Characterization of endocrine disruption potentials of coastal sediments of Taean, Korea employing H295R and MVLN assays–Reconnaissance at 5years after <i>Hebei Spirit</i> oil spill

Liu, Xiaoshan,Jung, Dawoon,Zhou, Kairu,Lee, Sangwoo,Noh, Kiwan,Khim, Jong Seong,Giesy, John P.,Yim, Un Hyuk,Shim, Won Joon,Choi, Kyungho Elsevier 2018 MARINE POLLUTION BULLETIN Vol.127 No.-

<P><B>Abstract</B></P> <P>Endocrine disrupting potentials were assessed for sediment samples collected near <I>Hebei Spirit</I> oil spill (HSOS) site, between December 2007 and January 2012. For comparison, major crude oil (CO) of HSOS, or its weathered form were assessed. Both raw extracts (REs) and their fractionated samples were tested using H295R and MVLN<I>luc</I> bioassays. In H295R cells, REs of crude and weathered oil (WO), and nine of 14 sediments significantly increased E2 levels, which were correlated with the concentrations of PAHs. Steroidogenic disruption potentials of the sediments generally decreased over time. Among silica fractions of all REs, aromatic hydrocarbons (F2) and polar compounds (F3) caused greater E2 levels. While, in MVLN cell bioassay, only three of 14 sediment REs showed estrogen receptor binding potencies, and no temporal trend was observed. In conclusion, oil spill can cause endocrine disruption in the affected ecosystem through steroidogenic alteration for years, and such potencies attenuate over time.</P> <P><B>Highlights</B></P> <P> <UL> <LI> Endocrine disrupting potentials persist in the sediments of Taean coastline even at 5years after Hebei Spirit Oil Spill. </LI> <LI> However, such potencies of the sediment extracts attenuate over time through weathering. </LI> <LI> Sediments collected caused endocrine disruption potentials through disruption in sex steroidogenesis. </LI> <LI> Aromatic hydrocarbons and polar fractions of the oil spill were responsible for the steroidogenic alteration. </LI> </UL> </P>

miR-30c regulates proliferation, apoptosis and differentiation via the Shh signaling pathway in P19 cells

Xuehua Liu,Mengmeng Li,Yuzhu Peng,Xiaoshan Hu,Jing Xu,Shasha Zhu,Zhangbin Yu,Shuping Han 생화학분자생물학회 2016 Experimental and molecular medicine Vol.48 No.-

MicroRNAs (miRNAs) are small, non-coding single-stranded RNAs that suppress protein expression by binding to the 3′ untranslated regions of their target genes. Many studies have shown that miRNAs have important roles in congenital heart diseases (CHDs) by regulating gene expression and signaling pathways. We previously found that miR-30c was highly expressed in the heart tissues of aborted embryos with ventricular septal defects. Therefore, this study aimed to explore the effects of miR-30c in CHDs. miR-30c was overexpressed or knocked down in P19 cells, a myocardial cell model that is widely used to study cardiogenesis. We found that miR-30c overexpression not only increased cell proliferation by promoting cell entry into S phase but also suppressed cell apoptosis. In addition, we found that miR-30c inhibited dimethyl sulfoxide-induced differentiation of P19 cells. miR-30c knockdown, in contrast, inhibited cell proliferation and increased apoptosis and differentiation. The Sonic hedgehog (Shh) signaling pathway is essential for normal embryonic development. Western blotting and luciferase assays revealed that Gli2, a transcriptional factor that has essential roles in the Shh signaling pathway, was a potential target gene of miR-30c. Ptch1, another important player in the Shh signaling pathway and a transcriptional target of Gli2, was downregulated by miR-30c overexpression and upregulated by miR-30c knockdown. Collectively, our study revealed that miR-30c suppressed P19 cell differentiation by inhibiting the Shh signaling pathway and altered the balance between cell proliferation and apoptosis, which may result in embryonic cardiac malfunctions.

Search-based Path Planning and Receding Horizon Based Trajectory Generation for Quadrotor Motion Planning

Bo Zhang,Pudong Liu,Wanxin Liu,Xiaoshan Bai,Awais Khan,Jianping Yuan 제어·로봇·시스템학회 2024 International Journal of Control, Automation, and Vol.22 No.2

This paper proposes a receding horizon based motion planning method, which allows a sensoryconstrained quadrotor to dynamically plan obstacle-avoiding trajectories in unknown complex environments. First, a two-process search method is proposed to generate an initial feasible path satisfying the dynamics of the quadrotor. Second, the path smoothness is improved by solving a nonlinear optimization problem considering path safety and smoothness. Then, a uniform B-spline is used to interpolate the path with a receding horizon to achieve a safe and dynamically feasible trajectory with minimum trajectory time by solving an optimization problem. Finally, a time adjustment method is proposed based on the relationship between the distance of the B-spline trajectory and the obstacles. Extensive simulation results illustrate that the designed method doubles the safety range, defined as the minimum distance between the quadrotor and the obstacles, and consumes less than 70% of computational running time compared with the state-of-the-art. Outdoor flight experiments performed with a vision-based quadrotor show the satisfying performance of the motion planning approach.

Endocrine disrupting potential of PAHs and their alkylated analogues associated with oil spills

Lee, Sangwoo,Hong, Seongjin,Liu, Xiaoshan,Kim, Cheolmin,Jung, Dawoon,Yim, Un Hyuk,Shim, Won Joon,Khim, Jong Seong,Giesy, John P.,Choi, Kyungho The Royal Society of Chemistry 2017 Environmental science Vol.19 No.9

<▼1><P>Polycyclic aromatic hydrocarbons (PAHs) and alkylated PAHs are known to be major toxic contaminants in spills of petroleum hydrocarbons (oil).</P></▼1><▼2><P>Polycyclic aromatic hydrocarbons (PAHs) and alkylated PAHs are known to be major toxic contaminants in spills of petroleum hydrocarbons (oil). Spilled oil undergoes weathering and over time, PAHs go through a series of compositional changes. PAHs can disrupt endocrine functions, and the type of functions affected and associated potencies vary with the type and alkylation status of PAH. In this study, the potential of five major PAHs of crude oil, <I>i.e.</I>, naphthalene, fluorene, dibenzothiophene, phenanthrene, and chrysene, and their alkylated analogues (<I>n</I> = 25), to disrupt endocrine functions was evaluated by use of MVLN-<I>luc</I> and H295R cell lines. In the MVLN-<I>luc</I> bioassay, seven estrogen receptor (ER) agonists were detected among 30 tested PAHs. The greatest ER-mediated potency was observed for 1-methylchrysene (101.4%), followed by phenanthrene and its alkylated analogues (range of %-E2max from 1.6% to 47.3%). In the H295R bioassay, significantly greater syntheses of steroid hormones were observed for 20 PAHs. For major PAHs and their alkylated analogues, disruption of steroidogenesis appeared to be more significant than ER-mediated effects. The number and locations of alkyl-moieties alone could not explain differences in the types or the potencies of toxicities. This observation shows that disruption of endocrine functions by some constituents of oil spills could be underestimated if only parent compounds are considered in assessments of hazard and risk.</P></▼2>

Genotoxicity and Endocrine-Disruption Potentials of Sediment near an Oil Spill Site: Two Years after the <i>Hebei Spirit</i> Oil Spill

Ji, Kyunghee,Seo, Jihyeon,Liu, Xiaoshan,Lee, Jinyoung,Lee, Sangwoo,Lee, Woojin,Park, Jeongim,Khim, Jong Seong,Hong, Seongjin,Choi, Yeyong,Shim, Won Joon,Takeda, Shunichi,Giesy, John P.,Choi, Kyungho American Chemical Society 2011 Environmental science & technology Vol.45 No.17

<P>The <I>Hebei Spirit</I> oil spill episode (December 7, 2007) has affected the western coastal area of South Korea; however, there is limited information on the potential toxicity of the oil spill to the ecosystem or humans. The potential toxicity of sediments collected from the affected area (<I>n</I> = 22) 2 years after the spill was evaluated. Acute lethal toxicity tests using <I>Vibrio fischeri</I> and <I>Moina macrocopa</I> and tests for genotoxicity and alteration of steroidogenesis using chicken DT40 cells and H295R cells, respectively, were conducted. Both crude and weathered oil extracts were evaluated in order to link the observed toxicity in the sediment extracts to the oil spill. Whereas toxicity to bacteria and daphnids was observed in only two elutriate samples, 10 of the 22 sediment extracts showed genotoxic potential in DT40 cells. The mechanisms of genotoxicity involved nucleotide excision repair (XPA<SUP>–/</SUP>), homologous recombination (RAD54<SUP>–/–</SUP>), and translesion synthesis pathways (REV3<SUP>–/–</SUP>). In addition, nine sediment extracts caused significantly greater production of E2 in H295R cells, and significant up-regulation of CYP19, CYP11B2, and 3βHSD2 by sediment extracts was observed. The pattern of toxicities observed in both crude and weathered oil samples was similar to that observed in the sediment extracts. The genotoxicicity and endocrine-disruption potential of the sediment extracts suggest a need for long-term followup for such toxicity in humans and wildlife in this area.</P><P><B>Graphic Abstract</B> <IMG SRC='http://pubs.acs.org/appl/literatum/publisher/achs/journals/content/esthag/2011/esthag.2011.45.issue-17/es200724x/production/images/medium/es-2011-00724x_0003.gif'></P><P><A href='http://pubs.acs.org/doi/suppl/10.1021/es200724x'>ACS Electronic Supporting Info</A></P>

Layered double hydroxide nanosheets embedded β-cyclodextrin composite membranes with enhanced chiral separation performance

Zhengzhong Zhou,Lingli Zhou,Yi Liu,Qian Wang,Xiaoshan Meng,Qigang Wu,Taoli Huhe 한국공업화학회 2023 Journal of Industrial and Engineering Chemistry Vol.128 No.-

The technologies for enantiomeric resolution are essential to the development of pharmaceutical industry. The membrane technology has great potential in chiral separation owing to its low cost and highthroughput; however, the enantioselective of membrane is limited by the thickness of the active layer,which is closely related to the number of theoretical separation plates. In this work, the layered doublehydroxides (LDH) are synthesized by hydrothermal process and then vacuum filtrated with bcyclodextrin(b-CD) and polyvinyl alcohol (PVA) on a porous substrate to fabricate chiral selective membranes. 2D channels with increased number of separation stages are formed in the selective layer afterheat post-treatment. The enantiomeric excesses value is enhanced from 10.95% to 24.32% for racemicL/D- tryptophan resolution. This work provides a new approach for designing high performance chiralseparation membranes from the aspect of increasing the transport path.

서울시 터널 내 방음벽 설치가 입자상 물질 농도 및 소음 수준에 미치는 영향

조현우,이지연,리우시아오산,이기영,Cho, Hyun-Woo,Lee, Ji-Yeon,Liu, Xiaoshan,Lee, Ki-Young 한국환경보건학회 2010 한국환경보건학회지 Vol.36 No.5

The Seoul metropolitan government has installed soundproofing walls in road tunnels to improve the tunnel environment for pedestrians. To evaluate the efficiency of these soundproofing walls, we measured noise levels and concentrations of $PM_{2.5}$ and ultrafine particles (UFP) in tunnels with or without these walls. Although noise and UFP levels were significantly lower in the tunnels with soundproofing walls, $PM_{2.5}$ levels were significantly higher: but were significantly lowered by use of a ventilation system. When $PM_{2.5}$ and noise levels were simultaneously measured in a tunnel with soundproofing wall, noise level was significantly reduced but $PM_{2.5}$ level was significantly higher. It is concluded that the soundproofing wall can protect pedestrians from noise, but not PM. Installation of a ventilation system is recommended for protection of pedestrians in tunnels with soundproofing walls.

Preparation of Si3N4-based antimicrobial coatings from suspensions with long-time stability

Du Songmo,Zhang Jie,Sun Siyuan,Lifei Zhang,Chen Zhanglin,Zhang Shijia,Zhao Shuo,Tian Zhaobo,Ning Xiaoshan,Chen Kexin,Cui Wei,Liu Guanghua 한국세라믹학회 2024 한국세라믹학회지 Vol.61 No.3

A Si 3N4-based antimicrobial coating was fabricated from suspensions with long-time stability. The stability of Si 3N4 sus- pension and the antimicrobial performance of coating were investigated. The viscosity of Si 3N4 suspension was found to be the key factor aff ecting both stability and antimicrobial performance. The Si 3N4 suspensions were modifi ed with HASE acrylic copolymer, PAA-NH 4, and Polyurethane, resulting in an improvement of the stability of Si 3N4 suspension from a few hours to six months. The coating produced using the modifi ed Si 3N4 suspension exhibited an antibacterial activity of 99.9%. This study highlights the crucial role of modifying the Si 3N4 suspension in improving its long-term stability and coating antimicrobial activity.