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Lee, Seungeun,Lee, Eunhye,Ko, EunYi,Ham, Mina,Lee, Hye Min,Kim, Eun-Sook,Koh, Minsoo,Lim, Hyun Kyung,Jung, Joohee,Park, So Yeon,Moon, Aree Elsevier 2018 Cancer letters Vol.437 No.-
<P><B>Abstract</B></P> <P>Tumor-associated macrophages (TAMs) are major components of tumor microenvironment that promote invasion and metastasis of cancer cells. In this study, we investigated the effect of TAMs on phenotypic conversion of non-neoplastic MCF10A human breast epithelial cells using an indirect co-culture system. Co-culture with TAMs induced epithelial-to-mesenchymal transition, invasive phenotype, and MMP-9 upregulation in MCF10A cells. Comparative proteomic analysis revealed that endoplasmic reticulum oxidoreductase (ERO)1-α was increased in MCF10A cells co-cultured with TAMs compared to that in mono-cultured cells. ERO1-α was crucial for TAMs-induced invasive phenotype and MMP-9 upregulation involving transcription factors c-fos and c-Jun. Cytokine array analysis showed that levels of interleukin (IL)-6, C-X-C motif ligand (CXCL)1, C-C motif ligand (CCL)2, growth-regulated protein (GRO), IL-8, and granulocyte-macrophage colony-stimulating factor (GM-CSF) were increased in conditioned media of co-cultured cells. Among these cytokines increased in conditioned media of co-cultured cells, CCL2 was secreted from TAMs, leading to induction of ERO1-α, MMP-9 upregulation, and invasiveness in MCF10A cells. Our findings elucidated a molecular mechanism underlying the aggressive phenotypic change of non-neoplastic breast cells by co-culture with TAMs, providing useful information for prevention or treatment of recurrent breast cancer.</P> <P><B>Highlights</B></P> <P> <UL> <LI> TAMs induced invasion in MCF10A cells non-neoplastic human breast epithelial cells. </LI> <LI> ERO1-α was increased in MCF10A cells co-cultured with TAM. </LI> <LI> ERO1-α was crucial for the TAMs-induced invasive phenotype and MMP-9 upregulation. </LI> <LI> CCL2 was secreted from TAMs co-cultured with MCF10A cells. </LI> <LI> CCL2 induced ERO1-α, resulting MMP-9 upregulation and invasiveness in MCF10A cells. </LI> </UL> </P>
Lee, Jonghwa,Kim, Leesun,Shin, Yongho,Lee, Junghak,Lee, Jiho,Kim, Eunhye,Moon, Joon-Kwan,Kim, Jeong-Han American Chemical Society 2017 Journal of agricultural and food chemistry Vol.65 No.16
<P>A multiresidue method for the simultaneous and rapid analysis of 360 pesticides in representative agricultural produce (brown rice, orange, spinach, and potato) was developed using a modified QuEChERS procedure combined with gas chromatography tandem mass spectrometry (GC-MS/MS). Selected reaction monitoring transition parameters (e.g., collision energy, precursor and product ions) in MS/MS were optimized to achieve the best selectivity and sensitivity for a wide range of GC-amenable pesticides. A short (20 m) microbore (0.18 mm i.d.) column resulted in better signal-to-noise ratio with reduced analysis time than a conventional narrowbore column (30 m X 0.25 mm i.d.). The priming injection dramatically increased peak areas by masking effect on a new GC liner. The limit of quantitation was <0.01 mg/kg, and the correlation coefficients (r(2)) of matrix-matched standards were >0.99 within the range of 0.0025-0.1 mg/kg. Acetonitrile with 0.1% formic acid without additional buffer salts was used for pesticide extraction, whereas only primary secondary amine (PSA) was used for dispersive solid phase extraction (dSPE) cleanup, to achieve good recoveries for most of the target analytes. The recoveries ranged from 70 to 120% with relative standard deviations of <= 20% at 0.01 and 0.05 mg/kg spiking levels (n = 6) in all samples, indicating acceptable accuracy and precision of the method. Seventeen real samples from local markets were analyzed by using the optimized method, and 14 pesticides in 11 incurred samples were found at below the maximum residue limits.</P>
Lee, Seongjin,Cha, Eunhye,Lee, Jongcheol,Lee, Jongdeok,Song, Inja,Kim, Sungchul KOREAN PHARMACOPUNCTURE INSTITUTE 2017 Journal of pharmacopuncture Vol.20 No.1
Objectives: Studies involving patients with spinobulbar muscular atrophy (SBMA), which is often referred to as Kennedy's disease, similar to those involving patients with progressive muscular disease (PMD), are rare. This paper reports a case study involving the use of Korean medicine to treat a patient with SBMA. Methods: We treated a patient with SBMA with unique symptoms by using various kinds of pharmacopuncture and herbal medicines for about two and a half years. After the treatment had ended, we evaluated the patient's conditions and the side effects of the treatment. Results: After treatment, the patient's symptoms were stabilized, and the patient suffered no abnormalities or side effects. No special changes in condition were noted during treatment period, and the patient was very satisfied with his response to treatment. Conclusion: Existing treatments have some considerable after effects and are difficult to apply in domestic clinics. In this regard, our findings should open possibilities for new clinical guidelines. Nevertheless, the limitations associated with this case study should be resolved, and more studies need to be conducted.
Eunhye Lee,Inkyeoung Kim 대한말초신경학회 2016 The Nerve Vol.2 No.2
Acute visual loss resulting from an uncomplicated cranioplasty is rare. A 52-year-old man presented with computed tomography (CT)-proven acute subdural hematoma in the right frontotemporoparietal area. The patients got a standard craniotomy in supine position. But on postoperative day 24, he was diagnosed postoperative epidural and subdural empyema. The patient underwent infected bone flap removal and evacuation of epidural and subdural empyema. Four months later, he got right frontotemporoparietal cranioplasty with artificial bone (Medpor) after controlling of infection. But on postoperative day 15, he complained of inability to see with his right eye. Brain and orbital CT, magnetic resonance imaging scan performed postoperatively did not show any new intracranial or intraorbital pathology. There were not any adverse events such as excessive pressure placed on the eyeball or bulky retraction of frontal skin flap. Visual evoked potential revealed prechiasmatic lesion. Fundoscopy showed slightly pallor optic disc of right side. The patient’s vision in the right eye remained no light perception permanently. The reason and pathophysiology of the mechanism of this complication should be discussed through reporting more cases.
Eunhye Kim,문준관,Hoon Choi,Su-Myoung Hong,Dong-Hyuk Lee,Hyomin Lee,김정한 한국응용생명화학회 2012 Applied Biological Chemistry (Appl Biol Chem) Vol.55 No.1
Exposure and risk assessments were conducted to evaluate safety of speed spayer (SS) and power sprayer (PS) used for treatment of insecticide methomyl in apple orchard on the operator. Dermal patches, gloves, socks, and masks were used to monitor the potential dermal exposure, and personal air monitor with XAD-2 resins was used to evaluate the potential inhalation exposure. Validation of methods for limit of detection, limit of quantitation, recovery, reproducibility, linearity of calibration, trapping efficiency, and breakthrough tests were performed to obtain reasonable results for quantitative exposure study of methomyl. During application of methomyl, PS resulted in more dermal exposure than SS. Important contaminated parts of body were upper arms, thigh, chest, shin, hand, forearm, and head for both SS and PS. Exposure rate was 44–176 mL/h. Although the level of inhalation exposure was very low during application, relatively higher level was observed for PS than for SS. During mixing/loading, more dermal exposure occurred by SS than that of PS probably due to drift of wettable powder (WP) formulation. Exposure was mostly observed on hand, and 99.9% of hand exposure to soluble liquid formulation (215 mg) in PS was from spill of liquid formulation on gloves. However, the body exposure ratio to total mixing/loading amount and inhalation exposure during mixing/loading was very low. Margin of safety in risk assessment was much larger than 1 in all cases, indicating low risk.
Lee, Hyeri,Riu, Myoungjoo,Kim, Eunhye,Moon, Joon-Kwan,Choi, Hoon,Do, Jung-A,Oh, Jae-Ho,Kwon, Ki-Sung,Lee, Young Deuk,Kim, Jeong-Han 한국응용생명화학회 2013 Applied Biological Chemistry (Appl Biol Chem) Vol.56 No.2
A single residue analytical method was developed for herbicide chlorpropham in various crops. Brown rice, apple, mandarin, Kimchi cabbage, green pepper, potato, and soybean were selected as representative crops, and clean-up system, partition solvent, and extraction solvent were optimized. For high performance liquid chromatography (HPLC), $C_{18}$ column was used with elution solvents of water and acetonitrile. Limit of quantitation (LOQ) of chlorpropham was 2 ng (S/N >20), and excellent linearity ($R^2$=1.000) was achieved. Method limit of quantitation (MLOQ) was 0.02 mg/kg. For recovery tests, crop samples were macerated and fortified with chlorpropham standard solution at three fortification levels (MLOQ, 10 MLOQ, and 100 MLOQ). And then those were extracted with acetonitrile, concentrated and partitioned with n-hexane. The n-hexane layer was then concentrated, cleaned-up through Florisil$^{(R)}$ column with ethyl acetate:n-hexane (5:95, v/v) prior to concentration and analysis with HPLC. Good recoveries from 76.8 to 107.9% with coefficients of variation of less than 10% were obtained, regardless of sample type, which satisfies the criteria of Korea Food and Drug Administration. Those results were confirmed with liquid chromatography-mass spectrometry (LC-MS). The method established in this study could be applied to most of crops as an official and general method for the analysis of chlorpropham residue.
The Effects of Acupotomy Therapy on Carpal Tunnel Syndrome: A Report of 4 Cases
Lee, Seongjin,Cha, Eunhye,Yang, Muhak,Lee, Jongdeok,Lee, Jiyoung,Lee, Sojin,Kim, Deokho,Kim, Sungchul Korean AcupunctureMoxibustion Medicine Society 2018 Korean Journal of Acupuncture Vol.35 No.1
The aim of this report was to show the effects of acupotomy in patients with carpal tunnel syndrome. Four patients were treated with acupotomy twice. Visual analogue scale (VAS), Tinel's sign, Phalen's test, Boston carpal tunnel syndrome questionnaire (BCTQ), muscular strength test, and a cross-sectional area of median nerve was measured using ultrasound before and after treatment. In all 4 cases, the VAS score, BCTQ score and cross-sectional area of median nerve, all decreased and muscular strength test score increased. Tinel's sign and the Phalen's test changed from a positive to a negative in most cases. This report shows that acupotomy is an effective treatment for carpal tunnel syndrome. Further larger are needed to fully evaluate the beneficial effects of this treatment.