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Sang Jun Lee,Yun Hee Han,Young Ok Kim,Bo Hye Nam,Hee Jeong Kong,Kyung Kil Kim 한국분자세포생물학회 2010 Molecules and cells Vol.30 No.2
We examined whether the isoelectric point (pI) of the N-terminal region of the recombinant protein 7xMefp1 acts as a universal index for expression of the protein in solu-ble form in E. coli. Expression analysis of 7xMefp1 fused to various N-terminal sequences with pI values ranging from 2.73 to 13.35 yielded three pI range-specific curves (acidic, neutral, and alkaline curves at pI 2.73-3.25, 4.61-9.58, and 9.90-13.35, respectively) for soluble expression (by facilitated diffusion) as a proportion of total protein. For neutral N-termini (pI 4.61-9.58), the total amount of rMefp1 expressed was minimally affected by ∆GRNA for unfolding the mRNA secondary structure. The highly hy-drophilic nature of longer N-terminal sequences with strongly acidic and alkaline pI values reduced the transla-tion of rMefp1-encoding transcripts, thereby reducing the amount of soluble rMefp1 produced. After characterizing both feedback and non-feedback regulation in the acidic, alkaline, and neutral pI ranges, we suggest that three dif-ferent pI range-specific soluble expression curves exist for the recombinant protein, each defined by specific ranges of the leader sequence pI values.
Kong, Hee Jeong,Moon, Ji-Young,Nam, Bo-Hye,Kim, Young-Ok,Kim, Woo-Jin,Lee, Jeong-Ho,Kim, Kyung-Kil,Kim, Bong-Seok,Yeo, Sang-Yeob,Lee, Chang Hoon,Kang, Kwang-Il,Lee, Sang-Jun Elsevier 2011 Fish & shellfish immunology Vol.31 No.2
<P><B>Abstract</B></P><P>Autophagy is an important cellular response to starvation and stress, and plays critical roles in embryogenesis, development, cell death, cancer, and immunity. Beclin-1 is one of the central regulators of autophagy in mammals. In the present study, we isolated a PoBeclin-1 cDNA from the olive flounder (<I>Paralichthys olivaceus</I>) by screening a flounder gill cDNA library and rapid amplification of cDNA ends (RACE). The PoBeclin-1 cDNA we isolated encodes a 447-amino acid polypeptide containing a conserved Bcl-2-binding domain. The deduced amino acid sequence of PoBeclin-1 showed high degrees of sequence identity (80.5–95.3%) with Beclin-1 from human, frog, mouse, zebrafish, and pufferfish. PoBeclin-1 transcripts were detected from 1 day post-hatching and were found to be ubiquitously expressed in the healthy flounder. Expression of PoBeclin-1 mRNA was increased in the kidney and spleen of flounders challenged with viral hemorrhagic septicemia virus (VHSV). When infected with VHSV, PoBeclin-1-overexpressing HINAE cells had low level (about 26%) of VHSV G transcripts compared to control cells. Taken together, these results suggest that PoBeclin-1 may play a role in the innate immune response to viral infection in the flounder.</P> <P><B>Highlights</B></P><P>► PoBeclin-1 cDNA encodes a 447-aa polypeptide containing a conserved BH3 domain. ► Expression of PoBeclin-1 mRNA was increased in flounders challenged with VHSV. ► Overexpression of PoBeclin-1 decreased VHSV-G mRNA levels. ► PoBeclin-1 may play a role in the innate immunity to VHSV infection.</P>
Rim1α and Neurexin3 Regulate Synaptosomal Dopamine Release
Sang Kyeong Lee(이상경),Joo-Cheol Shim(심주철),Young-Hoon Kim(김영훈),Bo-Geum Kong(공보금),Dae-Hyun Seog(석대현) 한국생명과학회 2009 생명과학회지 Vol.19 No.9
신경전달물질의 분비는 시냅스전 신경말단의 active zone에 있는 다양한 단백질들에 의해 조절된다. 도파민은 정신분열병, 약물중독과 같은 여러 가지 행동, 정신질환의 병태생리와 연관된 필수적인 신경전달물질이다. 저자들은 본 연구에서 신경전달물질 분비와 관련된 주요 유전자가 결여된 knockout (KO) 생쥐의 시냅토좀(synaptosome) 도파민 분비를 측정하였다. 시냅토좀 도파민 흡수와 분비는 [³H]-도파민과 관류실험을 이용하여 시행, 측정하였다. 17 KO 생쥐 가운데 3 종류의 생쥐에서 그들의 littermate 대조군과 비교하였을 때 변화된 도파민 분비를 보였다. Rim1α KO에서 세포막 탈분극에 의한 [³H]-도파민은 유의하게 감소되었으며, 또한 Rim1α의 도파민 신경에서의 조건 KO에서는 생리적 완충용액에 의한 기본적인 도파민 분비 및 세포막 탈분극에 의한 도파민 분비 모두가 유의하게 감소되어 있었다. neurexin3의 도파민 신경에서의 조건 KO에서는 세포막 탈분극에 의한 도파민 분비의 증가를 보였다. 이 데이터들은 도파민 분비와 글루타메이트, GABA와 같은 전통적 신경전달물질 분비의 유사성과 차별성을 설명한다. 결론적으로, Rim1α와 neurexin3는 시냅스전 도파민 분비의 중요한 조절자이며 신경계 질환과 연관될 가능성이 있다. Neurotransmitter release is regulated by various proteins of the active zone in the presynaptic nerve terminals. Dopamine (DA) is an essential neurotransmitter associated with the pathophysiology of diverse behavioral and mental illness such as schizophrenia and drug addiction. We measured synaptosomal DA release of knockout (KO) mice which lacked major genes related to neurotransmitter release. Synaptosomal DA uptake and release were performed and measured using [³H]-DA and superfusion experiments. 3 of the 17 KO mice exhibited altered DA release compared to their littermate controls. In Rim1α KO, [³H]-DA release evoked by membrane depolarization significantly decreased. Both basal (physiological buffer-evoked) and membrane depolarization-evoked DA release significantly decreased in dopaminergic conditional KO of Rim1αβ. Dopaminergic conditional KO of neurexin3 demonstrated a significant increase of membrane depolarization-evoked DA release. These data explain the similarities and distinctions between DA and other classical neurotransmitters such as glutamate and GABA (γ-aminobutyric acid) release. In conclusion, Rim1α and neurexin3 may be important regulators of presynaptic DA release and related to disorders of the nervous system.
Influence of underlying layers on the growth of 1D (Mg,Zn)O nanorods
Kong, Bo Hyun,Jun, Sang Ouk,Kim, Young Yi,Kim, Dong Chan,Cho, Hyung Koun WILEY-VCH Verlag 2007 Physica Status Solidi. B Vol.244 No.5
<P>We grew (Mg,Zn)O nanorods with different morphologies and growth directions on various substrates by the thermal evaporation process using vapor-solid growth mode. The alignment and growth mode of the nanorods are significantly affected by the underlayer and substrates. By depositing ZnO and (Mg,Zn)O underlayers, the alignment of the (Mg,Zn)O nanorods was changed from random to vertical based on the substrate surface. (© 2007 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim)</P>
Kong, Min-kyu,Park, Tusan,Shim, Sung-Bo,Jang, Ik Joo Korean Society for Agricultural Machinery 2017 바이오시스템공학 Vol.42 No.4
Purpose: It is to develop an agricultural three-directional dumping vehicle that can help farmers reduce intensive labor when carrying heavy loads and for easy dumping. In addition, a novel mechanism was applied for controlling the direction of the tilting cargo box by using a single hydraulic cylinder and simple apparatus. The overturning safety was analyzed to provide safe-use ground slope region of the vehicle to be used at upland fields and orchards. Methods: The developed three-directional dumping vehicle was constructed using a cargo box, vehicle frame, driving components, lifting components, and controller. The novel mechanism of controlling the dumping direction involves the operation of two latching bars, which selectively release or collapse the connecting edge between the vehicle frame and cargo box. A multibody dynamics analysis software (RecurDynV8R5) was used to determine the safe-use ground slope area when tilting the cargo box at slopes. A computer analysis was conducted by increasing the ground slope while rotating the vehicle when the cargo box comprised loads of 300 and 500 kg and stacking heights of 40 and 80 cm, respectively. Results: The three-directional dumping vehicle was successfully manufactured, and the cargo box was tilted at $37^{\circ}$ and $35^{\circ}$ for dumping forward and sideways. The latching bars were manually and selectively collapsed with the vehicle frame to control the dumping direction. When forward dumping, the safe-use ground slope was over $20^{\circ}$ in all vehicle directions and loaded conditions. Conclusions: A three-directional dumping vehicle was developed to reduce labor-intensive work in the farming environment. The user can easily control the dumping direction by using the control panel. The vehicle was safe to be used in most of the Korean upland fields and orchards (area over 96%) for the forward dumping.
알코올 의존 환자에서의 12단계 척도의 신뢰도와 타당도 연구
정성수(Sung Soo Jung),하태민(Tae Min Ha),김경승(Kyung Seung Kim),이성근(Seong Keun Lee),심주철(Joo Cheol Shim),공보금(Bo Geum Kong),정도운(Do Un Jung) 대한생물치료정신의학회 2014 생물치료정신의학 Vol.20 No.1
Objectives:This study is aimed to assess the reliability and validity of the Twelve Step Scale which are newly developed to observe the direct effect of the twelve step facilitation therapy that has been widely used for the treatment of patients with alcohol dependence. Methods:110 patients who were diagnosed with alcohol dependence by psychiatrists using DSM-TR criteria agreed to participate in the study. Subjects were assessed by questionnaires including demographic data and alcohol related clinical rating scales such as Twelve Step Scale, Korean version of Alcohol Use Disorders Identification Test, Alcohol Dependence Scale, Hanil Alcohol Insight Scale when informed consents were acquired. Four weeks later after initial assessment, Twelve Step Scale was reassessed for subjects who were treated over 3 months at the same hospital without recent admission or discharge for a month. Results:The average of the Twelve Step Scale was higher in female patients than in male. In addition, there is positive correlation between HAIS and Twelve Step Scale. According to factor analysis, Twelve Step Scale has two factors. The results showed significant internal consistency reliability(Cronbach’s α=0.92) but did not show reasonable test-retest reliability. Conclusion:This study showed that the Twelve Step Scale had reasonable reliability and validity except test-retest reliability. Thus the Twelve Step Scale can be used to assess the direct effects of twelve step facilitation therapy, which in turn might contribute to enhance twelve step facilitation therapy in the future.
김성진(Sung-Jin Kim),심주철(Joo-Cheol Shim),공보금(Bo-Geum Kong),강제욱(Je-Wook Kang),문정준(Jung-Joon Moon),임지섭(Ji-Seop Lim),김임규(Im-Gyu Kim),김연수(Yeon-Sue Kim),오민경(Min-Kyung Oh),정도운(Do-Un Jung) 대한생물치료정신의학회 2013 생물치료정신의학 Vol.19 No.2
Objectives:Cognitive dysfunction is one of the major symptoms of schizophrenia, and has a diverse effect on the prognosis and course of schizophrenia. Several studies have been done about the correlation between cognitive dysfunction and clinical symptoms of schizophrenia, but the results of those studies were not clear. This study was performed to confirm the correlation between cognitive dysfunction and clinical symptoms of schizophrenia. Methods:We studied 117 patients with schizophrenia. We assessed clinical symptoms and the cognitive function of the patients, and analyzed the statistical correlation between clinical symptoms and the cognitive function. For clinical symptoms, Positive and Negative Syndrome Scale(PANSS), Clinical Global Impression-Severity(CGI-S) and Hamilton Depression Rating Scale(HDRS) were performed. For cognitive function, Digit Span Test, Visual Span Test, Stroop Color Word Test(SCWT), Continuous Performance Test(CPT), Wisconsin Card Sorting Test(WCST), and Digit Symbol Substitution Test(DSST) were performed. Results:There was significant correlation between CPT and clinical symptoms. The WCST revealed that Categories Achieved(CA) have significant correlation with disorganization symptoms among 5-factor model of PANSS (p<0.05). DSST revealed that the total score(p<0.01), negative syndrome(p<0.01), general psychopathology(p<0.01) among 3 kind major area classification of PANSS and disorganization symptoms of 5-factor model of PANSS(p<0.001) have significant correlation. Conclusion:This study revealed the correlation between the cognitive function and a part of clinical symptoms in patients with schizophrenia, as shown through the CPT, WCST and DSST.