新時期中國古典文學硏究的回顧

劉躍進 順天鄕大學校 人文科學硏究所 1998 순천향 인문과학논총 Vol.6 No.-

Coupling effects of nitrogen and phosphorus on photosynthesis and chlorophyll fluorescence parameters of Cyclocarya paliurus

Xiliang Yue,Xiaofang Liu1,Shengzuo Fang 강원대학교 산림과학연구소 2022 강원대학교 산림과학연구소 학술대회 Vol.2022 No.10

Cyclocarya paliurus has traditionally been used as medicine or nutraceutical foods in China, which has great value for market prospects. In order to determine the effects of nitrogen and phosphorus coupling on photosynthesis, chlorophyll (Chl) fluorescence and chlorophyll level of C. paliurus, a hydroponic experiment was laid out in a completely randomized block design with two-way factorial was used for C. paliurus cultivars with three N and three P treatments replicated three timesnin a greenhouse. The factorial combination of three N concentrations (50(low), 150(medium) and 250(high) mg/L) and three P concentrations (5(low), 15(medium), and 25(high) mg/L), totally nine treatments. Photosynthetic parameters (Pn, Ci, Gs and Tr), ChlF parameters (F0, Fm, Fv/Fm, Fv, PSⅡ, ETR, NPQ and qP) and chlorophyll level (SPAD) of leaves were measured at 30 and 60 days. The results demonstrated that the coupling of nitrogen and phosphorus had an interaction on photosynthesis, chlorophyll fluorescence and relative chlorophyll content of C. paliurus leaves. The photosynthetic rate, intercellular carbon dioxide concentration and stomatal conductance were the highest in N2P3 treatment (30 or 60 day), and the lowest were in N1P1 treatment. On the 60th day, F0, Fm, Fv were the lowest in N2P1 treatment, ФPSII and qp were higher in N2P3 treatment, NPQ was the highest in N3P1 treatment, Fv/Fm and ETR reached the maximum in N2P2 and N3P1 treatment respectively. The relative chlorophyll content reached the maximum in N2P2 treatment. Additionally, the correlation analysis results showed that the Pn was significantly positively correlated with Fv/Fm PhiPS2 Ci, Gs, Tr and SPAD value, and was significantly negatively correlated with F0 and NPQ, Fv, PhiPS2, qp was positively correlated with SPAD value. Thus, photosynthetic parameters, ChlF parameters and chlorophyll level measurements provide valuable physiological data. N2P2 or N2P3 treatment was more beneficial to the growth of C. paliurus.

Knowledge Acquisition Approach Based on Rough Set in Online Aided Decision System for Food Processing Quality and Safety

Liu Peng,Liu Wen,Li Qiang,Yang Li,Duan Min,Dai Yue 보안공학연구지원센터 2014 International Journal of u- and e- Service, Scienc Vol.7 No.6

For the problem that the use effect of food processing information system is poor due to absence of knowledge acquisition measures and knowledge self-updating function, a knowledge acquisition approach based on rough set is put forward. First, the approach establishes a set of predicted samples for the relationship between food processing parameters and product quality; then uses the discretization of continuous attribute, attribute reduction and rule extraction algorithm of rough set to acquire automatically predicted knowledge from a large number of predicted sample sets, and then saves the predicted knowledge in the knowledge base of expert system; finally, realizes the extraction of knowledge of food processing process based on the inference engine, which greatly enhances the effectiveness and practicality of the acquired knowledge in online aided decision system of the food processing quality and safety.

Influence of Test Methods on Wi-Fi Fingerprint-Based Indoor Positioning System

Yue Liu,Yiqiao Wei,Rashmi Sharan Sinha,Seung-Hoon Hwang 한국통신학회 2019 한국통신학회 학술대회논문집 Vol.2019 No.1

Short Low Concentration Cisplatin Treatment Leads to an Epithelial Mesenchymal Transition-like Response in DU145 Prostate Cancer Cells

Liu, Yi-Qing,Zhang, Guo-An,Zhang, Bing-Chang,Wang, Yong,Liu, Zheng,Jiao, Yu-Lian,Liu, Ning,Zhao, Yue-Ran Asian Pacific Journal of Cancer Prevention 2015 Asian Pacific journal of cancer prevention Vol.16 No.3

Background: Prostate cancer is one of the main causes of cancer death, and drug resistance is the leading reason for therapy failure. However, how this occurs is largely unknown. We therrfore aimed to study the response of DU145 cells to cisplatin. Materials and Methods: Du145 prostate cancer cells were treated with a low dose of cisplatin for 24 h and cell viability and number were determined by MTT assay and trypan blue exclusion assay, respectively. The real time polymerase chain reaction (PCR) was used to assess responses to cisplatin treatment. Results: After 24h $2{\mu}g/ml$ treatment did not result in significant reduction in cell viability or number. However, it led to enhanced cancer cell invasiveness. E-cadherin mRNA was reduced, and vimentin, Snail, Slug, metalloproteinase 9 (MMP9) mRNA expression increased significantly, a feature of epithelial-mesenchymal transition (EMT). Conclusions: Short time low concentration cisplatin treatment leads to elevated invasiveness of DU145 cancer cells and this is possibly due to EMT.

A decade of liver organoids: Advances in disease modeling

Yue Liu,Jian-Ying Sheng,Chun-Fang Yang,Junjun Ding,Yun-Shen Chan 대한간학회 2023 Clinical and Molecular Hepatology(대한간학회지) Vol.29 No.3

Liver organoids are three-dimensional cellular tissue models in which cells interact to form unique structures in culture. During the past 10 years, liver organoids with various cellular compositions, structural features, and functional properties have been described. Methods to create these advanced human cell models range from simple tissue culture techniques to complex bioengineering approaches. Liver organoid culture platforms have been used in various research fields, from modeling liver diseases to regenerative therapy. This review discusses how liver organoids are used to model disease, including hereditary liver diseases, primary liver cancer, viral hepatitis, and nonalcoholic fatty liver disease. Specifically, we focus on studies that used either of two widely adopted approaches: differentiation from pluripotent stem cells or epithelial organoids cultured from patient tissues. These approaches have enabled the generation of advanced human liver models and, more importantly, the establishment of patient-tailored models for evaluating disease phenotypes and therapeutic responses at the individual level.

Comparative Transcriptomics Identifies Different Gene Expression Networks Regulating Gametic Development in Arabidopsis

Yue Liu,Zhaoran Tian,Xiaofei Zhang,Zhengqing Xie,Baoming Tian,Weiwei Chen,Jialin Guo,Xiaochun Wei,Fang Wei,Gongyao Shi 한국식물학회 2023 Journal of Plant Biology Vol.66 No.6

Gamete development is a complex process during the plant reproductive life cycle. A global vision of the molecular mechanisms regulating gametic development would enhance our understanding of developmental similarities and differences between male and female reproductive pathways in plants. In this study, we applied comparative transcriptomics to characterize gene expression during sporogenesis and gametogenesis in Arabidopsis. Compared with genes expressed in leaves, 11,112 and 10,133 differentially expressed genes (DEGs) were identified in the anthers and ovules, respectively. The shared DEGs exhibited a similar expression pattern regarding cell cycle and chromatin dynamics, but DEGs associated with cell wall formation and pectin modifications were prevalent during male development, reflecting dynamic changes during pollen wall development in anthers. In contrast, DEGs related to DNA repair, homologous recombination, and RNA-directed DNA methylation (RdDM) were comparatively over-represented in ovules, probably indicating a relatively intensified mechanism for supervising genome fidelity during female gametogenesis. In addition, DEGs related to brassinolide (BR) synthesis and signaling pathway were also highly enriched in ovules, suggesting a pivotal role in female gamete development. Collectively, our results provide a contrasting transcriptional atlas in developing anthers and ovules, and shed new light on our global understanding of gamete development in plants.

Family Hilarimorphidae (Diptera) newly recorded from China with descriptions of three new species

Liu Yue,Gao Shang,Yang Ding 한국응용곤충학회 2023 Journal of Asia-Pacific Entomology Vol.26 No.2

The family Hilarimorphidae is reported from China for the first time. Three new species are described and illustrated: Hilarimorpha dingae sp. nov., Hilarimorpha elongata sp. nov. and Hilarimorpha tibetensis sp. nov. A key to species of Hilarimorpha from Asia and a checklist of world species of Hilarimorphidae are provided.

Survey on Visible Light-based Localization

Yue Liu,Shu-zhi Liu,Seung-Hoon Hwang 한국통신학회 2019 한국통신학회 학술대회논문집 Vol.2019 No.6

Indoor positioning technology based on visible light communication (VCL) combines lighting with communication. Compared with the traditional indoor wireless positioning method, the VCL-based indoor positioning technology has the advantages of green, energy saving, environmental protection, low cost, high precision and wide application, and has broad application prospects. We summarize the principles and features of two VLC-based indoor positioning technologies based on photodetectors and image sensors. We focus on the main problems in VLC-based indoor positioning technology, such as unreasonable layout of light sources, intersymbol interference and low sensitivity and stability of receiving equipment. In addition, We have summarized the relevant solutions. Finally, the future development trend and application prospects of indoor positioning technology based on VLC are discussed.

Senescence as A Consequence of Ginsenoside Rg₁ Response on K562 Human Leukemia Cell Line

Liu, Jun,Cai, Shi-Zhong,Zhou, Yue,Zhang, Xian-Ping,Liu, Dian-Feng,Jiang, Rong,Wang, Ya-Ping Asian Pacific Journal of Cancer Prevention 2012 Asian Pacific journal of cancer prevention Vol.13 No.12

Aims and Background: Traditional chemotherapy strategies for human leukemia commonly use drugs based on cytotoxicity to eradicate cancer cells. One predicament is that substantial damage to normal tissues is likely to occur in the course of standard treatments. Obviously, it is urgent to explore therapies that can effectively eliminate malignant cells without affecting normal cells. Our previous studies indicated that ginsenoside $Rg_1$ ($Rg_1$), a major active pharmacological ingredient of ginseng, could delay normal hematopoietic stem cell senescence. However, whether $Rg_1$ can induce cancer cell senescence is still unclear. Methods: In the current study, human leukemia K562 cells were subjected to $Rg_1$ exposure. The optimal drug concentration and duration with K562 cells was obtained by MTT colorimetric test. Effects of $Rg_1$ on cell cycle were analyzed using flow cytometry and by SA-${\beta}$-Gal staining. Colony-forming ability was measured by colony-assay. Telomere lengths were assessed by Southern blotting and expression of senescence-associated proteins P21, P16 and RB by Western blotting. Ultrastructural morphology changes were observed by transmission electron microscopy. Results: K562 cells demonstrated a maximum proliferation inhibition rate with an $Rg_1$ concentration of $20{\mu}\;mol{\cdot}L^{-1}$ for 48h, the cells exhibiting dramatic morphological alterations including an enlarged and flat cellular morphology, larger mitochondria and increased number of lysosomes. Senescence associated-${\beta}$-galactosidase (SA-${\beta}$-Gal) activity was increased. K562 cells also had decreased ability for colony formation, and shortened telomere length as well as reduction of proliferating potential and arrestin $G_2$/M phase after $Rg_1$ interaction. The senescence associated proteins P21, P16 and RB were significantly up-regulated. Conclusion: Ginsenoside $Rg_1$ can induce a state of senescence in human leukemia K562 cells, which is associated with p21-Rb and p16-Rb pathways.