침 시술로 발생한 Non-O1, Non-O139 Vibrio cholerae에 의한 패혈증

임태섭,지아영,이중희,장수연,김인수,김영주,김범경,김승업,박준용,안상훈,한광협,김도영 Ewha Womans University School of Medicine 2013 EMJ (Ewha medical journal) Vol.36 No.S

Vibrio cholerae is mainly known to cause gastrointestinal infection after seawater exposure or raw seafood intake. It is rarely reported to cause cellulitis or sepsis, but threre has been no known case after acupuncture. Herein, We report a 56-year-old cirrhotic patient of non-O1, non-O139 Vibrio cholerae septicemia caused by cellulitis of both lower extremities after acupuncture.

치근단 병소의 임상적 연구

김상우,우순섭,유임학,이영수,심광섭 대한악안면성형재건외과학회 2001 Maxillofacial Plastic Reconstructive Surgery Vol.23 No.3

The purpose of this study was to find the clinical features of periapical lesions. A total of 130 periapical lesions which were obtained from biopsy and diagnosed histopathologically as periapical cyst, periapical abscess, and periapical granuloma at the Department of Dentistry in Hanyang University Hospital were throughly analysed according to the distribution and incidence of age, sex, location, and so on. The following results were obtained : 1.Out of 130 periapical lesions, 88 cases(67.7%) were periapical cysts, 30 cases(23.1%) were periapical abscesses, and 12 cases(9.2%) were periapical granulomas. 2.The periapical lesions occurred most frequently in the third decade, and followed by the fourth, fifth, sixth, and second decade. The periapical cysts occurred most frequently in the third decade(26.1%), the periapical granulomas in the fourth decade(33.3% ) the periapical abscesses in the sixth decade(26.7%). 3.The periapical lesions were more frequent in men than in women with the ratio of men to women of 1.4 : 1. The radio of men to women of periapical cysts was 1.6 : 1, that of periapical granulomas was 0.5 : 1, and that of periapical abscesses was 1.3 : 1. 4.The periapical lesions were more frequent in maxilla than in mandible with the ratio of 1.2 : 1. The ratio of maxilla to mandible of periapical cysts was 1.5 : 1, that of periapical granulomas was 0.5 : 1, that of periapical abscesses was 1 : 1. 5.The most commonly involved location of the periapical lesions was maxillary anterior teeth(40.8%), and followed by mandibular molars, mandibular premolars, and mandibular anterior teeth and maxillary molars. The most frequent location of the periapical cysts was maxillary anterior teeth(48.9%), that of periapical granulomas was mandibular molars(50.0%), that of periapical abscesses was mandibular molars(40.0%).

주변성 법랑모세포종의 증례

송주섭,김경아,고광준 대한구강악안면방사선학회 2006 Imaging Science in Dentistry Vol.36 No.2

Peripheral ameloblastoma is an extremely rare odontogenic soft tissue tumor with histologic characteristics similar to those of the intraosseous ameloblastoma. It appears in the gingiva and oral mucosa. And it usually does not show any bone involvement on radiographs, except for saucer shaped erosion of underlying alveolar bone. Recurrence is considered uncommon. We report a case of peripheral ameloblastoma with bone involvement. Histologically it presented with follicles and nest of tumor cells with palisading pattern. And radiographs showed the typical saucer shaped alveolar bone erosion at the distal area of right mandibular third molar. At 6-month follow-up after operation, no local recurrence was noted.

한국인 두경부암종 환자에서 Cytochrome P450 1A1,2E1 및 N-acetyltransferase 2 효소의 다형성 분석에 따른 유전적 감수성에 대한 연구

심광섭,김태균,우순섭,이영수,공구 대한악안면성형재건외과학회 2000 Maxillofacial Plastic Reconstructive Surgery Vol.22 No.4

Individual genetic susceptibilities to cancers may result from several factors including differences in xeno-biotics metabolism to chemical carcinogens, DNA repair, altered oncogenes and suppressor genes, and environmental carcinogen exposures. Among them, genetic polymorphisms of metabolizing enzymes to chemical carcinogens have been recognized as a major important host factors in human cancers. They have two main types of enzymes: the· phase I cytochrome P-450 mediating enzymes (CYPs) and phase Ⅱ conjugating enzymes. The purpose of this study is to determine the frequencies of genotypes of phase I (CYP1A1 and CYP2E1) and phase Ⅱ (NAT2) metabolizing enzymes in healthy control and head and neck cancer patients of Korean and to identify the relative high risk genotypes of these metabolizing enzymes to head and neck cancer in Korean. The author has analyzed 132 head and neck cancer patients and 113 healthy controls using polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) . The results were as following; 1.The frequencies of genotypes of CYP1A1, CYP2El and NAT2 in healthy control were as following; CYP1A1 exon 7 polymorphism; Ile / Ile: Ile/Val: Va1/Va1 = 59.3%: 36.3%: 4.4% CYP2El Pst I polymorphism, C1/Cl: C1/C2: C2/C2 = 61.1%: 32.1%: 6.2% NAT2 polymorphism; F/F: F/S: S/S = 43.4%: 48.7%: 8.0% 2.In analysis of phase I enzyme, Val/Val genotype in CYP1A1 exon 7 polymorphism and C2/C2 genotype in CYP2El Pst I polymorphism were associated with relative high risks to head and neck cancers (Odds' ratio: 2.09 and 1.37, respectively). 3.Among the genotypes of NAT2 enzyme polymorphism, S/S genotype of NAT2 enzyme had 1.03 times of relative risk to head and neck cancers. 4.In combined genotyping of CYP1A1, CYP2El, and NAT2 enzymes polymorphisms, the patients with Va1/Va1 and Cl/Cl, C2/C2 and fast acetylator, and Va1/Va1 and fast acetylator had higher relative risks than the patients with each baseline of combined genotypes (Odds' ratio: 2.82, 1.98 and 2.1, respectively) . These results suggest the combined genotypes of Va1/Va1 and Cl/Cl , C2/C2 and fast acetylator, and Val/Val and fast acetylator were more susceptible to head and neck cancers in Korean. And genotyping of metabolizing enzymes could be useful for predicting individual susceptibility to head and neck cancer.

Quinacrine 형광을 이용한 토마토 뿌리조직 마이크로솜의 수소이온이동 활성측정

신대섭,조광현,김영기 충북대학교 한국과학재단 지정 첨단원예기술개발 연구센터 2002 연구보고서 Vol.6 No.-

Quinacrine은 수소이온 농도변화에 민감한 형광 probe로서 양성자와 결합하지 않은 형광형이나, 양성자와 결합한 비형광형으로 존재한다. 따라서, quinacrine은 H+-ATPase에 의한 수소이온이동 활성측정에 이용된다. 본 연구에서는 토마토 뿌리조직에서 분리한 마이크로솜에서 quinacrine의 형광성을 이용한 H+-ATPase 활성측정의 최적 조건을 조사하였다. Quinacrine의 형광변화는 반응용액 중의 단백질 함량이 0.43㎍/㎕에서 25-26% 감소하여 10%의 quinacrine 형광을 감소시키는 데 약 100 nmol/min의 H+-ATPase 활성이 필요함을 알 수 있었다. Quinacrine의 최대 형광변화는 pH 7.0-7.2 범위와 2 mM Mg2+ 조건에서 일어났다. 이것은 기존에 보고한 H+-ATPase의 특성과 잘 일치하여, quinacrine의 형광변화가 H+-ATPase의 활성을 잘 반영하고 있음을 보인다. 원형질막 및 액포막 H+-ATPase들의 선택적 저해제인 vanadate와 NO3-는 각각의 효소에 의한 수소이온이동 활성을 저해하는데 성공적임을 확인하였다. 이상의 결과로 quinacrine이 토마토 뿌리조직에서 분리한 마이크로솜의 수소이온이동 활성측정에 유용하게 이용될 수 있음을 확인하였다. Quinacrine, a pH-sensitive fluorescence probe, which exists either as an unprotonated fluorescence form or protonated nonfluorescence form, can be used to measure the proton transport activity of H+-ATPase. Quinacrine was used to determine the optimal conditions for measuring the activity of microsomal H+-ATPases prepared from the roots of tomato plants. The amount of quinacrine fluorescence quenching obtained at 0.43 ㎍/㎕ of microsomal protein concentration was 25-26%, which shows that the enzyme activity of 100 nmol/min decreases 10% of quinacrine fluorescence. Maximal fluorescence quenching was obtained at pH 7.0-7.2 and 2 mM Mg2+. Because the activity of microsomal H+-ATPase is also maximal at these conditions, the quinacrine fluorescence well represents the activity of H+-ATPase. Vanadate and NO3-, specific inhibitors of plasma and vacuolar H+-ATPase, respectively, were successfully applied to inhibit the quinacrine fluorescence quenching mediated by the corresponding H+-ATPase. These results imply that quinacrine is a useful tool for measuring the proton transport activities of microsomes obtained from the root tissued of tomato plants.

Pendulum 장치의 상악대구치의 원심이동에 대한 효과

이창섭,김재광,강덕일,송광철,정현구,이상호 대한소아치과학회 2001 大韓小兒齒科學會誌 Vol.28 No.3

Ⅱ급 치성교합을 가진 환자에서는 I급 구치부 교합관계를 형성하기 위하여 상악 대구치의 원심이동이 필요하다 본 연구에서는 pendulum장치를 이용한 상악 대구치의 원심이동시 대구치의 이동양상과 고정원의 전방이동 및 정출정도 등 치아와 골격에 미치는 영향을 평가하기 위하여 시행되었다. 상악 제1대구치를 원심이동시켜 1급 구치관계가 형성된 19명의 환자 (남: 8명. 여: 11명, 평균연령 11.68±1.52세)에서 장치 장착 전과 장치제거 직후에 얻어진 측면두부방사선사진을 비교 ·분석하여 다음과 같은 결과를 얻었다. 1.I급 교합관계를 형성하기 위해서 제 1대구치는 평균 2.94± 1.54mm 원심이동하였으며 . 1.17±0.97mm 함입되었고, 소요기간은 평균 18.13±7.95일이었다. 2.고정 원으로 사용된 치아는 1.34± 1.40mm로 유의하게 전방 이동하였으며, 0.48±0.99mm 정출되었다. 또한 절치는 유의한 전방이동 및 순측경사가 발생하였다(P<0.05) . 3.구개평면과 하악평면이 이루는 각은 유의한 증가를 보였다(P(0.05). 4.제1소구치 또는 제1유구치가 고정 원으로 이용된 경우 제2소구치 또는 제2유구치가 이용된 경우 보다 전방이동이 증가하였다(P<0.005). 5. 제2대구치의 발육단계에 따른 제1대구치 및 제2대구치의 이동양상은 어떤 항목과도 유의성이 없었다(P<0.05). 6.얻어진 총 공간 중 74%는 제1대구치의 원심이동이,26%는 소구치의 전방이동에 의하여 얻어졌다. 이상의 결과를 종합하여 볼 때 pendulum appliance는 상악 대구치의 원심이동에 매우 효과적이었으며. 고정원의 소실을 최소화하기 위해 제2유구치 또는 제 2소구치가 포함되어야 한다. 또한 제1대구치의 원심경사는 필연적으로 일어나며. 이를 최소화하기 위한 노력이 계속되어야 할 것으로 사료된다. Treatment of class Ⅱ malocclusions require distalization of maxillary molars into class I relationship. Intra-arch distal molar movement techniques have recently assumed an important role in young patients. In this study, the dental and skeletal effects of the pendulum appliance were evaluated by means of cephalometric radi-ographs. The samples were consisted of 19 patients: 11 females and 8 males, mean age 11.68± 1.52 years. Measurements were obtained from cephalometric prior to and the day of removal of the pendulum appliance. Treatment changes were analyzed. The following results were obtain : 1. The pendulum appliance produced 2.94±1.54mm distal molar movement with a mean intrusion of 1 17± 0.97mm, mean period 18.13±7.95 weeks. 2. The anchor tooth was 1.34± 1.40mm forward movement and 0.48±0.99mm extrusion, and labial tilting of incisors. 3. The angle between palatal plane and mandibular plane increased significantly 4 There was no significant difference in according to 2nd molar position. 5. Total movement was consisted of 74% distal movement of 1st molar and 26% forward movement of the an-chor tooth

비균일 열유속하에서 압력강하 불안정성의 요동특성

申光燮,金秉宙 弘益大學校 科學技術硏究所 1993 科學技術硏究論文集 Vol.3 No.-

An experimental study on the oscilatory characteristics of pressure-drop oscillations (PDO) has been carried out in a non-uniformly heated boiling channel using freon-113 as the working fluid. The effects of initial and boundary conditions on PDO are investigated in terms of oscillation period and amplitude. Generally boiling channel under uniform heat flux showed higher amplitude and period of PDO than that of consine-type non-uniform heat flux. The period and amplitude of PDO are increased with the increase in the compressible volume in surge tank, heat input, and with the decrease in the initial flowrate of the fluid supply sectin. However the effects of inlet temperature of working fluid are very similar for both the uniformly and non-uniformly heated channels.

치주인대 세포의 보관방법에 따른 생활력에 관한 연구

현원섭,이긍호,김광철 大韓小兒齒科學會 1994 大韓小兒齒科學會誌 Vol.21 No.1

This study was designed to compare the ability of human periodontal ligament cell viability according to different storage methods, storage time and temperature, and to evaluate the osmolality and pH of the storage media. In the present investigation, media tested for their ability of PDL cell were HBSS, milk, milk contained antibiotics, saliva, ion drink, saline. Time interval was 2 min, 5 min, 10 min, 30 min, 60 min, 120 mi, and storage temperature was 4。C and room temperature. The viability of PDL cells stored for these media was determined by the trypan blue dye exclusion test. Viable (non-stained) and non-viable (stained) cells were counted in a hemocytometer. The pH of storage media were evaluated at 4。C and room temperature. The osmolality of the samples was analysed in an Osmometer, 3D-Ⅱ model. The results were as follows: 1. PDL cell viability was declined in the sequence of DMEM, HBSS, milk contained antibiotics, milk, saline, saliva, ion drink, Distilled water(p<0.01). 2. PDL cell viability was more depended on pH than Osmolality of storage media. 3. PDL cell viability was better at 4。C than room temperature(p<0.01). 4. PDL cell viability was decreased with time(p<0.01).

상악동거상술을 이용한 인공치아 이식 치험례

우순섭,장윤성,김태균,이영수,심광섭,유광희 한양대학교 의과대학 1999 한양의대 학술지 Vol.19 No.1

Recently many dentists would like to select the implant installation for the restoration of partially or fully edentulous patient. Usually, it is needed at least 8mm of alveolar bone height for implant installation. But, in many cases, the implant installation is impossible due to anatomic limitation such as maxillary sinus, floor of nose, mandibular canal, lack of width, etc. So, dentists must come over these limitations by methods of sinus lift, nerve repositioning, transmandibular implant etc. Especially, a lot of ridges have inadequate bone height at the upper molar edentulous areas, and to insert implant for such sites represents low success rate. So, autogenous or allogenic or alloplastic bone can be grafted before or at the same thme with implant installation. We report a case of 22 year-old woman who was performed implant installation successfully by using sinus lift technique with iliac block bone graft to a patient whose low alveolar bone height made common implantation impossible. Now, she exerts good occlusive function at the time of 15 months after the operation.

상수 고도처리에서 활성탄 재생기술

손성섭,김진오,최광호 嶺南大學校 環境問題硏究所 1999 環境硏究 Vol.18 No.2

요 약 국내 상수도는 취수원의 90%이상을 지표수에 의존하므로 하천오염에 따른 수원의 수질악화가 불가피하며, 또한 생활수준 향상에 따라 먹는물 수질기준의 강화가 예상되므로 상수고도처리의 도입이 절실한 실정이다. 오존처리와 활성탄 여과는 현재 가장 널리 적용되는 상수고도처리법으로서, 특히 활성탄 여과는 냄새, 맛, 색도 및 난분해성 유기물질을 흡착하는데 효과적이므로 국내 20여개 정수장에서 적용하고 있다. 활성탄 여과지에 사용되는 활성탄은 피흡착물에 의해 포화흡착된 후 신탄으로 교체하거나 재생공정을 거쳐 재사용하며, 재생 방법으로는 열재생, 용매추출, 생물학적 산화, 화학적 산화 등이 있으나 활성탄을 다량 사용하는 수처리에는 열재생법이 가장 경제적인 방법으로 평가된다. 열재생은 가열에 의해 흡착 유기물을 제거하는 것으로 건조, 탄화, 부활의 공정으로 진행되며, 재생설비로는 다단로, 로타리킬른, 유동층로, 이동층로 등을 고려할 수 있다. 국내에서의 활성탄 재생설비는 제당공장, 연구용 및 고도폐수처리 등에서 일부 사용되고 있으며 상수고도처리 분야에서는 국내 몇 개 정수장에 적용하여 시공단계에 있지만 수질오염의 심각성 및 시설의 경제성을 고려할 때 그 수요가 점차적으로 증가될 것으로 예상된다.