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( Kianoush Khajeh Reshidan ),( Nasha Nassoury ),( Abderrazzak Merzouki ),( Claude Guertin ) 생화학분자생물학회 2002 BMB Reports Vol.35 No.6
A gene that encodes a protein homologue to baculoviral IE-1 was identified and sequenced in the genome of the Choristoneura fumiferana granulovirus (ChfuGV). The gene has an 1278 nucleotide (nt) open-reading frame (ORF) that encodes 426 amino acids with an estimated molecular weight of 50.33 kDa. At the nucleotide level, several cis-acting regulatory elements were detected within the promoter region of the ie-1 gene of ChfuGV along with other studied granuloviruses (GVs). Two putative CCAAT elements were detected within the noncoding leader region of this gene; one was located on the opposite strand at -92 and the other at -420 nt from the putative start triplet. Two baculoviral late promoter motifs (TAAG) were also detected within the promoter region of the ie-1 gene of ChfuGV. A single polyadenylation signal, AATAAA, was located 18nt downstream of the putative translational stop codon of ie-1 from ChfuGV. At the protein level, the amino acid sequence data that was derived from the nucleotide sequence in ChfuGV IE-1 was compared to those of the Cydia pomonella granulovirus (CpGV), Xestia c-nigrum granulovirus (XcGV) and Plutella xylosteila granulovirus (PxGV). The C-terminal regions of the granuloviral IE-1 sequences appeared to be more conserved when compared to the N-terminal regions. A domain, similar to the basic helix-loop-helix like (bHLH-like) domain in NPVs, was detected at the C-terminal region of IE-1 from ChfuGV (residues 387 to 414). A phylogenetic tree for baculoviral IE-1 was constructed using a maximum parsimony analysis, A phylogenetic estimation demonstrates that ChfuGV IE-1 is most closely related to that of CpGV.
Rashidan, Kianoush Khajeh,Nassoury, Nasha,Merzouki, Abderrazzak,Guertin, Claude 생화학분자생물학회 2002 Journal of biochemistry and molecular biology Vol.35 No.6
A gene that encodes a protein homologue to baculoviral IE-1 was identified and sequenced in the genome of the Choristoneura fumiferana granulovirus (ChfuGV). The gene has an 1278 nucleotide (nt) open-reading frame (ORF) that encodes 426 amino acids with an estimated molecular weight of 50.33 kDa. At the nucleotide level, several cis-acting regulatory elements were detected within the promoter region of the ie-1 gene of ChfuGV along with other studied granuloviruses (GVs). Two putative CCAAT elements were detected within the noncoding leader region of this gene; one was located on the opposite strand at -92 and the other at -420 nt from the putative start triplet. Two baculoviral late promoter motifs (TAAG) were also detected within the promoter region of the ie-1 gene of ChfuGV. A single polyadenylation signal, AATAAA, was located 18nt downstream of the putative translational stop codon of ie-1 from ChfuGV. At the protein level, the amino acid sequence data that was derived from the nucleotide sequence in ChfuGV IE-1 was compared to those of the Cydia pomonella granulovirus (CpGV), Xestia c-nigrum granulovirus (XcGV) and Plutella xylostella granulovirus (PxGV). The C-terminal regions of the granuloviral IE-1 sequences appeared to be more conserved when compared to the N-terminal regions. A domain, similar to the basic helix-loop-helix like (bHLH-like) domain in NPVs, was detected at the C-terminal region of IE-1 from ChfuGV (residues 387 to 414). A phylogenetic tree for baculoviral IE-1 was constructed using a maximum parsimony analysis. A phylogenetic estimation demonstrates that ChfuGV IE-1 is most closely related to that of CpGV.
Abderraouf Messai,Lahcene Fortas,Tarek Merzouki,Mohammed Sid Ahmed Houari 국제구조공학회 2022 Structural Engineering and Mechanics, An Int'l Jou Vol.81 No.4
A finite element method analysis framework is introduced for the free vibration analyses of functionally graded porous beam structures by employing two variables trigonometric shear deformation theory. Both Young’s modulus and material density of the FGP beam element are simultaneously considered as grading through the thickness of the beam. The finite element approach is developed using a nonlocal strain gradient theory. The governing equations derived here are solved introducing a 3-nodes beam element. A comprehensive parametric study is carried out, with a particular focus on the effects of various structural parameters such as the dispersion patterns of GPL reinforcements and porosity, thickness ratio, boundary conditions, nonlocal scale parameter and strain gradient parameters. The results indicate that porosity distribution and GPL pattern have significant effects on the response of the nanocomposite beams.
Lahcene Fortas,Abderraouf Messai,Tarek Merzouki,Mohammed Sid Ahmed Houari 국제구조공학회 2022 Steel and Composite Structures, An International J Vol.43 No.1
This paper is concerned with the buckling behavior of functionally graded graphene reinforced porous nanocomposite beams based on the finite element method (FEM) using two variables trigonometric shear deformation theory. Both Young’s modulus and material density of the FGP beam element are simultaneously considered as grading through the thickness of the beam. The finite element approach is developed using a nonlocal strain gradient theory. The governing equations derived here are solved introducing a 3-nodes beam element, and then the critical buckling load is calculated with different porosity distributions and GPL dispersion patterns. After a convergence and validation study to verify the accuracy of the present model, a comprehensive parametric study is carried out, with a particular focus on the effects of weight fraction, distribution pattern of GPL reinforcements on the Buckling behavior of the nanocomposite beam. The effects of various structural parameters such as the dispersion patterns for the graphene and porosity, thickness ratio, boundary conditions, and nonlocal and strain gradient parameters are brought out. The results indicate that porosity distribution and GPL pattern have significant effects on the response of the nanocomposite beams, and the results allows to identify the most effective way to achieve improved buckling behavior of the porous nanocomposite beam.
Shear correction factors of a new exponential functionally graded porous beams
Mohammed Sid Ahmed Houari,Aicha Bessaim,Tarek Merzouki,Ahmed Amine Daikh,Aman Garg,Abdelouahed Tounsi,Mohamed A. Eltaher,Mohamed-Ouejdi Belarbi 국제구조공학회 2024 Structural Engineering and Mechanics, An Int'l Jou Vol.89 No.1
This article introduces a novel analytical model for examining the impact of porosity on shear correction factors (SCFs) in functionally graded porous beams (FGPB). The study employs uneven and logarithmic-uneven modified porositydependent power-law functions, which are distributed throughout the thickness of the FGP beams. Additionally, a modified exponential-power law function is used to estimate the effective mechanical properties of functionally graded porous beams. The correction factor plays a crucial role in this analysis as it appears as a coefficient in the expression for the transverse shear stress resultant. It compensates for the assumption that the shear strain is uniform across the depth of the cross-section. By applying the energy equivalence principle, a general expression for static SCFs in FGPBs is derived. The resulting expression aligns with the findings obtained from Reissner’s analysis, particularly when transitioning from the two-dimensional case (plate) to the onedimensional case (beam). The article presents a convenient algebraic form of the solution and provides new case studies to demonstrate the practicality of the proposed formulation. Numerical results are also presented to illustrate the influence of porosity distribution on SCFs for different types of FGPBs. Furthermore, the article validates the numerical consistency of the mechanical property changes in FG beams without porosity and the SCF by comparing them with available results.
On static bending of multilayered carbon nanotube-reinforced composite plates
Ahmed Amine Daikh,Ismail Bensaid,Attia Bachiri,Mohamed Sid Ahmed Houari,Abdelouahed Tounsi,Tarek Merzouki 사단법인 한국계산역학회 2020 Computers and Concrete, An International Journal Vol.26 No.2
In this paper, the bending behavior of single-walled carbon nanotube-reinforced composite (CNTRC) laminated plates is studied using various shear deformation plate theories. Several types of reinforcement material distributions, a uniform distribution (UD) and three functionally graded distributions (FG), are inspected. A generalized higher-order deformation plate theory is utilized to derive the field equations of the CNTRC laminated plates where an analytical technique based on Navier’s series is utilized to solve the static problem for simply-supported boundary conditions. A detailed numerical analysis is carried out to examine the influence of carbon nanotube volume fraction, laminated composite structure, side-to-thickness, and aspect ratios on stresses and deflection of the CNTRC laminated plates.