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- 저자 : Li Liulin (검색결과 3 건)
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MicroRNA‑377‑3p inhibits growth and invasion through sponging JAG1 in ovarian cancer
Liulin Tang,Bin Yang,Xiaolan Cao,Qin Li,Li Jiang,Dan Wang 한국유전학회 2019 Genes & Genomics Vol.41 No.8
Background Ovarian cancer is the one of the most deadly gynecologic malignancy among cancer related death in women. However, the treatment for ovarian cancer is still limited. In this study, we aimed to explore the inhibition potential of miR- 377-3p in ovarian cancer and explore the mechanism of this effect. Methods Quantitative real-time PCR was used to detect the mRNA or microRNA (miRNA) levels. CCK-8, wound-healing, transwell assay were used to detect cell proliferation, migration and invasion. The protein levels were examined by western blot. The dual luciferase reporter assay was conducted to examine the luciferase activity. Tumor volume was measured and Ki67 was detected via immunohistochemistry. Results qRT-PCR results showed that miR-377-3p was downregulated in ovarian cancer patients. MiR-377-3p mimics suppressed cell proliferation, migration, invasion and decreased the JAG1 level. However, miR-377-3p inhibitor promoted these appearances. Interestingly, we found JAG1 was a target gene of miR-377-3p. JAG1 overexpression reversed the miR- 377-3p-induced inhibition of proliferation and invasion. In addition, miR-377-3p inhibited ovarian cancer tumorigenesis in vivo, indicating by decreased tumor volume and staining of Ki67. Conclusion The results showed that miR-377-3p inhibited growth and invasion of ovarian cancer cells by targeting JAG1.
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Liu Shaohua,Li Junhao,Li Na,Zhou Peng,Li Liulin 한국원예학회 2022 Horticulture, Environment, and Biotechnology Vol.63 No.6
Calcium-dependent protein kinases (CDPKs) play signifi cant roles in response to environmental stresses in plants. However, the CDPK gene family in pear ( Pyrus bretschneideri Rehd) has not been thoroughly explored. In this study, we performed a comprehensive analysis of CDPK family genes in pear, and 30 CDPK genes were identifi ed, unevenly distributed across 13 chromosomes, and predicted to be mainly localized at the plasma membrane. Based on phylogenetic analysis and struc- tural features, the PbCDPKs were classifi ed into four distinct subfamilies. In addition, many putative cis-acting elements responsive to hormones and environmental stresses were identifi ed in the promoter regions. According to RNA-seq data, the majority of PbCDPK genes showed diff erential expression in diff erent pear tissues. After inoculation of plants with the fungus Venturia nashicola , four PbCDPK genes were diff erentially expressed in resistant and susceptible pear cultivars, suggesting that they might play an important role in resistance to the pear scab pathogen. These candidate genes will be subjected to disease resistance and functional analysis in future work. Our results thus provide a genome-wide characterization of CDPK family genes in pear and lay the foundation for further functional analysis.
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Candidate gene mining of GA-mediated regulation of pear fruit shape
Xue Zeyu,Yang Ru,Wang Yadong,Ma Yuchen,Lin Yujing,Li Zhengao,Song Yuqin,Feng Xinxin,Li Liulin 한국원예학회 2024 Horticulture, Environment, and Biotechnology Vol.65 No.3
Fruit shape is one of the important traits for quality evaluation, classifi cation, and market grading of horticultural crops. In order to explore the genes regulating pear fruit shape formation, pear varieties ‘Huangguan’ and ‘Korla Fragrant Pear’ with signifi cant diff erences in fruit shape were selected as experimental materials. Through morphological observation, measurement of fruit longitudinal diameter and fruit transverse diameter, and fruit shape index analysis of the pear fruit growth and development period, the formation of pear fruit shape during the young fruit period was preliminarily determined. By analyzing the transcriptome data of ‘Huangguan’ and ‘Korla Fragrant Pear’ at the young fruit stage, 8993 diff erentially expressed genes were obtained, and the majority of the genes were annotated to the plant hormone signaling pathway, among which gibberellin-related genes accounted for 13.4%. Spraying GA 3 at the full-bloom stage can signifi cantly prolong the shape of oriental pear. The results of qRT-PCR showed that the expression level of the gibberellin metabolic pathway gene PbGA2ox11 in ‘Huangguan’ was signifi cantly higher than that in ‘Korla Fragrant Pear’. GA 3 treatment inhibited the expression of this gene in ‘Korla Fragrant Pear’, while PP 333 promoted the expression. It was speculated that PbGA2ox11 was most likely to regulate the shape of pear fruit by regulating gibberellin metabolism. This study provides a new method for studying fruit shape via the analysis of the gibberellin synthesis metabolic pathway, new resources for the rational use of plant growth regulators, and theoretical and technical support for cultivating excellent fruit shape.
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