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박상수(Sang-Soo Park),심미령(Mi-Ryung Sim),이충기(Chung-Ki Lee) 한국경제연구학회 2017 한국경제연구 Vol.35 No.3
2007년부터 시행되고 있는 한국의 주택연금제도는 정부가 보증하는 역모기지 프로그램이다. 한국주택금융공사가 매년 실시하고 있는 연간 설문 자료를 이용하여 미래에 주택연금을 이용할 의향이 있는 가구들에 대해 주택연금에 대한 수요를 추정하였다. 본 논문에서는 이충기․박상수(2014)의 연구를 확장하여 미래 기대와 코호트 효과를 고려하여 순서형 로짓 모형을 설정하였다. 설문에서 진술된 가구의 의향이 미래에 대한 기대에 근거하고 있다는 점을 적절하게 평가하기 위해 몇 가지의 가정을 바탕으로 미래의 기대형성 과정을 계량경제학적으로 모형화하여 주택연금 수요를 예측하고자 하였다. 주택연금에 대한 수요 추정 결과에 따르면, 주택연금 프로그램에 대해 후세대들이 보다 덜 긍정적인 경향이 나타난 반면 저소득 계층은 보다 긍정적으로 나타났다. The J ooTaekYeonKeum is the government guaranteed reverse mortgage program that Korean Government has been implementing since 2007. We extended Lee and Park’s(2014) demand estimation for the reverse mortgage loans in Korea using the yearly survey data, conducted by Korea Housing Financing Corporation, for household’s intention to use J ooTaekYeonKeum in the future. In order to appropriately appreciate the fact that the stated intentions in the survey were based on households’ expectations for their future, we adopted an econometric procedure that incorporated expectations explicitly as well as cohort effects. Our findings include: demands for reverse mortgage loans are estimated similar to that of Lee and Park(2014); later generations tend to be less positive for having the reverse mortgage program; lower income groups are more positive for the program.
Developmental Competence and Targeting Efficiency of CRISPR/Cas9 Mediated hG-CSF
Mi-Ryung Park,Ji-Hyun Lim,Kyong-Woon Kim,Hak-Jae Chung,Poongyeon Lee,Taiyoung Hur,Gi-Sun Im 한국수정란이식학회 2016 한국수정란이식학회 학술대회 Vol.2016 No.10
The clustered regularly interspaced short plalindromic repeats(CRISPR)/CRISPR associated protein (Cas9) system can be applied to produce transgenic pigs. We applied CRISPR/Cas9 system to generate hG-CSF targeted pig parthenogenetic embryos. Using sigle guided RNA targeted to pig hG-CSF genes was injected into cytoplasm of in vitro matured oocyte before electrical activation. The CRISPR/Cas9 vector were diluted in Tris-EDTA buffer (TE buffer) and injected with different concentration of 0 (sham injection), 2.5 and 25 ng/ul. In results, regardless of the concentrations of vector, the cleavage and blastocyst rate were not significantly different among three groups. Since plasmid DNA was used for microinjection, we investigated whether DNA vectors were integrated into the genome. Genomic PCR of the coding sequence of Cas9 variants and hG-CSF was performed to detect genomic integrants. Each blastocysts were collected into a microtube, and then PCR was performed. Overall 32 embryos are not expressed targeted gene.
Chromosome remodeling and differentiation of tetraploid embryos during preimplantation development
Park, Mi‐,Ryung,Lee, Ah‐,Reum,Bui, Hong‐,Thuy,Park, Chankyu,Park, Keun‐,Kyu,Cho, Ssang‐,Goo,Song, Hyuk,Kim, Jae‐,Hwan,Van Thuan, Nguyen,Kim, Jin‐,Hoi Wiley‐Liss, Inc. 2011 Developmental dynamics Vol.240 No.7
<P><B>Abstract</B></P><P>Although it is known that the tetraploid embryo contributes only to the placenta, the question of why tetraploid embryos differentiate into placenta remains unclear. To study the effect of electrofusion on the development of mouse tetraploid oocytes, mouse two‐cell embryos were fused and cultured in vitro in Chatot‐Ziomek‐Bavister medium. After electrofusion, two chromosome sets from the tetraploid blastomere were individually duplicated before nuclear fusion. At 8–10 hr after electrofusion, each chromosome set was condensing and the nuclear membrane was breaking down. Around 12–14 hr after electrofusion, the two chromosome sets had combined together and had reached the second mitotic metaphase, at this point with 8n sets of chromosomes. Interestingly, we discovered that expression of OCT4, an inner cell mass cells biomarker, is lost by the tetraploid expanded blastocysts, but that CDX2, a trophectoderm cells biomarker, is strongly expressed at this stage. This observation provides evidence clarifying why tetraploid embryos contribute only to trophectoderm. Developmental Dynamics 240:1660–1669, 2011. © 2011 Wiley‐Liss, Inc.</P>
Production of Transgenic Male Piglet as Research Model for Alzheimer's Disease
Mi-Ryung Park,Kyong-Woon Kim,Jae-Seok Woo,Seongsoo Hwang,In-Sul Hwang1,Tae-Uk Kwak,Ji-Hyun Lim,Se-Pil Park 한국동물생명공학회(구 한국동물번식학회) 2017 발생공학 국제심포지엄 및 학술대회 Vol.2017 No.10
Alzheimer’s disease (AD) is a progressive neurodegenerative disease associated with memory loss and cognitive impairments. An AD transgenic (Tg) pig model would be useful for preclinical testing of therapeutic agents. In this study, we report the use of somatic cell nuclear transfer (SCNT) to produce transgenic pigs over-expressing the human AD related genes (APP, APPswedish, Presenilin 1, and Tau). Transgenic embryos were generated by SCNT of from ear fibroblasts expressing AD genes. A total of 1808 (average 258) SCNT embryos were transferred into 7 recipients. Pregnancy was successfully maintained in one recipient. We obtained 1 cloned male piglet from a surrogate gilts and the weight of piglet was 935 g. But, the male piglet died two days after birth. The piglet expressed AD related genes by PCR and western blot analysis. Transgenes were expressed in multiple tissues, and at especially high levels in brain. AD Tg pig might be very useful for studying the disease and for testing new therapeutics in preclinical studies of human AD.
Production of Human TPA Transgenic Pigs by Somatic Cell Nuclear Transfer
Mi-Ryung Park,Kyong-Woon Kim,In-Sul Hwang,JeongHee Yun,Tae-Uk Kwak,Ju-Young Lee,Namwoong Hyung,Ji-Hyun Lim,Sang-Hyoun Park,Seongsoo Hwang 한국동물생명공학회(구 한국동물번식학회) 2018 발생공학 국제심포지엄 및 학술대회 Vol.2018 No.06
Tissue-type plasminogen activator, a protein used to treat heart attacks, converts plasminogen into plasmine, which digests fibrin and induces the dissolution of fibrin clots. The purpose of this study was to produce a tPA transgenic pig by somatic cell nuclear transfer. The human tPA (htPA) genes were transfected into porcine ear fibroblast cells derived from a female piglet and established two transgenic cell lines (Tg) with normal karyotypes. Tg line #3 and #4, which expressed high levels of the transgenes. Blastocyst rates of SCNT embryos derived from Tg lines were significantly lower than those of Non-Tg. A total of 2799 SCNT embryos were transferred to 9 surrogates. Seven offspring were delivered, and the production efficiency was 1.0~1.3% (3/324 and 4/304). All offspring exhibited htPA genes. These piglets revealed by microsatellite testing to be genetically identical to Tg cell lines, but not to the surrogate. Our goal is to establish a htPA transgenic pig for studying biopharmaceutical production.