Development of a noninvasive KIM-1-based live-imaging technique in the context of a drug-induced kidney-injury mouse model

Tae-Jun Kwon,Da-Sol Lee,Md. Enamul Haque,Rang-Woon Park,Byungheon Lee,Dongkyu Kim,Yong-Hyun Jeon,Kil-Soo Kim,Sang Kyoon Kim 한국실험동물학회 2021 한국실험동물학회 학술발표대회 논문집 Vol.2021 No.7

The development of reliable methods to diagnose acute kidney injury is essential to allow the adoption of early therapeutic interventions and evaluate their effectiveness. Based on the fact that kidney injury molecule-1 (KIM-1) expression levels in kidneys are markedly upregulated early after a damage event, here we developed a noninvasive KIM- 1-based molecular imaging technique to detect kidney injury. First, we took advantage of a phage-display platform to select small peptides demonstrating a specific high binding affinity to KIM-1. The promising candidate was conjugated with fluorescent probes, and its imaging potential was validated in vitro and in vivo. This peptide, with the sequence CNRRRA, not only showed a high imaging potential in vitro, allowing a strong detection of KIM-1 expressing cells by microscopy and flow cytometry but also generated a strong kidney-specific signal in live-imaging in vivo experiments in the context of a drug-induced kidney-injury mouse model. Our data overall suggest that the CNRRRA peptide is a promising probe to use in the context of in vivo imaging for the detection of KIM-1 overexpression in damaged kidneys.

C 의과대학에서 문제강의에 대한 학생들의 직접평가가 강의개선에 미친 영향

김석용, 이상진, 최재운, 김승렬, 김용대, 배성은, 김영규 충북대학교 의과대학 충북대학교 의학연구소 2012 忠北醫大學術誌 Vol.22 No.1

연구목적: 팀티칭에 근거한 통합강의는 최근 대부분의 의과대학에서 시행되는 강의형태이다. 그러나 기 존에 사용되고 있는 온라인 강의평가 방식은 이러한 강의 특성에서 나타나는 문제점을 파악하기 힘든 점이 있다. 본 대학에서는 팀티칭에 의한 통합강의에서 문제강의를 직접적으로 파악하는 ‘문제강의 직 접평가지’를 개발하였다. 본 연구의 목적은 새로이 개발된 문제강의 직접평가지가 강의개선에 끼친 영 향력을 알아보고자 하는 것이었다. 연구방법: 2007년 2학기부터 2009년 2학기까지 충북대학교 의과대학 1, 2학년을 대상으로 문제강의 직접평가지에 의한 강의평가를 실시하였다. 평가지 질문 중에서 두 가지 객관식 질문에 대한 답변을 분 석대상으로 선택하였다. 질문은 첫째, ‘가장 유익하고 도움이 많이 되었던 강의들은 무엇입니까?’와 둘 째, ‘가장 어렵거나 이해하기 힘들었던 강의들은 무엇입니까?’였다. 강의평가를 실시한 강좌들 중에서 2 년 이상 연속적으로 강좌구조와 평가지의 객관식 질문 형식이 변하지 않은 14개의 강좌를 분석대상으로 하였다. 각각 연도의 평균 긍정비율을 다른 연도의 것과 카이제곱 검정 방법을 사용하여 비교하였다. 결과: 14개의 강좌 중에서 시작연도와 마침 연도 간에 긍정비율이 증가된 경우는 13개 강좌였다. 그 러나 통계적으로 의미가 있는 경우는 5개 강좌에서 나타났다. 결론: 문제강의 직접평가지에 의한 문제강의를 판별해 내는 방법은 단기간에 통합강좌의 개선에 효과 적이다.

부산시 용호동 정책이주단지 주거공간의 내·외부 변용 특성에 관한 연구

김용운(Kim, Yong-Woon),유재우(You, Jae-Woo) 대한건축학회 2014 대한건축학회 학술발표대회 논문집 Vol.34 No.2

Accuracy on the Edge Detection of Topography for Digital Map Manufacture

Park, Woon-Yong,Song, Youn-Kyung,Bae, Kyung-Ho,Kim, Yong-Suk 東亞大學校 建設技術硏究所 2005 硏究論文集 Vol.28 No.2

Currently, digital maps in the metropolitan area are supposed to be revised and renewed every two years, but it is difficulty for the renewal of current information to keep pace with the quickly changing society and so, one of the alternative methods being discussed is a digital map produced using high resolution satellites. The biggest problem in the production of digital maps using high resolution satellite images is the difficulty of production in vector resource format through edge detection of satellite images, a Raster data. In this study, topographical boundaries were detected through edge detection of the satellite image, and after vectorliezing using the screen digitizing method, the results were evaluated and compared to the digital map from the NGII(National Geographic Information Institute) for accuracy.

강변여과에서 파일럿규모 수평집수관의 수두분포 연구

정재민(Jae Min Jeong),박재영(Jae Young Park),이종진(Jong Jin Lee),김용운(Yong Woon Kim),김승현(Seung Hyun Kim) 大韓環境工學會 2013 대한환경공학회지 Vol.35 No.5

강변여과용 수평집수관을 모사하는 파일럿 규모의 모래통 실험을 수행하였으며, 다양한 출구유속에 대한 수평집수관에서의 수두분포를 구하였다. Kim24)이 수행한 실험실 규모의 수평집수관에서의 손실수두에 Kim25)이 개발한 이론 즉, 작은 규모의 수평집수관에서의 손실수두로부터 큰 집수관의 손실수두를 계산하는 방법을 적용하여 본 연구의 관측치와 비교함으로써 Kim25)의 이론이 타당함을 알 수 있었다. 또한, 이들의 이론을 적용하여 본 연구에서의 관측치로부터 실규모 집수관에서의 수두분포를 구하는 방안을 예를 들어 설명함으로써 강변여과의 수평집수관 설계방법을 제시하였다. A pilot-scale sand-box experiment simulating a lateral of collector well used in riverbank filtration was performed, and hydraulic head distributions along the lateral were measured according to the various drawdowns at the well. The results of this experiment were compared to the predicted values obtained using a theory previously developed by Kim25) in order for its validation. This theory predicts the head loss in a large-scale lateral given the loss in a small-scale one, and to have actual values for comparison, the results from a lab-scale experiment previously performed by Kim24) were employed. Comparing the experimental values to the extrapolated value indeed confirmed the validity of the theory. A procedure to determine the hydraulic head distribution of a practical-scale lateral was also presented applying the theory to the experimental result of this study in an effort to show the process of lateral design for riverbank filtration.

電解法에 依한 Polyvinyl Alcohol 含有 廢水處理에 關한 硏究

김운수,차시환,김용욱 慶熙大學校 材料科學技術硏究所 1991 材料科學技術硏究論集 Vol.4 No.-

The purpose of this study is to investigate into the electrolytic treatment of polyvinyl alcohol wastewater. In order to remove PVA from wastewater by electrolytic treatment, Fe electrode was used as a soluble electrode for this study. The results of this sutdy are as follows: 1. PVA was removed nearly to 100% as the value of ampere hour concentration was more than 0.5 Ahr/ℓ. 2. PVA was removed quickly as the current density was rised, the electrode distance was approached, NaCl was added and the initial pH of wastewater was above 7. 3. Regardless of the initial pH of wastewater, the pH of wastewater after electorolytic treatment was among 10 and 11. So, it is necessary to neutralize the treated water.

12주 운동프로그램의 참여가 남녀 노인의 혈중지질 및 성장호르몬과 면역반응에 미치는 영향

김상우,배윤정,이운용 한국특수체육학회 2002 한국특수체육학회지 Vol.10 No.2

The purpose of this study was to examine the changes of blood lipid profile, growth hormone, and immune responses in elderly population after a 12 week exercise program. A total of 11 elderly aged between 65-70yrs participated for this study. results as follows : In men, barbell lifting, grip strength, sit & reach, VO_2max were significantly increased(p<.05). For elderly women, lower back strength, grip strength, sit & reach, and VO_2max were significantly elevated(p<.05). The total cholesterol and triglycerides were significantly decreased in both sexes(p<.05). While no differences were found in growth hormone in both sexes, estrogen was significantly increased in both sexes(p<.05). Epinephrine was decreased in both sexes(p<.05), but norepinephrine showed only a tendency of increment. Only NK cell but not T cell and B cell was statistically significant in elderly women(p<.05). The 12 week exercise program had influenced on muscular strength, cardiopulmonary function, sex hormones, and catecholamine levels in elderly population.

높이뛰기競技의 評價基準에 關한 硏究

金永煥,崔龍雲,朴鉉權 慶北大學校 師範大學 體育學硏究會 1990 體育學會誌 Vol.18 No.-

We gained the results like next, according to the analysis and measurement of records on High Jump, in male 467, female 74, from 1980 to 1989(for 10 years). (1) We gained the distribution chart like (figure 3) (figure 4) and also We gained the average Value like (figure 5). (2) We gained a new Standard Value of evaluation High Jump like(figure 9). (3) As we know in(figure 10) In the result of Comparison between old and new standard Values, We can gain following, a) In the cases of male, it is increase by average 2㎝. b) In the cases of female, the Evaluational of student gained record 115㎝, old Standard is Score of 80, but new Standard is decrease of score 5 by score 75. From 1991 One's junior Season, We decided to apply this new Standard Value.

폐수처리 공정 중 휘발성 유기화합물의 분석에 관한 연구

김영두,양용운,김재현 啓明專門大學 産業開發硏究所 2001 啓明硏究論叢 Vol.19 No.1

D염색단지 폐수처리장 유입원수 및 집수조, 1차 화학처리수, 2차 생물처리수 및 최종방류수의 VOC를 분석한 결과 dichloromethane, chloroform, toluene 등이 검출되었으며, 유입원수에서는 각각 45.8㎍/ℓ, 40.8㎍/ℓ, 24.5㎍/ℓ가 검출되었다. VOC성분은 대부분 집수조 및 cooling tower공정에서 휘발에 의해 제거되었고. 최종방류수의 dichloromethane, chloroform, toluene은 0.37㎍/ℓ, 0.29㎍/ℓ, 0.46㎍/ℓ를 나타내었다. 그리고 원수에 함유된 VOC 농도는 여름철이 겨울철에 비해 높았고, 제거효율은 여름철이 더 우수하였다. This study was conducted to investigate VOCs concentration of influent wastewater, storage tank, 1st chemical treatment effluent, 2nd biological treatment effluent and final effluent in D dyeing complex wastewater treatment plant for one year from July 1999 to June 2000. As a result, the small amount of VOCs such as dichloromethane, chloroform, toluene were detected and concentration of dichloromethane, chloroform, toluene in influent wastewater were 45.8㎍/ℓ, 40.8㎍/ℓ and 24.5㎍/ℓ respectively. The VOCs such as dichloromethane, chloroform, toluene were removed by volatilization in storage tank and cooling tower process. After wastewater treatment process, concentration of dichloromethane, chloroform, toluene in final effluent were 0.37㎍/ℓ, 0.29㎍/ℓ and 0.46㎍/ℓ, respectively. In addition, seasonal variation of VOCs concentration in influent wastewater showed higher in the summer than winter, but removal efficiencies were excellent in the summer.

박스-젠킨스법을 이용한 소양호 수질변화 시계열분석

김범철,윤용운,정태영,박석순 이화여자대학교 환경문제연구소 1997 이화환경연구 Vol.1 No.-

Statistical models were developed using the Box-Jenkins time series analysis to simulate changes of water qualities in Soyang Lake. The water qualities included in the models are total phosphorus(TP) and chlorophyll-a(CA). The model development was based on the data obtained from Feb. 1987 to Oct. 1992 for TP and from Mar. 1985 to Oct. 1992 for CA, and followed the typical procedures of the Box-Jenkins method including identification, estimation, and diagnostic check. From the computed autocorrelation function (ACF) and partial autocorrelation function(PACF), it was determined that the TP model should have two autoregression (AR) parameters and one moving average(MA) parameter, and two seasonal AR parameters and one seasonal MA parameter. The CA model was determined to have two AR parameters and two MA parameters, and two seasonal AR parameters and on seasonal MA parameter. Both models had seasonality of twelve months and were formulates as SARIMA(2,1,1)×(2,1,1)₁₂for TP and SARIMA(2,0,2)×(2,1,1)₁₂ for CA. The models were validated by testing normality and independency of the residuals. The model prediction ability was tested using the data collected from Nov. 1992 to July 1994 both for TP and CA. There were good agreements between the model predictions and the field measurements. Most of the predicted values were located within the 95% confidence limits except certain period of chlorophyll-a. It was concluded from this study that the Box-Jenkins time series analysis would be an effective tool to understand and simulate the long term variations of water qualities in Soyand Lake.