스타치의 숙성에 따른 이화학적 성분 변화

임상빈,좌미경 제주대학교 공과대학 첨단기술연구소 2002 尖端技術硏究所論文集 Vol.13 No.1

Field-and house-cultured Citrus sudachi were picked in two week's interval from September, and physicochemical properties were measured. Average weight, and ratios of flesh and juice increased, while ratio of peel decreased with the ripening of sudachi. Average weight of house-cultured sudachi was 25.6∼41.5g and that of field-cultured sudachi was 16.9∼29.1g. pH of juice from sudachi increased slightly, and total acidity increased greatly at the beginning of ripening and then decreased Soluble solid (°Brix) increased sharply at the beginning of ripening and stayed at the same level. Total acidity was low and soluble solid was high in the juice of house-cultured sudachi compared with that of field-cultured sudachi. Pectinesterase activity of the juice was decreased greatly and turbidity was increased with the ripening. The juice showed green and yellow color. The citric and malic acids were the major organic acids, while the ascorbic, oxalic and fumaric acids were the minor in the juice of sudachi. Oxalic, malic and citric acid were high, and ascorbic and fumaric acids were low in field-cultured sudachi than in house-cultures sudachi.

캡슐소재에 따른 멸치어유의 미세캡슐화

임상빈,좌미경,송대진 濟州大學校 産業技術硏究所 1999 산업기술연구소논문집 Vol.10 No.1

Microencapsulation of anchovy oil with different wall materials such as sodium alginate. chitosan and β-cyclodextrin was investigated. Microencapsulation was accomplished by ejecting an oil/water emulsion into a dispersion fluid under high pressure through an orifice. As a dispersion fluid, 0.2% calcium lactate was appropriate for sodium alginate, while 0.18 sodium hydroxide for chitosan in terms of capsule size and distribution, and emulsion stability. β -cyclodextrin formed inclusion complex with anchovy oil. The microcapsules were stored at 22 and 30℃ for 8 days, and their stability was tested. The acid value remained unchanged and the peroxide value was not detected.

초피 추출물의 수율 및 항균활성

임상빈,좌미경,송대진,고정삼 濟州大學校 産業技術硏究所 1995 산업기술연구소논문집 Vol.6 No.-

Extraction yields of Zunthoxylum piperiturn by organic solvents and supercritical carbon dioxide(SC-CO_(2)) with and without ethanol as a cosolvent, and antimicrobial activities of the extracts against Hucillus cereus were determined. Extraction yield by distilled water was the highest, 20%, while those by ethanol, ethyl ether and hexane were less than 3%. Extraction yield by SC-CO_(2) Lvith ethanol was 1.42 times greater than that by only SC-CO_(2). Ethyl ether- and ethanol-extracts had potent antimicrobial activities against B. cereus. Extraction yield by SC-CO_(2) with ethanol showed almost the same degree of microbial growth inhibition as that by only SC-CO_(2).

당유자와 하귤의 숙성에 따른 이화학적 성분 변화

임상빈,좌미경 제주대학교 생명과학기술혁신센터 2002 제주생명과학연구 Vol.5 No.-

Dangyooja and Hageul were picked in two week’s interval from September, and physicochemical properties were measured. Average weight, and ratios of flesh and juice increased, while ratio of peel decreased with the ripening of Dangyooja and Hageul. Average weight of Dangyooja was higher than that of Hageul. Ratios of the juice from Dangyooja increased greatly compared with that of Hageul with the ripening. Total acidity of the juice from Dangyooja increased upto 3.61% and then decreased, and soluble solid was 7.9˚Brix. Total acidity and soluble solid of the juice from Hageul showed the same trend as that of Dangyooja except the higher values. Pectinesterase activity of the juice from Hageul was 1.76 times lower than that from Dangyooja. The juice from Hageul was more turbid than that from Dangyooja and showed green and yellow color. The citric and malic acids were the major organic acids, while the ascrbic, oxalic and fumaric acids were the minor in the juice. Citric and malic acids increased greatly and then decreased slightly with the ripening. Total organic acids of the juice from Dangyooja and Hageul were 3.25∼5.51% and 4.35∼6.04%, respectively

제주산 잡곡류를 이용한 미숫가루의 제조방법 개발

임상빈,좌미경,송대진 제주대학교 생명과학연구소 2003 제주생명과학연구 Vol.6 No.1

Cereal powder was processed using Jeju specialty grain and legumes such as milled upland glutinous rice, SoRiTae, JwiNunIKong and black sesame. Glutinous rice was soaked in water for 45 min at room temperature, steam-cooked for 10 min, dried for 45 min, roasted, and finally macerated without hull. SoRiTae and JwiNunIKong were roasted and macerated with hull without water-soaking and steam-cooking. The maceration of mixed grain and legumes for 10 min reduced the moisture content by less than 10%. Crude protein content was high in the untreated JwiNunIKong by 39.0%, and was increased slightly with steam-cooking and roasting. Crude ash content was decreased with maceration, and was high in macerated SoRiTae and JwiNunIKong with hull. The brightness was high in steam-cooked and roasted glutinous rice, and low in steam-cooked legumes. Water solubility index was high in steam-cooked glutinous rice, and decreased slightly in steam-cooked and roasted legumes. α-Degree was higher in the steam-cooked than the untreated, and was high in the order of JwiNunIKong, SoRiTae and glutinous rice. Sensory evaluation showed that the dispersion stability of cereal powder increased as the mixing ratio of glutinous rice decreased. Great preference on odor and overall acceptance were shown as the mixing ratio of glutinous rice decreased and that of legumes increased. Taste was correlated with the dispersion stability. The addition of sugar increased the solubility of powder and had no effect on dispersion stability.

가변 광도 중합에 따른 복합레진의 중합수축에 관한 연구

임미영,조경모,홍찬의 대한치과보존학회 2007 Restorative Dentistry & Endodontics Vol.32 No.1

본 연구는 광중합형 복합레진 중합 시 기존의 중합법과 가변광도 중합형인 soft start 중합법 및 exponential 중합법 간의 중합수축효과를 비교하고자 하였다. 본 연구를 위해 3종의 복합레진 (Synergy Duo Shade, Z250, Filtek Supreme) 및 3종의 광조사기 (Spectrum 800, Elipar Highlight, Elipar Trilight)를 사용하였다. 총 중합시간은 40초로 일정하게 유지하였으며, 선형 중합수축률의 측정은 linometer를 이용하였으며, 90초간의 선형 수축률을 0.5초 간격으로 측정하였다. 재료별로 각 중합 방법별 시간에 따른 중합수축률을 one-way ANOVA test로 분석하여 최종 중합수축률에 도달하는 시간을 산출하였고, 90초 후 최종 선형 중합수축률을 two-way ANOVA test를 이용하여 재료, 광조사 방법, 재료 및 광조사 방법의 교호작용이 중합수축에 미치는 영향이 있는지를 검증하였다. 또한 90초까지의 선형 중합수축률에 대한 20초까지의 선형 중합수축률의 비를 two-way ANOVA로 비교하고, 각각의 통계치를 95% Scheffe test로 검증하였는바, 다음과 같은 결과를 얻었다. 1. Supreme을 제외한 다른 군에서는 재료 및 광조사 방법에 관계없이 대부분의 중합 수축이 광조사 후 20초 이내에 이루어졌다 (p < 0.05). 2. 90초 후, 최종 중합수축률은 재료 (p = 0.000)와 광조사 방법 (p = 0.003) 모두 유의성 있는 영향을 끼쳤으나, 재료와 광조사 방법 상호간의 작용은 영향이 없었다. 3. 90초 후 최종 중합수축률은 총 광에너지가 가장 낮은 exponential 중합법에서 유의성 있게 낮았다 (p < 0.05). 4. 20초까지의 초기 수축률은 soft start와 exponential 중합법 등 가변광도 중합이 conventional 중합법에 비하여 유의성 있게 낮은 수축률을 보였다 (p < 0.05). 본 실험 결과만을 토대로 볼 때, 가변광도 중합법은 초기 중합수축 속도를 감소시켜 수축응력을 감소시킨다고 볼 수 있다. 그러나 총 조사 광에너지의 차이로 인해 그 물리적 성질에 영향이 있을 수 있으므로 향후 이에 대한 더 많은 고찰이 필요할 것으로 사료된다. The purpose of this study was to compare the effect of exponential curing method with conventional curing and soft start curing method on polymerization shrinkage of composite resins. Three brands of composite resins (Synergy Duo Shade, Z250, Filtek Supreme) and three brands of light curing units (Spectrum 800, Elipar Highlight, Elipar Trilight) were used. 40 seconds curing time was given. The shrinkage was measured using linometer for 90 seconds. The effect of time on polymerization shrinkage was analysed by one-way ANOVA and the effect of curing modes and materials on polymerization shrinkage at the time of 90s were analysed by two-way ANOVA. The shrinkage ratios at the time of 20s to 90s were taken and analysed the same way. The results were as follows: 1. All the groups except Supreme shrank almost within 20s. Supreme cured by soft start and exponential curing had no further shrinkage after 30s (p < 0.05). 2. Statistical analysis revealed that polymerization shrinkage varied among materials (p = 0.000) and curing modes (p = 0.003). There was no significant interaction between material and curing mode. 3. The groups cured by exponential curing showed the statistically lower polymerization shrinkage at 90s than the groups cured by conventional curing and soft start curing (p < 0.05). 4. The initial shrinkage ratios of soft start and exponential curing were statistically lower than conventional curing (p < 0.05). From this study, the use of low initial light intensities may reduce the polymerization rate and, as a result, reduce the stress of polymerization shrinkage.

초피 추출물의 수율 및 항균활성

임상빈,송대진,좌미경,고정삼 濟州大學校工科大學産業技術硏究所 1995 尖端技術硏究所論文集 Vol.6 No.-

초피를 유기용매 및 초임계이산화탄소와 보조용매로서 에탄올로 추출하여 각각의 추출수율을 측정하고, 이들 추출물들의 항균활성을 검색하였다. 유기용매별로 추출하여 각각의 추출수율을 측정한 결과, 증류수인 경우 20%로서 가장 높았고, 나머지 헥산, 에테르, 에탄올인 경우 3%이하로 매우 적었다. 또한 추출 용매로 초임계이산화탄소-에탄올을 병용했을 때 초피의 추출수율과 용해도는 초임계이산화탄소만을 사용했을 때보다 1.4배 이상 높았다. 용매별 추출물들의 항균성을 검색한 결과 모든 추출물들이 B.cereus에 대해 항균성을 나타내었는데, 특히 에탄올, 에테르 추출물들의 항균성이 더 큰 것으로 나타났다. 초임계이산화탄소-에탄올 추출물은 초임계이산화탄소만을 사용한 추출물과 거의 동일한 항균성을 나타내었다. Extraction yields of Zanthoxylum piperitum by organic solvents and supercritical carbon dioxide(SC-CO₂) with and without ethanol as a cosolvent, and antimicrobial activities of the extracts against Bacillus cereus were determined. Extraction yield by distilled water was the highest, 20%, while those by ethanol, ethyl ether and hexane were less than 3%. Extraction yield by SC-CO₂ with ethanol was 1.42 times greater than that by only SC-CO₂ Ethyl ether- and ethanol-extracts had potent antimicrobial activities against B.cereus. Extraction yield by SC-CO₂ with ethanol showed almost the same degree of microbial growth inhibition as that by only SC-CO₂.

The Relationship between the Salivary IgA against Ag I/II of S.mutans and Dental Caries Experience among Children and Adults

임수민,김재곤,백병주,양연미,김수경,이경열 大韓小兒齒科學會 2008 大韓小兒齒科學會誌 Vol.35 No.4

치아 우식증은 감염성 질환의 하나로 치아우식의 원인균은 Streptococcus mutans(S. mutansJ와 같은 mutans stepto-cocci로 알려져 있다. S. mutans가 치면에 접착하여 군집을 형성하는 능력은 균독성에 중요한 역할을 하는데. Agl/IT와 같은 세포 표면의 섬유성 단백질을 매개로 한다. Secretory 1gA는 타액이나 누 · 비액, 초유, 그리고 폐나 소화기관의 분비액에서 선택적으로 다량 발견되는데 타액에서 se cretory 19A는 S. mutans의 대사활동을 억제하고 치면으로의 부착을 방해한다. 이전의 몇몇 연구에서 S. mutans에 특이적인 타액 내 IgA와 우식경험도는 역상관관계를 보인다고 발표하였다. 그러나 다른 연구에서 통계적 유의성이 없다고 보고하기도 하였다. 본 연구의 목적은 소아 성인의 치아우식증과 S. mutans의 Ag I/II에 특이적인 타액 내 IgA와의 관계를 알가위한 것이다. 이를 위해 소아(평균6세) 28명, 성인(20-30세) 18명을 대상으로 Group I(deft index ≤ 3). Group II (deft index ≥ 4), Group III (DMFT index ≤ 3), Group IV (DMFT index ≥ 4)로 분류하였다. 그리고 caries resistant group(CR group, deft or DMFT index ≤ 3)과 caries susceptible group(CS group. deft or DMFT index ≥ 4)으로 분류하였다. S. mutans 수와 우식 경험도 간에는 통계적으로 유의 한 상관관계를 나타냈다. Agl/II 특이 salivary IgA titer는 Group III 이 Group II 보다 통계적으로 유의하게 더 컸으며, CR group이 CS group보다 유의하게 크게 나타났다. AgI/II of Streptococcus muiens(S. mutens) is an important virulence factor that contributes to the pathogenesis of S. mutans-induced dental caries. In oral cavity, salivary IgA antibodies act as safeguards against enormous challenges from oral bacteria. IgA antibodies inhibit adherence of cariogenic microorganisms to hard surfaces. Analysis of salivary IgA against AgI/II can be very useful diagnostic and powerful communication tools to the dental caries The purpose of this study was to investigate correlation between salivary AgI/II specific IgA and incidence of dental caries among children and young adults. Subjects consisted of 28 children and 18 adults. They were as￢signed to four groups: Group I (deft index ≤ 3), Group lI (deft index ≥ 4), Group Ill (DMFT index ≤ 3), Group IV (DMFT index ≥ 4) and they was divided two groups into caries resistant group and caries susceptible group. The study group were examined caries activity and their salivary IgA was evaluated by enzyme-linked immunosorbent assay. The results are as follows : 1. There was a positive correlation between the number of S. mutans and caries activity. 2.The titer of salivary IgA against the AgI/II was significantly higher in caries resistant group than caries susceptible group(p<0.01). 3. The titer of salivary IgA against the AgI/II in Group Ill was significantly higher than Group II (p<0.05).

초임계이산화탄소에 의한 유기염소계 잔류농약의 포집효율

임상빈,좌미경,김수현 濟州大學校 産業技術硏究所 1996 산업기술연구소논문집 Vol.7 No.1

Collection efficiencies of organochlorine pesticides by supercritical fluid extraction(SFE) was investigated to develop a simple, fast and inexpensive extraction method. Standard organochlorine pesticides mixture(SOPM, no water) showed no evidence of metering valve plugging in contrast to the plugging associated with SFE of fish tissues(wet sample). Maximum percent recovery of SOPM was obtained at bar by collecting the extract within 3 min through the metering valve without heating into a capped screw-top tube half filled with solid CO_(2) Recovery of SOPM itself shwes 98.3% while recovery of that spiker in fish tissue 85.6%.

건조 방식에 따른 선과 공정별 감귤의 품질 변화

임상빈,좌미경,정성근 제주대학교 공과대학 첨단기술연구소 2003 尖端技術硏究所論文集 Vol.14 No.1

Effects of drying methods such as flame-air and hot water-air after waxing of fruits in a packinghouse line on quality of Citrus unshiu were investigated in terms of acidity, pH, soluble solid, vitamin C, and ratios of flesh, peel and juice. Flame-air drying system played a major role in increasing room and fruit temperature in all of the line processes. Acidities of Citrus juice decreased greatly with the line processes in flame-air drying system compared with those in hot water-air circulation system. Not much changes in soluble solid contents were found. Degradation of vitamin C was accelerated in flame-air drying system. Ratio of flesh decreased, while that of peel increased with the line processes in flame-air drying system. Ratio of juice increased with the line processes in hot water-air circulation system.