MiR-886-5p Inhibition Inhibits Growth and Induces Apoptosis of MCF7 Cells

Zhang, Lei-Lei,Wu, Jiang,Liu, Qiang,Zhang, Yan,Sun, Zhu-Lei,Jing, Hong Asian Pacific Journal of Cancer Prevention 2014 Asian Pacific journal of cancer prevention Vol.15 No.4

Background and Aims: To explore the molecular mechanisms of miR-886-5p in breast cancer., we examined roles in inhibiting growth and migration of MCF-7 cells. Methods: MiR-886-5p mimics and inhibitors were used to express or inhibit MiR-886-5p, respectively, and MTT and clone formation assays were used to determine the survival and proliferation. Hoechst 33342/ PI double staining was applied to detect apoptosis. The expression of caspase-3, caspase-8, caspase-9, MT1-MMP, VEGF-C and VEGF-D was detected by Western blotting, and the levels of MMP2 and MMP9 secreted from MCF-7 cells were assessed by ELISA. MCF-7 cell migration was determined by wound healing and Transwell assays. Results: We found that the growth of MCF-7 cells was inhibited upon decreasing miR-886-5p levels. Inhibiting miR-866-5p also significantly induced apoptosis and decreased the migratory capacity of these cells. The expression of VEGF-C, VEGF-D, MT1-MMP, MMP2, and MMP9 was also found to be decreased as compared to controls. Conclusions: Our data show that downregulation of miR-886-5p expression in MCF-7 cells could significantly inhibit cell growth and migration. This might imply that inhibiting miR-886-5p could be a therapeutic strategy in breast cancer.

The Effect of Impeller Indent Distance on the Performance of Vortex Pumps

Lei DAI,Lei GU,Jun WANG,Xiang AO,Xiaobo ZHU 한국유체기계학회 2020 International journal of fluid machinery and syste Vol.13 No.1

Low efficiency is an urgent problem to be solved for vortex pumps due to large number of vortices and backflows. This paper concentrates on the performance improvement of vortex pumps caused by changing the indent distance of the impeller. First, a complete numerical model was established by selecting the optimal length of inlet and outlet and appropriate mesh quantity. Then, the reliability of this model was validated by comparing simulation results with experimental data. Based on this model, the internal flows of vortex pumps with different impeller indent distances were observed in simulations and the fitted curves of head and efficiency were created. Finally, the cause of the performance improvement was demonstrated by analyzing the flow field in vortex pumps.

Comparative study of small crack growth behavior between specimens with and without machining-induced residual stress of alloy GH4169

Lei Zhu,Zhirong Wu,Xuteng Hu,Yingdong Song 대한기계학회 2018 JOURNAL OF MECHANICAL SCIENCE AND TECHNOLOGY Vol.32 No.11

We investigated the small fatigue crack behavior of alloy GH4169 by using single-edge-notch tension specimens. Residual stress introduced by machining process was taken into consideration, and two stress levels were selected. A comparison was made between the experimental results of specimens with and without machining-induced residual stress. The results indicated that fatigue cracks of the two types of specimens initiated from surface inclusions or grain boundaries. For both types of specimens, small cracks grew very slowly when the crack lengths were less than 500 μm. The small crack growth might decelerate and retard temporarily for the existence of grain boundaries. The residual stress effect on crack growth can be identified at σ max = 380 MPa, i.e., compressive residual stress might impede the crack growth. However, this phenomenon was indistinguishable at σ max = 410 MPa.

In vivo optical imaging of membrane-type matrix metalloproteinase (MT-MMP) activity.

Zhu, Lei,Zhang, Fan,Ma, Ying,Liu, Gang,Kim, Kwangmeyung,Fang, Xuexun,Lee, Seulki,Chen, Xiaoyuan American Chemical Society 2011 Molecular pharmaceutics Vol.8 No.6

<P>Herein we demonstrate for the first time that a fluorogenic probe can be used as an in vivo imaging agent for visualizing activities of membrane-tethered, membrane-type matrix metalloproteinases (MT-MMPs). An MT-MMP fluorogenic probe that consisted of an MT1-MMP (MMP-14) substrate and near-infrared (NIR) dye-quencher pair exhibited rapid, efficient boosts in fluorescence upon cleavage by MT1-MMP in tumor-bearing mice. In particular, unlike similar fluorogenic probes designed to target extracellular, soluble-type MMPs (EC-MMPs)--which can be cleared from the bloodstream after activation--the fluorescence signals activated by MT1-MMP enable clear visualization of MT1-MMP-positive tumors in animal models for up to 24 h. The results indicate that a simple form of a fluorogenic probe that is less effective in EC-MMP imaging is an effective probe for imaging MT-MMP activities in vivo. These findings can be widely applied to designing probes and to applications targeting various membrane-anchored proteases in vivo.</P>

Hydrothermal synthesis and highly visible light-induced photocatalytic activity of acid functionalized MWCNTs as support for ZnS-photosensitized TiO2 catalysts

Lei Zhu,임창성,Ze-Da Meng,최종근,박종연,Trisha Ghosh,조광연,오원춘 한양대학교 세라믹연구소 2012 Journal of Ceramic Processing Research Vol.13 No.3

Nanoscale ZnS-photosensitized TiO2 particles supported on acid functionalized multi-wall carbon nanotubes (MWCNTs) were prepared via a mild hydrothermal route. The new optical properties and porous structure of the as-prepared samples were characterized by transmission electron microscopy (TEM), scanning electron microscopy (SEM), BET surface area analysis,UV-vis DRS and X-ray diffraction (XRD). The photocatalytic activities of the composite catalysts were evaluated by the decomposition of an MB solution under visible light irradiation. The results indicate that the addition of an appropriate amount of MWCNTs as supports for TiO2 and ZnS efficiently inhibit the agglomeration of ZnS/TiO2 thus improving the dispersion of nanoparticles and the activity of visible photodegradation was remarkably improved.

FDG PET-CT in Non-small Cell Lung Cancer: Relationship between Primary Tumor FDG Uptake and Extensional or Metastatic Potential

Zhu, Shou-Hui,Zhang, Yong,Yu, Yong-Hua,Fu, Zheng,Kong, Lei,Han, Da-Li,Fu, Lei,Yu, Jin-Ming,Li, Jia Asian Pacific Journal of Cancer Prevention 2013 Asian Pacific journal of cancer prevention Vol.14 No.5

Objective: To explore the relationships between primary tumor $^{18}F$-FDG uptake measured as the SUVmax and local extension, and nodal or distant organ metastasis in patients with NSCLC on pretreatment PET-CT. Methods: 93 patients with NSCLC who underwent $^{18}F$-FDG PET-CT scans before the treatment were included in the study. Primary tumor SUVmax was calculated; clinical stages, presence of local extension, nodal and distant organ metastases were recorded. The patients with SUVmax${\geq}2.5$ were divided into low and high SUVmax groups by using the median SUVmax. The low SUVmax group consisted of 45 patients with SUVmax<10.5, the high SUVmax group consisted of 46 patients with SUVmax${\geq}10.5$. Their data were compared statistically. Results: 91 cases with SUVmax${\geq}2.5$ were included for analysis. The mean SUVmax in patients without any metastasis was $7.42{\pm}2.91$ and this was significantly lower than that ($12.18{\pm}4.94$) in patients with nodal and/or distant organ metastasis (P=0.000). In the low SUV group, 19 patients had local extension, 22 had nodal metastasis, and 9 had distant organ metastasis. In the high SUV group, 31 patients had local extension, 37 had nodal metastasis, and 18 had distant organ metastases. There was a significant difference in local extension (P =0.016), distant organ metastasis (P =0.046), and most significant difference in nodal metastasis rate (P =0.002) between the two groups. In addition, there was a moderate correlation between SUVmax and tumor size (r = 0.642, P<0.001), tumor stage (r = 0.546, P<0.001), node stage (r = 0.388, P<0.001), and overall stage (r = 0.445, P= 0.000). Conclusion: Higher primary tumor SUVmax predicts higher extensional or metastatic potential in patients with NSCLC. Patients with higher SUVmax may need a close follow-up and more reasonable individual treatment because of their higher extensional and metastatic potential.

Analysis on the Operation of a Charging Station with Battery Energy Storage System

Lei Zhu,Yongjian Pu 대한전기학회 2017 Journal of Electrical Engineering & Technology Vol.12 No.5

Fossil oil, as the main energy of transportation, is destined to be exhausted. The electrification of transportation is a sustainable solution to the energy crisis, since electric power could be acquired from the inexhaustible sun, wind and water. Among all the problems that hinder the development of Electric Vehicle (EV) industry, charging issue might be the most prominent one. In this paper, the service process of a charging station with Battery Energy Storage System (BESS) is analyzed by means of Cramér – Lundberg model which has been intensively utilized in ruin theory. The service quality is proposed in two dimensions: the service efficiency and the service reliability. The arrival rate and State of Charge (SOC) upon arrival are derived from 2009 National Household Travel Survey (NHTS). The simulations are performed to show how the service quality is determined by the system parameters such as the number of servers, the service rate, the initial capacity, the charge rate and the maximum waiting time. At last, the economic analysis of the system is conducted and the best combination of the system parameters are given.

Effects of Exogenous Abscisic Acid on Phenolic Characteristics of Red Vitis vinifera Grapes and Wines

Lei Zhu,Yali Zhang,Wen Zhang,Jiang Lu 한국식품과학회 2016 Food Science and Biotechnology Vol.25 No.2

The effects of exogenous abscisic acid (ABA) on phenolic characteristics of grapes and wines were investigated in Vitis vinifera cvs. Merlot and Cabernet Sauvignon. Exogenous ABA treatment at veraison significantly improved phenolic contents, mainly anthocyanins and flavonols, and antioxidant properties of the grape skins, but had no effects on total phenolics and antioxidant activities in the seeds or on basic fruit qualities. The wines made from ABA-treated grapes were also consequently enhanced in total phenolics, anthocyanins, flavonols and antioxidant activities. The proportions of methylated anthocyanins in the skins and acylated anthocyanins and derived pigments in the wines were decreased to some degree by exogenous ABA treatment. No distinct relationships were observed between ABA concentrations and phenolic characteristics, and the effects were observed even with 200 mg/L ABA. The results revealed that exogenous ABA applied at veraison offered opportunities to improve phenolic contents and nutritional values of grape skins and wines.

Ag2Se Modified TiO2 Heterojunction with Enhanced Visible-Light Photocatalytic Performance

Lei Zhu,Jia-Yao Tang,Jia-Yi Fan,Chen-Sun,Won-Chun Oh 한국재료학회 2021 한국재료학회지 Vol.31 No.12

To build a highly active photocatalytic system with high efficiency and low cast of TiO2, we report a facile hydrothermal technique to synthesize Ag2Se-nanoparticle-modified TiO2 composites. The physical characteristics of these samples are analyzed by X-ray diffraction, scanning electron microscopy with energy dispersive X-ray analysis, transmission electron microscopy and BET analysis. The XRD and TEM results show us that TiO2 is coupled with small sized Ag2Se nanoplate, which has an average grain size of about 30 nm in diameter. The agglomeration of Ag2Se nanoparticles is improved by the hydrothermal process, with dispersion improvement of the Ag2Se@TiO2 nanocomposite. Texbrite BA-L is selected as a simulated dye to study the photodegradation behavior of as-prepared samples under visible light radiation. A significant enhancement of about two times the photodegradation rate is observed for the Ag2Se@TiO2 nanocomposite compared with the control sample P25 and as-prepared TiO2. Long-term stability of Ag2Se@TiO2 is observed via ten iterations of recycling experiments under visible light irradiation.

Enhanced Photocatalytic Efficiency of Nanoscale NiS2/TiO2 Catalysts Synthesized by Hydrothermal and Sol-gel Method

Lei Zhu,Ze-Da Meng,Trisha Ghosh,오원춘 한국세라믹학회 2012 한국세라믹학회지 Vol.49 No.2

To improve the visible-light induced photocatalytic application performances of TiO2, in this study, the NiS2 modied TiO2 composites were prepared by two methods: hydrothermal method and sol–gel method. The composites were denoted as hs-NiS2/TiO2, and sg-NiS2/TiO2 and characterized by XRD, UV-vis absorbance spectra, SEM, TEM, EDX, and BET analysis. The photocatalytic activities under visible light were investigated by the degradation of methyl orange (MO). The photodegradation rate of methyl orange under visible light with NiS2/TiO2 composites was markedly higher than that of pure TiO2, and the effect of hs-NiS2/TiO2 composites was better than that of sg-NiS2/TiO2. The results indicate that the hydrothermal process could partly inhibit the agglomeration of NiS2/TiO2. Thus, the dispersion of nanoparticles was improved, and that the promoting effect of NiS2 could extend the light absorption spectrum toward the visible region.