심근경색 모델 흰쥐에서 스트레스가 B-type Natriuretic Peptide 발현 및 심박변이율 변화에 미치는 영향

이삼윤(Sam Youn Lee),이미경(Mi Kyoung Lee),김남호(Nam-ho Kim),주민철(Min Cheol Joo),조항정(Hyang Jeong Jo),강지숙(Ji Sook Kang),김병숙(Byung Sook Kim),윤선식(Sun Sik Yoon),최을식(Eul Sig Choi),이문영(Moon Young Lee) 대한스트레스학회 2010 스트레스硏究 Vol.18 No.3

심박변이율의 감소는 심근 경색 환자에서 예후의 악화와 관련되어 있다. 심박변이율 중 very low frequency가 심부전환자의 예후를 판단할 수 있는 독립적 인자로서 사용이 가능하다고 보고된 바 있으며, 심실의 압력 증가에 반응해서 생성되는 B-type natriuretic peptide가 심근 경색의 예후 인자로서 사용할 수 있음 역시 보고된 바 있다. 본 연구에서는 심근경색 모델을 제작하여 일정의 회복 기간을 거친 다음 다시 일정 기간의 스트레스를 겪게 한 후 심박변이율의 변화 및 심실 조직에서의 BNP 발현 정도를 비교하여 스트레스에 의한 영향을 관찰하고자 하였다. Sprague-Dawley계 수컷 흰쥐 15마리를 대상으로 하였다. 실험동물은 인위적 스트레스 및 수술을 받지 않은 대조군(CON, n=5), 심근경색 수술을 받은 후 restraint stress를 받지 않은 군(MI+No Stress, n=5), 심근경색 수술을 받은 후 1주일 동안 restraint stress를 받은 군(MI+Stress, n=5)으로 분류하였다. 심근경색 모델 제작 직후 15분 이상 심전도의 변화를 기록하였으며, 2개월 정도 후 심근경색 수술을 받은 동물을 두 군으로 나누어 그 중 한 군에는 1주일 동안 restraint stress를 가한 후 restraint stress를 가하지 않은 동물과 심박변이율을 비교 분석하였다. 심실 조직은 좌심실 전벽 부위의 위축을 관찰할 수 있었고, 대식세포에 의하여 응고, 괴사된 심근세포의 탐식과 혈관이 풍부한 육아조직 및 섬유 변화를 볼 수 있었다. 심박변이율은 심근경색 수술을 받은 직후 심박수는 유의한 증가를 보였고, standard deviation of the normal to normal intervals (SDNN), very low frequency (VLF) 및 low frequency (LF)의 유의한 감소를 보였다. 심근경색 수술을 받았던 동물에서 두 달 정도의 회복 기간을 거친 후 스트레스를 받지 않은 군은 심박수 및 기타 심박변이율 분석에서 SDNN 값을 제외하고는 정상군과 유의한 차이를 관찰할 수 없었던 반면 1주일 동안 스트레스를 받은 군에서는 심박수가 다시 유의하게 증가했을 뿐만 아니라 SDNN, VLF 및 LF 역시 정상군과 유의한 차이를 보였다. 이상의 결과를 종합하면 심근경색 동물에 대해 1주일 동안 스트레스를 가한 결과 심박변이율이 감소하고 심실에서의 BNP 발현은 더욱 증가하여 악화함을 보여주고 있다. Diminished heart rate variability (HRV) is associated with less favorable prognosis after myocardial infarction (MI). It has been reported that very low frequency (VLF) power in HRV analysis is an independent risk predictor in patients with congestive heart failure and B-type natriuretic peptide (BNP) can be used as a prognostic factor of MI. The purpose of this study was to investigate whether stress has an effect to the changes of BNP expression and/or heart rate variability in MI model in rats. Male Sprague-Dawley rats were divided into 3 groups: control group (CON), MI group (MI+No Stress), and MI followed by stress group (MI+Stress). MI+Stress group rats were raised for a two month recovery period after the operation, followed by being exposed to restraint stress for 2 hours per day for 1 week. Electrocardiogram was recorded after the operation and the last day after 1 week of stress. The frequency components of HRV were calculated in the frequency domain such as VLF, low frequency (LF), high frequency (HF) and so on. In HRV analysis, standard deviation of the normal to normal intervals (SDNN) was significantly reduced in both groups compared to the control group. VLF and LF also were significantly reduced in MI+Stress group compared to the control groups. In addition, BNP expression in western blotting was shown the strongest bands in MI+ Stress group among experimental groups. These results suggest that BNP and HRV were aggravated by stress in MI rat model. (Korean J Str Res 2010;18:275∼285)

알코올의 섭취가 흰쥐의 혈액과 간의 지질대사에 미치는 영향

이명숙,김영연,김효숙,김매하,전진순,서진숙,송선미,양영재,윤원손,이미라,이효영,정승현 誠信女子大學校 生活文化硏究所 2004 生活文化硏究 Vol.18 No.-

To investigate the effects of ethanol on the lipid metabolism in blood and liver, twenty one male Sprague Dawley rats had orally taken the 25% alcohol and 50% alcohol by 5g per Kg of weight during 3 weeks after the adjusting period. For the energy balance, 85% sucrose and 45% sucrose was added in control group and 25% alcohol group each. The weight was reduced by alcohol treatment according to the levels, otherwise the weight of liver and heart was little increased in alcohol groups rather than in control without significance. Moreover, RBC, WBC, hemoglobin and hematocrit were increased by alcohol treatment according to the levels without significance. Otherwise, adjusted bilirubin levels by 100g of weight was significantly increased by alcohol treatment dose dependently. Alcohol induced RBC degradation and increased circulated bilirubin because of liver damage. For blood lipid profiles, blood cholesterol was increased in 50% of alcohol group compared to that of 25% and control groups, otherwise, blood triglycerides (TG) was increased by 30% in 25% alcohol groups rather than in control but not significantly different. The hepatic lipid composition, cholesterol levels in alcohol treatment groups was lower than those in control. The hepatic TG was not influenced by alcohol treatment. The two reasons of decreasing hepatic lipids were suggested by 85% sucrose of control for energy balance more synthesized cholesterol and TG than those in 25 or 50% alcohol groups, and by cholesterol and TG could not be synthesized because of liver damage from alcohol.

정상 성인에서 Haloperidol이 수면구조와 정신생리적 기능에 미치는 영향

윤미숙,신석철,왕성근 충남대학교 의과대학 지역사회의학연구소 1997 충남의대잡지 Vol.24 No.2

To investigate the effects of haolperidol on sleep structure and psychophysiologic function in normal adults, author applied polysomnography for sleep structure, reaction time for attention and visual analogue scales for daytime fatigue, mood and sleepiness to normal volunteers. The subjects were 8 healthy young adults, aged from 20 to 24 years old. The results were summarized as follows. 1. Haloperidol has the effects of decreased total sleep time, delayed sleep latency, increased slow wave sleep(SWS) and REM sleep, and decreased waking after sleep onset (WASO) and stage 1 sleep. 2. Delayed sleep latency continued until first recovery night, and increased REM sleep contiued nutil second recovery night. 3. In the night of haloperidol administration, decreased total sleep time correlated with increased slow wave sleep(SWS) and REM sleep, and increased daytime sleepiness, decreased waking after sleep onset(WASO) correlated with increased slow wave sleep(SWS) and REM sleep. Increased REM sleep correlated with decreased daytime sleepiness, and daytime fatigue correlated with mood. 4. In the first recovery night, decreased total sleep time correlted with increased REM sleep and continued until second recovery night, and daytime sleepiness correlated with fatigue, sleepiness and reaction time.

유산소운동이 고혈압환자의 혈청지질 및 호르몬에 미치는 영향

윤미숙 대한스포츠의학회 2000 대한스포츠의학회지 Vol.18 No.2

The purpose of this study was to investigate the changes of body composition, circulorespiratory functions, serum lipids and hormones related controlling blood pressure after 16weeks aerobic exrcise. Subjects were composed of twenty old female hypertensive patients. V˙O_2max was determined for each subjects by administering a treadmill exercise test. The exercise intensities are decided by regression equations between %HRmax and speed(m/min). Subjects were trained during 16weeks(50%HRmax, 4 frequencies/week, 1 hour). Paired t-test was used to evaluate the difference of the pre and post training. The 0.05 level of significance was as critical level for this study. The result of this study are as follows: 1. Weight and %fat were decreased after 16weeks aerobic training. 2. Circulorespiratory function was improved because of increasing in V˙O_2max, V˙O_2max/kg after 16weeks aerobic training. 3. As Total cholesterol, Low density lipoprotein cholesterol, Triglyceride and Apolipoprotein B were density and High density lipoprotein cholesterol was increased after 16weeks aerobic training, Blood pressure was decreased. 4. As plasma norepinephrine, renin, angiotension Ⅱ, Aldosterone and α-ANP wee decreased, so blood pressure was decreased because of decreasing total peripheral resistance In conclusion, Aerobic training was shown to positive influence on decreasing blood pressure because of improving plasma lipids and decreasing peripheral vascular resistance in hypertensive patients.

한국의 외식문화 변천에 관한 연구

尹淑賢,李媄貞 호남대학교 산업기술연구소 2003 산업기술연구논문집 Vol.11 No.-

테니스 포핸드시 밀어치기와 때려치기 타법의 운동학적 분석

윤양진,정미라,서국웅,이훈식,황명숙,강영택,윤길환 釜山大學校 附設 體育科學硏究所 1999 體育科學硏究所 論文集 Vol.15 No.-

This study consists in clarifying the meaning of various angles and velocity, and two types of the stroke of a pushing shot and hitting shot through the three-dimensional image analyses among the factors of exercise. To attain the purpose of such studies, the result of experiment and analysis for each of three athletes of boys and girls in high school and three persons in general came to the conclusion as follows; 1. The last motion of a group of A having a command of a follow shot showed greatly, compared with two groups of B and C, and this showed that a group of A took a rectilinear motion greatly to gain power and two group of B and C took a rotary motion greatly to gain speed. 2. A group of A having a command of a pushing shot move body weight forward from behind and give the first consideration to a rectilinear motion, and two groups of B and C having a command of the stroke of hitting shot used the turning of the shoulder and the waist and gave much weight to a rotary motion than a rectilinear one. 3. The position of the hitting point of a group of A having a command of a pushing shot turned out to be the waist, and that of the hitting point of two groups of B and C, the chest, and namely this showed that the position of the hitting point was decided according to the form of the stroke and pictures of various kinds. 4. In impacting, the speed decreased, for the power was controled to keep an accurate timing of stroke just before impacting

노루궁뎅이 버섯 분말을 첨가한 설기떡의 품질특성

윤숙자,이미영 한국조리과학회 2004 한국식품조리과학회지 Vol.20 No.6

This study was carried out to investigate the characteristics of Hericium erinaceus sulgidduk. The moisture content of Hericium erinaceus powder sulgidduk showed no significant difference between the added Hericium erinaceus powder and control groups. The L value decreased as the Hericium erinaceus powder concentration increased. The 'a' and 'b' color values were increased significantly when the amount of Hericium erinaceus powder was increased. The hardness, cohesiveness, chewiness and gumminess decreased with increasing Hericium erinaceus powder addition. From the sensory evaluation, Hericium erinaceus sulgidduk with the additions of 3 and 6% Hericium erinaceus powder were the most preferred with regard tochewiness, softness and overall acceptability. These results indicated that Hericium erinaceus sulgidduk with the addition of 3 and 6% Hericium erinaceus powder have the best quality.

학교 통일교육지침에 입각한 유치원의 통일교육 구현 방향 모색

윤기영,이미숙 서원대학교 교육연구소 1995 敎育發展 Vol.1995 No.14

The purpose of this study was to investigate how the kindergarten education for reunification in Korea should be, based on the 「Guides for School Teacher」published by Education Ministry. Necessity, appropriateness, and possibility of this education were considered. The results of this study were as follows; Education for reunification in Korea must be done in kindergarten as well as inordinary school because reunification of Korea is essential for us. Also many concepts and characters related with this education are formed and developed in early childhood. And it is one of reasons that early childhood deucation is very important as well as known. So, for successful this education systematic programs should be developed, and education about reunification should be reinforced for kindergarten teacher. Howerer, for the time being, since new programs are not yet developed it is desirable that the current programs such as 「human Education」, 「Democratic Citizenship Education」,「Education for peace」 be used instead to achieve this goal.

중량물 운반시 잡기유형과 부하단계에 따른 생리학적 연구

윤양진,이훈식,윤길환,정미라,이재규,이중숙,서국웅 釜山大學校 附設 體育科學硏究所 1999 體育科學硏究所 論文集 Vol.15 No.-

The purpose of this study was to examine physiological changes of the body according to various weight loads and the holding during carrying object. The experiments were conducted with utilizing physiological analyses for five male and five female adults as subjects. The results were as followed. 1. Any significant difference was not shown in heart rate, ventilation and amount of oxygen uptake of male subjects in the case of no load and 5kg. And also no significant difference was shown in expiratory exchange ratio of male subjects of no load, 5kg and 10kg, but in other measure items and other stages of weight was shown. 2. Significant differences were shown in heart rate, ventilation, defluxion of carbon dioxide, oxygen uptake per body weights, respiratory exchange rate, respiration rate and all other measure items of female subjects in all stages of load. 3. In the MMH job, there was no influence on male subjects up to 5kg loads, but there was an significant difference in male subjects in load walking from 5kg. It shows that even in 5kg weight MMH work, female subject's expiratory function was influenced. 4. 10kg load was the limit weight of female subjects and 15kg was of male subjects when they walked on treadmills with 4km/h velocity for 3 minutes, holding loads in the right and vertical types.

치과진료실에서의 감염방지 대책에 관한 조사

윤미숙,홍연표,정연강 중앙대학교 의과대학 간호학과 간호과학연구소 1997 중앙간호논문집 Vol.1 No.2

To investigate on infection control in some dental clinics in korea. This study was performed on 300 hygienists who are registered on the association of korea dental hygienist and work at dental clinics from November to December, 1995. Each subjects completed the questionnaire which compased of place of employment of dental hygienist, location of clinics, number of outpatients, educational experience about infection control of dental hygienist on infection control and age of dentists as independent varianbles and use of sterilization methods and protection devices as dependent variables. Data Was expressed as percentage and anlayzed as chi-square method using SPSS/PC + 4.0. There was a significant higher performance rate in use of sterilization methods and protection devices in dental clinics of general hospital than private and public dental clinics. There was no difference in use of sterilization methods and protection devices according to location, number of outpatients of dental clinics and age of dentists. In case of dental hygienists who getting educational experience about infection control, the rate of use of sterilization methods and protective devices was higher than no educational experience. This results suggested that the education about infection control and the follow-up study to investigate he causes of low performance in use sterilizsation methods and protective devices in dental clinics was needed.