Re-identification of Colletotrichum gloeosporioides Species Complex Isolates in Korea and Their Host Plants

Le Dinh Thao,Hyorim Choi,Yunhee Choi,Anbazhagan Mageswari,Daseul Lee,Dong-Hyun Kim,Shin Hyeon-Dong,Choi Hyo-Won,주호종,Hong Seung-Beom 한국식물병리학회 2024 Plant Pathology Journal Vol.40 No.1

The Colletotrichum gloeosporioides species complex includes many phytopathogenic species, causing anthracnose disease on a wide range of host plants and appearing to be globally distributed. Seventy-one Colletotrichum isolates in the complex from different plants and geographic regions in Korea were preserved in the Korean Agricultural Culture Collection (KACC). Most of them had been identified based on hosts and morphological features, this could lead to inaccurate species names. Therefore, the KACC isolates were re-identified using DNA sequence analyses of six loci, comprising internal transcribed spacer, gapdh, chs-1, his3, act, and tub2 in this study. Based on the combined phylogenetic analysis, KACC strains were assigned to 12 known species and three new species candidates. The detected species are C. siamense (n = 20), C. fructicola (n = 19), C. gloeosporioides (n = 9), C. aenigma (n = 5), C. camelliae (n = 3), C. temperatum (n = 3), C. musae (n = 2), C. theobromicola (n = 2), C. viniferum (n = 2), C. alatae (n = 1), C. jiangxiense (n = 1), and C. yulongense (n = 1). Of these, C. jiangxiense, C. temperatum, C. theobromicola and C. yulongense are unrecorded species in Korea. Host plant comparisons showed that 27 fungus-host associations are newly reported in the country. However, plant-fungus interactions need to be investigated by pathogenicity tests.

Re-identification of Colletotrichum acutatum Species Complex in Korea and Their Host Plants

Le Dinh Thao,Hyorim Choi,Yunhee Choi,Anbazhagan Mageswari,Daseul Lee,Seung-Beom Hong 한국식물병리학회 2023 Plant Pathology Journal Vol.39 No.4

Colletotrichum acutatum species complex is one of the most important groups in the genus Colletotrichum with a high species diversity and a wide range of host plants. C. acutatum and related species have been collected from different plants and locations in Korea and deposited into the Korean Agricultural Culture Collection (KACC), National Institute of Agricultural Sciences since the 1990s. These fungal isolates were previously identified based mainly on morphological characteristics, and a limitation of molecular data was provided. To confirm the identification of species, 64 C. acutatum species complex isolates in KACC were used in this study for DNA sequence analyses of six loci: nuclear ribosomal internal transcribed spacers (ITS), betatubulin 2 (TUB2), histone-3 (HIS3), glyceraldehyde- 3-phosphate dehydrogenase (GAPDH), chitin synthase 1 (CHS-1), and actin (ACT). The molecular analysis revealed that they were identified in six different species of C. fioriniae (24 isolates), C. nymphaeae (21 isolates), C. scovillei (12 isolates), C. chrysanthemi (three isolates), C. lupini (two isolates), and C. godetiae (one isolate), and a novel species candidate. We compared the hosts of KACC isolates with “The List of Plant Diseases in Korea”, previous reports in Korea and global reports and found that 23 combinations between hosts and pathogens could be newly reported in Korea after pathogenicity tests, and 12 of these have not been recorded in the world.

Results-oriented Culture, Performance Information Use and the Performance of Public Organizations: Evidence from Vietnam

Thao Hong Phuong CHAU,Truc Dinh LE,Quynh Xuan PHAM 한국유통과학회 2021 The Journal of Asian Finance, Economics and Busine Vol.8 No.8

The purpose of this research is to investigate the mediating role of performance information use in the relationship between result-oriented culture and organizational performance. The data were collected from chief accountants and managers working in public-sector organizations in Vietnam. The analysis was based on the 197 completed questionnaires by using Partial Least Squares Structural Equation Modeling (PLSSEM). For the test research model and hypotheses, the supporting tool is SmartPLS 3 software. Research findings show that result-oriented culture is positively associated with organizational performance, and that performance information use is a mediating mechanism that explains how a result-oriented culture leads to higher performance in public organizations. The findings of this study suggest that the resultsoriented culture, not only directly improves organizational performance, but also indirectly does so by the use of performance information. This study provides evidence for theoretical understanding of the impact of result-oriented culture on the performance of public-sector organizations through performance information use, and suggests practical implications for public organizations in applying result-oriented culture to foster using performance measurement and improve organizational performance. In order to achieve good performance, publicsector organizations need to create and spread result-oriented culture and enhance the use of performance information for decision-making.

BONEcheck: A digital tool for personalized bone health assessment

Dinh Tan Nguyen,Thao P. Ho-Le,Liem Pham,Vinh P. Ho-Van,Tien Dat Hoang,Thach S. Tran,Steve Frost,Tuan V. Nguyen 대한골다공증학회 2023 Osteoporosis and Sarcopenia Vol.9 No.3

Objectives: Osteoporotic fracture is a significant public health burden associated with increased mortality risk and substantial healthcare costs. Accurate and early identification of high-risk individuals and mitigation of their risks is a core part of the treatment and prevention of fractures. Here we introduce a digital tool called 'BONEcheck' for personalized assessment of bone health. Methods: The development of BONEcheck primarily utilized data from the prospective population-based Dubbo Osteoporosis Epidemiology Study and the Danish Nationwide Registry. BONEcheck has 3 modules: input data, risk estimates, and risk context. Input variables include age, gender, prior fracture, fall incidence, bone mineral density (BMD), comorbidities, and genetic variants associated with BMD. Results: Based on the input variables, BONEcheck estimates the probability of any fragility fracture and hip fracture within 5 years, subsequent fracture risk, skeletal age, and time to reach osteoporosis. The probability of fracture is shown in both numeric and human icon array formats. The risk is also contextualized within the framework of treatment and management options on Australian guidelines, with consideration given to the potential fracture risk reduction and survival benefits. Skeletal age was estimated as the sum of chronological age and years of life lost due to a fracture or exposure to risk factors that elevate mortality risk. Conclusions: BONEcheck is an innovative tool that empowers doctors and patients to engage in wellinformed discussions and make decisions based on the patient's risk profile. Public access to BONEcheck is available via https://bonecheck.org and in Apple Store (iOS) and Google Play (Android).

Re-Identification of Aspergillus Subgenus Circumdati Strains in Korea Led to the Discovery of Three Unrecorded Species

Anbazhagan Mageswari,Yunhee Choi,Le Dinh Thao,Daseul Lee,Dong-Hyun Kim,Myung Soo Park,홍승범 한국균학회 2023 Mycobiology Vol.51 No.5

Aspergillus is one of the largest and diverse genera of fungi with huge economical, biotechnological,and social significance. Taxonomically, Aspergillus is divided into six subgeneracomprising 27 sections. In this study, 235 strains of Aspergillus subgenus Circumdati (section:Candidi, Circumdati, Flavi, Flavipedes, Nigri, and Terrei) preserved at the Korean AgriculturalCulture Collection (KACC) were analyzed and re-identified using a combined dataset of partialb-tubulin (BenA), Calmodulin (CaM) gene sequences and morphological data. We confirmednineteen species to be priorly reported in Korea (A. neotritici, A. terreus, A. floccosus,A. allahabadii, A. steynii, A. westerdijkiae, A. ochraceus, A. ostianus, A. sclerotiorum, A. luchuensis,A. tubingensis, A. niger, A. welwitschiae, A. japonicus, A. nomius, A. tamarii, A. parasiticus, A. flavi, and A. oryzae). Among the studied strains, three species (A. subalbidus, A. iizukae, andA. uvarum), previously unreported or not officially documented, were discovered in Korea, tothe best of our knowledge. We have given a detailed description of the characteristic featuresof the three species, which remain uncharted in Korea.