(모하난 파드마나반),Jong Chan Ahn(안종찬),Hong Tao Wang(왕홍도),Gokulanathan Anandapadmanaban(아난다파드마나반 고클라나탄),Hyun A Lee(이현아),Yu Jin Kim(김유진),Doek Chun Yang(양덕춘) 한국약용작물학회 2017 한국약용작물학회 학술대회논문집 Vol.2017 No.2
Molecular signaling of ginsenosides Rb1, Rg1, and Rg3 and their mode of actions
Mohanan, Padmanaban,Subramaniyam, Sathiyamoorthy,Mathiyalagan, Ramya,Yang, Deok-Chun The Korean Society of Ginseng 2018 Journal of Ginseng Research Vol.42 No.2
Ginseng has gained its popularity as an adaptogen since ancient days because of its triterpenoid saponins, known as ginsenosides. These triterpenoid saponins are unique and classified as protopanaxatriol and protopanaxadiol saponins based on their glycosylation patterns. They play many protective roles in humans and are under intense research as various groups continue to study their efficacy at the molecular level in various disorders. Ginsenosides Rb1 and Rg1 are the most abundant ginsenosides present in ginseng roots, and they confer the pharmacological properties of the plant, whereas ginsenoside Rg3 is abundantly present in Korean Red Ginseng preparation, which is highly known for its anticancer effects. These ginsenosides have a unique mode of action in modulating various signaling cascades and networks in different tissues. Their effect depends on the bioavailability and the physiological status of the cell. Mostly they amplify the response by stimulating phosphotidylinositol-4,5-bisphosphate 3-kinase/protein kinase B pathway, caspase-3/caspase-9-mediated apoptotic pathway, adenosine monophosphate-activated protein kinase, and nuclear factor kappa-light-chain-enhancer of activated B cells signaling. Furthermore, they trigger receptors such as estrogen receptor, glucocorticoid receptor, and N-methyl-$\text\tiny{D}$-aspartate receptor. This review critically evaluates the signaling pathways attenuated by ginsenosides Rb1, Rg1, and Rg3 in various tissues with emphasis on cancer, diabetes, cardiovascular diseases, and neurodegenerative disorders.
진세노사이드 고 함유 개체 육성을 위한 고려인삼 우수 0837계통과 미국삼의 종간교잡
서광훈,양동욱,Padmanaban Mohanan,김유진,권우생,안종찬,박진규,양덕춘 한국약용작물학회 2018 한국약용작물학술대회 발표집 Vol.2018 No.10
Background : Panax ginseng the king of Oriental medicines, has enormous potential against many of the disease symptoms and ailments. Panax quinquefolius is an another important member of Panax family which has similar benefits and medicinal properties. Both the species contains ginsenosides a pharmacologically active component abundant in their roots. In an effort to establish the new ginseng cultivars with improved agronomical characters such as root shape, stress tolerant and higher ginsenoside contents, the reciprocal interspecific hybrids were generated Methods and Results : Four elite cultivars and two new lines of ginseng were chosen to produce interspecies reciprocal hybrids with P. quinquefolius, by pollen dusting on the emasculated flowers. Among the F1 populations, the reciprocal hybrids generated by using new line 0837 both as maternal and paternal parent showed better properties and hybrid vigor. They showed strong root phenotypes with many lateral and fine roots thus having 10% to 20% higher ginsenoside contents compared to the parental populations. Among the major ginsenosides, the pharmacologically active ginsenosides such as Re, Rb1, Rb2 and F1 were enriched and accounted for the 70% of the PPD ginsenosides in 0837/Pq and Pq/0837 crosses, displaying strong dominance. Conclusion : Thus, based on our result we could conclude that P. ginseng line 0837 is the superior variety compared to the already existing lines for performing the interspecific hybrids with promising outcome in their root quality and ginsenoside content.
Kwang-Hoon Seo,Padmanaban Mohanan,Dong-Uk Yang,Yu Jin Kim,Woo-Saeng Kwon,DEOK-CHUN YANG 한국원예학회 2019 Horticulture, Environment, and Biotechnology Vol.60 No.4
Panax ginseng and Panax quinquefolius are important herbs with similar benefi ts and medicinal properties. Both possess ginsenosides, the pharmacologically active components, abundantly in their roots. In an attempt to establish ginseng cultivars with improved agronomical characters and higher ginsenoside content, reciprocal interspecifi c hybrids were generated by artifi cial pollination of emasculated fl owers. Three elite cultivars of P. ginseng , types Jakyung, Chungkyung, and Hwangsook, and two new lines, 0837 and 0702, were used to assess interspecifi c hybrid potential. Among F 1 reciprocal hybrids generated using line 0837 as either the maternal or paternal parent showed excellent agronomical traits and hybrid vigor. They showed strong root phenotypes with many lateral and fi ne roots resulting in 10 to 20% higher ginsenoside content compared to the parental lines. Among the major ginsenosides, pharmacologically active Re, Rb1, Rb2, and F1 were enriched. They accounted for 70% of the protopanaxadiol ginsenosides in 0837/ Pq and Pq /0837 crosses, displaying strong maternal dominance. Thus, based on our results, we conclude that P. ginseng line 0837 is superior compared to already existing lines for performing interspecifi c hybridization with promising outcomes in root quality and ginsenoside content.