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        Evaluation of Genetic Relationships among Some Persian Cultivated and a Wild Pomegranate Accessions Using RAPDs and SSRs Molecular Markers

        Abdolkarim Zarei,Zabihollah Zamani,Mohammad Reza Fatahi Moghadam 한국원예학회 2009 Horticulture, Environment, and Biotechnology Vol.50 No.3

        Twenty-eight cultivated pomegranates, one hybrid seedling and one wild accession from three provinces (five eco-geographical regions) of Iran were used for the determination of their genetic relationships by using RAPD markers. Sixty-seven RAPD decamer primers were screened, out of which twenty were amplified good, reproducible and polymorphic fragments among accessions. From a total of 309 recorded fragments, 174 were polymorphic between accessions (57.64%). The Jaccard’s similarity coefficients ranged from 0.15 (between the wild accession and Poost-Sefide-Torsh cv.) to 0.95 (between ‘Shirin-Shahvar’ and ‘Shirine-Hastei’). The collective resolving power (Rp) rate value of 36.08 was scored for all RAPD primer bands. UPGMA dendrogram clustered the accessions in agreement with their geographical origins. In addition, nine SSRs loci were examined on these accessions that only one of them was polymorphic. This study revealed that the wild accession of pomegranate was genetically different from domesticated ones, indicating that wild genotypes are worthy for consideration in breeding programs of pomegranate.

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        Assessment of Morphological Variation between Some Iranian Wild Cerasus Sub-genus Genotypes

        Ali Shahi-Gharahlar,Zabihollah Zamani,Mohammad Reza Fatahi,Naser Bouzari 한국원예학회 2010 Horticulture, Environment, and Biotechnology Vol.51 No.4

        Iran is one of the important countries for the distribution of Cerasus subgenus species with around 225,000 tons production of cherries. Together with Turkey and USA it has been one among the three leading producers of these fruits. One of the important aims in cherry breeding is developing dwarfing rootstocks with good adaptation with different climates and resistance to biotic and abiotic stresses. A program has been initialized in Iran for breeding and developing new rootstocks for cherries, suitable for climatic and edaphic condition of this country. The present study was conducted under this program to categorize some Cerasus sub-genus wild genotypes and improved cultivars and rootstocks, by using vegetative characters. Conservation of the genetic resources of these species is another important aim of this program. Around 27 morphological parameters were evaluated on 74 genotypes in seven species of this sub-genus from eight provinces of Iran. These consisted of P. avium L., P. cerasus L., P. mahaleb L., P. incana Pall., P. brachypetala Boiss., P. pseudoprostrata Pojark. and P. microcarpa Bioss. Analysis of variance of the studied parameters was significant among genotypes, showing a high level of variability for the characters. Results of simple correlation analysis showed the existence of significant positive and negative correlations among some important parameters such as tree height, tree growth habit, trunk diameter, internode length, growth compactness and leaf area. Also, factor analysis showed that tree growth habit, height, growth compactness, trunk diameter, internode length, pubescence on the leaf upper surface and number of leaf serration per cm constitute the main factors. Effective characters were categorized within six factors and accounted for 77.74 of overall variance. Cluster analysis was performed using these six factors and genotypes in the approximate distance of 13 were divided into six main groups. These groups were divided into several sub-clusters and improved varieties or cultivars were clearly separated from the others. The P. cerasus cultivars and genotypes together with one genotype of the P. avium and improved rootstocks (‘Gisela6’ and ‘Colt’) located in one sub-cluster. Scatter plot analysis using two main factors could also clearly separate the location of genotypes and verified genotypes from each other.

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