Histone deacetylase 3 is selectively involved in L3MBTL2-mediated transcriptional repression

Yoo, Jung-Yoon,Choi, Kyung-Chul,Kang, HeeBum,Kim, Young Jun,Lee, Jeongmin,Jun, Woo Jin,Kim, Mi-Jeong,Lee, Yoo-Hyun,Lee, Ok-Hee,Yoon, Ho-Geun Elsevier 2010 FEBS letters Vol.584 No.11

<P><B>Abstract</B></P><P>This is the first report that L(3)mbt-like 2 (L3MBTL2) specifically interacts with the histone deacetylase domain of histone deacetylase 3 (HDAC3) via its MBT domain. Here, we show that L3MBTL2 selectively interacts with HDAC3, but not other class I HDACs. An in vitro peptide-binding assay demonstrated the specific association of HDAC3 with methylated histone-K20 tail and L3MBTL2. Furthermore, depletion of HDAC3 resulted in a decrease of methylated K20-H4, as well as an increase in acetylated histone H3. Consequently, HDAC3 knock-down selectively suppressed L3MBTL2-mediated transcriptional repression. Taken together, our results reveal the concerted action of both HDAC3 and L3MBTL2 in histone deacetylation and methylation-dependent transcriptional repression.</P><P><B>Structured summary</B></P><P>MINT-7719975: <I>L3MBTL2</I> (uniprotkb:Q969R5) and <I>HDAC3</I> (uniprotkb:O15379) <I>colocalize</I> (MI:0403) by <I>fluorescence microscopy</I> (MI:0416)</P><P>MINT-7719941, MINT-7719921: <I>L3MBTL2</I> (uniprotkb:Q969R5) <I>binds</I> (MI:0407) to <I>HDAC3</I> (uniprotkb:O15379) by <I>pull down</I> (MI:0096)</P><P>MINT-7719991: <I>HDAC3</I> (uniprotkb:O15379) <I>physically interacts</I> (MI:0915) with <I>L3MBTL2</I> (uniprotkb:Q969R5) by <I>anti bait coimmunoprecipitation</I> (MI:0006)</P><P>MINT-7719958: <I>L3MBTL2</I> (uniprotkb:Q969R5) <I>physically interacts</I> (MI:0915) with <I>HDAC3</I> (uniprotkb:O15379) by <I>anti tag coimmunoprecipitation</I> (MI:0007)</P><P>MINT-7719897: <I>HDAC3</I> (uniprotkb:O15379) <I>physically interacts</I> (MI:0915) with <I>L3MBTL2</I> (uniprotkb:Q969R5) by <I>two hybrid</I> (MI:0018)</P>

八味元이 Streptozotocin 糖尿病에 미치는 影響

김희경,문정숙,박귀영,이다미,이은영,장윤경,황미경 曉星女子大學校 藥學大學 學生會 1988 曉星藥誌 Vol.4 No.-

Streptozotocin was injected to rats and rabbits to induce the experimental diabetes mellitus and Palmiwon was administrated to observe the changes of blood sugar, body weight, and serum Zn, Ca, Mg level in order to find out the effect of diabetic tratment by experimental method. And thus, the result was as follows: 1. The blood sugar level of diabetic rat was decreased after administration of Palmiwon. 2. Body weight of diabetic rat was moderately decreased after administratin of Palmiwon. 3. The less streptozotocin, 30 mg/kg, don't easily induce diabetes mellitus in rabbits. We did not find the changes of blood sugar, serum Zn, Ca, Mg level in the rabbits injected by streptozotocin, 30mg/kg of body weight.

창업보육센터 지원서비스가 창업기업 만족도에 미치는 영향: 서울지역 BI 운영주체의 조절효과를 중심으로

이미경(Lee, Mi-Kyung),김정인(Kim, Jeong-In) 한국경영교육학회 2020 경영교육연구 Vol.35 No.3

[연구목적] 본 연구는 공공과 민간으로 구분한 창업보육센터 운영주체의 지원서비스가 보육센터 입주기업들의 만족도에 어떠한 영향을 미치는지를 분석하고, 유의한 영향을 미치는 서비스를 규명하여 지원서비스 체계의 개선과 입주기업의 만족도를 얻기 위한 효과적 방향을 제시하고자 하였다. 민간과 공공의 창업보육 기여도에 대한 연구의 필요성을 제언한 선행연구에도 실증적인 연구가 미진하다. 이에, 공공과 민간 창업보육센터의 지원서비스와 만족도 간에 공공과 민간 운영주체의 조절효과를 실증하고, 창업보육정책을 위한 시사점을 제공하고자 하였다. [연구방법] 창업보육센터, 운영주체, 창업보육지원서비스, 만족도 등 관련 이론과 선행연구를 바탕으로 연구의 필요성을 확인하였다. 이를 위해, 창업보육센터 지원서비스 중 만족도에 유의한 영향을 미치는 기본가설과 조절효과 등 8개의 가설을 도출하였고, 공공과 민간센터 입주고객을 대상으로 자료를 수집하였다. 수집된 자료에 대해 신뢰성과 타당성을 검토하였으며 SmartPLS 2.0의 경로분석을 통해 가설을 검증하였다. [연구결과] 창업보육센터를 통한 지원서비스 대한 필요성을 재확인했다. 또한, 민간 영리섹터의 행정 및 네트워크 서비스가, 비영리 공공센터의 물리적 서비스와 전문 지원서비스가 입주기업의 만족도 간에 조절효과가 있는 것으로 나타났다. [연구의 시사점] 기업의 성과와 만족도에 영향을 미치는 창업지원 서비스 영역에서도 공공과 민간이 제공하는 서비스 품질에 대한 만족도를 제고하여, 공공과 민간 운영주체의 지원서비스에 대한 이론적, 실무적 시사점 및 창업보육 정책의 연구에 기여하고자 하였다. [Purpose] This study analyzes how support services of business incubators, divided into public and private sectors, affect satisfaction of resident companies. The purpose of this study was to demonstrate the moderating effect of public and private operating entities between support services and satisfaction within public and private incubating centers, and to provide implications for incubating center policies. [Methodology] This study derived eight hypotheses, from the basic hypothesis and to those of the moderating effect, with significance in satisfaction with incubating centers, operating entities, incubating center support services, and overall satisfaction. Data was collected from 151 companies in public and private incubating centers. Reliability and validity of the collected data were reviewed, and the hypothesis was verified through route analysis of SmartPLS 2.0. [Findings] The results of the study reaffirmed the need for support services in incubation centers, but administrative and network services of private for-profit sector centers, and physical and professional support services by public centers of the non-profit sectors, adjusted the satisfaction result between resident companies. [Implications] In this study, the field of enterprise support service, which affects the performance of the company, satisfaction levels evidence the quality of service provided by public and private sectors and can provide theoretical and practical support for business incubator operators from both public and private sectors. It was intended to contribute to the study of implications and business incubation policies.

의약분업 전후의 의약정보서비스의 질의응답과 질의자의 만족도 분석

신정인,김미애,허경희,김미정,신현택,오정미 한국병원약사회 2003 병원약사회지 Vol.20 No.2

Drug Information Centers(DICs) are responsible for providing updated and relevant drug information on the efficacy, safety and quality of drugs to health-care practitioners and finally to patients. After the establishment of new prescription law(Bunup)' on August 2000, the future direction for DICs is strongly needed to provide specified, appropriate and rapid information to health-card practitioners and patients. This project was undertaken to provide a future direction of DICs via comparing differences of Q&A before and after Bunup, based on the analysis of Q&A worksheets that were conducted from August 1999 to July 2001 at Drug Information Research Institute(DIRI) of Sookmyung Women's University. In addition, feedback sheets were collected from the users of DIRI from April 2001 to August 2001, to evaluate the satisfaction scores by '5-point Likert scale' on the response. The number of inquiries responded by DIRI was increase from 201 to 574 and the most frequently asked inquirers were pharmacists, specifically community pharmacists. The mean time to respond before and after Bunup was 5.35 and 4.68 hours, respectively. The method of inquiry utilized the most was electronic mailing system followed by telephone both before and after Bunup(66.2% vs. 65.7% via e-mail, from 32.8% vs. 32.4% via telephone). The most frequently asked category of question was on the 'clinical drug' category both before and after Bunup (79% vs. 73.6%). The tertiary literature was the most frequently used reference to answer the inquiries. Mean feedback result via questionnaires was good. That included accuracy, quality of information, time to respond, attitude of provider, accessibility, etc. The most increased inquirer after Bunup was general person, compared with before. Therefore DICs should provide separate responds according to inquirers, develop the systemic program to improve the response for each request, have systemic Q&A worksheet, and develop the educational program for drug information providers. DICs should continuously provide more appropriate and rapid information to health-care practitioners and patients.

심근 허혈-재관류 백서 모델에서 mitogen-activated protein kinase 활성화에 대한 α-lipoic acid의 효과

김연경,박은미,최준호,윤경호,유남진,이은미,김남호,정진원,김민선,박병림,오석규 圓光大學校 醫科學硏究所 2006 圓光醫科學 Vol.21 No.1

남성화를 보이는 여성에서 발견된 난소의 Steroid Cell Tumor 1예

조인호,정대훈,박영미,서영진,손영실,정철회,강영미,정수전,김영남,이경복,성문수,김기태 인제대학교 2006 仁濟醫學 Vol.27 No.-

Steroid cell tumor is a rare ovarian sex cord-stromal tumor which accounts for 0.1% of all ovarian tumors. Until now, only 4 cases have been reported in domestic literatures. Steroid cell tumor often secrets testosterone and presents virilization in adult women or precocious puberty in children. Treatment is often performed by surgical removal, adjuvant chemotherapy and radiation, but completely accepted treatment was not existed. We experienced a case of steroid cell tumor, which was manifested by typical virilization in a 43-year old patient, who was previously performed hysterectomy and unilateral oophorectomy. So, we present with a brief review of the literatures.

대동맥 협착증과 동반된 출혈성 위장관 동정맥 기형에서 Estrogen-Progesterone 치료 1예

김도연,김화정,김효정,남준식,박수영,박영미,신정은,주미순,황민규,정혜경,손혜영,이선영,이한주 大韓消化器內視鏡學會 1999 Clinical Endoscopy Vol.19 No.부록2

한국인 직무 스트레스 측정도구의 개발 및 표준화

장세진,고상백,강동묵,김성아,강명근,이철갑,정진주,조정진,손미아,채창호,김정원,김정일,김형수,노상철,박재범,우종민,김수영,김정연,하미나,박정선,이경용,김형렬,공정옥,김인아,김정수,박준호,현숙정,손동국 大韓産業醫學會 2005 대한직업환경의학회지 Vol.17 No.4

Background and Purposes: Over the past three decades, numerous studies performed in Korea have reported that job stress is a determinant risk factor for chronic diseases and work disability. Every society has its own culture and occupational climate particular to their organizations, and hence experiences different occupational stress. An occupational stress measurement tool therefore needs to be developed to estimate it objectively. The purpose of this study is to develop and standardize the Korean Occupational Stress Scale (KOSS) which is considered to be unique and specific occupational stressors in Korean employees. Subjects and Methods: Data were obtained from the National Study for Development and Standardization of Occupational Stress (NSDSOS Project: 2002-2004). A total of 12,631 employees from a nationwide sample proportional to the Korean Standard Industrial Classification and the Korean Standard Occupational Classification were administered. The KOSS was developed for 2 years (2002-2004). In the first year, we collected 255 items from the most popular job stress measurement tools such as JCQ, ERI, NIOSH and OSI, and 44 items derived from the a qualitative study (depth interview). Forty-three items of KOSS, in the second year, were retained for use in the final version of the KOSS by using Delphi and factor analysis. Items were scored using conventional 1-2-3-4 Likert scores for the response categories. Results: We developed eight subscales by using factor analysis and validation process: physical environment (3 items), job demand (8 items), insufficient job control (5 items), interpersonal conflict (4 items), job insecurity (6 items), organizational system (7 items), lack of reward (6 items), and occupational climate (4 items). Together they explained 50.0% of total variance. Internal consistency alpha scores were ranged from 0.51 to 0.82. Twenty-four items of the short form of the KOSS (KOSS-SF) were also developed to estimate job stress in the work setting. Because the levels of the subscales of occupational stress were gender dependent, gender-specific standard norms for both the 43-item full version and the 24-item short form using a quartile for the subscales of KOSS were presented. Conclusion: The results of this study suggest that KOSS might be an appropriate measurement scale to estimate occupational stress of Korean employees. Further and more detailed study needs to be conducted to improve the validity of this scale.

비만을 동반한 위암 환자에서 두 병을 같이 수술 치료한 경험(증례)

최경현,윤기영,문형환,신연명,서경원,안수미,송윤미,석정희,정경연,이은하 고신대학교의과대학 2008 고신대학교 의과대학 학술지 Vol.23 No.3

Obesity is growing problem in Korea. We had a case of bariatric surgery during gastric cancer operation. Patient was 29 year old Korean lady with early gastric cancer located in the lesser curvature side of the middle 1/3 of the stomach. Her body weight was 89 kg, height 163 cm, and thus body mass index was 33.5 kg/m2. Preoperative blood pressure was 130/90 mmHg, hemoglobin 12.9 g/dL, total lymphocyte count 3,280/mL, serum albumin 4.3 g/dL, CEA 1.1 ng/mL, CA 19-9 9.1 ng/mL, and alpha fetoprotein 2 ng/mL. Another associated disease was right thyroid follicular neoplasm. The chest X-ray was normal. After IM injection of 2,500 units of heparin 30 minutes before the induction of general anesthesia, she received curative subtotal gastrectomy and Roux en Y gastrojejunostomy when a malabsorption loop of 120 cm jejunum was incorporated between the Treitz ligament and the end to side jejuno-jejunostmy site at May 29th 2006. Her postoperative course was uneventful except a minor wound seroma and the elevations of serum amylase(up to 4 folds) and lipase(up to 2 folds). She lost her body weight 9 kg in 5th, 21 kg in 7th, and 27 kg in 10th postoperative months to became 61.5 kg. On follow up exams in July 2008, she gained 4 kg to overcome her weakness and fatigue. From the above result, the incorporation of a malabsorption loop during reconstruction phase of gastric cancer operation was a good option for obese gastric cancer patients especially in cases of early gastric cancer.

개에서 발생한 양파 섭취에 의한 하인즈 소체 용혈성 빈혈 증례

이미선,윤기영,서경원,최호정,정성목,송근호,조종기,박성준 忠南大學校 獸醫科大學 附設 動物醫科學硏究所 2012 動物醫科學硏究誌 Vol.19 No.1

A 10 month-old Yorkshire Terrier was referred to the Veterinary Medical Teaching Hospital of Chungnam National University with the history of lethargy, bile pigment in urine and anorexia. Blood smear showed Heinz body, polychromatocytosis, schistocytosis, anisocytosis, which were the results from the oxidative injury. Hematology demonstrated a moderate anemia(PCV 22.4%, reference rage 35-55%), which was regeneration based on the degree of RPI (4.03, reference rage > 1). Serum biochemistry demonstrated mild elevation in total bilrubin, and mild decrease in total protein and albumin concentration. A diagnosis of Heinz body hemolytic anemia secondary to onion ingestion was made and treated with fluid therapy, oxygen and antioxidant drugs. The dog recovered without any complication.