BIM 적용사례 활용시스템 구축을 위한 기존 BIM프로젝트 사이트 분석

이경하(Kyungha Lee),이강(Ghang Lee),이화연(Hwayeon Lee) (사)한국CDE학회 2015 한국 CAD/CAM 학회 학술발표회 논문집 Vol.2015 No.하계

Prospective, Randomized Safety Assessment of Long- Term Therapy With Entecavir or Other Nucleos(t)ide Analogues in Korean Patients with Chronic HBV Infection

( Changhyeong Lee ),( Joung-il Lee ),( Kwang-hyub Han ),( Sang- Young Han ),( Jeong Heo ),( Kwan Sik Lee ),( Dong Joon Kim ),( June Sung Lee ),( Kyungha Yu ),( Alexandra Thiry ),( Elizabeth Cooney ) 대한간학회 2018 춘·추계 학술대회 (KASL) Vol.2018 No.1

Aims: REALM was a prospective, randomized, open-label, observational study, conducted to assess rates of long-term clinical outcomes in patients with CHB who were treated with entecavir (ETV) or other standard-of-care HBV nucleos(t)ide analogues (nuc). Korean sub-analysis was conducted to investigate any significant differences on clinical outcomes and safety profiles in Korean subjects. Methods: Treatment-naive or -experienced Korean patients with CHB were randomly assigned (1:1) to monotherapy with ETV (0.5 mg or 1.0 mg once daily per product label) or a non- ETV nuc (specific agent investigator-selected) and followed for ≤10 years. Clinical outcome events (COEs) were reported by investigators, reviewed and adjudicated by an Events Adjudication Committee (EAC). Primary endpoints were rates of adjudicated COEs including 1) malignant neoplasms (composite of non-hepatocellular carcinoma [HCC] and HCC); 2) liver-related HBV disease progression (composite of non-HCC HBV disease progression, HCC, and liver-related death); and 3) all-cause death. Secondary endpoints were adjudicated COE rates of non-HCC malignant neoplasms, HCC, and liver-related death. An exploratory endpoint was the adjudicated COE rate of non- HCC HBV disease progression. Treatment-related serious adverse events (SAEs) were also assessed. Results: Patients were randomized and treated to receive ETV (n=1243) or non-ETV (n=1239). Overall, at baseline, 63% were HBeAg+ and 37% had cirrhosis.. Mean time on study was 91 (ETV) and 86 (non-ETV) months. There were no statistically significant differences in COE rates between treatment groups (Table). Results were consistent in baseline subgroups. Treatment-related SAEs that were not protocol-defined COEs were reported in 2 (0.2%) ETV and 26 (2.1%) non-ETV patients, with spectra as expected. Conclusions: This large, long-term study found no statistically significant differences in COE rates between Korean patients randomized to ETV vs non-ETV nuc monotherapy, and the results of Korean sub-analysis were consistent with the results from entire REALM study subjects. SAEs were uncommon al-though higher in the non-ETV group.

Synthetic Application of Octalone Systems (Ⅰ): Synthesis of $\beta$-Cyperone

Kyunghae Lee,Bongser Park,Hongbum Kim Korean Chemical Society 1993 Bulletin of the Korean Chemical Society Vol.14 No.4

A Small Epitope Tagging on the C-Terminus of a Target Protein Requires Extra Amino Acids to Enhance the Immune Responses of the Corresponding Antibody

Lee Kyungha,Cho Man-Ho,Kim Mi-Ju,Bhoo Seong-Hee 한국미생물·생명공학회 2024 Journal of microbiology and biotechnology Vol.34 No.6

Protein-specific antibodies are essential for various aspects of protein research, including detection, purification, and characterization. When specific antibodies are unavailable, protein tagging is a useful alternative. Small epitope tags, typically less than 10 amino acids, are widely used in protein research due to the simple modification through PCR and reduced impact on the target protein's function compared to larger tags. The 2B8 epitope tag (RDPLPFFPP), reported by us in a previous study, has high specificity and sensitivity to the corresponding antibody. However, when attached to the C-terminus of the target protein in immunoprecipitation experiments, we observed a decrease in detection signal with reduced immunity and low protein recovery. This phenomenon was not unique to 2B8 and was also observed with the commercially available Myc tag. Our study revealed that Cterminal tagging of small epitope tags requires the addition of more than one extra amino acid to enhance (restore) antibody immunities. Moreover, among the amino acids we tested, serine was the best for the 2B8 tag. Our findings demonstrated that the interaction between a small epitope and a corresponding paratope of an antibody requires an extra amino acid at the C-terminus of the epitope. This result is important for researchers planning studies on target proteins using small epitope tags.

Functional identification of three regiospecific flavonoid O-methyltransferases in Rhododendron delavayi and their applications in the biotechnological production of methoxyflavonoids

Kyungha Lee,Seong Hee Bhoo,Sang‑Won Lee,Man‑Ho Cho 한국응용생명화학회 2024 Applied Biological Chemistry (Appl Biol Chem) Vol.67 No.-

Rhododendrons produce a variety of methoxyflavonoids, including rarely found 3-methoxyflavonoids and 5-methoxyflavonoids. It was thus suggested that they have a series of regiospecific flavonoid O-methyltransferases (FOMTs). The 18 Class II O-methyltransferase (OMT) genes were retrieved from the Rhododendron delavayi genome, designating them as RdOMTs. A comprehensive biochemical characterization of RdOMTs was performed to identify functional FOMTs. The FOMT activity of recombinant RdOMTs was assayed with flavonoid substrates of different subclasses. Among the examined RdOMTs, RdOMT3, RdOMT10, and RdOMT12 showed FOMT activity for diverse flavonoids. In particular, RdOMT3 consumed only flavonols as a substrate. Structural analyses of the methylated products demonstrated that RdOMT3, RdOMT10, and RdOMT12 catalyze regiospecific methylation of flavonoids at the 3’/5’-, 3-, and 4’-hydroxyl groups, respectively. Their broad substrate spectrum and different regiospecificity suggest that these RdOMTs contribute to the formation of complex methoxyflavonoids in R. delavayi. Bioconversion of flavonoids using E. coli harboring each RdOMT demonstrated that RdOMT3, RdOMT10, and RdOMT12 are useful tools for the biotechnological production of valuable methoxyflavonoids, including the rarely found 3-methoxyflavonoids. Rhododendrons produce a variety of methoxyflavonoids, including rarely found 3-methoxyflavonoids and 5-methoxyflavonoids. It was thus suggested that they have a series of regiospecific flavonoid O -methyltransferases (FOMTs). The 18 Class II O -methyltransferase (OMT) genes were retrieved from the Rhododendron delavayi genome, designating them as RdOMTs. A comprehensive biochemical characterization of RdOMTs was performed to identify functional FOMTs. The FOMT activity of recombinant RdOMTs was assayed with flavonoid substrates of different subclasses. Among the examined RdOMTs, RdOMT3, RdOMT10, and RdOMT12 showed FOMT activity for diverse flavonoids. In particular, RdOMT3 consumed only flavonols as a substrate. Structural analyses of the methylated products demonstrated that RdOMT3, RdOMT10, and RdOMT12 catalyze regiospecific methylation of flavonoids at the 3'/5'-, 3-, and 4'-hydroxyl groups, respectively. Their broad substrate spectrum and different regiospecificity suggest that these RdOMTs contribute to the formation of complex methoxyflavonoids in R. delavayi . Bioconversion of flavonoids using E. coli harboring each RdOMT demonstrated that RdOMT3, RdOMT10, and RdOMT12 are useful tools for the biotechnological production of valuable methoxyflavonoids, including the rarely found 3-methoxyflavonoids.

A FRAMEWORK FOR RELIABLITY ASSESSMENT ON THE OPERABILITY OF THERMALLY AGED AIR-OPERATED VALVE

KyungHa Ryu(류경하),TaeHyun Lee(이태현),SangHyuk Lee(이상혁),DaeHwan Kim(김대환) 대한기계학회 2015 대한기계학회 춘추학술대회 Vol.신뢰성 No.-

管의 전통, 그 일상의 정치

이경하(Lee, Kyungha) 국문학회 2007 국문학연구 Vol.15 No.-

One topic of this essay is that women s Haengjang(行狀), records of a dead person s life, and Myojimyong(墓誌銘), a kind of epitaphs, originated from a tradition of Donggwan(彤管). In ancient China, Donggwan originally meant documents that Yeosa(女史), a female historiographer, recorded daily lives of queens, and the meaning changed to a history of ideal women in Joseon Dynasty. In this essay, I regard women s Haengjang and Myojimyong as writing belonged to tradition of Donggwan as well as tradition of writing for the dead . Another topic is to discuss what value women s Haengjang and Myojimyong have as historial materials. Scarcity of materials and historical records is one of big problems in studies of women s classical literary history. In this viewpoint, Haengjang and Myojimyong are very important sources for women s history. Because so many women s Haengjang and and Myojimyong were written during Joseon Dynasty and the documents often contained many kinds of informations about women s daily lives. However, women s daily lives were usually represented as very normative forms, which spoke for gender politics in women s daily lives in the Middle Ages.

한국 BIM 도입 정책과 특징 연구

이경하(Kyungha LEE),이강(Ghang LEE) 대한건축학회 2021 대한건축학회 학술발표대회 논문집 Vol.41 No.1

1976년 톈안먼 광장의 기록 - 『톈안먼시초(천안문시초(天安門詩抄))』(1978)의 탄생과정 연구

李庚夏 ( Lee Kyungha ) 중국어문학회 2020 中國語文學誌 Vol.0 No.72

The Tian’anmen Poems was originated from the Tian’anmen Poetry Movement, which was the movement that the countless people who filled Tian’anmen Square before and after the Qingming Festival in 1976 were expressed their cherish memory on Zhou Enlai and anger of ‘Gang of Four’ on many wall posters. Thus, The Tian’anmen Poems has been read and studied with political weight and implications from the beginning. However, from the perspective of foreign researchers who can maintain an objective distance geographically and periodically, it is believed that the meaning of the study of The Tian’anmen Poems is to examine the process of its birth, and this paper focused on the following contents. Firstly, the previous studies published in China were introduced by period and their characteristics were briefly analyzed. The main research achievements related to the Tian’anmen Poetry Movement since 2000 have been able to broaden the horizon of research by boldly raising issues with skepticism, freeing from the attitude of blind admiration and praise. It can be pointed out that it has become possible to promote the quality of growth of the related researches. Secondly, we investigated the generation and development process of the Tian’anmen Poetry Movement, and through this, we can see how much hardship has suffered by numerous authors, mortals, and collectors who participated in the Tian’anmen Poetry Movement. Thirdly, the process of The Tian’anmen Revolutionary Poems, which was printed in duplicate in 1977, was published in the unofficial publication The Revolutionary Poems, the first printed version of the ‘Tong Huaizhou’ editorial team version and ‘The seventh Ministry of machinery industry’ editorial team version. Fourthly, by dividing the appearance of The Tian’anmen Poems as an official publication into “changed status according to the changes of the times” and “characteristic of the preface as a legitimate publication”, We had an opportunity to recognize the special political landscape of China once again.

BIM프로젝트의 수집 및 활용을 위한 구조화된 사례분류체계 개발

이경하(Lee, Kyungha),이강(Lee, Ghang) 대한건축학회 2017 대한건축학회 학술발표대회 논문집 Vol.37 No.2

The purpose of this study was to develop a BIM (Building Information Modeling) project information–classification system to collect and analyze BIM project data. To develop the classification system, previous studies on construction project classification systems were reviewed first. In parallel, the project factors that were studied in 128 BIM case studies were analyzed. An early version of a BIM project information classification was quantitatively and qualitatively reviewed in five categories by a total of five practitioners with BIM experience. The five categories were importance, appropriateness, urgency, specificity, and collectability. The classification has been modified based on the review results.