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- 검색키워드
- 저자 : LIU Tie-qiao (검색결과 2 건)
- 무료
- 기관 내 무료
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Li, Ning,Tie, Xiao-Jing,Liu, Pei-Jie,Zhang, Yan,Ren, Hong-Zheng,Gao, Xin,Xu, Zhi-Qiao Asian Pacific Journal of Cancer Prevention 2013 Asian Pacific journal of cancer prevention Vol.14 No.2
Objective: To study the effects of down-regulation of HDAC6 expression on proliferation, cell cycling and migration of esophageal squamous cell carcinoma (ESCC) cells and related molecular mechanisms. Methods: ESCC cell line EC9706 cells were randomly divided into untreated (with no transfection), control siRNA (transfected with control siRNA) and HDAC6 siRNA (transfected with HDAC6 small interfering RNA) groups. Effects of HDAC6 siRNA interference on expression of HDAC6 mRNA and protein in EC9706 cells were investigated by semi-quantitative RT-PCR, Western blotting and immunocytochemistry methods. Effects of down-regulation of HDAC6 expression on cell proliferation, cell cycle, and cell migration were studied using a CCK-8 kit, flow cytometry and Boyden chambers, respectively. Changes of mRNA and protein expression levels of cell cycle related factor (p21) and cell migration related factor (E-cadherin) were investigated by semi-quantitative RT-PCR and Western blotting methods. Results: After transfection of HDAC6 siRNA, the expression of HDAC6 mRNA and protein in EC9706 cells was significantly downregulated. In the HDAC6 siRNA group, cell proliferation was markedly inhibited, the percentage of cells in G0/G1 phase evidently increased and the percentage of cells in S phase decreased, and the number of migrating cells significantly and obviously decreased. The mRNA and protein expression levels of p21 and E-cadherin in the HDAC6 siRNA group were significantly higher than those in the untreated group and the control siRNA group, respectively. Conclusions: HDAC6 siRNA can effectively downregulate the expression of HDAC6 mRNA and protein in EC9706 cells. Down-regulation of HDAC6 expression can obviously inhibit cell proliferation, arrest cell cycling in the G0/G1 phase and reduce cell migration. The latter two functions may be closely related with the elevation of mRNA and protein expression of p21 and E-cadherin.
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Soft Error Susceptibility Analysis for Sequential Circuit Elements Based on EPPM
CAI Shuo,KUANG Ji-shun,LIU Tie-qiao,WANG Wei-zheng 대한전자공학회 2015 Journal of semiconductor technology and science Vol.15 No.2
Due to the reduction in device feature size, transient faults (soft errors) in logic circuits induced by radiations increase dramatically. Many researches have been done in modeling and analyzing the susceptibility of sequential circuit elements caused by soft errors. However, to the best knowledge of the authors, there is no work which has well considerated the feedback characteristics and the multiple clock cycles of sequential circuits. In this paper, we present a new method for evaluating the susceptibility of sequential circuit elements to soft errors. The proposed method uses four Error Propagation Probability Matrixs (EPPMs) to represent the error propagation probability of logic gates and flip-flops in current clock cycle. Based on the predefined matrix union operations, the susceptibility of circuit elements in multiple clock cycles can be evaluated. Experimental results on ISCAS’89 benchmark circuits show that our method is more accurate and efficient than previous methods.
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