8-hydroxyguanine이 전립선비대증 발생에 미치는 효과

이재원,이석영,이형래,이상철,김용태,김원재 충북대학교 의학연구소 2002 忠北醫大學術誌 Vol.12 No.2

연구목적: 전립선비대증은 노화현상에 의해 발생하는 질환으로서 임상적으로 주로 50세 이상에서 증상이 나타난다. 산소 라디칼은 DNA의 염기에 다양한 손상을 미칠 수 있다. 산소 라디칼에 의한 DNA의 변화에는 여러 가지 형태가 있으며 이러한 변화들 가운데서 guanine이 8-oxoguanine (8-hydroxyguanine, oh8Gua)으로의 수산화(hydroxylation) 반응은 가장 흔히 관찰되는 형태로서 노화 및 만성 퇴행성 질환, 기타 여러 질환의 중요한 병인으로 인식되고 있다. 세포에 유해한 영향을 주는 oh8Gua의 생성으로부터 유전자를 보호하는 수복효소 중 8-oxoguanine-DNA glycosylase (OGG1)가 주된 역할을 하는 것으로 알려져 있는데 이 효소를 생산하는 유전자인 hOGG1은 사람마다 유전자형이 다르다. 따라서 본 연구에서는 전립선비대증이 노화와 관련된 hOGG1 유전자형에 따라서 발생에 차이가 있는지를 조사하였다. 대상 및 방법: 충북대학교 병원에서 전립선비대증으로 진단 받고 입원하여 경요도전립선절제술을 받은 환자로 병리 조직학적으로 전립선비대증이 확인된 81명을 환자군으로 하였으며, 충청북도내 10개 군에서 50세 이상의 건강한 성인 남자 764명을 대상으로 실시한 전립선 무료검진에서 경직장 초음파 검사상 전립선 크기 20 gm 이하, 국제 전립선 증상 점수(IPSS) 7점 이하, 요속 10 ㎖/s 이상, 전립선 특이 항원(PSA) 4.0 ng/㎖ 이하인 81명을 대조군으로 설정하였다. 이들에서 채취한 혈액에서 genomic DNA를 얻은 후 PCR-SSCP (polymerase chain reaction-single stranded conformation polymorphism), 직접 염기서열분석(direct DNA sequencing) 및 RFLP (restriction fragment length polymorphism)를 통해서 두 군 사이의 hOGG1 유전자의 다형성을 분석하였다. 결과: 대조군에서 codon 326형은 Ser326Ser형이 12례(14.8%), Ser326Cys형이 51례(63.0%), Cys326Cys형이 18례(22.2%)였으며 전립선비대증 환자군에서는 Ser326Ser형이 15례(18.5%), Ser326Cys형이 34례(42.0%), Cys326Cys형이 32례(39.5%)로 두 군 사이에 통계적인 유의성이 관찰되었다(p=0.022). 또한 Cys326Cys형과 나머지(Ser326Ser, Ser326Cys)형으로 나누어 시행한 통계 분석에서도 대조군에서는 각각 18례(22.2%), 63례(77.8%)였으며 전립선비대증 환자군에서는 32례(39.5%), 49례(60.5%)로 통계적인 유의성이 관찰되었다(p=0.017, OR=2.286, 95% CI=1.149-4.546). 결론: hOGG1 유전자의 형태와 전립선비대증의 발생과는 밀접한 관련이 있었다. 즉 hOGG1 Cys326Cys형을 가진 사람에서 전립선비대증이 발생한 가능성이 높을 것으로 사료된다. Purpose: 8-oxoguanine DNA glycosylase (OSS1) rapairs DNA by removing 8-oxoguanine (oh8Gua), a highly mutagenic oxidative DNA adduct. Recently, the human gene for OGG1 (hOGG1) was cloned and several genotypes have been reported. However, the implications of such genotypes in benign prostatic lyperplasia have not been demonstrated. To assess the involvement of hOGG1 associated with the aging process on the developing of BPH, we analyzed the genetic polymorphisms of hOGG1. Materials and Methods: We studied the hOGG1 gene polymorphisms in 81 cases of BPH and 81 cases of normal controls. We performed the SSCP, PCR-based restriction fragment length polymorphism (RFLP) and direct sequencing to characterize the genetic polymorphisms of hOGG1 in both BPH patients and controls. Results: We found two polymorphic sites. A serine326/cysteine polymorphism at codon 326 (1a type) in exon 7 was associated with an exchange of amino acid. Another polymorphic site at codon 324 (1b type) in exon 6 was silent. We found that the distribution of hOGG1 genotypes in BPH patients (Ser326Ser type 18.5%, Ser326Cys type 42.0% and Cys326Cys type 39.5%) was significantly different from that in controls (14.8%, 63.0% and 22.2%, respectively) (p=0.022). Homozygosity for the Cys326Cys genotype significantly increased the risk of developing BPH, with the odds ratio (OR) being 2.286 (95% confidence interval [CI]=1.149-4.546) Conclusion: Our results suggest that the hOGG1 Cys326Cys genotype might play a important role in the development of BPH.

온열요법과 기치유요법이 어깨근통증 압통역치에 미치는 영향

이석복 ( Lee Seok Bok ),이민선 ( Lee Min Sun ),이형환 ( Hyung H Lee1 ) 한국자연치유학회 2014 Journal of Naturopathy Vol.3 No.1

In this study, we wanted to understand the influence of thermotherapy, Qi-therapy, and their combination therapy on the oppressive muscular pains in shoulders. For the clinical study, 102 subjects were divided into 3 groups of thermotherapy, Qi-therapy, and the combination therapy to see the differences in the therapeutic effects. The treatments were taken 5 times for 2 weeks in 3-day intervals and pre- and post-treatment assessments were undertaken for the visual pain assessment (VAS, visual analogue scale) and experimental pressure threshold in 8 points in the relevant muscles (PPT). VAS score was decreased by 39% after thermotherapy, 66.5% after Qi-therapy, and 72.3% after combination therapy, all with significance (p<.05). The experimental pressure thresholds of shoulder muscles, the upper trapezius muscles, the lower trapezius muscles, the anterior trapezius muscles. the supraspinous muscles, the greater pectoral muscles, and the sternocleidomastoid muscles were increased by 4.3~11.3% after thermotherapy, 5.9~19.9% after Qi-therapy, and 9.3~25.4% after combination therapy. The experimental pressure thresholds in the 8 points in the relevant muscles did not show a significant difference between the Qi-therapy and combination therapy in the upper and lower trapezius muscles, however, did show a significant difference to the thermotherapy. In the shoulder muscles, anterior and posterior trapezius muscles, supraspinous muscles, supraspinous muscles, greater pectoral muscles, and sternocleidomastoid muscles, there were significant superiorities in Qi-therapy over thermo therapy, and in the combination therapy over Qi-therapy. In conclusion, it could be understood that both thermotherapy and Qi-therapy have been effective in the improvement of pains, and especially combination therapy showed better effectiveness. It is expected to apply these natural therapies to the pain treatment of human body to contribute to the improvement of health and welfare.

전기 기계적 해리로부터 회복된 쯔쯔가무시병에서의 급성 전격성 심근염

정영학,이민주,이경화,이지훈,김석형,이병권 이화여자대학교 의과학연구소 2016 EMJ (Ewha medical journal) Vol.39 No.1

Scrub typhus, caused by Orientia tsutsugamushi , is an acute febrile illness. Characteristics of tsutsugamushi disease are fever, rash and eschar. However, severe complications might rarely occur, such as acute fulminant myocarditis caused by scrub typhus. Thus, there are few reports of recovery from seriously complicated cases. We report on an adult male with scrub typhus complicated with acute fulminant myocarditis with no previous comorbid illness who recovered successfully with proper treatment including antibiotics, ventilator support, percutaneous cardiopulmonary support, and continuous renal replacement therapy.

서울의 Penicillinase Producing Neisseria Gonorrhoeae 발생빈도(1996)

김재홍,황동규,전재홍,김윤석,김중환,김용준,이창균,임동진,김현수,조창근,김경문,박상훈,전우형,김희성,이호정,차명수,김갑형,김형석,김석우,황지환,박병순,권오상,이민수,송기훈,성소영,이인섭,부태성 대한화학요법학회 1999 대한화학요법학회지 Vol.17 No.2

Background : In recent years, gonorrhea has been panedemic and remains one of the most commom STDs in the world, especially in developing countries. Objective & Methods: For the detection of a more effective therapeutic regimen and assessing the prevalence of PPNG, we have been trying to study the patients who have visited the VD Clinic of Choong-Ku Public Health Center in Seoul since 1980 by means of the chromogenic cephalosporin method. Results: In 1996, 139 strains of N. gonorrhoeae were isolated, among which 53(39.0%) were PPNG. Conclusion: Our results suggests that after a peak of 74.3% in 1993, the prevalence of PPNG in Seoul is gradually declining.

HPLC-MS/MS를 이용하여 혈장 중 레보티인정의 정량분석

채석,오형근,이상선,김형기,손동렬,염윤기,맹주양,권준택 순천향의학연구소 2008 Journal of Soonchunhyang Medical Science Vol.14 No.2

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가로변 건축물 외관에 있어서 창의 이미지 특성에 관한 연구

이청웅,양동협,강형구,최환석 朝鮮大學校 建設技術硏究所 2000 建設技術硏究 Vol.20 No.1

The window in architecture has a mysterious role connecting the indoor and outdoor world and also an interaction. this study investigates the functions of the past and present windows and its functions which will be required in future and aims a t seeking a planning direction of desirable window by integrating the understanding of window plan in outdoor space of building and new design techniques. The origin of window and its developmental process are expressed primarily by internal functions and it has been discussed from the external viewpoint with the development of structure, materials and social and cultural aesthetic sense. Accordingly it describes the history and place of windows to define the functions of windows and Chapter 2 states the forms, functions and treatments of window opening part according to the times. Chapter 3 cites the cases of the prototypes of forms shown in the outside of building as the functional and design elements windows in building. Chapter 4 suggests the positive cases of window prototype which produces the outdoor space of modern architecture and examines the correlation of it to the various elements according to the objective methods and order on the basis of SD method. Consequently, the windows in building should be planned by considering the following: 1. The window must be faithful to its original purposes. 2. It should be based on a great design because it is an important part which shows the appearance of building in urban area. 3. As today's buildings are large-scaled and high storied and also have unique characteristics, the development of construction technique and the production of good materials and products should be made. 4. The various general factors of windows should be analyzed and considered in planning windows as well as its design and technical sides.

2차음원 방향별 Modified-FxLMS 알고리즘의 소음감소 효과

이형석 주성전문대학 2008 論文集 Vol.17 No.-

Acoustic noise problems became more and more evident as increased numbers of large industnal equipment such as engines, blower, fans and compressors are in use. In an effort to overcome these problems, active noise control(ANC), in which additional secondary sources are used to cancel noise from the original primary source, has received considerable interest and has shown significant promise. In this pacer, we studied on an attenuation effect of automobile exhaust noise according to the direction of secondary sound source in ANC system. Automobile exhaust noise was recorded at 800rpm, 3500rpm and 5000rpm of a diesel engine Directions of loudspeaker(second sound source) can be set to 0˚, 30˚, 90˚ and 150˚ against the primary noise flow by acrylic ducts to be made for the experimentation DSP board with TMS320C6416 chip of Texas Instrument Co used to control the adaotive ANC system. This ANC system is based on the single-channel FxLMS algorithm that is necessary to compensate for the secondary path transfer function.

강구조 복앵글 전단 접합부의 구조적 거동에 관한 실험적 연구

이도형,김석중 江原大學校 産業技術硏究所 1997 産業技術硏究 Vol.17 No.-

Shear connection in steel structures should satisfy dual criteria of shear strength and retational flexibility and ductility. The connection should be strong enough to transfer the shear reaction of the beam, and should have sufficient rotational flexibility and ductility to rotate easily and supply the end rotation demand of the beam. This paper is concerned with the behavior of double-angle shear connections where the parameters are numbers of high strength bolt pitch, the length of angle leg, and connection method. An experimental investigation of shear connection was conducted by testing 12 beam-to-column joint specimens. Based on experimental and analytical study, the failure modes are developed and proposed design formulas.

백당으로 가교된 폴리아크릴산 하이드로겔의 In vitro 점막부착력 평가

이재휘,김선영,이은석,이민석,김형수,최영욱 한국약제학회 2006 Journal of Pharmaceutical Investigation Vol.36 No.2

전립선암의 종양지표로서 전립선특이항원의 진단적 가치

이남규,박영호,최석형 순천향대학교 1994 논문집 Vol.17 No.4

Prostatic specipic antigen(PSA), neutral serine protease secreted exclusively by prostatic epithelial cell, has a number of application in the diagnosis of men with prostatic carcinoma. It is widely recognized that elevated PSA correlates with the presence of carcinoma. We measured serum PSA levels in normal controls, patients with benign prostate hyperplasia symptoms because to evaluate diagnostic value of PSA as a tumor marker for prostatic cancer. Mean values of serum PSA in normal controls and patients with BPH symptoms were 2.83±4.02, 23.48±62.17ng/ml, respectively(p<0.01). We conclude that serum PSA offers simple and objective tumor maker but interpretation of significance of a specific level measured is inexact.