http://chineseinput.net/에서 pinyin(병음)방식으로 중국어를 변환할 수 있습니다.
변환된 중국어를 복사하여 사용하시면 됩니다.
출아효모에서 TAR 클론닝법을 이용한 고등동물의 게놈으로부터 특정 염색체 부위의 분리
박정은,윤영호,이윤주,김광섭,윤세련,안태진,임선희,선우양일 동아대학교 기초과학연구소 2003 基礎科學硏究論文集 Vol.20 No.1
복잡한 게놈 분석에 용이하도록 효모의 인공 염색체(YAC) 클론닝 시스템은 발달되어왔다. YAC은 박테리아의 인공 염색체(BAC)보다 더 큰 단편을 클론닝할 수 있고, 또한 클론된 단편을 쉽게 변형시킬 수 있다. 형질전환과 연계된 재조합법(Transformation-Associated Recombination; TAR)은 게놈 라이브러리를 만들지 않고 직접 게놈 DNA로부터 분리하고자 하는 유전자나 특정 염색체 부분을 클론닝 할 수 있는 방법이다. 이 방법은 spheroplast transformation을 수행하는 동안, 목적으로 하는 유전자의 5' 그리고 3' 염기 배열(hooks)을 지닌 TAR 벡터와 게놈 DNA 사이에서 일어나는 상동성 재조합에 의해 이루어진다. 효모 내의 in vivo 재조합을 이용한 TAR 클론닝법은 복잡한 게놈으로부터 목적의 염색체 부분을 원형 YAC의 형태로서 선택적으로 분리할 수 있다. 그러므로 TAR 클론닝 법은 특정 염색체로부터 YACs을 만드는데 매우 유용하여, 전체 게놈으로부터 특이적 유전자나 유전자의 family를 분리하는데 효과적인 방법으로 사료된다. Yeast artificial chromosome (YAC) cloning systems have advanced the analysis of complex genomes considerably. They permit the cloning of larger fragments than do bacterial artificial chromosome systems, and the cloned material is more easily modified. Transformation-associated recombination (TAR) is a cloning technique that allows specific chromosomal regions or genes to be isolated directly from genomic DNA without prior construction of a genomic library. This technique involves homologous recombination during spheroplast transformation between genomic DNA and a TAR vector that has 5' and 3' gene targeting sequences (hooks). Using in vivo recombination in yeast, TAR cloning selectively isolates, as circular YACs, desired chromosome segments or entire genes from complex genomes. We propose that TAR cloning can provide an efficient means for generating YACs from specific chromosomes and that TAR cloning may be useful for isolating families of genes and specific genes from total genome DNA.
아가리쿠스버섯에서 분리한 조단백다당류의 막분리 및 분무건조 특성
홍주헌,윤광섭,최용희 한국식품저장유통학회 ( 구 한국농산물저장유통학회 ) 2004 한국식품저장유통학회지 Vol.11 No.1
η_(evap)분자량에 따라 다양한 생리활성을 나타내는 아가리쿠스버섯에서 분리한 조단백다당류를 고부가가치 기능성 소재로 개발하기 위하여 막분리 및 분무건조 공정에 따른 특성을 조사하였다. 아가리쿠스버섯 조단백다당류를 분자량 크기에 따라 여과하는 동안 공정온도에 따른 투과플럭스의 변화는 10 kDa와 150 kDa 모두에서 유사하였는데, 막분리 온도 및 압력이 증가함에 따라 비례적으로 증가하였으며,40℃에서의 투과플럭스가 가장 높았다. 시간에 따른 투과플럭스의 변화는 막분리 30분 경과시 급속히 감소하였으며, pore size가 10 kDadhk 150 kDa일 때 공정압력이 각각 1.2 kg_(f)/㎠ 와 2 kg_(f)/㎠에서 가장 높았다. 한외여과 후 아가리쿠스버섯 조단백다당류의 점도는 여과 전 13.9cP에서 여과후 10 kDa이하와 10∼150 kDa구간은 각각 10.8, 11.9cP로 점도가 저하되었으나, 150 kDa이상은 20.1cP로 점도가 증가되었다. 분자량 크기가 다른 3가지 분획물에 대한 분무건조 공정에서의 열효율성은 가열공기온도, 시료공급속도 및 농도가 증가할수록 열효율성이 증가함을 알 수 있었으며, 시료공급속도가 가장 중요한 영향인자임을 확인하였다. This study was conducted to investigate the characteristics of ultrafiltration and spray drying process for crude protein bound polysaccharide(CPBP) isolated from Agaricus blasei Murill. In ultrafiltraion process, the permeate flux increased with the increase of operating pressure and temperature. The permeate flux declined continuously while the fouling materials were accumulated on the membrane as the operation time increased. In comparing of raw CPBP and filtered CPBP, the viscosity of CPBP treated UF was decreased and AE value of treated samples was increased. Thermal efficiencies of spray drying process were increased by increasing inlet temperature, feed rate and feed concentration.
우순섭,장윤성,김태균,이영수,심광섭,유광희 한양대학교 의과대학 1999 한양의대 학술지 Vol.19 No.1
Recently many dentists would like to select the implant installation for the restoration of partially or fully edentulous patient. Usually, it is needed at least 8mm of alveolar bone height for implant installation. But, in many cases, the implant installation is impossible due to anatomic limitation such as maxillary sinus, floor of nose, mandibular canal, lack of width, etc. So, dentists must come over these limitations by methods of sinus lift, nerve repositioning, transmandibular implant etc. Especially, a lot of ridges have inadequate bone height at the upper molar edentulous areas, and to insert implant for such sites represents low success rate. So, autogenous or allogenic or alloplastic bone can be grafted before or at the same thme with implant installation. We report a case of 22 year-old woman who was performed implant installation successfully by using sinus lift technique with iliac block bone graft to a patient whose low alveolar bone height made common implantation impossible. Now, she exerts good occlusive function at the time of 15 months after the operation.
( Youn Kyung Choi ),( Jung-il Kang ),( Jin Won Hyun ),( Young Sang Koh ),( Ji-hoon Kang ),( Chang-gu Hyun ),( Kyung-sup Yoon ),( Kwang Sik Lee ),( Chun Mong Lee ),( Tae Yang Kim ),( Eun-sook Yoo ),( H 한국응용약물학회 2021 Biomolecules & Therapeutics(구 응용약물학회지) Vol.29 No.2
Alopecia is a distressing condition caused by the dysregulation of anagen, catagen, and telogen in the hair cycle. Dermal papilla cells (DPCs) regulate the hair cycle and play important roles in hair growth and regeneration. Myristoleic acid (MA) increases Wnt reporter activity in DPCs. However, the action mechanisms of MA on the stimulation of anagen signaling in DPCs is not known. In this study, we evaluated the effects of MA on anagen-activating signaling pathways in DPCs. MA significantly increased DPC proliferation and stimulated the G2/M phase, accompanied by increasing cyclin A, Cdc2, and cyclin B1. To elucidate the mechanism by which MA promotes DPC proliferation, we evaluated the effect of MA on autophagy and intracellular pathways. MA induced autophagosome formation by decreasing the levels of the phospho-mammalian target of rapamycin (phospho-mTOR) and increasing autophagy-related 7 (Atg7) and microtubule-associated protein 1A/1B-light chain 3II (LC3II). MA also increased the phosphorylation levels of Wnt/β-catenin proteins, such as GSK3β (Ser<sup>9</sup>) and β-catenin (Ser<sup>552</sup> and Ser<sup>675</sup>). Treatment with XAV939, an inhibitor of the Wnt/β-catenin pathway, attenuated the MA-induced increase in β-catenin nuclear translocation. Moreover, XAV939 reduced MA-induced effects on cell cycle progression, autophagy, and DPC proliferation. On the other hand, MA increased the levels of phospho (Thr<sup>202</sup>/Tyr<sup>204</sup>)-extracellular signal regulated kinases (ERK). MA-induced ERK phosphorylation led to changes in the expression levels of Cdc2, Atg7 and LC3II, as well as DPC proliferation. Our results suggest that MA promotes anagen signaling via autophagy and cell cycle progression by activating the Wnt/β-catenin and ERK pathways in DPCs.
Hybrid Robust Controller Design for a Two Mass System with Disturbance Compensation
Kwang-Ho Yoon,Jong-Kwang Lee,Ki-Ho Kim,Byung-Suk Park,Ji-Sup Yoon 제어로봇시스템학회 2008 제어로봇시스템학회 국제학술대회 논문집 Vol.2008 No.10
In this paper we describe a robust control method for when a torque or inertia change in a two mass system. There are various controllers that represent a robustness. Especially, the disturbance observer (DOB) and H ∞of them are well known for their efficiency with respect to a disturbance rejection. Actually, there is method to make use of two controllers together as a cascade type. But the performance of this control is not efficient due to a fault in that the system degree increases when it designs a controller. In this paper, a hybrid type disturbance compensation is proposed for robust control methods. Basically, a hybrid robust controller is driven by the H∞ H controller, and a H∞ H control of the limited scope of a control for a disturbance when compared with the disturbance observer (DOB) will be compensated for by the H∞ H control input. We performed a DOB and H∞ H comparison simulation to prove the performance of the controller that is proposed in this paper. We performed simulations for a change in the torque and inertia and found that the proposed controller showed a small error. For a verification, we performed simulations by using MATLAB/SIMULINK.
Design of a Servomanipulator with Tendon Transmission
Jong Kwang Lee,Chang Hwan Choi,Kwang Ho Yoon,Byung Suk Park,Ji Sup Yoon 제어로봇시스템학회 2008 제어로봇시스템학회 국제학술대회 논문집 Vol.2008 No.10
This paper describes a master-slave servomanipulator with a tendon transmission, which was designed to overcome the limited workspace of the conventional mechanical master-slave manipulators. The tendon transmission greatly reduces the weight and friction, and it also allows us to realize a zero-backlash reduction. However, it causes a motion coupling problem due to an unwanted motion interference at the joints. In this work, we solve this problem through a novel motion decoupling mechanism.
윤경섭 ( Yoon Kyung Sup ),이광식 ( Lee Kwang Sik ),김형중 ( Kim Hyung Joong ),김호일 ( Kim Ho Il ) 한국농공학회 2003 韓國農工學會誌 : 전원과 자원 Vol.45 No.4
Monitoring data from agricultural reservoirs throughout the country were analyzed to classify agricultural reservoirs according to physical characteristics and COD concentrations, and evaluate the relationships bet-ween water quality items. The physical and chemical data of total 498 reservoirs were analyzed from 1990 to 2001. Average COD, TP, TN, Chl-a, SS concentrations for the reservoirs and pollutant loadings from their watersheds were used for the analysis. It was possible that reservoirs were classified to 4 types using the relationships between the ratios of effective storage per water surface (ST/WS ratio) and COD concentrations. It is recommended that the improvement measures of polluted reservoirs should be performed as following order : integrated consolidation type (complex mechanism type) → watershed consolidation type → integrated consolidation type (external mechanism type) → in-lake consolidation type → conservation type and the depth (ST/WS ratio) of reservoir maintained over 5~6 m for water quality improvement. The decision coefficients (r<sup>2</sup>) between Chl-a and other items (COD, T-P, SS, T-N) were 0.6915, 0.6732, 0.5327, 0.3352, respectively. Therefore, reservoir managers could evaluate the trophic state of reservoirs by COD concentrations.