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Preparation of Polymer-Coated Magnetite Fine Particles for Immunoassay Magnetic Marker
문정민,김봉수,최연실,이장우,Tae Nakahara,Kohji Yoshinaga 한국고분자학회 2010 Macromolecular Research Vol.18 No.8
Polymer-coated magnetite fine particles with carboxyl groups were developed for applications as an immunoassay magnetic marker. The magnetite composites were prepared using the following method. A hydrophilic macromer with a polymerizable vinyl group at the terminal was adsorbed on the surface of the magnetite fine particles. Subsequently, a hydrophilic vinyl monomer with a carboxyl group and a crosslinking agent were added to carry out a copolymerization reaction. The resulting magnetite composite particles were dispersed stably in an aqueous medium. The composite particles exhibited high antibody binding ability and high-sensitive magnetic properties suitable for applications as magnetic markers.
Moon, Jung-Min,Kim, Bong-Soo,Paik, Hyun-Jong,Lee, Jang-Oo,Mouri, Emiko,Yoshinaga, Kohji Wiley Subscription Services, Inc., A Wiley Company 2010 Polymer engineering and science Vol.50 No.6
<P>Silica nanoparticles with well-defined, highly grafted dense poly(methyl methacrylate) (MMA) were prepared by surface-initiated activators regenerated by electron transfer for atom transfer radical polymerization (ARGET ATRP) of methyl methacrylate with an initiator-fixed silica particle in the presence of air. Two different polymerizations of MMA were carried out under the same conditions using tris[2-(dimethylamino)ethyl]amine (Me<SUB>6</SUB>TREN) and N,N,N′,N′,N″-pentamethyldiethylene-triamine (PMDETA) as the ligand, respectively. In the CuCl<SUB>2</SUB>/PMDETA system, polymerization appeared to be more controlled with a lower polydisperisty compared with the CuCl<SUB>2</SUB>/Me<SUB>6</SUB>TREN system. The monolayer of these particles was formed at the air–water interface using Langmuir-Blodgett (LB) technique. Multilayers of the particles were fabricated by repetition of LB depositing. A surface pressure–area (π–A) measurement and SEM observation were used to characterize the particle arrays. POLYM. ENG. SCI., 2010. © 2009 Society of Plastics Engineers</P>