당뇨병 백서의 간세포에서 Glucokinase 활성도 및 유전자 발현에 대한 인슐린의 영향

강성이,팽정령,서광식,안규정,우정택,김성운,양인명,김진우,김영설,김광원,최영길 경희대학교 유전공학연구소 1993 遺傳工學論文集 Vol.5 No.-

목적 당대사의 조절 상태에 따른 생체 변화를 분자 수준에서 이해하고자 식이 조건을 달리한 정상 백서와 화학적으로 유도된 당뇨병 백서의 간조직에서 혈당수준과 인슐린치료 정도에 따라 나타나는 글루코키나제 활성도 및 유전자 발현을 분석하였다. 방법 스트렙토조토신 정맥투여 후 당뇨병의 유발을 확인하고, 인슐린을 1일 3회 3일간 복강내로 투여하여 상태를 안정시킨 후, 인슐린 투여군은 인슐린 투여 6시간이내에 그리고 인슐린 비투여군은 24시간 후 단두하여 채혈하고 복강을 열어 간조직을 채취하였다. 채취한 간조직에서 글루코키나제 활성도는 인산화된 포도당에서 NADH의 형성을 형광분광계로 측정하였으며, 글루코키나제 유전자 mRNA발현은 Northern 분석법을 이용하였다. 성적 정상 백서에서 공복상태와 식이를 섭취한 경우에 간조직의 글루코키나제 효소의 활성은 차이가 없었으나, 글루코키나제 유전자 mRNA 발현은 증가되었다. 당뇨병이 유발된 백서의 간조직에서 글루코키나제 효소의 활성 및 글루코키나제 유전자의 mRNA 발현은 정상 백서에 비하여 낮았다. 인슐린 투쳐 후 글루코키나제 효소의 활성 및 글루코키나제 유전자의 mRNA 발현이 증가되었고, 특히 혈당이 정상화된 경우에서 글루코키나제 유전자의 mRNA 발현이 증가도었다. 결론 인슐린에 의한 간조직에서 글루코키나제 효소의 활성 및 글루코키나제 유전자의 mRNA을 증가를 볼수 있었다. 당뇨병 백서에서 인슐린 투여 후에 혈당조절이 안된 경우 간조직의 글루코키나제 유전자의 mRNA 발현이 증가가 없는 것으로 보아 글루코키나제 mRNA의 발현에는 인슐린 이외의 다른 요소가 관여할 것으로 생각된다. The liver-specific hexokinase isoenzyme, referred to as glucokinase, is thought to play a key reglulatory role in hepatic glucose metabolism. The glucokinase gene is, therefore, of interest both because of its tissue-specific expression and because of the several regulatory processes that can be analyzed. The level of hepatic glucokinase activity appears to be determined essentially by regulation of the rate of enzyme synthesis, with insulin playing a leading role as an inducer. We investigated the role of insulin for the induction of glucokinase in the liver of diabetic rats. Experimental diabetes was induced by injection of streptozotocin 7 days before the experiment. Regular insulin was given by three days intraperitoneal injection at 8-h interval. The glucokinase mRNA in the liver was estimated by Nothern blot assay, as well as by fluorometric enzyme activity assay. Glucokinase activity was not reduced in the liver of normal fasting rats as compared to normal fed rats. And glucokinase activity was reduced in the liver of diabetic rats as compared to normal rats. In diabetic rats treated with insulin, glucokinase enzyme activity were increased. But glucokinase mRNA expression was only increased in normoglycemic diabetic rat with treated with insulin as compared to hyperglycemic rat. These data indicate that insulin stimulates hepatic glucokinase enzyme activity and mRNA expression. But other hormonal or metabolic factors may be contribute to regulation of glucokinase mRNA expression.

HPLC를 이용한 Alnus속 식물에서의 diarylheptanoids의 정량분석

정동욱,김준식,조수민,이연아,김광호,김세원,이민원 중앙대학교 약학연구소 1999 약학 논총 Vol.13 No.-

Quantitative determination of diarylheptanoids (oregonin and hirsutanonol), which were characteristic components of Alnus spp. has been conducted by High Performance Liquid Chromatography. The HPLC analysis was carried out using an YMC J'sphere ODS-H80 column [150×4.6mm, S-4μm. 80A(particle)] and a combination of acetonitrile and H2O was used as mobile phase in gradient solvent system. The results showed differnce in the species (Alnus japonica, A hirsuta and A. hirsuta var. sibirica)

유명산의 식물상 및 산림군락분류에 관한 연구

김흥식,김인수,홍금희,김남영,박광서,김정유,박완근 江原大學校 森林科學硏究所 2003 Journal of Forest Science Vol.19 No.-

2002년 3월부터 2003년 10월까지 유명산 자연휴양림을 대상으로 식물상과 산림군락구조를 조사한 결과, 관속식물이 98과 331속 570종 1아종 81변종 4품종 2교잡종으로 총 658종류로 나타났으며, 한국특산식물은 10과 14속 13종 2변종 1교잡종 총 16종류. 희귀 및 멸종위기식물은 9과 10속 11종 총 11종류, 귀화식물은 12과 22속 28종 총 28종류로 나타났다. 산림군락은 신갈나무-철쭉군목, 신갈나무-생강나무군단, 소나무-산거울군락, 잣나무-주름조개풀군락, 물푸레나무-신갈나무군락, 층층나무-신갈나무군락, 굴참나무-신갈나무군락, 싸리-신갈나무군락으로 전체 1군목, 1군단, 6군락이 분포하는 것으로 나타났다. The study was carried out to investigate the flora and community classification of forest vegetation in Mt. Yumyeong from March, 2002 to October, 2003. As a result of that, the vascular plants were 98 families, 331 genera, 570 species. 1 subspecies, 81 varieties, 4 formae, 2 hybrid, 658 taxa in all. Korean endemic plants were 10 families, 14 genera, 13 species, 2 variaties, 1 hybrid. 16 taxa in all ;, rare and endangered plants were 9 families, 10 genera. 11 species, 11 taxa in all ; naturalized plants were 12 families, 22 genera, 28 species, 28 taxa in all. The forest vegetation was classified into 1 order, 1 alliance. 6 communities : Rhododendro - Quercetalia mongolicae (Kim, 1990) Lindero - Quercion mongolicae (Kim, 1990) A. Carex humilis var. nana - Pinus densiflora Community B. Oplismenus undulatifolius - Pinus koraiensis Community C. Fraxinius rhynchophylla - Quercus mongolica Community D. Cornus controversa - Quercus mongolica Community E. Quercus variabilis - Quercus mongolica Community F. Lespedeza bicolor - Quercus mongolica Community

배·급수 시스템에서 부식방지제의 적용 평가 연구

우달식,문정기,구성은,최종헌,김주환,문광순 대한상하수도학회 2003 상하수도학회지 Vol.17 No.5

This study evaluated the effect of the corrosion inhibitors on the corrosion control performance in drinking water distribution system. A phosphate-zinc based inhibitor formulated was for the efficient corrosion control in drinking water distribution system. In a continuous-circulation test, 99% reduction in corrosion rate was achieved. The optimum concentration of the corrosion inhibitor was found to be 2.2㎎ PO₄/L. The effect of the corrosion inhibitor was significant for the pipes of carbon steel, galvanized steel and copper, but less for that of stainless steel. The corrosion inhibitor can be an effective cure for corrosion and red water problems by prevention the old pipes from further corrosion. Tap water in Mokpo and Kwangyang were more corrosive than the water in the rest of 4 other regions(Seoul, Pohang, Pusan, Puyeo). Tap water in Pusan was high in various ionic concentrations in general. The corrosion inhibitor reduced the corrosion rate by 76-94%, depending on the origin of the tap water.

누설전류 파형 측정에 의한 실리콘 고무의 표면 열화 평가

김정호,정규원,서광석,유영식,박용관 成均館大學校 科學技術硏究所 1999 論文集 Vol.50 No.1

In this study, we investigated the erosion resistance of the silicone rubber by measuring leakage current with Inclined-Plane Method. From the results of our study, it was observed that the typical leakage current waveform was the mixed figure of sinusoidal wave and rectifying wave. And, it is considered that the former is due to conductivity of the contaminant when the surface is wet, and the latter is due to the arc discharge of dry band. And, as the surface erosion is being accelerated, the proportion of the rectifying wave is increasing. And, the ratio of discharge duration to discharge-extinguish time changes and can be divided into three regions, which is similar to the changes of erosion depth and width. Therefore, it can be known that the erosion process has some correlation with the change of leakage current.

부신절제후의 위점막 점액세포의 미세구조

고정식,양남길,구광일,안의태,박경호 순천향대학교 1989 논문집 Vol.12 No.3

The experiment was conducted on the male Sprague-Dawley rats weighing about 250g each, to study the fine structural changes of the gastric mucus-secreting cells following bilateral adrenalectomy. Adrenalectomy was performed by dorsal approach method under ether anesthesia. Animals are fed ad libitum after the operation. The normal and the adrenalectomized rats were sacrificed on the 1st, 3rd, 5th and 7th day after the operation. For the electron microscopic observation, gastric mucosae were fixed in 2.5% glutaraldehyde-1.5% paraformaldehyde followed by post fixation in 1% osmium tetroxide. The ultrathin sections were contrasted with uranyl acetate and lead citrate, and examined with JEM 100CX Ⅱ electron microscope. Obtained results were follows: 1. The gastric surface mucous cells of adrenalectomized rat contained mucous granules of less electron density than those of the normal rat contains. And mucous granules of adrenalectomized rats are usually placed apart from the surface plasma membrane. 2. Goblet cells of adrenalectomized rat exhibited well formed microvilli as compared with those of normal rat. 3. Cell differentiation in the isthmic area was generally seem to be delayed following adrenalectomy. 4. Golgi complexes of goblet cells were influenced by adrenalectomy, showing flattening or disorganization of cisterns, non-typical vacuoles, scanty vesicles, etc. 5. Considering the above findings, adrenalectomy probably causes the dysfunction of the gastric mucus cell that results in delayed mucus formation and secretion.

동시형광 분광광도법에 의한 대기 시료 중의 다환방향족 탄화수소(PAHs)의 분석

유광식,정선이,정지영 한국대기환경학회 2004 한국대기환경학회지 Vol.20 No.1

Determination of some PAHs in ambient air at Ulsan have been carried out by collection of the components into n-hexane followed by synchronous spectrofluorimetric technique. 10 PAHs, such as acenaphthene (Ace), anthracene (Anth), benz[a]anthracene (BaA), benzo[b]fluoranthene (BbFt), benzo[k]fluoranthene (BkFt) benzo [a]pyrene (BaP), chrysene (Chry), phenanthrene (Phen), fluoranthene (Ft), perlyrene (Per), and pyrene (Pyr) in air samples were able to determine separately by synchronous spectrofluorimetry. Calibration curves for those components were linear for the concentration range of 0.2∼166 ppb PAHs with the correlation factor of 0.9985∼0.9999. The predominant contribution was phenanthrene which was included 36.9∼85.1% to the overall level of the 10 PAHs in some areas, Also benzo[a]pyrene which was known to carcinogenicity was detected from 6.4 to 55.8 ng/㎥, benzo[a]anthracene of some areas was contained from 21.9∼153 ng/㎥,

중금속물질이 마우스 신장 근위곱슬세관의 미세구조에 미치는 영향

염광섭,박경호,양남길,안의태,고정식 순천향의학연구소 1995 Journal of Soonchunhyang Medical Science Vol.1 No.2

본 실험은 중금속 물질이 마우스 신장 근위요세관의 미세구조에 미치는 영향을 알아보기 시행하였다. 체중 20-25 g의 숫생쥐를 실험동물로 사용하였으며, 대조군과 중금속 투여군으로 나누었다. 중금속 투여군은 투여 량과 종류에 따라 염화제이수은(HgCl₂)투여군 (2mg, 5mg, 10mg/kg/BW)과 중크롬산칼륨(K₂Cr₂O7)투여군 (5mg, 10mg, 20mg/kg/BW)으로 나누어, 중금속 투여 후 6시간, 3일 및 2주 후에 도살하여 조직을 절취하였다. 중금속투여는 마우스의 등 쪽에 피하주사 하였다. 절취한 신장은 2.5% glutaraldehyde- 1.5% paraformaldehyde액으로 일차고정한 후, 1% osmium tetroxide액에 이차고정하였으며, 고정이 끝난 조직은 acohol과 acetone으로 탈수한 후 araldite 혼합액에 포매하였다. 포매된 조직은 LKB-V ultratome으로 60-70 nm 두께의 얇은 절편을 작성하여 uranyl acetate와 lead citrate액으로 염색한 후 JEM 100CX-Ⅱ전자 현미경으로 관찰하여 다음과 같은 결론을 얻었다. 1. 염화제이수은은 근위곧은세관에 먼저 영향을 주며, 중크롬산칼륨은 근위곱슬세관에 영향을 준다 2. 중금속이 신장의 근위요세관에 미치는 영향은 중금속의 투여량과 시간에 비례하는 변화를 보였다 3. 중금속은 세포의 막성구조들에 영향을 주어 기능을 저하시켜, 상피의 탈락을 일으켰으나, 시일이 경과하면 정상 상태로 복귀한다. 4. 근위요세관의 변화는 1) 미세융모는 수적 감소, 2) 다양한 크기의 세포질 내 액포형성, 3) 사립체의 변화 , 4) 무과립형질내세망의 증가, 5) 세포기저부와 옆면 세포사이간극의 확장 및 상피의 탈락, 6) 근위요세관의 상피재생의 순서로 일어난다. This experiment was performed to study the morphological changes of the proximal convoluted tubule of mice following subcutaneous injection of heavy metallic agents. Male mice were divided into normal and experimental groups. The mice were subcutaneous injected with HgCl₂(2mg, 5mg and 10mg/Kg/BW) and K₂Cr₂O7(5mg, 10mg and 20mg/kg/BW). Animals were sacrificed on 6 hours, 3 days and 14 days after the injections. Kidney were fixed in the 2.5% glutaraldehyde - 1.5% paraformaldehyde solution, followed by refixation in the 1% osmium tetroxide solution. Dehydrated blocks were embedded in araldite mixture. The sections were cut on a LKB V ultratome, and ultrathin sections stained with uranyl acetate and lead citrate were observed with JEM 100CX Ii electron microscope. The results were as follow: 1. Mercury chloride causes selective damage of straight portion of proximal tubule in the mouse kidney. 2. Potassium dichromate selectively affects the cells of the convoluted portion of the proximal tubule. 3. Tubular damage is greater at higher dose levels and at longer time intervals. 4. The sequence of progressive changes leading to nephron damage are : 1) swelling and loss of microvilli; 2) formation of vacuoles of varied sizes; 3) mitochondrial changes and accumulation of rough endoplsmic reticulum; 4) dilation of basolateral intercellular space followed by desquamation; and 5) regeneration of proximal tubular cell.

부신절제후의 위점막 점액세포의 미세구조

양남길,구광일,안의태,고정식,박경호 順天鄕大學 1989 순천향대학논문집 Vol.12 No.3

랫트의 생체내 카드뮴 단독투여 및 카드뮴과 셀레늄 병용투여에 따른 필수원소의 변화

김동현,변정식,조영채,이범식,김광환,김정윤 忠南大學校 環境問題硏究所 1995 環境硏究 Vol.13 No.-

This study was performed to find out the changes of essential elements as Cu, Fe, Zn, and Ca in blood, liver, kidney and testis of rats. The experimental rats were divided into 4 groups that were consisted of control group(groupⅠ), 3.0㎎Cd/kg group(groupⅡ), 3.0㎎Cd +1.0㎎Se/kg group(groupⅢ) and 3.0mgCd+2.5mgSe/kg group(groupⅣ). Each group was intraperitoneal injection with difference dose of cadmium and selenium such as 3.0mgCd/kg, 3.0mgCd+1.0mgSe/kg and 3.0mgCd+2.5mgSe/kg. Rats were killed at the time of 3 day and 28 day, and Cd, Se, Cu, Zn and Ca concentration in several organs were determined by Atomic Absorption Spectrophotometry and inductively Coupled Plasma. Cadmium content in blood, liver, kidney and testis were significantly increased in the Cd alone injection group than Cd mixed with selenium injection groups. But, copper content were not significantly differences in control and each experimental group. Iron content were significantly increased in the Cd alone injection group than others groups. Zinc and calcium content were significantly decreased in the Cd alone injection group than other groups. On the other hand the essential element content in Cd mixed with selenium injection group were almost equaled with those of control group than Cd alone injection group.