한국인 직무 스트레스 측정도구의 개발 및 표준화

장세진,고상백,강동묵,김성아,강명근,이철갑,정진주,조정진,손미아,채창호,김정원,김정일,김형수,노상철,박재범,우종민,김수영,김정연,하미나,박정선,이경용,김형렬,공정옥,김인아,김정수,박준호,현숙정,손동국 大韓産業醫學會 2005 대한직업환경의학회지 Vol.17 No.4

Background and Purposes: Over the past three decades, numerous studies performed in Korea have reported that job stress is a determinant risk factor for chronic diseases and work disability. Every society has its own culture and occupational climate particular to their organizations, and hence experiences different occupational stress. An occupational stress measurement tool therefore needs to be developed to estimate it objectively. The purpose of this study is to develop and standardize the Korean Occupational Stress Scale (KOSS) which is considered to be unique and specific occupational stressors in Korean employees. Subjects and Methods: Data were obtained from the National Study for Development and Standardization of Occupational Stress (NSDSOS Project: 2002-2004). A total of 12,631 employees from a nationwide sample proportional to the Korean Standard Industrial Classification and the Korean Standard Occupational Classification were administered. The KOSS was developed for 2 years (2002-2004). In the first year, we collected 255 items from the most popular job stress measurement tools such as JCQ, ERI, NIOSH and OSI, and 44 items derived from the a qualitative study (depth interview). Forty-three items of KOSS, in the second year, were retained for use in the final version of the KOSS by using Delphi and factor analysis. Items were scored using conventional 1-2-3-4 Likert scores for the response categories. Results: We developed eight subscales by using factor analysis and validation process: physical environment (3 items), job demand (8 items), insufficient job control (5 items), interpersonal conflict (4 items), job insecurity (6 items), organizational system (7 items), lack of reward (6 items), and occupational climate (4 items). Together they explained 50.0% of total variance. Internal consistency alpha scores were ranged from 0.51 to 0.82. Twenty-four items of the short form of the KOSS (KOSS-SF) were also developed to estimate job stress in the work setting. Because the levels of the subscales of occupational stress were gender dependent, gender-specific standard norms for both the 43-item full version and the 24-item short form using a quartile for the subscales of KOSS were presented. Conclusion: The results of this study suggest that KOSS might be an appropriate measurement scale to estimate occupational stress of Korean employees. Further and more detailed study needs to be conducted to improve the validity of this scale.

기능성 Catechol-O-Methyltransferase 유전자 다형성과 한국인 정신분열병의 관련성

박태원,홍영준,박임순,김형섭,최인미,배수영,윤경식,강대희 大韓神經精神醫學會 2001 신경정신의학 Vol.40 No.4

연구목적 : Catechol-O-Methyltransferase(COMT)는 카테콜아민 신경전달물질의 불활성화를 담당하는 효소로서, 정신분열병과 관련된 후보 유전자로서 알려져 왔다. 최근, 기능성 COMT유전자 다형성과 정신분열병의 관련성이 제기되었는데, 한국인 정신분열병에서도 이러한 관련성이 있는지를 살피기 위해 본 연구를 시행하였다. 방 법 : 정신분열병으로 진단된 환자군 103명과 성별 및 연령별로 1:1 짝짓기 대조군 사이에 유전자형과 대립유전자의 분포를 비교하였다. 또한, 환자군은 공격적 행동, 가족력, 아동기 발병 여부에 따라 두 군으로 나누어 비교하였다. DNA의 다형성 부의를 중합효소연쇄반응에 의해 증폭한 후 ,NlaⅢ제한효소법과 전기영동을 실시하여 유전자형을 판별하였다. 결 과 : 환자군 전체와 대조군 사이에 유전자형과 대립유전자 빈도의 차이가 없었다. 가족력이 있는 환자군을 대조군과 비교하였을 때는, 대립유전자 L을 포함하는 유전자형과 그렇지 않은 유전자형의 분포에 있어 유의한 차이를 보였다(p=0.02, OR=3.9, 95% Cl=1.1∼14.3). 결 론 : 한국인 정신분열병과 기능성 COMT 유전자 다형성 사이에 유의한 관련성이 없었다. 따라서, 기능성 COMT 유전자 다형성이 한국인 정신분열병의 발병에 영향을 주지 못한다. 그러나, 정신분열병 가족력과 기능성 COMT유전자 다형성의 관련성이 시시돠어, 향후 본 연구에서 나타난 방법론적인 문제점을 보완하고 추가 대상자를 확보한 연구가 필요하다. Objectives : Catechol-O-methyltransferase(COMT) is involved in the degradation of catecholamine meurotransmitters and has been investigated as a candidate gene in schizophrenia. Recently, possible relationship between functional COMT gene polymorphism and schizophrenia has been suggested. To address the possible role of functional COMT gene polymorphism in the predisposition to schizophrenia, we carried out an association study in Korean schizophrenic patients and controls. Methods : One hundred and three Korean inpatients diagnosed as schizophrenia and 103 age and sex matched controls were selected as study subjects. Patients were subgrouped into two groups on the basis of history of aggressive behavior, family history of schizophrenia and related disorders, and age at onset. We determined COMT genotypes using PCR of the relevant region followed by digestion with NlaⅢ and electrophoresis. Results : No significant differences of allele and genotype frequencies were noted between patients and controls. However, when patients were categorized by the presence of family history of schizophrenia and related disorders, patients with family history showed almost 4 fold higher frequency of having COMT L allele containing genotype compared to controls(p=0.02, OR=3.9, 9.5% CI=1.10-14.33). Conclusion : Although our results do not support an association between functional polymorphism of COMT gene and schizophrenia overall, the findings suggest an association between functional COMT gene polymorphism and familial schizophrenia. Further studies with large samples are needed to confirm this association.

비효소적 핵산 분해 반응 : DNA에 대한 몇가지 Organometallointercalator Some Organometallointercalator for DNA

고동성,박미경,류형원,서일환 충남대학교부설 생명공학연구소 1991 생물공학연구지 Vol.1 No.-

Cu^(2+) -nalidixate 또는 Cu^(2+) -pipemidate 시스템을 H_2O_2 및 아스코르빈산 존재하 방사능-표지된 DNA와 함께 37℃에서 배양시키므로써 산-용해성 분해산물들이 생성되었다. 본성 DNA 대신 변성 DNA를 기질로 쓸 경우 금속-drug 시스템의 DNA 절단활성은 현저하게 감소되었다. 또한 본성 DNA에 대한 절단활성은 DNA에의 전형적 intercalator이며 metalloquinolones의 DNA에의 결합에 대한 경쟁적 방해제가 될 수 있는 ethidium bromide에 의하여 저하되었다. Quinolone 항생제가 금속이온과의 킬레이트 복합체 형성을 통하여 DNA의 두가닥 나사선 염기쌍 사이에의 삽간결합(intercalation)을 할 수 있으며 이와 같은 결합을 이룬 금속복합체는 DNA 가닥에 대한 산화성 절단활성을 가질 수 있음을 시사한다. Incubation of Cu^(2+)-nalidixate or Cu^(2+)-pipemidate system with DNA at 37℃ in the presence of H_2O_2 and ascorbic acid resulted in the production of acid-soluble counts from dadioactively labeled DNA. With denatured DNA as substrate, the cleavage activity of the metalloquinolone systems was far less than with the native DNA. The DNA cleavage activity of metalloquinolones was also decreased by the addition of ethidium bromide, which was known to be a typical intercalator of DNA and was shown to be a competitive inhibitor for the metalloquinolone binding to DNA. The data here suggest the DNA cleavage activity of quinolone antibiotics intercalated into DNA as metal chelate complexes.

새로운 Acetylcholinesterase inhibitors의 합성

최순규,김형민,조승환,최학기,박유미,이용균,정대일,김인식,한정태 동아대학교 부설 기초과학연구소 2004 基礎科學硏究論文集 Vol.21 No.1

노인성 치매의 일종인 alzheimer's disease의 효과적인 억제제를 합성하기 위해 우리는 분자 모델링에 의한 가장 적합한 물질인 m-[(N,N,N-trimethylammonio)phenyl]boronic acid를 합성하였다. 출발물질인 3-aminophenylboronic acid monohydrate의 경우 boronic acid의 작용기인 hydroxyl group을 protecting시킴으로써 반응의 안정성을 기하였다. Quarternary ammonium salt는 과량의 methyliodide와 염기 촉매인 potassium hydrogen carbonate를 사용하여 용매인 methanol에서 반응시켜 상당히 높은 수율을 얻을 수 있었다. 또한 과량의 methyliodide와 염기촉매인 potassium hydrogen carbonate를 사용하여 용매인 methanol에서 반응시킨 결과 boronic acid의 protection없이도 안정하게 반응이 진행되어짐을 확인할 수 있었다. In order to syntheisize a effective inhibotor for alzheimer's disease, we synthesized m-[(N,N,N-trimethylammonio)phenyl]boronic acid 4 which is designed by molecular modeling form. We protected the hydroxyl group of 3-aminophenylboronic acid monohydrate 1 with ethlyne glycol to remove the reactivity if hydroxyl group. To synthesize m-[(N,N,N-trimethylammonio)phenyl]boronic acid 4, we reacted 3-aminophenylboronic acid monohydrate 1 with ezcess methyl iodide and potassium hydrogen carbonate as a base-catalyst in methanol. but On executed reaction without protection on hydroxyl group, we found out the result that the hydroxyl group of boronic acid group at 3-aminophenylboronic acid monohydrate 1 didn't react with excess methyliodide. Synthesized m-[(N,N,N-trimethylammonio)phenyl]boronic acid 4 is in progress about biological tests as a plausible acetylcholinesterase inhibitor.

전격성 제1형 당뇨병 1예

노동현,김도형,김나래,박종선,이창헌,김미경,최영식 고신대학교의과대학 2007 고신대학교 의과대학 학술지 Vol.22 No.2

전격성 제1형 당뇨병은 특발성 당뇨병환자에서 임상 경과가 짧고 진단 당시 심각한 대사 합병증을 동반하면서 췌장 효소 수치가 높고 췌도 특이 자가항체가 음성인 새로운 아형으로 알려져 있으며, 일본과 일본 외에서의 유병률에는 차이가 있는 것으로 보인다. 아직 전격성 제1형 당뇨병의 발생 기전은 명확하지 않으며 유전적 감수성이 있는 개체에서 바이러스 감염 등의 환경적인 인자와의 연관성이 가능한 병태생리로 제시되고 있으며 이에 대한 추가적인 연구가 요구된다. 저자들은 케톤산증으로 내원하여 전격성 제1형 당뇨병을 진단받은 증례를 경험하였기에 문헌고찰과 함께 보고 하는 바이다. Some patients with idiopathic type 1 diabetes have a fulminant disorder characterized by the absence of insulitis and of diabetes-related antibodies, a remarkably abrupt onset and high serum pancreatic enzyme concentrations. This is referred to as fulminant type 1 diabetes. A 29-year-old man suffering from abdominal pain, nausea and vomiting consulted to our hospital. Laboratory findings revealed high blood glucose level and the evidence of diabetic ketoacidosis, but the serum HbA1c was normal nevertheless. The low level of plasma C-peptide indicated the loss of endogenous insulin secretion. The patient satisfied the criteria for the diagnosis of fulminant type 1 diabetes. After the patient has been treated with insulin, his symptoms were improved and abnormal laboratory data were normalized. We report this case with a review of the literature

신규간호사 배치후 교육시 프리셉터쉽 적용 사례연구

김창희,김혜숙,조혜성,이순옥,함형미,박미미 성인간호학회 2000 성인간호학회지 Vol.12 No.4

The purpose of this study was to examine the effect of preceptorship on On the Job Training of new nurses. The research design was a retrospective survey and one group pre-rost test design. The subjects were 49 entry nurses and 49 preceptors The data were collected from Feburary 1, 1998 to December 1, 1998. One questionnair applied in this study consisted of general characteristics, the responses of new entry nurses and their preceptors and their qualities. The other questionnair consisted of job satisfaction of new entry nurses and Preceptors and their competencies. The data were analyzed for frequencies, mean score, standard deviation, t-test, and ANOVA. The results of the study were as follows : 1) Preceptees's responses were that the nurses' affiliation of unit. cooperation of other nurses, and competencies of nursing skills improved after OJT applied preceptorship. 2) Preceptors's answers were that preceptorship had a positive effect on their continuing education. 3) While preceptees's job satisfaction scores were high in interaction, administration, and organization, they were low in autonomy and pay. 4) Pre-competency scores were the same as post-competency scores. 5) After preceptorship, preceptors responded that their nursing skill & communication competency increased, but their nursing administration competency decreased. 6) Finally, preceptorship's other effects were as follows: ① Checklists for preceptee education at the nursing unit were developed separately. ② It was proved that 'preceptor interview note' was effective on manage preceptorship. ③ We obtained duty orierlap periods, medical-surgical units-1 month. 5 ICU-2 months. operaction room-3 months ④ We prepared a preceptor program based on the study results.

Colossal Absorption of Molecules Inside Single Terahertz Nanoantennas

Park, Hyeong-Ryeol,Ahn, Kwang Jun,Han, Sanghoon,Bahk, Young-Mi,Park, Namkyoo,Kim, Dai-Sik American Chemical Society 2013 Nano letters Vol.13 No.4

<P>Molecules have extremely small absorption cross sections in the terahertz range even under resonant conditions, which severely limit their detectability, often requiring tens of milligrams. We demonstrate that nanoantennas tailored for the terahertz range resolves the small molecular cross section problem. The extremely asymmetric electromagnetic environment inside the slot antenna, which finds the electric field being enhanced by thousand times with the magnetic field changed little, forces the molecular cross section to be enhanced by >10<SUP>3</SUP> accompanied by a colossal absorption coefficient of ∼170 000 cm<SUP>–1</SUP>. Tens of nanograms of small molecules such as 1,3,5-trinitroperhydro-1,3,5-triazine (RDX) and lactose drop-cast over an area of 10 mm<SUP>2</SUP>, with only tens of femtograms of molecules inside the single nanoslot, can readily be detected. Our work enables terahertz sensing of chemical and biological molecules in ultrasmall quantities.</P><P><B>Graphic Abstract</B> <IMG SRC='http://pubs.acs.org/appl/literatum/publisher/achs/journals/content/nalefd/2013/nalefd.2013.13.issue-4/nl400374z/production/images/medium/nl-2013-00374z_0001.gif'></P><P><A href='http://pubs.acs.org/doi/suppl/10.1021/nl400374z'>ACS Electronic Supporting Info</A></P>

Functional characterization of the SIZ/PIAS-type SUMO E3 ligases, OsSIZ1 and OsSIZ2 in rice

PARK, HYEONG CHEOL,KIM, HUN,KOO, SUNG CHEOL,PARK, HEE JIN,CHEONG, MI SUN,HONG, HYEWON,BAEK, DONGWON,CHUNG, WOO SIK,KIM, DOH HOON,BRESSAN, RAY A.,LEE, SANG YEOL,BOHNERT, HANS J.,YUN, DAE-JIN Blackwell Publishing Ltd 2010 Plant, cell and environment Vol.33 No.11

<P>ABSTRACT</P><P>Sumoylation is a post-translational regulatory process in diverse cellular processes in eukaryotes, involving conjugation/deconjugation of small ubiquitin-like modifier (SUMO) proteins to other proteins thus modifying their function. The PIAS [protein inhibitor of activated signal transducers and activators of transcription (STAT)] and SAP (scaffold attachment factor A/B/acinus/PIAS)/MIZ (SIZ) proteins exhibit SUMO E3-ligase activity that facilitates the conjugation of SUMO proteins to target substrates. Here, we report the isolation and molecular characterization of <I>Oryza sativa</I> SIZ1 (OsSIZ1) and SIZ2 (OsSIZ2), rice homologs of <I>Arabidopsis</I> SIZ1. The rice SIZ proteins are localized to the nucleus and showed sumoylation activities in a tobacco system. Our analysis showed increased amounts of SUMO conjugates associated with environmental stresses such as high and low temperature, NaCl and abscisic acid (ABA) in rice plants. The expression of <I>OsSIZ1</I> and <I>OsSIZ2</I> in <I>siz1-2 Arabidopsis</I> plants partially complemented the morphological mutant phenotype and enhanced levels of SUMO conjugates under heat shock conditions. In addition, ABA-hypersensitivity of <I>siz1-2</I> seed germination was partially suppressed by <I>OsSIZ1</I> and <I>OsSIZ2.</I> The results suggest that rice SIZ1 and SIZ2 are able to functionally complement <I>Arabidopsis</I> SIZ1 in the SUMO conjugation pathway. Their effects on the <I>Arabidopsis</I> mutant suggest a function for these genes related to stress responses and stress adaptation.</P>

Terahertz pinch harmonics enabled by single nano rods

Park, Hyeong-Ryeol,Bahk, Young-Mi,Choe, Jong Ho,Han, Sanghoon,Choi, Seong Soo,Ahn, Kwang Jun,Park, Namkyoo,Park, Q-Han,Kim, Dai-Sik The Optical Society 2011 Optics express Vol.19 No.24

Introduction of domestic laboratory animal resources (Korl) and fostering plan

Mi-Hyeong Park,Huibae Kim,Yeon Jeong Shin,Wonseob Kim,Gyeong Won Im,Geunhyeong Sakong,Young Chai Park,Hyun-Young Cho,Hyeona Ki,Heejung Shin,Somi Yun,Su-hae Lee,Jun Gyou Kim 한국실험동물학회 2020 한국실험동물학회 학술발표대회 논문집 Vol.2020 No.7