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        The aquatic macrophyte flora of a small pond revealing high species richness in the Aomori Prefecture, Japan

        Kohtaroh Shutoh,Takashi Yamanouchi,Syou Kato,Hiroki Yamagishi,Yusuke Ueno,Shiori Hiramatsu,Jun Nishihiro,Takashi shiga 국립중앙과학관 2019 Journal of Asia-Pacific Biodiversity Vol.12 No.3

        A small semiartificial pond, revealing high species richness with respect to the aquatic macrophytes, was studied in the Aomori Prefecture, Japan. The pond had been completely formed ca. 10 years previously after excavation into a wet bog. To identify and evaluate its aquatic macrophyte flora during 2017–2018, we listed the species of aquatic vascular plants and charophytes in the pond and compared this list with those from the natural Japanese lakes using a database of aquatic flora in Japan. Two species were identified by molecular analyses because they lacked any reproductive organs, which were necessary for identifying the species level. We found a total of 57 taxa of aquatic macrophytes including 15 Red List species in Japan or Aomori Prefecture. Comparing the flora list with those from the 66 natural Japanese lakes surveyed since 2001, the pond was ranked fourth in the order of diversity (based on species richness) and rarity (based on numbers of Red List species). Therefore, the pond is an important aquatic environment in terms of aquatic macrophyte conservation in Japan. Interestingly, for a pond with such high species diversity, it is relatively small (0.14 km2), semiartificial, and relatively recently formed.

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        Obtaining new records of critically endangered Potamogeton praelongus (Potamogetonaceae) depending on groundwater springs in northern coastal areas of Hokkaido, Japan

        Kohtaroh Shutoh,Tomoki Hirose,Takashi Shibahara,Takashi Shibahara,Mami Yamazaki,Takashi shiga 국립중앙과학관 2022 Journal of Asia-Pacific Biodiversity Vol.15 No.4

        Potamogeton praelongus Wulfen is an aquatic macrophyte that is critically endangered in Japan. Althoughit is widely distributed throughout the boreal climatic zone, several European countries also recognize itas an endangered species. In Japan, it was once widely distributed throughout Hokkaido but has dis appeared or reduced in abundance at its known locations. During 2020e2021, we discovered five newlocations of this species in Wakkanai-shi and Sarufutsu-mura, Northern Hokkaido. Growing conditionsmust be suitable for this species in three of the five locations as it is present with several reproductiveshoots or is widely distributed in source pond or lake. However, conditions were poor in the other twolocations because few shoots were found or plants were observed only in 2020. Environmental condi tions in the new locations were similar to those reported in Europe and North America. In three of thefour locations at Wakkanai-shi, P. praelongus was distributed mainly along the eastern shore facing sanddunes or hills, suggesting that the distribution of this species depends on groundwater springs flowingfrom sand dunes or hills. Further populations may be discovered in the future by intensively surveyingareas near springs since many sand dune lakes and similar environments are present in Hokkaido.

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        Molecular phylogenetic relationships among populations of Sagittaria aginashi Makino (Alismataceae) and endemic Chinese species

        Kazumasa Tsubota,Kohtaroh Shutoh,Syou Kato,최혁재,Takashi shiga 국립중앙과학관 2019 Journal of Asia-Pacific Biodiversity Vol.12 No.1

        The aquatic plant Sagittaria aginashi, which is an endangered emergent species distributed in SouthKorea, Japan, and the Russian Far East, has an unclear phylogeny and genetic structure. Phylogenetic treesof Sagittaria were constructed based on one nuclear ribosomal DNA (nrDNA) region and on threechloroplast DNA (cpDNA) regions. S. aginashi was included in a clade comprising three endemic ChineseSagitarria species characterized by the presence of small tubercles at the base of the axils. Furthermore,phylogenetic trees based on cpDNA sequences indicated that S. aginashi was more closely related toS. tengtsungensis than to two other species. S. aginashi exhibited little genetic variation, suggesting thatthis species had rapidly spread in these areas. With regard to factors associated with the rapid spread, weconsidered that multiple dispersal methods were involved, namely hydrochory via tubercles, endozoochorous long-distance dispersal, and anthropogenic impacts of rice farming on S. aginashi dispersal.

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