肝吸蟲感染에 따른 血淸 IgE와 血液內好酸球의 變動樣相에 관한 硏究

朱炅煥,林漢鍾 고려대학교 의과대학 1982 고려대 의대 잡지 Vol.19 No.1

The present study aimed to clarify the influence of single or repeated infection of Clonorchis sinensis on the IgE level and eosinophil counts of rabbit, and to determine the relationship between degree of infection and eosinophil counts or IgE level in the patients infected with C. sinensis. Various numbers of metacercariae isolated from flesh of the second intermediate host, Pseudorasbora parva, were directly introduced to the rabbits. Single infection group was divided into heavy, moderate and light infection groups, in which 2,000, 500 and 50 metacercariae of C. sinensis were administered respectively. Repeated infection group was also divided into heavy, moderate and light infection groups, in these cases 200, 100 and 10 metacercariae were fed biweekly for five times respectively. Infection was confirmed by detection of the eggs of C. sinensis in feces five weeks after primary infection. Whole blood and sera were collected prior to infection, and on 1,3,5,7, and 9 weeks after infection from the inner marginal vein of rabbit's ear respectively. As a control, whole blood and sera were also sampled from the non-infected rabbits. Total eosinophil counts, differential count, and IgE levels were examined. On the other hand, a total of 50 patients infected with C. sinensis was examined their degree of infection (EPG), total eosinophil counts and differential counts of white blood cells of 50 c. sinensis infected patients were exanined. Forty-six sera were sampled from patients infected with C. sinensis and the serum IgE levels were evaluated. In analysis of serum IgE level, Phadebas IgE PRIST® radioimmunoassay technique was applied. The results are as follows: 1. Total eosinophil counts of repeated infection group of rabbits were significantly higher than that of single infection group. Total eosinophil counts of non-infected rabbits showed ranges of 0~65/㎣ blood during 9 weeks of examination. In the infected rabbits of heavy infection group, total eosinophil counts increased rapidly and reaching to peak on the 5th week after infection. These values maintained until the end of examination in 9th week (140/㎣ in single and 250/㎣ in repeated group). However, total eosinophil counts of light and modertate infection did not show rapid increase. On the contrary they increased slightly and continuously until 9 weeks after infection. Similar results were obtained from the differential counts of eosinophil. 2. In the examination of the patients infected with C. sinensis correlation between EPG and eosinophil count was recongnized. Coefficient of correlation was, r=0.61, rank correlation r=0.700, and regression equation was Yc(total eosinophil counts)=0.04χ (EPG)+372. 3. Total serum IgE levels in the patients infected with C. sinensis showed remarkably elevated value. mean values were 1,167 IU/㎖±1,172, but correlation with degree of infection was not significant statistically. Influences of repeated infection of C. sinensis on the IgE level were not able to investigate, because IgE was not detected in Phadebas IgE PRIST® using rabbit as an experimental animal.

有鉤囊尾蟲症 診斷에 있어서 特異 抗原帶의 증명에 관한 硏究

朱炅煥,姜星鎬,李駿商,林漢鍾 고려대학교 의과대학 1987 고려대 의대 잡지 Vol.24 No.3

Human cysticercosis caused by the larval stages of cestode Taenia solium is important public health problem in many of the developing countries including Korea. The application of computerized axial tomography has greatly assisted the diagnosis of neurocysticercosis. However many cases require serological examination for diagnostic confirmation. But there are some limiting factors in seroimmunological diagnosis for the detection of antibodies in serum or cerebrospinal fluid. One of the major problems is the non-specificity of the antigens which produce cross reaction with antibodies produced by the presence of other helminthic infections. Especially the sensitive tests such as ELISA are highly dependant on the purity of antigens used. To solve this problem many workers have tried to find species specific components of the antigens. Accordingly, the present studies were performed to determine the antigenic profile of the vesicular fluid and parenchymal antigens of metacestodes of Taenia solium based on SDS-PAGE and immunoblotting techniques. Analysis of human serum reactivities to these antigens gave the following results. 1. The SDS-PAGE of CyF₁ (vesicular fluid antigen) showed protein bands at the 116-200K, 106K, 91K, 63K, 21K and 2 faint bands at 97K, and 80K molecular weight region. Among them first 2 masses of bands were non-specific and the EITB patterns showed species specific reactive bands at the 91K, 63K, and 21K region. 2. The SDS-PAGE of CyL₁ (larval antigen) showed protein bands at the 116-200K, 106K, 91K, 80K, 63K, 43K, 31K, 21K, 14K and many faint bands. Among them, 3 bands at the region of 63K, 43K, and 14K showed species specific reactions. 3. The antibody produced by P. westermani, Sparganum and D. latum infected patients showed non-specific cross reaction with 116-200K, and 106K bands of CyF₁ and all bands of 80K, 116K and 116-200K bands of CyL₁. 4. The band at the region of 63K of both antigen showed strong positive reaction without interference and cross reaction of other helminthic infectons. Serum of nine Cysticercus infected patients examined showed positive reaction at the band of 63K without exception. The high species-specificity demonstrated by the present methods could be used to confirm serologically an infection by Taenia solium metacestodes.

STI571 (Glivec^(�))에 의하여 빠른 치료 반응을 보인 불응성 Gastrointestinal stromal tumor

윤경원,이제중,김여경,황호인,이병환,조상희,정익주,김형준 全南大醫大 2001 전남의대학술지 Vol.37 No.4

땅비싸리와 좀땅비싸리의 형태적변이 및 분류

조숙경,최병희,김주환 대전대학교 이과대학 기초과학연구소 1997 自然科學 Vol.- No.-

동북아시아에 분포하는 땅비싸리와 좀땅비싸리의 분류학적 한계를 규명하기 위하여 형태적 변이와 염색체수가 조사되었다. 땅비싸리의 이명으로 취급되어 왔던 좀땅비싸리는 소엽의 모용, 꽃의 크기, 화분형태, 지리적 분포 등에 있어 땅비싸리와 뚜렷한 차이를 보였으며, 또한 염색체수가 4배체(2n=32)로 나타나, 2배체(2n=16)인 땅비싸리와 차이를 보였다. 땅비싸리와 좀땅비싸리 11 집단을 대상으로 19개의 외부형태 형질에 대한 주성분분석을 실시한 결과 두 종은 제1주성분에 의해 뚜렷이 나누어 졌는데, 전체분산에 대한 제1주성분의 기여율은 63.2%이었으며, 제1주성분은 소엽 표면의 털과 화서의 길이 등과 높은 상관성을 보였다. 이러한 결과로부터 좀땅비싸리는 땅비싸리와는 구별되어 독립된 종으로 처리되었다. 한편 큰땅비싸리는 땅비싸리의 변이의 한 형태로 판단되므로 땅비싸리의 이명으로 처리되었다. 각 종의 지리적 분포에 대한 분류학적 의의가 염색체수 변이 등과 비교논의 되었다. The morphological analysis and cytological study of Indigofera kirilowii Maxim. and Indigofera korean Ohwi were undertaken to assess the numbers and ranks of taxa that usefully could be recognized. I, koreana, previously recognized as I. kirilowiii, was separated from I. kirilowii in hairiness on leaflets, flower size, pollen exine sculptures, geographical distribution and chromasome number. Chromosome count of 2n=32 (tetraploidy) from I. koreana was obtained for the first time. The results of principal components analysis (PCA) on a matrix of 11 populations of Korea by 19 morphological characters indicated that two species could be recognized by the first principal component, contribution of which for the total variance is 63.2%. Our results supported that I. koreana could be clearly distinguished from I. kirilowii. Since the quantitative differences between the two varieties, I. kirilowii var. coreana and var. Kirilowii, were smaller, a nomenclatural synonymy was developed for them, ie, var. kirilowii.

체외 충격파 쇄석기에 의해 발생된 음향 흐름 현상 연구

최민주,이승환,홍경진,김호찬,박재우,이헌주 제주대학교 공과대학 첨단기술연구소 2001 尖端技術硏究所論文集 Vol.12 No.2

Acoustic streaming produced by an extracorporeal shock wave lithotripter was visualized for the first time in the present study. It was observed that the streaming velocity increased with the shock wave amplitude and was reaching up to 3 cm/s at the highest output setting of the lithotripter.

Immunoblot법을 이용한 Anisakis증의 혈청학적 진단

이영주,주경환,정명숙,임한종 고려대학교 의과대학 1990 고려대 의대 잡지 Vol.27 No.1

Acute gastric anisakiasis is rather easily diagnosed by endoscopy or radiological technique. But preoperational diagnosis of acute intestinal anisakiasis is very difficult. Furthermore, the diagnosis of chronic anisakiasis is more difficult because the worm invade the stomach or intestinal wall and finally to degenerate. Serodignosis may helpful in such diagnostic difficulties. However, any of the serodiagnostic method in anisakiasis has limitations for practical use due to cross reactions. In this context, the study was performed to observe the specifically reacting antigenic bands of crude extract Anisakis larval worm antigen and their reaction to the serum antibody obtained from experimentally immunized and infected rabbit, confirmed human anisakiasis and other helminthic infection cases using SDS-PAGE and EITB. The results obtained were as follows; 1. Among 12 cases of confirmed anisakiasis, eleven showed positive reactions by ELISA. The average 0. D. value of the test was 1.035. Two from 4 D. latum infected cases and one from 4 A. lumbricoides infected cases showed positive reaction respectively. 2. Silver stained SDS-PAGE of crude Anisakis larval worm antigen revealed more than 45 antigenic protein bands. Among them, 186, 76, 72, 65, 59~62, 54, 52, 50, 49, 46, 43, 40, 33, 32, 29, 24, 23, 18, 17, and 13Kd bands were major bands. Protein bands of 12Kd relative molecular weight was observed in A. suum extract antigen, on the other hand 29 and 46Kd bands were not observed. 3. Major antigenic bands of crude Anisakis worm antigen which reacted with serum antibody of confirmed anisakiasis cases were 186, 116, 72, 52, 46, 40, 33 and 32Kd band. Most of them also reacted with the sera of D. latum, A. lumbricoides, T. trichiura infected cases except 46Kd band which showed positive resuits only with sera of anisakiasis. So, this band could be considered as diagnostic band for anisakiasis especially for the identification of cross reactions against A. lumbricoides and D. lafum infection. 4. Although, low molecular weight bands such as 33 and 32Kd were observed as species specific, the sensitivity of that fractions were obviously low. Consequently, the diagnostic use of the low molecular weight protein bands were not promising. 5. Serum of rabbit immunized with Anisakis larval worm antigen also showed positive reaction on 46Kd bands, but that of A. suum and T. canis did not. These results were in accord with the results in human cases. In conclusion, 46Kd bands of crude Anisakis larval worm antigen can be considered as a diagnostic one for the identification of cross reactions using immunoblot technique.

肝吸蟲症 濃厚流行地(金海市)에 있어서의 疫學的 推移에 관한 硏究

朴洙培,朱炅煥,林漢鍾 고려대학교 의과대학 1984 고려대 의대 잡지 Vol.21 No.1

In order to observe the epidemiological changes of clonorchiasis in the high endemic area in Korea, the stool examinations were carried out in Bulam-dong, Kimhae City. A total of 423 stool specimens collected from the inhabitants of this area were examined both by cellophane thick smear method and Stoll’s egg dilution counting technique. The result of this survey was compared to the data obtained in 1973 by the quantitative analysis applying the regression equations, the simple and two-stage catalytic curves. The metacercarial density of Pseudorasbora parva and the tendency of familial aggregation of this area were also compared with those of 1973. In addition to this study informal interview were conducted to investigate the KAP (knowledge, attitude and practice) of the raw fish eating habit of the ingabitants in this area. The results are as follows: 1. Overall prevalence rate of clonorchiasis was reduced from 68.3% to 45.6% in a period of 10 years. 2. In 1973, both sexes in all age groups showed almost parallel high infection rates. However in 1983, the rate for males was slightly higher than that for females and a marked reduction in the prevalence was encountered in the youngest age group. 3. The mean E.P.G. count of Clonorchis sinensis infection in the surveyed area was 10,846 in 1973. But the mean E.P.G. count was decreased to 4,858 in 1983. The degree of intensity of Clonorchis sinensis infection determined by E.P.G. count was evenly distributed in geavy, very heavy, light and moderate infections in 1973 whereas an E.P.G. of over 10,000 was seen only in 14.0% of examinee in 1973. 4. The two-stage catalytic model was applied to this study and the calculation led to the equation y=1.042(e^(-0.003t)-e^(-0.074t)); a>b in 1973, and y-1.130(e^(-0.003t)-e^(-0.026t); a>b in 1983. 5. The intensity of endemicity was compared with the regression equations calculated from cumulative percentages of E.P.G. counts. The regression equations were y=2.75+1.46 log^(χ)(Cs. D_(50) 64.76) in 1973 compared to y=3.27+1.59 log^(χ)(Cs. D_(50) 12.25) in 1983. 6. In high endemic area such as Kimhae City the tendency of familial aggregation was recognized not in 1973 but in the present study. 7. Metacercarial infestation rate of P.parva did not change but metacercarial density was decreased from 1,312.4 ea/fish to 1,233.4 ea/fish in 1972 and 1973 to 160.8 ea/fish in 1983. 8. The KAP(knowledge, attitude and practice) of raw-fish eating habit of inhabitants were mentioned.

조직기생 선충류 유충에서 분리한 단백 분해 효소의 특성 및 항원성 검토

임한종,주경환,최성아,이혜정,주종윤,정명숙 한국농촌보건회 1997 농촌의학·지역보건 Vol.22 No.1

有鉤囊尾蟲症의 血淸學的 診斷에 있어서 酵素免疫 電氣泳動 blot 법의 효용성에 관한 연구

許南鎭,朱炅煥,林漢鍾 고려대학교 의과대학 1988 고려대 의대 잡지 Vol.25 No.1

The applicability of enzyme-linked immunoelectrotransfer blot (western blot) was evaluated in human cysticercosis using samples of serum. A total of 40 confirmed cysticercosis patients, 14 taeniasis,7 D. latum, 9 P. westermani, 16 C. sinensis, 1 sparganum infected patients and 20 normal controls were subjected in this study. Vesicular fluid and parenchymal antigen of C. cellulosae obtained from naturally infected pig was used as antigen in protein concentration of 2.4mg/ml and 3.7 mg/ml respectively. Gradient SDS-PAGE was performed and blotted electrophoretically onto a sheet of nitrocellulose as described by Tsang et al (1983). The sheet was cut into strips and exposed to various sera diluted 1:200 with PBS. Antigen bands of 91Kd, 63Kd, 43Kd, and 14Kd as described by Joo at al(1987) were visualized by EITB using antihuman IgG peroxidase. The results were summarized as follows: 1. The sensitity of western blot using vesicular fluid was 95.0% in 40 cysticercosis patients, which was slightly higher than 90.0% obtained by microplate ELISA. 2. The sensitivity of western blot using parenchymal antigen was 90.0% compare to 75.0% sensitivity of ELISA. 3. Twenty healthy controls did not show any false positive reaction in both techniques. With ELISA method, taeniasis (3/14), sparganosis (1/1) paragonimiasis (1/9), diphyllobothriasis (2/7) cases showed cross reactions using vesicular fluid antigen. But cross reactions did not occur in western blot except taeniasis cases. The above results indicate that western blot technique was applicable to the diagnosis of cysticercosis and helpful for the elimination of cross reactions by other helminthic infections.

腦囊尾蟲症의 化學的療法에 관한 臨床的 硏究

朴昌潤,朱炅煥,林漢鍾 고려대학교 의과대학 1982 고려대 의대 잡지 Vol.19 No.3

The purpose of this study was to evaluate the therapeutic effect of praziquantel (Embay8440, Biltricide®) on human cerebral cysticercosis. The praziquantel is a broad spectrum anthelmintic agent and has a high activity against cestodes and trematodes. Before and after the treatment with oral administration of the praziquantel the changes in structure of Cysticercus cellulosae biopsied in subcutaneous tissue were studied by scanning and transmission electron microscope, and cerebral cysticercosis by computerized tomographic scanning. The results were as follows : 1. The findings of the observations on subcutaneous Cysticercus biopsied after treatment were compared with those of before treatment. At the early stage of the treatment, small vacuoles were scattered along the basement layer in the tegumental syncytium of the scolex and neck regions. In the scanning electron microscopic observation, marked surface changes were present in the neck region with many bleb-like structures formed by the bursting of the large vacuoles in the tegumental syncytium. In the specimens biopsied at 2 or 5 weeks after treatment, the degenerations and necrosis of the tegumental syncytium were seen in all part of cysticercus. 2. The scanning electron microscopic findings of Cysticercus cellulosae obtained from the 4th ventricle of the cerebral cysticercosis patients by the surgical operation were identical with those of subcutaneous tissues. 3. In 12 cases of cerebral cysticercosis treated with prziquantel in dosage of 3×25 ㎎/㎏ for 3 or 4 consecutive days, there were no cessations of the convulsive seizures during the 6 months follow up. Among them 9 cases were given again with the same doses of the drug for 4 or 7 days. In 7 of 9 cases, no more convulsive seizure was experienced over one or two years after the second treatment. At the same time the lesions of the brain C.T. scanning were disappeared, decreased in size or calcified. In the other 3 cerebral cysticercosis cases, the complete cure was also obtained after the oral medication of praziquantel with the doses of 3×25 ㎎/㎏ for 7 consecutive days. 4. In the treatment of cerebral cysticercosis with praziquantel, it was found that the combined oral medication of dexamethasone during the course of treatment was effective on preventing and minimizing the side effects.