http://chineseinput.net/에서 pinyin(병음)방식으로 중국어를 변환할 수 있습니다.
변환된 중국어를 복사하여 사용하시면 됩니다.
Sugar Alcohol, Erythritol has Antioxidant Properties on Miniature Pig Sperm Cryopreservation
Seunghee Kim,Haein Kim,Gahee Kim,Wooyoung Park,Daeyoung Jeong,Wonjae Kim,Daeyoung Kim 한국수정란이식학회 2016 한국수정란이식학회 학술대회 Vol.2016 No.10
Cryopreservation of miniature pig sperm is essential because of high demand of organ transplant in mass production. However, miniature pig sperm are vulnerable to oxidative stress more than other mammals. Erythritol is a naturally occurring sugar alcohol with powerful antioxidant property. Thus, the aim of our study is to verify if erythritol could reduce lipid peroxide and enhance viability of frozen thawed miniature pig sperm. Ejaculated semen samples were frozen with cryoprotectant subjected to erythritol treatment (0, 10, 100, 500 mM). After frozen thawed, spematozoa viability were examined using the computer assisted sperm analysis (CASA) system. The product of lipid peroxidation, malondialdehyde (MDA) were quantified using spectrophotometer with DPPH and ABTS assays as ROS scavenger markers. Our result showed that erythritol enhanced sperm viability (p<0.05), reduced lipid peroxides significantly (p<0.05), proving the concentration of 100 mM erythritol to be an effective for lowing oxidative damage. Data from our study suggest that erythritol exhibits significant lipid peroxidation scavenging characteristics which may prevent oxidative damage, enhance viability of frozen thawed sperm and thus could be a effective additive as cryoprotectant.
Kim, Haein,Yoon, Jae Bok,Lee, Jundae Korean Society of Horticultural Science 2017 원예과학기술지 Vol.35 No.4
Chili pepper (Capsicum annuum L.) is an economically important horticultural crop in Korea; however, various diseases, including Phytophthora root rot, anthracnose, powdery mildew, Cucumber mosaic virus (CMV), Pepper mild mottle virus (PMMoV), and Pepper mottle virus (PepMoV), severely affect their productivity and quality. Therefore, pepper varieties with resistance to multiple diseases are highly desired. In this study, we developed 20 SNP type assays for three pepper populations using Fluidigm nanofluidic dynamic arrays. A total of 4,608 data points can be produced with a 192.24 dynamic array consisting of 192 samples and 24 SNP markers. The assays were converted from previously developed sequence-tagged-site (STS) markers and included markers for resistance to Phytophthora root rot (M3-2 and M3-3), anthracnose (CcR9, CA09g12180, CA09g19170, CA12g17210, and CA12g19240), powdery mildew (Ltr4.1-40344, Ltr4.2-56301, and Ltr4.2-585119), bacterial spot (Bs2), CMV (Cmr1-2), PMMoV (L4), and PepMoV (pvr1 and pvr2-123457), as well as for capsaicinoids content (qcap3.1-40134, qcap6.1-299931, qcap6.1-589160, qdhc2.1-1335057, and qdhc2.2-43829). In addition, 11 assays were validated through a comparison with the corresponding data of the STS markers. Furthermore, we successfully applied the assays to commercial $F_1$ cultivars and to our breeding lines. These 20 SNP type assays will be very useful for developing new superior pepper varieties with resistance to multiple diseases and a higher content of capsaicinoids for increased pungency.
Haein Kim,Jae Bok Yoon,Jundae Lee 한국원예학회 2017 원예과학기술지 Vol.35 No.4
Chili pepper (Capsicum annuum L.) is an economically important horticultural crop in Korea; however, various diseases, including Phytophthora root rot, anthracnose, powdery mildew, Cucumber mosaic virus (CMV), Pepper mild mottle virus (PMMoV), and Pepper mottle virus (PepMoV), severely affect their productivity and quality. Therefore, pepper varieties with resistance to multiple diseases are highly desired. In this study, we developed 20 SNP type assays for three pepper populations using Fluidigm nanofluidic dynamic arrays. A total of 4,608 data points can be produced with a 192.24 dynamic array consisting of 192 samples and 24 SNP markers. The assays were converted from previously developed sequence-tagged-site (STS) markers and included markers for resistance to Phytophthora root rot (M3-2 and M3-3), anthracnose (CcR9, CA09g12180, CA09g19170, CA12g17210, and CA12g19240), powdery mildew (Ltr4.1-40344, Ltr4.2-56301, and Ltr4.2-585119), bacterial spot (Bs2), CMV (Cmr1-2), PMMoV (L4), and PepMoV (pvr1 and pvr2-123457), as well as for capsaicinoids content (qcap3.1-40134, qcap6.1-299931, qcap6.1-589160, qdhc2.1-1335057, and qdhc2.2-43829). In addition, 11 assays were validated through a comparison with the corresponding data of the STS markers. Furthermore, we successfully applied the assays to commercial F1 cultivars and to our breeding lines. These 20 SNP type assays will be very useful for developing new superior pepper varieties with resistance to multiple diseases and a higher content of capsaicinoids for increased pungency.
The Expanding Significance of Inositol Polyphosphate Multikinase as a Signaling Hub
Kim, Eunha,Ahn, Hyoungjoon,Kim, Min Gyu,Lee, Haein,Kim, Seyun Korean Society for Molecular and Cellular Biology 2017 Molecules and cells Vol.40 No.5
The inositol polyphosphates are a group of multifunctional signaling metabolites whose synthesis is catalyzed by a family of inositol kinases that are evolutionarily conserved from yeast to humans. Inositol polyphosphate multikinase (IPMK) was first identified as a subunit of the arginine-responsive transcription complex in budding yeast. In addition to its role in the production of inositol tetrakis- and pentakisphosphates ($IP_4$ and $IP_5$), IPMK also exhibits phosphatidylinositol 3-kinase (PI3-kinase) activity. Through its PI3-kinase activity, IPMK activates Akt/PKB and its downstream signaling pathways. IPMK also regulates several protein targets non-catalytically via protein-protein interactions. These non-catalytic targets include cytosolic signaling factors and transcription factors in the nucleus. In this review, we highlight the many known functions of mammalian IPMK in controlling cellular signaling networks and discuss future challenges related to clarifying the unknown roles IPMK plays in physiology and disease.
Magnetic properties of large-scaled MnBi bulk magnets
Kim, Sumin,Moon, Hongjae,Jung, Hwaebong,Kim, Su-Min,Lee, Hyun-Sook,Choi-Yim, Haein,Lee, Wooyoung Elsevier 2017 Journal of alloys and compounds Vol.708 No.-
<P><B>Abstract</B></P> <P>We investigated the magnetic properties of large, compacted, sintered MnBi bulk magnets with dimensions of 20.3 × 15.3 × 10.3 mm<SUP>3</SUP>. To obtain a high content of the low-temperature-phase (LTP) of MnBi in the precursor powders, a new process was implemented and produced about 98 wt% of LTP. To improve the coercive field of MnBi, particle sizes were controlled using different milling techniques. The dependence of magnetic properties of the bulk magnets on the particle size was analyzed. The highest maximum energy product, (<I>BH</I>)<SUB>max</SUB>, obtained among our samples was 7.3 MGOe. This is the first report of demonstrating high performance in large-sized MnBi bulk magnets.</P> <P><B>Highlights</B></P> <P> <UL> <LI> MnBi has a great potential as a rare-earth-free permanent magnet that can be used in high temperature (∼200 °C) applications. </LI> <LI> High performance in large-sized MnBi bulk magnets is reported for the first time. </LI> <LI> High-quality precursor powders were prepared by combining new procedures of cold-pressing and magnetic separation. </LI> <LI> Magnetic properties of bulk magnets were controlled using different milling techniques. </LI> </UL> </P> <P><B>Graphical abstract</B></P> <P>[DISPLAY OMISSION]</P>
Effect of Silicon Additions on the Magnetic Properties for Fe-Based Alloys
Kim, Sumin,Lee, Seung-Yub,Kim, Young Keun,Choi-Yim, Haein American Scientific Publishers 2016 Journal of Nanoscience and Nanotechnology Vol.16 No.11
<P>The effect of the Si additions on the magnetic properties is reported for the novel Fe87-xTi7Zr6Six (x = 0, 2, 4, 6, 8, 10) alloy system. The 2 mm wide ribbon samples for each composition were prepared by the melt spinning technique, and their phase information and magnetic behavior were obtained from X-ray diffraction and a vibrating sample magnetometer (VSM) respectively. The Fe87Ti7Zr6 system, reference alloy without Si (x = 0), is fully amorphous and has both paramagnetic and ferromagnetic properties; all other alloys with Si additions (x = 2, 4, 6, 8, 10) form crystalline phases, mainly alpha-Fe, which causes a magnetic phase transition from paramagnetic to ferromagnetic materials. The saturation magnetization (M-s) increases with increasing amount of Si addition, up to 1.16 T at x = 10. Temperature dependent dynamic magnetization is also observed in the Fe79Ti7Zr6Si8 (x = 8) system. Since Fe79Ti7Zr6Si8 alloy contains both a crystalline and amorphous phase, magnetization increases above the measured Curie temperature (T-c = 604 K) as a result of a higher crystallization temperature (T-x) of Fe-based amorphous alloy.</P>