해바라기(Helianthus annus L.) 배양세포의 Cellular Peroxidase 활성을 억제하는 비병원성 Elicitor의 확인

송지연,신광숙,이인철 대전대학교 기초과학연구소 1999 自然科學 Vol.10 No.2

효모(Sacharomyces cerevisiae)의 세포벽 성분은 가장 잘 알려진 nonpathogenic elicitor이다. 이런 종류의 elicitor가 식물세포의 생리적 변화를 유도하는 기작을 규명하기 위하여 해바라기(Helianthus annus L.)의 배양 세포에 투여한 후 peroxidase 활성 변화를 측정하였다. 선행된 실험에서 보여주는 바 장미나 담배의 배양세포에서 yeast-elicitor는 peroxidase 의 활성, 특히 extracellular peroxidase 의 활성을 증가시켰으며, 기존의 peroxidase 와 구분되는 isoperoxidase 의 de novo synthesis를 유도한다는 증거를 얻은 바 있다. 그러나 해바라기 배양세포에서는 동일한 eliciting에 대하여 extracellular peroxidase의 활성변화는 관찰되지 않았으며 cellular peroxidase가 오히려 감소되는 결과를 얻었다. 효모 세포벽 성분을 DEAE-cellulose, CM-sepharose 및 concanavalin A-sepharose chromatography를 통하여 분획한 결과 DEAE-celluose로부터 용출된 분획과 concanavalin A로부터 용출된 분획이 peroxidase 의 활성을 억제하였으며 이러한 억제는 시료를 투석하여 glucan의 농도가 감소하는 것에 비례적으로 감소하였다. 그러므로 상기와 같은 반응을 유도하는 성분은 oligosaccharides라고 판단되었다. 이러한 결과는 동일한 elicitor에 대하여 식물의 종에 따라 반응하는 양식이 다름을 보여주는 결과라고 생각된다. Cell wall components of yeast (Sacharomyces cerevisiae) is well known nonpathogenic elicitor in some plant. To identify the chemical properties of yeast elicitor, the elicitor was isolated by ethanol precipitation followed by DEAE-cellulose, CM-sepharose and concanavalin A-sepharose chromatography. In contrast to the effect of elicitor in cultured cells of rose or tobacco, same elicitor from yeast has been found to suppress the peroxidase activity in cultured sunflower(Helianthus annus L.) cells. The inhibitory elicitor on peroxidase activity was detected in fractions eluted from DEAE-cellulose and from concanavalin A-sepharose column. The inhibitory effect of DEAE-fraction, however, was decreased by dialysis against distilled water using dialysis tubing with molecular cut off 1,000 Da. which suggested the yeast elicitor might be a oligosacharide. These results showed that plant might respond in different physiological ways to the same elicitor according to the plant species.

악교정 수술 후 하악과두의 위치 및 골변화에 관한 단층방사선학적 연구

송남규,고광준,김민숙 大韓口腔顎顔面 放射線學會 1992 Imaging Science in Dentistry Vol.22 No.2

The purpose of this study was to aid in the evaluation of prognosis of temporomandibular joint following orthognathic surgery. For this study, 20 patients(40 TMJ) who undergone orthognathic surgery were examined. Preoperative and postoperative tomograms of TMJ were taken. And the subjects were divided into 3 groups according to postoperative periods. The obtained results were as follows ; 1. There were no significant differences between preoperative and postoperative changes in joint spaces in each group and between groups(p<0.05). 2. There were no significant differences between preoperative and postoperative ratio of joint space(p<0.05). 3. There were no significant differences between preoperative and postoperative changes in the range of motion of condylar head(p<0.05). 4. The bony changes of condylar head were observed in 14(35%) condyles(6 erosion, 2 flattening, 5 double contour, 1 osteophyte).

전산화단층상을 이용한 안면골의 3차원재구성상의 비교 연구

송남규,김민숙,고광준 全北大學校 齒醫學硏究所 1992 전북치대논문집 Vol.10 No.1

The purpose of this study was to evaluate the spatial relationship of facial bone more accurately. For this study, the three-dimensional images of dry skull were reconstructed using computer image analysis system and three-dimensional reconstructive program involved CT. The obtained results were as follows : 1. Three-dimensional reconstructive CT results in images that have better resolution and more contrast. 2. It showed good marginal images of anatomical structure on both three-dimensional CT and computer image analysis system, but the roof of orbit, the lacrimal bone and the squamous portion of temporal bone were hardly detectable. 3. The partial loss of image data were observed during the regeneration of saved image data on three-dimensional CT. 4. It saved the more time for reconstruction of three-dimensional images using computer image analysis system. But, the capacity of hardware was limited for inputting of image data and three-dimensional reconstructive process. 5. We could observe the spatial relationship between the region of interest and the surrounding structures by three-dimensional reconstructive images without invasive method.

악교정 수술 후 하악과두의 위치 및 골변화에 관한 단층방사선학적 연구

송남규,김민숙,고광준 全北大學校 齒醫學硏究所 1992 전북치대논문집 Vol.10 No.1

The purpose of this study was to aid in the evaluation of prognosis of temporomandibular joint following orthognathic surgery. For this study, 20 patients(40 TMJ) who had undergone orthognathic surgery were examined. Preoperative and postoperative tomograms of TMJ were taken. And the subjects were divided into 3 groups according to postoperative periods. The results were as follows ; 1. There were no significant differences between preoperative and postoperative changes in joint spaces in each group and between groups(P<0.05). 2. There were no significant differences between preoperative and postoperative ratio of joint space(P<0.05). 3. There were no significant differences between preoperative and postoperative changes in the range of motion of condylar head(P<0.05). 4. The bony changes of condylar head were observed in 14(35% ) condyles(6 erosion, 2 flattening, 5 double contour, 1 osteophyte).

Mandibular overclosed patient를 위한 oral rehabilitation

송창용,김광석,이상돈,백희선,박찬운 全北大學校 齒醫學硏究所 1985 전북치대논문집 Vol.3 No.1

소위 정상 한국인에 있어서 General Health Questionnaire의 인자분석에 관한 연구

신석철,송광선 충남대학교 의과대학 지역사회의학연구소 1984 충남의대잡지 Vol.11 No.2

This investigation was attempted to observe the differences of the structure of the neurotic symptoms in normal persons. The number of the persons to be participated for this study was two hundred(105 males and 95 females). and was classified into four age-groups: 20 to 34, 35 to 44, 45 to 64, and over 65 years. The data of each group got from the method of the general health questionnaire (GHQ), and the scores were performed with the principal component factor analysis and Varimax-rotated factors were interpreted with the Kaiser normalization. The results were obtained the priority order of each factor in the neurotic symptoms as follows: 1. Seven factors in the age-group of 20 to 31 were hopelessness, headache, hypochondriasis, indecision, insomnia, helplessness, and inefficiency. 2. Four factors in the age-group of 35 to 41 were hopelessness, insomnia, inefficiency, and irritability. 3. Six factors in the age-group of 45 to 64 ware inefficiency, hopelessness, hypochondriasis, irritability, headache, and insomnia. 4. Four factors in the age-group of over 65 were hopelessness, inefficiency, hypochondriasis, and irritability. From the above results, the popular and dominant factors in all age-groups were hopelessness and inefficiency, the first factor in all-groups was hopelessness except the group of 45 to 64 and the first factor in the age-group of 45 to 64 inefficiency.

부력저항용 영구앵커의 거동

홍원표,송영식,고광태 중앙대학교 기술과학연구소 2002 기술과학연구소 논문집 Vol.32 No.-

본 연구에서는 부력저항용 영구앵커의 거동을 시공단계별로 구분하여 분석한다. 즉, 앵커의 두부에 하중계를 설치하여 앵커축력을 계측하였으며, 이 계측결과를 토대로, 앵커축력의 손실, 건물자중의 증가 및 외적조건 변화에 따른 앵커의 거동을 분석하였다. 영구앵커는 초기인장력에 비하여 Jacking Free시 인장력이 약 7% 정도 감소하며, 최종인장력은 약 15%정도 감소한다. 한편, 앵커의 초기인장력은 외력이 작용하지 않아도 PC 강선의 relaxation, 주입재 및 지반의 creep 등의 내적요인에 의하여 비선형적으로 감소되어 1차 수렴치에 도달하게 된다. 1차 흙되메우기 작업 기간동안 앵커축력은 흙되메우기에 따른 횡방향 토압이 증가 및 상부구조물의 자중증가로 인하여 감소된다. 그러나, 흙되메우기 작업이 중단된 기간동안 되메우기한 흙의 이완현상으로 횡방향 토압이 감소되며 이로 인하여 앵커축력이 증가하게 된다. 그리고, 되메우기한 흙속으로 지하수가 유입되어 부력이 증가하게 되므로 앵커축력은 증가하게 된다. 이때 앵커축력은 1차 수렴치까지 회복하게 된다. The behavior of permanent anchors for buoyant resistance is investigated at each construction stage. The prestressed anchor forces were measured by load cells which were attached to anchor head. On the basis of instrumentation, the loss of anchor force and the behavior of anchor by increment of structure weight and change of external condition were investigated. Prestressed anchor force at jacking free is decreased by about 7% than initial prestressed anchor force and decreased by about 15% finally. Although external forces does not act on the substructure, initial anchor force is decreased nonlinearly due to relaxation of PC strand and creep of grouting materials and soils. Also, anchor force was converged at the first convergent value. When first backfill was performed, anchor force was decreased due to increment of lateral earth pressure and superstructure weight. But, anchor force grew gradually during the period of breaking backfill by lowering of lateral earth pressure due to relaxation of soils. When groundwater inflowed into backfill soils, anchor force is slowly increased by uplift force below mat foundation. At that time, anchor force is recovered by the first convergent value.

입원 중인 베트남 참전 군인의 삶의 만족도와 관련된 요인

김애리,박종,김율,노희송,박광희,김은숙,김형철,홍강식,류소연,강명근,김기순 朝鮮大學校 附設 醫學硏究所 2007 The Medical Journal of Chosun University Vol.32 No.2

Background and Objectives: The aim of the study was to identify the factors associated with life satisfaction of hospitalized Vietnam veterans. Material and Methods: The data were collected from 200 hospitalized Vietnam veterans of 5 veterans hospitals in Korea from September 13 to 23, 2004. Information of general characteristics, health behavior, disease history, family function, depression and life satisfaction were collected by structured questionnaire. Results: The mean of life satisfaction was 21.48(±6.03), and the mean of depression was 53.10(±9.64). The prevalence of depression was mild depression 24.0%, moderate depression 43.5% and severe depression 25.0%. Depression was associated with life satisfaction and depression was higher, life satisfaction was lower. Family function and age were positively associated with life satisfaction, Conclusion: Life satisfaction was affected by depression and family function in hospitalized Vietnam veterans. Therefore, it was thought that psychosocial approaches such as improve depression and family function in care of hospitalized Vietnam veterans is required.

초등학생들의 물질 개념에 관한 연구

김석중,남철우,김정길,송판섭,한광래,최도성,김숙희 光州敎育大學校 初等敎育硏究所 2002 初等敎育硏究 Vol.17 No.-

Over the past 20 years, research on students' conceptual development of science concepts has begun to inform educators about the roles that prior knowledge and experiences play in the development of conceptual understandings. Whereas many initial studies focused on identifying and cataloging naive of misconceptions, more recent research has focused on how student concept development research can inform teaching practice and curriculum design. In this study, we investigated children's (ages 11∼12) spontaneously constructed or naive understanding of the particulate nature of matter prior to any formal instruction in the domain. Thirty students were interviewed concerning their understanding of the macroscopic and microscopic properties of the states of matter(solid, liquid, and gas), as well as their macro/microscopic understanding of phase changes and dissolving. Children expressed ideas about states of matter which were categorized as macrocontinuous, macroparticulate, or microparticulate. Furthermore, a substantial number of the children provided explanations of properties and processes which were consistent with those beliefs. These children's beliefs about matter were not fully and consistently developed across the spectrum of substances from continuous solids to particulate solids to liquids to gases. We speculate that children first develop local frameworks particular to different classes of substances and then slowly expand these frameworks to include a wide range of substances and their properties as well as such processes as melting and freezing.

일 대학 병원 마취 · 회복실 간호사의 간호활동 분석

강윤경,김경미,김연이,박혜옥,서광희,송숙녀,이현숙,조의영 대한간호행정학회 2006 간호행정학회지 Vol.12 No.1

Purpose: This study was aimed to specify roles of nurses from the anesthesia and recovery room by analyzing nursing activities as well as anesthesia nursing during surgery. Method: The objects were 12 RNs working in the recovery room of a university hospital located in Incheon. Self-report was performed by measuring stop-watch for five days from May 17th to May 21st in 2004. Research method was designed to record the time and frequency of the nursing activity from anesthesia and recovery room based on nurses' statement and other references for five days. The data were analyzed with descriptive statistics, ANOVA, and t-test via SPSS Win 10.0 program. Result: Nursing activities in anesthesia recovery room were classified into two different characteristics such as direct and indirect nursing activity. The activities consisted of 11 direct nursing areas and 39 nursing activities in anesthesia preparation room. The indirect nursing was classified into 8 nursing areas and 32 nursing activities. The direct nursing was classified into 12 nursing areas and 55 nursing activity. Also, the indirect nursing was classified into 7 nursing areas and 21 nursing activities in recovery room. In terms of prevalence of nursing activities in the anesthesia preparation room, observation and cooperation of anesthesia was the most prevalent activity, drug and eqipment management the second prevalent, and drug administration the third. On the other hand, in the recovery room, the most prevalent activity was vital sign checking and observation, the second most prevalent activity informative activity, and the third body temperature control. Nursing activity time was recorded according to the nursing characteristics. In the anesthesia preparation room, the direct nursing spent for 8092.20 minutes was larger than the indirect nursing spent for 7198.50 minutes. Also, in the recovery room, the direct nursing spent for 2361.16 minutes was larger than the indirect nursing spent for 1134.13 minutes. 4. Nursing activity time was compared to duty shifts. In the anesthesia preparation room, the direct nursing was more prevalent on day shift and the indirect nursing was prevalent on evening shift. However, in the recovery room, both direct and indirect nursing activities were prevalent on day shift. Conclusion: The role of anesthesia and recovery room nurses was analysed according to the time, frequency, and its characteristics.