Purification and Characterization of Fibrinolytic Enzyme Produced by Bacillus subtilis K7 Isolated from Korean Traditional Soy Sauce

Doo Young Kim,Eun Tag Lee,Sang Dal Kim 한국응용생명화학회 2003 Journal of Applied Biological Chemistry (J. Appl. Vol.46 No.3

Molecular characterization of acidic peptide:<i>N</i>-glycanase from the dimorphic yeast <i>Yarrowia lipolytica</i>

Lee, Kyung Jin,Gil, Jin Young,Kim, Sang-Yoon,Kwon, Ohsuk,Ko, Kisung,Kim, Dong-Il,Kim, Dae Kyong,Kim, Ha Hyung,Oh, Doo-Byoung Japanese Biochemical Society 2015 The Journal of biochemistry Vol.157 No.1

<P>Peptide:<I>N</I>-glycanase (PNGase) A is used preferentially to cleave the glycans from plant and insect glycopeptides. Although many putative PNGase A homologous genes have been found in the plant and fungus kingdoms through sequence similarity analyses, only several PNGases from plants and one from a filamentous fungus have been characterized. In this study, we identified and characterized a PNGase A-like enzyme, PNGase Yl, in the dimorphic yeast <I>Yarrowia lipolytica</I>. The corresponding gene was cloned and recombinantly expressed in <I>Pichia pastoris</I>. The purified enzyme cleaved glycans from glycopeptides with the maximum activity at pH 5. No metal ions were required for full activity, and rather it was repressed by three metal ions (Fe<SUP>3+</SUP>, Cu<SUP>2+</SUP> and Zn<SUP>2+</SUP>). Using glycopeptide substrates, PNGase Yl was shown to release various types of <I>N</I>-glycans including high-mannose and complex-type glycans as well as glycans containing core-linked α(1,3)-fucose that are frequently found in plants and insects. Moreover, in comparison with PNGase A, PNGase Yl was able to cleave with higher efficiency the glycans from some denatured glycoproteins. Taken together, our results suggest that PNGase Yl, the first biochemically characterized yeast PNGase A homologue, can be developed through protein engineering as a useful deglycosylation tool for <I>N</I>-glycosylation study.</P>

Various Metallic Nano-Sized Patterns Fabricated Using an Ag Ink Printing Technique

Sang-Chul Oh,Ki-Yeon Yang,변경재,Ju-Hyeon Shin,Yang-Doo Kim,Lee-Mi Do,Kyung-Woo Choi,이헌 대한금속·재료학회 2012 ELECTRONIC MATERIALS LETTERS Vol.8 No.5

This paper presents a new simple metal patterning technique, which is based on soft nanoimprint lithography. By using this method with a commercial Ag nano particle ink, a nano-sized metal pattern was successfully fabricated. The problem of the residual layer of patterned Ag layer was minimized by controlling the concentration of the solution and the process conditions. By using this method, we could easily fabricate various patterns without reference to any shape. Furthermore, we fabricated an Ag mesh type pattern for the application of conducting transparent glass.

Transumbilical laparoscopic-assisted appendectomy is a useful surgical option for pediatric uncomplicated appendicitis

Doo Yeon Go,Yoon Jung Boo,Ji Sung Lee,Cheol Woong Jung 대한외과학회 2016 Annals of Surgical Treatment and Research(ASRT) Vol.91 No.2

Purpose: Transumbilical laparoscopic-assisted appendectomy (TULA) is a single incision technique that uses a combined intra- and extracorporeal method. The aim of this study was to compare surgical outcomes of TULA with conventional 3-port laparoscopic appendectomy (LA). Methods: A retrospective review of medical records between 2010 and 2014 identified 303 pediatric patients who underwent LA with uncomplicated acute appendicitis. Of these, 85 patients underwent TULA and 218 patients underwent conventional LA. Demographic data, clinical characteristics, perioperative outcomes and postoperative complications were compared between the 2 groups. Results: The mean operation time in the TULA group was 30.39 minutes, which was significantly shorter than that of the LA group (47.83 minutes) (P < 0.001). The first day of oral intake after surgery was earlier (1.05 days vs. 1.32 days; P < 0.001) and the length of hospital stay was also shorter (2.54 days vs. 3.22 days; P < 0.001) for the TULA group than the LA group. Furthermore, the postoperative complication rate was lower in the TULA group (1 of 85, 1.25%) compared to the LA group (19 of 218, 8.7%) (P = 0.018). Conclusion: In conclusion, TULA procedure is recommended for uncomplicated appendicitis in children due to its simplicity and better postoperative outcomes.

Phospholipase D2 drives mortality in sepsis by inhibiting neutrophil extracellular trap formation and down-regulating CXCR2

Lee, Sung Kyun,Kim, Sang Doo,Kook, Minsoo,Lee, Ha Young,Ghim, Jaewang,Choi, Youngwoo,Zabel, Brian A.,Ryu, Sung Ho,Bae, Yoe-Sik The Rockefeller University Press 2015 The Journal of experimental medicine Vol.212 No.9

<P>Lee et al. find that phospholipase D2 deficiency increases survival and decreases organ damage during experimental sepsis in mice which could be reversed with a CXCR2 antagonist. Thus, targeting PLD2 may offer therapeutics for septic patients.</P><P>We determined the function of phospholipase D2 (PLD2) in host defense in highly lethal mouse models of sepsis using PLD2<SUP>−/−</SUP> mice and a PLD2-specific inhibitor. PLD2 deficiency not only increases survival but also decreases vital organ damage during experimental sepsis. Production of several inflammatory cytokines (TNF, IL-1β, IL-17, and IL-23) and the chemokine CXCL1, as well as cellular apoptosis in immune tissues, kidney, and liver, are markedly decreased in PLD2<SUP>−/−</SUP> mice. Bactericidal activity is significantly increased in PLD2<SUP>−/−</SUP> mice, which is mediated by increased neutrophil extracellular trap formation and citrullination of histone 3 through peptidylarginine deiminase activation. Recruitment of neutrophils to the lung is markedly increased in PLD2<SUP>−/−</SUP> mice. Furthermore, LPS-induced induction of G protein–coupled receptor kinase 2 (GRK2) and down-regulation of CXCR2 are markedly attenuated in PLD2<SUP>−/−</SUP> mice. A CXCR2-selective antagonist abolishes the protection conferred by PLD2 deficiency during experimental sepsis, suggesting that enhanced CXCR2 expression, likely driven by GRK2 down-regulation in neutrophils, promotes survival in PLD2<SUP>−/−</SUP> mice. Furthermore, adoptively transferred PLD2<SUP>−/−</SUP> neutrophils significantly protect WT recipients against sepsis-induced death compared with transferred WT neutrophils. We suggest that PLD2 in neutrophils is essential for the pathogenesis of experimental sepsis and that pharmaceutical agents that target PLD2 may prove beneficial for septic patients.</P>

Copper–Nickel Alloy Plating to Improve the Contact Resistivity of Metal Grid on Silicon Heterojunction Solar Cells

Sang Hee Lee,Doo Won Lee,Kyoung-jin Lim,Won-suk Shin,Jeong Kim 대한금속·재료학회 2019 ELECTRONIC MATERIALS LETTERS Vol.15 No.3

As a dominant metallization technique of crystalline silicon solar cells, screen printing with silver paste has been generallyused in photovoltaic industries. In case of the silicon heterojunction solar cells (SHJ) structure, a metal contact with silverpaste has lower electrical conductivity than pure silver due to the other compositions of the paste. For the reason, copperplating is attractive substitute for the silver paste since the plated-copper contacts have high conductivity and easily reduceline width which is benefi cial to light absorption. In this experiment, we studied copper–nickel (Cu–Ni) alloy plating to forma seed layer of the copper plating on an indium tin oxide (ITO) layer which is used for the transparent conductive oxide ofthe SHJ solar cells. As a requirement of suitable seed layer, contact resistivity (ρ c ) between the seed and the ITO is importantto obtain high fi ll factor by decreasing series resistance of solar cells. Contact resistivity values of the samples with variednickel contents in the Cu–Ni fi lms were extracted by using transfer length method. Also, the composition ratio of the alloylayer was analyzed by energy dispersive spectrometer. Moreover, X-ray diff raction was used to compare lattice parameterand crystallite size of the fi lm.

pH-Triggered Targeting Polymeric Nanocarriers: Theranostic Applications

Doo Sung Lee 한국고분자학회 2016 한국고분자학회 학술대회 연구논문 초록집 Vol.41 No.2

Analysis of Radioactivity in Coal Fly Ash

Lee,Myung-Ho,Kim,Mi-Kyung,Shin,Hyun-Sang,Park,Doo-Wun,Lee,Chang-Woo,Rhee,Dong-Seok 대한방사선 방어학회 2000 방사선방어학회지 Vol.24 No.4

국내 석탄화력발전소에서 발생되는 비산석탄회의 방사능 농도 분석을 수행하였고 재활용을 위한 방사능 함량의 유해도를 평가하였다. TBP 용매추출법과 알파스펙트로메타를 사용하여 비산석탄회에 존재하는 우라늄 동위원소(U, U, U)의 방사능 농도를 측정하였으며, 감마스펙트로메타를 사용하여 Ra, Th, K 등의 방사능 농도를 결정하였다. 우라늄 동위원소의 농도측정결과 U, U, U의 방사능 농도은 평균적으로 각각 116.1, 5.01, 및 121.2 ㏃ ㎏으로 나타났으며, U/U의 방사능 농도비는 1.04 ± 0.03 로서 자연상태의 토양중 방사능 농도 비인 1.14 와 유사하였다. 동일시료에 대한 감마측정 결과 Ra, Th 및 K의 방사능 농도는 각각 101.7 ∼ 113.9, 39.5 ∼ 54.2 및 315.0 ∼ 990.6 ㏃ ㎏ 으로 나타났다. Ra, Th 및 K 동위원소의 농도를 사용하여 재활용 목적으로서의 비산석탄회의 방사능 함량 유해도를 평가하였고, 그 결과 본 연구의 대상물질인 비산석탄회의 방사능 함량은 허용기준치 이하로 나타났다. The specific radioactivity concentrations in the coal fly ash obtained from heat producing stations in Korea were analyzed and its radiological hazard for reuse in construction purpose was evaluated. The concentrations of uranium isotopes in the coal fly ash measured by TBP solvent extraction method and α-spectrometer were found to be about 116.1 ㏃ ㎏ for U, and 121.2 ㏃ ㎏ for U, respectively. The actibity ratio of U/U, in the coal fly ash was in 1.04 ± 0.03, which is similar to that of uncontaminated Korean soil in natural conditions (1.14). The specific radioactivities of Ra, Th and K in the coal fly ash were also determined using γ-spectrometer with a HPGe detector. The results showed that Ra, Th, and k in the coal fly ash were in concentrations of 101.7 ∼ 113.9, 39.5 ∼ 54.2 and 315.0 ∼ 990.6 ㏃ ㎏, respectively. With the specific radioactivities obtained from γ-spectrometric measurements of the coal fly ash, its radiological hazard for reuse was evaluated. The result showed that the radioactivity of the coal fly ash was in permissible level.

Review : The Reverse Proteomics for Identification of Tumor Antigens

( Sang Yull Lee ),( Doo Il Jeoung ) 한국미생물 · 생명공학회 2007 Journal of microbiology and biotechnology Vol.17 No.6

Transcervical Embryo Recovery in Korean Black Goats: A Preliminary Experiment

Lee, Doo-Soo,Kim, Dong-Woo,Shin, Sang Tae The Korean Society of Embryo Transfer 2015 한국동물생명공학회지 Vol.30 No.3

Four pluriparous Korean black goat does were superovulated with FSH and mated with fertile bucks. Anesthetized animals were placed in lateral recumbency, then size 8 Foley catheter was inserted into the uterus through the cervix under the vaginal speculum and the balloon was inflated to fix the catheter in the uterine body. The opposite end of the catheter was connected to a 3-way and a flushing medium was infused into the uterus. Modified Dubecco's PBS with 1% FBS was used as the flushing medium. Four goats were allocated in two groups depending on the type of medium infusion into uterus. Injection group; the flushing medium was injected into uterus and the infused medium was collected by to-and-fro method using a syringe. Gravity-flow group; the flushing medium was allowed to enter the uterus by gravity flow by lifting the medium bottle and drained out of the uterus into a collecting tube. All four goats had catheter inserted through the cervix and uteri flushed successfully. The volume (recovery rate) of recovered medium varied considerably from 87 ml/200 ml (43.5%) to 148 ml/160 ml (92.5%). Nine embryos/ova in total were recovered from Gravity-flow group goats. Although the embryo recovery rate was low, the possibility of a transcervical embryo recovery in Korean black goat had been proven in this preliminary experiment.