Comparison of Melanoma Subtypes among Korean Patients by Morphologic Features and Ultraviolet Exposure

( Hong Sun Jang ),( Jee Hung Kim ),( Kyu Hyun Park ),( Jae Seok Lee ),( Jung Min Bae ),( Byung Ho Oh ),( Sun Young Rha ),( Mi Ryung Roh ),( Kee Yang Chung ) 대한피부과학회 2014 Annals of Dermatology Vol.26 No.4

Background: Genetic alterations have been identified in melanomas according to different levels of sun exposure. Whereas the conventional morphology-based classification provides a clue for tumor growth and prognosis, the new classification by genetic alterations offers a basis for targeted therapy. Objective: The purpose of this study is to demonstrate the biological behavior of melanoma subtypes and compare the two classifications in the Korean population. Methods: A retrospective chart review was performed on patients found to have malignant melanoma in Severance Hospital from 2005 to 2012. Age, sex, location of the tumor, histologic subtype, tumor depth, ulceration, lymph node invasion, visceral organ metastasis, and overall survival were evaluated. Results: Of the 206 cases, the most common type was acral melanoma (n=94, 45.6%), followed by nonchronic sun damage-induced melanoma (n=43, 20.9%), and mucosal melanoma (n=40, 19.4%). Twenty-one patients (10.2%) had the chronic sun-damaged type, whereas eight patients (3.9%) had tumors of unknown primary origin. Lentigo maligna melanoma was newly classified as the chronic sun-damaged type, and acral lentiginous melanoma as the acral type. More than half of the superficial spreading melanomas were newly grouped as nonchronic sun-damaged melanomas, whereas nodular melanoma was rather evenly distributed. Conclusion: The distribution of melanomas was largely similar in both the morphology-based and sun exposure-based classifications, and in both classifications, mucosal melanoma had the worst 5-year survival owing to its tumor thickness and advanced stage at the time of diagnosis. (Ann Dermatol 26(4) 485~490, 2014)

열처리에 의한 Morganella morganii와 Photobacterium phosphoreum 유래 Histidine Decarboxylase의 불활성화

박원민(Won-Min Pak),김꽃봉우리(Koth-Bong-Woo-Ri Kim),김민지(Min-Ji Kim),박지혜(Ji-Hye Park),배난영(Nan-Young Bae),박선희(Sun-Hee Park),안동현(Dong-Hyun Ahn) 한국식품영양과학회 2016 한국식품영양과학회지 Vol.45 No.3

Histamine을 생성하는 Morganella morganii와 Photobacterium phosphoreum으로부터 crude histidine decarboxylase(HDC)를 추출하여 65~121°C로 열처리한 다음 균의 생육 및 효소 활성 변화에 대해 살펴보았다. 그 결과 M. morganii와 P. phosphoreum은 모든 열처리 조건에서 비 가열 처리구와 비교 시 균의 생육이 저해됨을 확인하였다. M. morganii와 P. phosphoreum 유래 HDC의 효소 활성은 65°C에서 90% 이상의 효소 활성이 저해됨을 확인하였고, 온도가 증가함에 따라 유의적으로 활성이 억제되는 것으로 나타났다. SDS-PAGE 결과에서는 65~100°C 범위에서 비가열 처리구와 비교 시 조효소액의 단백질 패턴의 변화가 크지 않았으나, 121°C에서 단백질 band의 강도가 크게 약해졌다. Native-PAGE에서는 65°C 처리 조건에서부터 단백질 패턴의 변화가 크게 나타났다. 따라서 가열처리(65~121°C)는 histamine 생성균인 M. morganii와 P. phosphoreum의 생육을 억제할 뿐만 아니라 HDC의 효소 활성도가 저해됨을 확인하여, 식품산업에서 적용되고 있는 열처리 조건에서 histamine 생성 억제에 큰 효과가 있는 것으로 생각한다. This study was performed to investigate the effects of various thermal treatments on the growth of Morganella morganii and Photobacterium phosphoreum and activity of crude histidine decarboxylase (HDC) obtained from M. morganii and P. phosphoreum. Crude HDC and the two strains were treated at 65°C/30 min, 80°C/10 min, 100°C/10 min, and 121°C/10 min. Activity of crude HDC decreased with increasing temperature. Viable cells counts of M. morganii and P. phosphoreum were not detected in any heated samples. SDS-PAGE patterns of heated HDC did not show significant differences up to 100°C. However, at 121°C, protein band intensity was weakened. In native-PAGE, there was a major change in the pattern of HDC at 65°C. These results suggest that thermal treatment can help to reduce histamine production by reducing HDC activity and growth of M. morganii and P. phosphoreum.

난치성 백반증에 대한 흡입물집을 이용한 비배양 표피세포이식술 20례

배정민 ( Jung Min Bae ),정한미 ( Han Mi Jung ),이한나 ( Han Na Lee ),이로우 ( Ro Woo Lee ),은성혜 ( Sung Hye Eun ),권혁선 ( Hyuck Sun Kwon ),이지혜 ( Ji Hae Lee ),김경문 ( Gyong Moon Kim ) 대한피부과학회 2018 대한피부과학회지 Vol.56 No.7

Background: As nonsurgical interventions for vitiligo are not always successful, various surgical modalities have been used in patients with refractory vitiligo. Of these, non-cultured epidermal suspension transplantation (NCES) was recently introduced to treat large recipient sites using cells from small donor tissue. Objective: We assessed the effectiveness and safety of NCES as a surgical treatment for patients with refractory vitiligo. Methods: We retrospectively reviewed 20 cases in 17 patients (11 females; median age 25 years) who underwent NCES from July 2015 through March 2018. Suction blisters (20 mm in diameter) were collected from the patient’s inner thigh at a donor-to-recipient area ratio of 1:5. After the addition of 5 mL recombinant trypsin solution to the suction blisters, followed by incubation at 37°C for 60 min, epidermal cells were manually scraped off the blister surface, and epidermal cell suspension was obtained by centrifugation at 1,500 RPM for 5 min. The suspension was applied to the vitiligo regions after epidermal ablation of those regions. Phototherapy resumed 1 month later. Treatment success was defined as ≥75% repigmentation of the surgical site, and all adverse events were noted. Results: Overall, 85.0% of cases (17/20) exhibited treatment success. Adverse events included hyperpigmentation (20%) and surgical site infection (5%), but the treatment was tolerable in all cases. Conclusion: NCES is a reliable surgical option for patients with vitiligo refractory to nonsurgical treatment. Large areas of vitiligo can be treated by NCES, and use of this technique should be encouraged in Korea. (Korean J Dermatol 2018;56(7):426∼432)

침습성 아스페르길루스증으로 치료받았던 급성 백혈병 환자에서 조혈모세포이식후 발생한 파급성 아스페르길루스증 1례

배기선,박지영,신수연,문영철,최희정,조민선,성주명 대한감염학회 2003 감염과 화학요법 Vol.35 No.4

본 증례는 침습성 아스페르길루스증의 과거력을 갖고 있는 백혈병환자에서 조혈모세포이식을 받은 후 치명적인 파급성 아스페르길루스증이 발병한 예이다. 환자는 항암요법 후 흉부 방사선 및 단층촬영에서 진균성 폐렴을 의심하여 항진균제를 투여하고 폐엽절제술을 시행하여 아스페르길루스에 의한 폐렴임을 확인하였다. Amphotericin B로 치료한 후 조혈모세포이식을 시행받은 뒤 치료에도 불구하고 파급성 아스페르길루스증으로 진행되어 사망하였다. 이 증례에서 보듯이 이전에 아스페르길루스증의 과거력이 있는 경우에 이식후 치명적인 결과를 유발할 수 있으므로, 고위험군을 선별하는 지침과 적절한 치료법에 대한 연구가 필요하겠다. Invasive aspergillosis has been increasing as the number of severe immunocompromised hosts rises. Particularly, in allogeneic hematopoietic stem cell transplantation (HSCT) recipients, incidence of invasive aspergillosis ranges from 4 to 10%. Even with appropriate treatment, the prognosis of invasive aspergillosis in allogeneic HSCT recipients remains poor, showing high mortality rate. Herein. we report a case where invasive aspergillosis in a patient with acute myelogeneous leukemia progressed to disseminated aspergillosis after allogeneic HSCT. A 31-year-old woman with acute myelogenous leukemia had invasive aspergillosis after third reinduction chemotherapy. After administering amphotericin B, the patient underwent the wedge resection of lung. and HLA-matched allogeneic HSCT was then conducted. On day 14 of transplantation, the patient died of disseminated aspergillosis, including possible cerebritis and endocarditis despite the amphotericin B therapy.

노인에서의 외과 환자에 대한 임상적 연구

배진선,강민규,송인상 충남대학교 의과대학 지역사회의학연구소 1989 충남의대잡지 Vol.16 No.2

378 patients over 65 years old were reviewed, who were admitted during the period from June. 1. 1985 to December 31. 1988 in the Department of Surgery, Chungnam National University Hospital. The results were as followings: 1. The rate of patients over 65 years to all who were admitted during same period was 11.3%. 2. The number of male patients was 183, that of female 195, the overall ratio of male to female being 1:1.07. In benign diseases, that was 1:0.95, and in malignant diseases 1:1.26. 3. Hemoglobin value at admission was below 10.0gm% in 7.5% of patients with benign diseases and in 15.0% of patients with malignant diseases. 4. Serum albumin at admission was below 3.5gm% in 60% of patients with benign diseases and in 50% of patients with malignant diseases. 5. As to the distribution of benign diseases, disease in biliary system was most frequent(33%), followed by appendicitis(25%), ileus and trauma in jejunum and ileum(13%), and peptic ulcer(6%) in the order of frequency. 6. As to the distribution of malignant diseases, stomach cancer(51%) was most frequent, followed by colorectal cancer(25%), and hepatobiliary cancer(10%) in the order of frequency. 7. 47% of patients with benign diseases and 44% of that with malignant diseases had associated diseases, among which hepertension was most frequent, followed by lung disease, hearth disease, diabetes mellitus, liver disease and renal disease in the order of frequency. 8. Complications were most prevalent in the patients with hepatobiliary diseases, followed by appendicitis and colorectal cancer, and among complications, would infection was most frequent, followed by urinary tract infection and respiratory infection in the order of freguency. In the patients with colorectal cancer, mechanical lieus was second frequent complication after wound infection. 9. The mortality rate was highest in the patient with colorectal cancer(22.2%), followed by in those with benign biliary disease(10%), overall mortality rate being 4.3%. 10. Major causes of deaths were sepsis, respiratory failure, bile peritonitis and renal failure.

역해석법에 의한 침하 및 압밀도 분석

배종순,권민재,김민아,김동숙 경상대학교 생산기술연구소 2003 工學硏究院論文集 Vol.19 No.-

This study is the analysis for settlement and degree of consolidation with Compression Index(Cc) and Coefficient of Consolidation(Cv) that is changed due to Consolidation using the actual measurement through a back-analysis method based on the least squares method. We were try to decide the expected settlement and the time of filling removal by using the degree of consolidation which is the closest to the settlement behavior by actual measurement. At the ground improvement construction site proposed for Gwangyang container Port, we meet good results as follows; With regard to the settlement by actual measurement before the completion of loading filling, it is estimated that Barron's method(Sr/Sm=100.4%) is closer to the settlement by actual measurement than Hansbo method (Sr/Sm=78.8%), due to external influences such as immediate settlement or equipment load. After the completion of loading filling, while Asaoka's method (Sr/Sm=109.1%) and Barron's method(Sr/Sm=112.8%) were overvalued, Hyperbolic method(Sr/Sm=70.0%) was undervalued, and Hansbo method(97.6%) showed the most similar results to the settlement by actual measurement. The results of this study showed that, in applying the consolidation parameter obtained by means of back-analysis, Barron's method was the most appropriate before the completion of loading filling, while after it Hansbo's method was, with 99.4% of the settlement by actual measurement.

연약지반개량 전 후의 토질특성 변화분석

배종순,권민재 慶尙大學校 經營行政大學院 2004 工學硏究院論文集 Vol.20 No.-

Base on the subsurface exploration(contain physical & mechanical test) performed at each boring hole, we try to analyze the change of soil property after improvement ground. At this study, we try to find out relationship physical and mechanical characters at the agricultural center of Jangyu. We obtained relationship as follows; 1) water content and soil constant e=2.71×ω_(n)/100, γ_(t)=2.71(1+ω_(n)/100)γ_(w)/(1+2.71×ω_(n)/100) before improvement: WL=0.486 ω_(n)+26.88, PI=0.38 ω_(n)+6.08 after improvement: WL=0.781 ω_(n)+29.80, PI=0.45 ω_(n)+16.71 2) shear strength before improvement: C_(ub)=0.036 Z+0.09(UU, q_(u)), C_(ub)=0.007 Z+0.14(Vane) after improvement: C_(ua)=0.033 Z+0.49(UU, q_(u)), C_(ua)=0.015 Z+0.40(Vane) 3) Compression index C_(c)=0.013 (ω_(n)+5), C_(c)=0.53 (e_(o)) 4) OCR was increased to 2.3 from 1.2; C_(u)/ΔP=0.21

Effects of electroacupuncture on clod allodynia in a rat model of neuropathic pain : Mediation by spinal adrenergic and serotonergic receptors

Kim, Sun-Kwang,Park, Jung-Hyuk,Bae, Sang-JIn,Kim, JI-Hoon,Hwang, Byung-Gil,Min, Byung-Il,Park, Doing Suk,Na, Heung-Sik EAST-WEST MEDICAL RESEARCH INSTITUTE KYUNG HEE UNI 2005 東西醫學硏究所 論文集 Vol.2005 No.-

The present study was performed to examine the effects of electroacupuncture (EA) on cold allodynia and its mechanisms related to the spinal adrenergic and serotonergic systems in a rat model of neuropathic pain. For the neuropathic surgery, the right superior caudal trunk was resected at the level between S1 and S2 spinal nerves innervating the tail. Two weeks after the nerve injury. EA simulation (2 or 100 Hz) was delivered to Zusanli (ST36) for 30 min. The behavioral signs of cold allodynia were evaluated by the tail immersion test{i.c., immersing the tail in cole water (4℃) and measuring the latency to an abrupt tail movement} before and after the stimulation. And then, we examined the effects of intrathecal injection of prazosin (α₁-adrenoceptor antagonist, 30 ㎍), yohimbine (α₂-adrenoceptor antagonist, 30 ㎍), NAN-190 (5HT_(1A) antagonist, 15 ㎍), ketanserin (5-HT_(2A) antagonist, 30 ㎍), and MDL-72222 (5-HT_(3) antagonist, 12 ㎍) on the action of EA simulation. Although both 2 Hz and 100 Hz EA significantly relieved the cold allodynia signs, 2 Hz EA induced more robust effects than 100 Hz EA. In addition, intrathecal injection of yohimbine, NAN-190, and MDL-72222, but not prazosin and ketanserin, significantly blocked the relieving effects of 2 Hz EA on cold allodynia. These results suggest that low-frequency (2 Hz) EA is more suitable for the treatment of cold allodynia than high-frequency (100 Hz) EA. and spinal α₂-adrenergic, 5-HT_(3), but not α₁-adrenergic and 5-HT_(2A), receptors play important roles in mediating the relieving effects of 2 Hz EA on cold allodynia in neuropathic rats.

지방산 유지 원료에 따른 고형비누의 특성에 관한 연구

정명선,배한수,이봉연,민경혜,류덕환 계명대학교 생활과학연구소 2001 科學論集 Vol.27 No.-

The experimental results on the washing efficiency of detergent made from corn oil, wasted cooking oil and pig fat oil are as follows ; Laundry factors in this experiment are detergent concentration, laundry temperature and laundry time. 1.The surface tension of each detergent showed the lowest in 0.2% concentration corn oil 60.84, wasted cooking oil 61.11, pig fat oil 60.73dyne/cm. 2.The wasthing efficiency based on different concentration of detergents showed the highest in 0.2% concentration. 3.The highest washing efficiency appeared in 0.2% concentration 40℃ temperature and 40~50 min. time. There showed no difference in the washing efficiency according to the volume of water. It can be said that the frictional force of bearing rather than the volume of water worked on the efficiency.

자궁내막증 백서모형에서 Danazol과 Decapeptyl의 치료효과에 관한 연구

황선보,이민석,심재현,김동훈,배한익 동국대학교 경주대학 1996 東國論集 Vol.15 No.-

본 연구의 목적은 장간막에 자궁내막 조직을 자가이식하여 수술적으로 유도된 자궁내막증 백서모형에서 자궁내막증의 치료를 위해 danazol과 decapetyl의 치료효과를 평가하는 것이다. 백서(n=48)들은 무작위적으로 정상대조군(n=12)과 수술적으로 유도된 자궁내막증군(n=36)으로 구분하였다. 후자는 비치료군과, danazol 치료군과 decapeptyl 치료군으로 세분하였다(각 군의 n=12). Danazol(8mg/일)은 수술후 4째주부터 구강을 통해 투여되었다. Decapeptyl(0.2mg/2주)은 근주하였다. 처음투약후 10일, 20일, 40일, 60일째에 각군의 2마리의 쥐를 희생시켰다. 저자들은 자궁내막증 이식체와 난소의 크기를 측정하여 다음의 결론를 얻었다. 1. 자궁내막증 이식체의 크기는 비치료군에 비해(3.91±1.08mm) danazol 치료군과 decapeptyl치료군에서 (각각2.28±0.17mm 과 2.78±0.97mm), 현저히 감소되었다(p<0.01). 2. danazol과 decapeptyl 두 치료군에서 장기치료가 단기치료보다 자궁내막증 이식체의 크기를 감소시키는데 더 효과적이었다(p<0.01). 3. 난소의 크기는 정상대조군과, 비치료군과 decapeptyl 치료군에서 유의하게 차이를 보이지 않은데 반해(각각 4.46±0.85mm, 4.77±0.82mm과 4.97±0.92mm), danazol 치료군에서는 유의하게 감소하였다(3.97±0.63mm) (p<0.63mm). 백서모형에서 수술적으로 이식된 자궁내막조직의 성장과 발달은 자궁내막증의 병인 이해와 신약개발에 유용한 연구모델을 제공할 수 있을 것 이다. The purpose of this study was to surgically induce endometriosis in the rat by autotransplanting endometrial tissue to the mesentery and to evaluate the efficacy of danazol and decapeptyl for the treatment of endometriosis. Rats(n=48) were randomly assigned to normal control group(n=12) and surgically induced endometriosis group(n=36). The latter was further divided into the untreated, danazol treated and decapeptyl treated groups (n=12 for each group). Danazol(8mg/day) was orally administrated from 4weeks after operation. Decapeptyl(O.2ml/2weeks) was injected intramuscularly. Two rats of each group are sacrificed on the 10th, 20th, 40th, 60th, 80th and lOOth day after the first drug administration. We measured sizes of endometriotic implants and ovaries. The results were as follows; 1. The sizes of endometriotic implants were significantly decreased(P<O.Ol) in the danazol and decapeptyl treated groups (2.28±O.71mm and 2.78±O.89mm, respectively) compared with untreated group(3.91±l.08mm). 2. Long term treatment was more effective than short term treatment in decreasing the size of endometriotic implants in both danazol and decapeptyl treated groups(P<O.Ol). 3. The size of ovaries was not significantly different among the normal control, untreated and decapeptyl treated groups(4.65±O.83mm, 4.77±O.82mm and 4.97±O.92mm, respectively) but was significantly decreased(P<O.Ol) in the danazol treated group(3.97±O.63mm). The successful growth and development of surgically transplanted endometrial tissue in the rat may offer an useful research model that can be used to understand pathogenesis of endometriosis and to explore new drug therapies.