The Effect of Polypropylene Fiber and Glass Fiber on the Frost Resistance of Desert Sand Concrete

Lina Hou,Shiliang Jian,Wei Huang 대한토목학회 2024 KSCE Journal of Civil Engineering Vol.28 No.1

To study the influence of polypropylene (PP) fibers and glass fibers on the frost resistance of desert sand concrete (DSC), the fast-freezing tests were carried out to investigate the freeze-thaw damage law using the apparent damage, mass loss, and relative dynamic elastic modulus as indicators; Based on the mercury intrusion method (MIP) and scanning electron microscopy (SEM), the microstructure evolution was analyzed to reveal the mechanism of fiber frost resistance enhancement. Fit the freeze-thaw damage process of fiber reinforced DSC (FRDSC) and predict the frost resistance life. The results show that fibers can greatly improve the freezing resistance of DSC. The hybrid fiber had the highest enhancing effect, followed by PP fiber and glass fiber. The dynamic elastic modulus of the DSC with 0.15% PP fiber and 0.05% glass fiber is as high as 95%. The pore distribution ratio of FRDSC changes faster, but it is still better than that of reference DSC after freeze-thaw. PP fiber and glass fiber can play a full role in the micro and macro stages of crack development of DSC respectively. The freeze-thaw damage model has high fitting accuracy and the DSC mixed with fibers can significantly improve the service life of buildings in northern China.

지식재산권 보호가 수입에 미치는 영향 : OECD 회원국과 비OECD 회원국간 비교

후립나(Lina Hou),장선미(Sun-Mi Chang) 한국통상정보학회 2021 통상정보연구 Vol.23 No.4

지식재산권보호가 무역에 미치는 영향은 시장확대효과(market expansion effect)와 시장지배력효과(market power effect)의 두 가지로 나눌 수 있다. 본 연구에서는 전세계 122개국가를 대상으로 지식재산권보호가 수입에 미치는 영향을 실증분석하였다. 분석연도는 2000년, 2005년, 2010년, 2015년이며, 전체국가를 OECD 회원국과 비OECD 회원국으로 구분하여 지식재산권의 보호효과에 차이가 있는지도 살펴보았다. 분석결과 전체국가를 대상으로 하였을 때는 국가의 지식재산권 보호의 수준이 높을수록 수입에 긍정적 영향을 미치는 것으로 나타나 지식재산권의 보호가 무역에 미치는 시장확대효과를 지지하는 것으로 나타났다. 국가의 규모(GDP), 개방도, R&D집중도를 모형에 추가하여 분석한 결과 국가의 규모와 개방도는 무역에 유의한 양의 영향을 미치는 것으로 나타났으나 R&D집중도의 영향은 유의하지 않은 것으로 나타났다. 이러한 효과는 OECD 회원국을 대상으로 했을 때 더욱 분명하게 나타나며, 비 OECD 회원국의 경우 지식재산권이 무역에 미치는 영향은 확실하지 않다. 또한 OECD회원국을 대상으로 규모(GDP)와 개방도와 R&D집중도 변수를 추가하여 분석한 결과 이들 변수는 모두 수입에 유의한 양의 영향을 미치는 것으로 나타났다. The impact of intellectual property right(IPR) protection on trade can be divided into two categories: the market expansion effect and the market power effect. This study empirically analyzed the impact of IPR protection on imports in 122 countries. The years of analysis are 2000, 2005, 2010, and 2015. We also examined whether there are differences in the protection effect of IPRs between OECD and non-OECD member countries. As a result of the analysis for all countries, the higher the level of national IPR protection, the more positive the impact on imports, thus supporting the market expansion effect of IPR protection on trade. As a result of analyzing the country s size(GDP), openness, and R&D intensity by adding it to the model, it was found that the country s size and openness had a significant effect on trade, but the effect of R&D intensity was not significant. This effect is more evident in OECD member countries, and the impact of intellectual property rights on trade in non-OECD member countries is unclear. In addition, as a result of analyzing the scale(GDP), degree of openness, and R&D intensity in OECD member countries, it was found that all of these variables had a significant positive effect on imports.

Expression and Detection of Retinol-Binding Protein-4 Gene of Pig in E. coli

LiNa Sun,WanHong Li,ShuXiong Chen,Chao Chen,XiaoFeng Hou,Yun Zhao,Lu Chen,ChunJin Li,Xu Zhou 한국동물생명공학회(구 한국동물번식학회) 2014 한국동물번식학회 한중일 심포지엄 Vol.2014 No.1

Retinol-Binding Protein-4 (RBP-4) is a low molecular weight lipocalin, which mainly functions as a carrier for vitamin A. Though liver is the main machinery for synthesis of this protein, it is also detectable in other extrahepatic tissues, for example, ovary, uterus, and placenta. Recent evidences have shown that RBP-4 plays important roles in animal reproduction, for example, promoting the development of uterus and embryo. To the best of our knowledge, our laboratory firstly reported that high level of RBP-4 existed in follicular fluid from follicular cysts in sows. Moreover, we have also found that RBP-4 could be secreted by granulosa cells, and RBP-4 receptor was detected in granulosa cells. However, there is no any evidence on the role of RBP-4 in regulating the follicular development. Therefore, cloning and expression of RBP-4 and preparation of polyclonal antibody could help us to explore the role of RBP-4 in follicular development. The aim of this work was to construct prokaryotic expression system of swine RBP-4 gene. The total RNA was extracted from swine’s normal ovarian tissue. The sequence including the whole length of RBP-4 was amplified by RT-PCR and inserted into pEASY-E1.Then transformed into E. coli BL21(DE3)pLysS after gene sequencing. Three hours later, adding IPTG with the final concentration of 1mmol/L and inducing five hours. After centrifugation, the supernatant was discarded. By adding Glucose to Luria-Bertani broth, the expressions of protein were increased. SDS-PAGE showed that the RBP-4 gene expressed in the form of inclusion body with a molecular weight of 21KD. Western-Blot results showed that the target protein could be specifically recognized by mouse anti-human monoclonal antibody. Prokaryotic expression vector of RBP-4 gene was successfully established, and the gene was successfully expressed n E. coli, which is ready for purification and RBP-4 polyclone antibody. Meanwhile, these results were beneficial to investigate the function of RBP-4 in follicular development.

MiR-339 attenuates LPS-induced intestinal epithelial cells inflammatory responses and apoptosis by targeting TLR4

Meiying Xie,Lina Zhang,Luoye Li,Minhuan Fan,Lianjie Hou 한국유전학회 2020 Genes & Genomics Vol.42 No.9

Background Intestinal epithelial cells are important for defending against pathogen infection. LPS is an endotoxin that is highly antigenic and cytotoxic produced by bacteria. LPS disrupts the intestine epithelium integrity and induced the intestinal epithelial cell infammation and apoptosis. Our previous study has predicted the function of exosome miRNAs through bioinformatics analysis, and we found that miR-339 had a potential function in cell infammation response. To our knowledge, no published paper has demonstrated the miR-339 function in protecting the intestine epithelium against bacterial infection. Objective The objective of this study is to evaluate the miR-339 function in regulating intestinal epithelial cells to defend against bacterial infection through biological experiments and bioinformatics analyses. Methods Through the miR-339 transfection experiment and TLR4 interfering experiment, we evaluated the function of miR339 and TLR4 in the process of infammatory responses and apoptosis. Through Bioinformatics analyses and dual-luciferase reporter experiment, we identifed the target gene of miR-339. Results miR-339 attenuates LPS-induced intestinal epithelial cells infammatory responses through the TLR4/NF-κB signaling pathway and inhibited LPS-induced apoptosis through the P53 signaling pathway. TLR4 is the target gene of miR-339. TLR4 reduced LPS-induced proinfammatory responses and apoptosis. Conclusions In conclusion, miR-339 protected the intestine epithelial cells from LPS-induced cell infammation and apoptosis through targeting TLR4. This study expanded our understanding of how miRNAs and genes work collaboratively in regulating intestinal epithelial cells to defend against bacterial infection.

Ginseng berry polysaccharides on infl ammation-associated colon cancer: inhibiting T-cell differentiation, promoting apoptosis, and enhancing the effects of 5-fl uorouracil

Chong-Zhi Wang,Lifei Hou,Jin-Yi Wan,Haiqiang Yao,Jinbin Yuan,Jinxiang Zeng,Chan Woong Park,Su Hwan Kim,Dae Bang Seo,Kwang-Soon Shin,Chun-Feng Zhang,Lina Chen,Qi-Hui Zhang,Zhi Liu,Clara Sava-Segal,Chun 고려인삼학회 2020 Journal of Ginseng Research Vol.44 No.2

Background: Ginseng is a commonly used herbal medicine in treating various medical conditions. Chronic gut inflammation is a recognized factor for the development of colorectal cancer (CRC). In thisproject, Asian ginseng berry polysaccharide preparations were used to assess their effects on CRC andrelated immune regulation mechanisms. Methods: Ginseng berry polysaccharide extract (GBPE) and purified ginseng berry polysaccharideportion (GBPP) were used to evaluate their activities on human HCT-116 and HT-29 CRC cell proliferation. Interleukin-8 secretion analysis was performed on HT-29 cells. Naive CD4 cell isolation and T-helper celldifferentiation were performed and determined using flow cytometry for Th1 and Treg in addition to cellcycle and apoptotic investigation. Results: GBPE and GBPP significantly inhibited interleukin-8 secretion and cancer cell proliferation,inhibited CD4þIFN-gþ cell (Th1) differentiation, and decreased CD4þFoxP3þ cell (Treg) differentiation. Compared to the GBPE, GBPP showed more potent antiinflammatory activities on the malignant cells. This is consistent with the observation that GBPP can also inhibit Th1-cell differentiation better, suggestingthat it has an important role in antiinflammation, whereas Treg cells hinder the body’s immuneresponse against malignancies. Supported by cell cycle and apoptosis data, GBPE and GBPP, at variousdegrees, remarkably enhanced the anticancer activities of 5-fluorouracil. Conclusion: Data from this project suggested that Asian ginseng berry potentially has clinical utility inmanaging enteric inflammation and suppressing CRC through immunomodulation mechanisms.

Ginseng berry polysaccharides on inflammation-associated colon cancer: inhibiting T-cell differentiation, promoting apoptosis, and enhancing the effects of 5-fluorouracil

Wang, Chong-Zhi,Hou, Lifei,Wan, Jin-Yi,Yao, Haiqiang,Yuan, Jinbin,Zeng, Jinxiang,Park, Chan Woong,Kim, Su Hwan,Seo, Dae Bang,Shin, Kwang-Soon,Zhang, Chun-Feng,Chen, Lina,Zhang, Qi-Hui,Liu, Zhi,Sava-Se The Korean Society of Ginseng 2020 Journal of Ginseng Research Vol.44 No.2

Background: Ginseng is a commonly used herbal medicine in treating various medical conditions. Chronic gut inflammation is a recognized factor for the development of colorectal cancer (CRC). In this project, Asian ginseng berry polysaccharide preparations were used to assess their effects on CRC and related immune regulation mechanisms. Methods: Ginseng berry polysaccharide extract (GBPE) and purified ginseng berry polysaccharide portion (GBPP) were used to evaluate their activities on human HCT-116 and HT-29 CRC cell proliferation. Interleukin-8 secretion analysis was performed on HT-29 cells. Naive CD4 cell isolation and T-helper cell differentiation were performed and determined using flow cytometry for Th1 and Treg in addition to cell cycle and apoptotic investigation. Results: GBPE and GBPP significantly inhibited interleukin-8 secretion and cancer cell proliferation, inhibited CD4⁺IFN-γ⁺ cell (Th1) differentiation, and decreased CD4⁺FoxP3⁺ cell (Treg) differentiation. Compared to the GBPE, GBPP showed more potent antiinflammatory activities on the malignant cells. This is consistent with the observation that GBPP can also inhibit Th1-cell differentiation better, suggesting that it has an important role in antiinflammation, whereas Treg cells hinder the body's immune response against malignancies. Supported by cell cycle and apoptosis data, GBPE and GBPP, at various degrees, remarkably enhanced the anticancer activities of 5-fluorouracil. Conclusion: Data from this project suggested that Asian ginseng berry potentially has clinical utility in managing enteric inflammation and suppressing CRC through immunomodulation mechanisms.

Parallel task scheduling under multi-Clouds

( Yongsheng Hao ),( Mandan Xia ),( Na Wen ),( Rongtao Hou ),( Hua Deng ),( Lina Wang ),( Qin Wang ) 한국인터넷정보학회 2017 KSII Transactions on Internet and Information Syst Vol.11 No.1

In the Cloud, for the scheduling of parallel jobs, there are many tasks in a job and those tasks are executed concurrently on different VMs (Visual machines), where each task of the job will be executed synchronously. The goal of scheduling is to reduce the execution time and to keep the fairness between jobs to prevent some jobs from waiting more time than others. We propose a Cloud model which has multiple Clouds, and under this model, jobs are in different lists according to the waiting time of the jobs and every job has different parallelism. At the same time, a new method-ZOMT (the scheduling parallel tasks based on ZERO-ONE scheduling with multiple targets) is proposed to solve the problem of scheduling parallel jobs in the Cloud. Simulations of ZOMT, AFCFS (Adapted First Come First Served), LJFS (Largest Job First Served) and Fair are executed to test the performance of those methods. Metrics about the waiting time, and response time are used to test the performance of ZOMT. The simulation results have shown that ZOMT not only reduces waiting time and response time, but also provides fairness to jobs.