Plant responses to nano and micro structured carbon allotropes: Water imbibition by maize seeds upon exposure to multiwalled carbon nanotubes and activated carbon

Dasgupta-Schubert, N.,Tiwari, D.K.,Francis, E. Reyes,Martinez Torres, P.,Villasenor Cendejas, L.M.,Lara Romero, J.,Villasenor Mora, C. Techno-Press 2017 Advances in nano research Vol.5 No.3

Multiwalled carbon-nanotubes (MWCNT) and micro-structured carbon, such as biochar or activated carbon (AC), have been seen to significantly increase the growth indices of certain plant species such as maize (Zea mays L.). Seed imbibition is the stage where environmental factors that affect water transport across the seed coat barrier, make a large impact. This work explores the effect on water imbibition by maize seeds when the aqueous environment surrounding the seed is diluted by small concentrations (10 and 20 mg/l) of pristine MWCNT (p-MWCNT), carboxylate functionalized MWCNT (COO-MWCNT) and AC. The degree of sensitivity of the process to (i) large structural changes is seen by utilizing the nano (the MWCNT) and the micro (the AC) allotropic forms of carbon; (ii) to small changes in the purity and morphology of the p-MWCNT by utilizing 95% pure and 99% pure p-MWCNTs of slightly differing morphologies; and (iii) to MWCNT functionalization by using highly pure (97%) COO-MWCNT. Water imbibition was monitored over a 15 hour period by Near Infrared Thermography (NIRT) and also by seed weighing. Seed surface topography was seen by SEM imaging. Analysis of the NIRT images suggests rapid seed surface topological changes with the quantity of water imbibed. While further work is necessary to arrive at a conclusive answer, this work shows that the imbibition phase of the maize seed is sensitive to the presence of MWCNT even to small differences in the purity of the p-MWCNT and to small differences in the physicochemical properties of the medium caused by the hydrophilic COO-MWCNT.

Diagnosis of Benzimidazole Resistance in Haemonchus contortus of Sheep by Allele Specific PCR

Tiwari, J.,Kolte, A.P.,Kumar, S.,Swarnkar, C.P.,Singh, D.,Pathak, K.M.L. Asian Australasian Association of Animal Productio 2007 Animal Bioscience Vol.20 No.1

The study was conducted on 162 adult male Haemonchus contortus of sheep collected from Avikanagar, Jaipur and Bikaner regions to diagnose the benzimidazole (BZ) resistance in H. contortus. The BZ resistance is primarily linked with the mutation in ${\beta}$-tubulin isotype 1 gene which substitute phenylalanine (Phe) into tyrosine (Tyr) at the 200 codon of the gene. An allele specific polymerase chain reaction (AS-PCR) technique was used for diagnosis of BZ resistance in H. contortus. In AS-PCR, one reverse primer (TGG 312) was used in two separate reactions with each of 2 forward primers (resistant TGG 331 and susceptible CAW 106 primer) that differed only at 3' nucleotide position. Therefore, the amplified products from resistant and susceptible parasites were produced 267 and 266 bp, respectively. A total of 162 parasites were genotyped, of which 130 parasites found homozygous resistant 'rr', 22 heterozygous 'rS' and 10 homozygous susceptible 'SS' type. The prevalence of 'rr' individuals was higher in Jaipur (98%) followed by Avikanagar (93%) and Bikaner (50%) regions. Overall, the prevalence of BZ resistant allele (r) was higher (87%) as compared to 13% of BZ susceptible allele (S).

Seasonal Variation in miR-328-3p and let-7d-3p Are Associated With Seasonal Allergies and Asthma Symptoms in Children

Tiwari Anshul,Wang Alberta L.,Li Jiang,Lutz Sharon M.,Kho Alvin T,Weiss Scott T,Tantisira Kelan G,McGeachie Michael J. 대한천식알레르기학회 2021 Allergy, Asthma & Immunology Research Vol.13 No.4

Objective MicroRNAs (miRs) are small non-coding RNA molecules of around 18–22 nucleotides that are key regulators of many biologic processes, particularly inflammation. The purpose of this study was to determine the association of circulating miRs from asthmatic children with seasonal variation in allergic inflammation and asthma symptoms. Methods We used available small RNA sequencing on blood serum from 398 children with mild-to-moderate asthma from the Childhood Asthma Management Program. We used seasonal asthma symptom data at the study baseline and allergen affection status from baseline skin prick tests as primary outcomes. We identified differentially expressed (DE) miRs between pairs of seasons using DESeq2. Regression analysis was used to identify associations between allergy status to specific seasonal allergens and DE miRs in 4 seasons and between seasonal asthma symptom data and DE miRs. We performed pathway enrichment analysis for target genes of the DE miRs using DAVID. Results After quality control, 398 samples underwent differential analysis between the 4 seasons. We found 52 unique miRs from a total of 81 DE miRs across seasons. Further investigation of the association between these miRs and sensitization to seasonal allergens using skin prick tests revealed that 26 unique miRs from a total of 38 miRs were significantly associated with a same-season allergen. Comparison between seasonal asthma symptom data revealed that 2 of these 26 miRs also had significant associations with asthma symptoms in the same seasons: miR-328-3p (P < 0.03) and let-7d-3p (P < 0.05). Enrichment analysis showed that the most enriched pathway clusters were Rap1, Ras, and MAPK signaling pathways. Conclusion Our results show seasonal variation in miR-328-3p and let-7d-3p are significantly associated with seasonal asthma symptoms and seasonal allergies. These indicate a potentially protective role for let-7d-3p and a deleterious role for miR-328-3p in asthmatics sensitized to mulberry. Further work will determine whether these miRs are drivers or results of the allergic response.

ECR plasma etching of GaAs in CCl2F2/Ar/O2 discharge and IR studies of the etched surface

L. S. S. Singh,K. P. Tiwary,R. K. Purohit,Z. H. Zaidi,M. Husain 한국물리학회 2005 Current Applied Physics Vol.5 No.4

The etching of GaAs materials under electron cyclotron resonance conditions has been performed in an ECR etching system withrf biasing using the CCl2F2/Ar/O2 plasma chemistry. Etching experiments were carried out at a pressure between 0.015 and 0.020mbar, rf power 0.39 W/cm2, and dc bias voltage 200 V. The surface morphology and etch depth were taken by scanning electronmicroscopy and Dektek Prolometry respectively. The use of ECR conditions with additional rf biasing provides the good etchingof the surface and fast etch rates. Moreover, the surface of the GaAs material display smooth and stoichiometric surfaces at higherECR powers. The surface damages on the GaAs samples after the plasma exposure have been studied using IR spectroscopy..

PREDICTION OF BREAK-OUT NOISE OF A RECTANGULAR HVAC DUCT WITH ACOUSTIC LAGGING

B. Venkatesham,M. L. Munjal,Mayank Tiwari 한국소음진동공학회 2008 한국소음진동공학회 국제학술대회논문집 Vol.2008 No.7

Magnetic field controlled graphene oxide-based origami with enhanced surface area and mechanical properties

Park, Ok-Kyung,Tiwary, Chandra Sekhar,Yang,Bhowmick, Sanjit,Vinod, Soumya,Zhang, Qingbo,Colvin, Vicki L.,Asif, S. A. Syed,Vajtai, Robert,Penev, Evgeni S.,Yakobson, Boris I.,Ajayan, Pulickel M. The Royal Society of Chemistry 2017 Nanoscale Vol.9 No.21

Effect of Ni Doping on Magnetic Properties of LaFe<SUB>1-x</SUB>NixO₃ (x =0.4 and 0.5)

Alpa Dashora,Shailja Tiwari,H. S. Mund,R. J. Choudhary,D. M. Phase,M. Itou,Y. Sakuari,B. L. Ahuja 한국자기학회 2010 한국자기학회 학술연구발표회 논문개요집 Vol.2010 No.12

Vertical flows and mass flux balance of sunspot umbral dots

Riethmü,ller, T. L.,Solanki, S. K.,van Noort, M.,Tiwari, S. K. EDP Sciences 2013 Astronomy and astrophysics Vol.554 No.-

Cascade random-quasi-phase-matched harmonic generation in polycrystalline ZnSe

Kupfer, R.,Quevedo, H. J.,Smith, H. L.,Lisi, L. A.,Tiwari, G.,Richmond, C. G.,Bowers, B. B.,Fang, L.,Hegelich, B. M. American Institute of Physics 2018 JOURNAL OF APPLIED PHYSICS - Vol.124 No.24

Sustainable employment of folkloric botanicals and conservation practices adopted by the inhabitants of Parbati Valley of North Western Himalaya, India in healing substantial corporeal disorders

Tanay Barman,Sher Singh Samant,Jyoti,Abhijit Dey,Samapika Nandy,Riya Maitra,L. M. Tiwari,Anjana 경희대학교 융합한의과학연구소 2023 Oriental Pharmacy and Experimental Medicine Vol.23 No.2

Variations in topogeographical features causes immense miscellany of widely used medicinal plants (MPs) in Indian Himalayan Region (IHR), thus an ethnobotanical approach was adopted to file the undocumented indigenous wealth of the communities against different corporeal ailments. 370 households of 31 villages were surveyed during the summer seasons of 2015 and 2016 to explore the traditional folkloric practices of the inhabitants using a semi structured questionnaire. The data set was documented in Microsoft Office Excel and statistically analyzed for Use Value (UV), Informants' Consensus Factor (ICF), Fidelity Level (FL), Relative Importance (RI), Cultural Importance Index (CI), Index of Agreement on Remedies (IAR) and Cultural Agreement Index (CAI). The MPs were classified and analyzed for altitudinal distribution, nativity, endemism and threat categories. Total 292 MPs belonging to 95 families were reported to be used against major 15 types of substantial corporeal disorders. The richness of MPs decreased with an increasing altitude. Asteraceae (27 species) was found to be dominant family, followed by Rosaceae (17 species), Lamiaceae (16 species) and Ranunculaceae (15 species) used to cure such ailments. Five MPs were endemic to the IHR and 8 MPs were found to be critically endangered (CR) in the study region. Traditional healers had a detailed and wide range of folkloric therapeutic wisdom to treat different types of human diseases. Folkloric practice provides an alternative preference from conventional healthcare centers for the needy rural ethnic people of this distant area. But unfortunately rapid loss in biodiversity elements, urbanization and considerable anthropogenic stress causes significant decrease in the ethnic knowledge as well as the MPs. Thus, awareness among the people and proper conservation measures is readily needed.