人 諸臟器 Alkaline Phosphatase Isoenzyme의 電氣泳動 및 定量

金在植,金京善,金重明,金榮泰,蔣卓仙,李鉉基 慶北大學校 醫科大學 1980 慶北醫大誌 Vol.21 No.2

著者들은 家兎와 人體로부터 採取한 血淸과 諸臟器組織을 使用하여 ALPI 電氣泳動像을 觀察하고 生化學的 定量法을 利用하여 ALP의 活性度를 測定試圖하였으며 臟器特異性 沮害因子에 對한 影響을 觀察하였다. 家兎 血淸 및 諸臟器組織 ALPI電氣泳動에서 血淸은 ±의 活性度를 가진 α2와 α2/β分劃을 나타내었으며 肝組纖은 血淸보다 빠른 移動度를 가진 ??의 α_1과 α_2分劃을 나타내었다. 그리고 肝, 骨, 腎 淋巴腺, 脾臟 및 腸組織에서는 顯著하게 높은 活性度를 나타내는 各各 서로 相異한 分劃을 볼 수 있었으며 人體諸 臟器와大體로 類似하였으나 家兎 血淸의 ALP는 사람에서 와는 다르게 肝組織의 分劃과 一致하지 않고 오히려 骨組織과 더 잘 一致되는 것 같았다. ALPI 活性度에 있어서 家兎의 諸臟器는 生化學的 定量法으로 腎 ALP의 活性度가 49.5B.L. units로 다른 臟器組織에 비하여 顯著하게 높았으며, 그 다음은 脾臟, 脊椎骨, 肝, 肺淋巴腺 및 脾 順으로 높은 活性度를 나타내었으며 人體에 있어서는 胎盤, 骨, 小賜, 脾臟, 肝 및 腎臟의 順으로 높았다. 臟器 特異性 沮害因子에 對한 影響에 있어서 胎盤分劃은 特有하게 耐熱性을 나타내었고 보다 urea低抗性이며 肝分劃은 neuraminidase에 對하여 移動度의 遲延을 招來하였다. The studies upon cellulose acetate microzone electrophoretic patterns, quantitative estimation and effects of organspecific inhibitor on ALP isoenzyme of rabbit and human sera and various organ tissues were performed. On electrophoretic pattern the tissue ALP from the liver bone kidney lymph node spleen and intestine showed the characteristic fraction of high activities. The electrophoretic mobility of rabbit serum ALP was not correspond to the liver ALP as observed in human serum and it appeared to be rather corresponded to the bone ALP, which seemed to be the origin of ALP in serum of rabbit. In the quantitative measurement of tissue ALP activity the kidney ALP showed the highest level and the activity of the skeletal muscle ALP was the lowest(1.5 B. L. units), while higher activities were observable in placenta, bone, intestine, spleen, liver and kidney in order. On the effect of organ specific physico chemical agents the placental ALPI was highly stable to the heat and relatively resistant to urea while other organ ALPI were on the contrary. The neuraminidase seemed to effect to the motility of liver fraction.

퇴행성 경추질환의 전방 경유 경추부 수술시 내고정술 시행 유무에 따른 분석

정현재,조재훈,이성락,강동기,김상철 대한신경외과학회 1998 Journal of Korean neurosurgical society Vol.27 No.12

산부식 법랑질의 타액오염에 의한 bracket 결합력 효과에 관한 실험적 연구

김현덕,김정기,김오환 全北大學校 齒醫學硏究所 1993 전북치대논문집 Vol.11 No.1

The purpose of this study was to determine the effect of salivary contamination of etched enamel on shear bond strength of a bracket adhered to etched enamel. Eighty extracted human permanent premolars were used in this study. These samples were divided into two groups. Buccal surface of samples were etched in vitro with 38% phosphoric acid for 15 seconds and 60 seconds. Each group was divided into four subgroups. Etched enamel surfaces were contaminated with saliva for 0, 1, 20, 60 seconds, washed and dried. Test surfaces were examined using scanning electron microscope (SEM). The shear bond strength of each sample was determined with a universal testing instrument (Instron Co. Model 4201). Results were as follows. 1. Salivary contamination for 1, 20, 60 seconds did not affect shear bond strength when compared with the uncontaminated enamel group. 2. There was no significant difference (P>.05) in shear bond strength between 15 sec. and 60 sec. etching in uncontaminated enamedl groups. 3. When samples were examined using SEM, organic materials coated enamel surface masked the etched pattern partially.

인슐린종에 의한 측두엽 간질 발작 1례

최현경,김정기 대한신경정신의학회 2000 신경정신의학 Vol.39 No.2

저자들은 50대 여성에서 인슐린종에 의해 발생한 간질 발작에서 인슐린종의 제거 이후, 저혈당이 교정된 상태에서도 지속적인 이상 뇌파를 보인 예를 경험하였다. 환자는 간질 발작 이외에 저혈당의 일반적인 증상인 두통, 진전, 발한, 창백, 심계 항진 등을 거의 보이지 않아 오랫동안 측두엽 간질로 진단되어 항전간제를 사용하였다. 뇌파에서 좌측 측두엽에 이상을 보였고 인슐린종을 성공적으로 제거하고 저혈당이 교정되어 간질이 소실되었는데도 뇌파의 이상 소견이 지속되었다. 본 증례에서 혈당의 농도가 낮은데도 불구하고 환자가 전혀 저혈당의 증상을 느끼지 못하였다. 낮은 혈당에서도 glycosy Hb A1c의 농도가 정상에 가까워 뇌의 glucose uptake 가 유지되고 sympathoadrenal activation 과 counteregu-latory hormones으 반응이 감쇠되는 경우가 있다고 한다. 본 증례에서도 glycosy Hb A1c가 4.0%로 정상에 가까웠다. 저혈당에 의한 간질의 기전에 대하여서 간질의 역치를 감소시킨다는 설과 국소적인 뇌의 산소 소모를 감소시킨다는 설이 있어왔다. 최근에는 국소적인 뇌손상이 간질 유발의 원인이라는 설이 우세한데 excitotoxic mechanism으로 설명되고 있다. 증례에서 뇌파의 이상소견이 지속되었던 것은 국소적인 뇌 손상의 기전과 일치하는 결과로 생각되며 지속적인 뇌파의 추적 관찰이 필요할 것으로 여겨진다. Authors experienced a case of insulinoma which was initially misdiagnosed as idopathic tem-poral lobe epilepsy with automatism. This patients did not show other hypoglycemic symptoms except seizure. It has been reported that some of the patients with insulinoma are unaware of hypoglycemia and are at increased risk for seizures and coma. These patients would have normal glucose uptake in the brain and consequently no sympathoadrenal activation would begin, resulting in an awareness of hypoglycemia. It this case, abnomal EEG pattern consistent with a complex partial seizure remained after successful operation. Recent reports indicate that hypoglycemia is capable of killing neurons in the brain . An endogenous neurtoxin is produced and is released by the brain into tissue and cerebrospinal fluid. Endogenous excitotoxins produced during hypoglycemia may explain the tendency toward seizure activity often seen clinically. We suggest that persistent abnormalities of EEG in this case may be related to focal neuronal damage in hypoglycemia.

감단자(시단자)의 관능적 및 텍스쳐 특성

이효지,백현남,김정원 한국조리과학회 2004 한국식품조리과학회지 Vol.20 No.6

The objective of this study was to seek the best recipe for making Gamdanja. Gamdanja is a kind of rice cake made from persimmon extract and waxy flour, with a little salt mixed into the waxy flour. The method involved boiling the persimmon extract and beating in the waxy rice powder until all the waxy flour had been added.The procedure was as follows: Gamdanja containing different ratios of ingredients, such as persimmon extract (23, 23.5 and 24 hrs) and waxy rice flour (160 and 180g). The most desirable recipe was determined after sensory examination and mechanical tests using texture, moisture content and colorimeter measurements. The results of the sensory evaluation showed that Gamdanja made using persimmon extracted for 23.5 hrs, with the additional of 160g of waxy flour gave the higher scores for QDA profile, chewiness, delicacy and after swallowing preference than the other ratios. The results of the acceptance test also showed that the Gamdanja made using the persimmon extracted for 23.5 hrs with 160g of waxy rice flour was the best mix in every respect, such as for color and overall acceptability. In the textural analysis of Gamdanja, the hardness, adhesiveness, gununiness and chewiness were increased by the additiong of furtherwaxy flour. The moisture content of Gamdanja with persimmon extracted for 23 Ins was higher than those extracted for 23.5 and 24 hrs. The L-, a- and b-values of Gamdanja were increased with decreasing waxy flour. The sensory and mechanical examinations showed the overall quality of Gamdanja had positive correlations with delicacy and color, but negative correlations with hardness, adhesiveness, gumminess, and chewiness. As a result of these tests, the most desirable recipe for the Gamdanja was persimmon extracted for 23.5 hrs with the additional of 160g of waxy rice flour, containing I% salt and a moisture content of 45.87%.

대구경 볼트와 와이어텐션을 이용한 슬래브 보강공법에 관한 실험적 연구

서동기,노현섭,김상모,이창남,김상섭 대한건축학회 2003 대한건축학회 학술발표대회 논문집 - 계획계/구조계 Vol.23 No.1(구조계)

As time goes up, structural capacity of members decreases in reinforced concrete structures. The deterioration of the capacity is mainly due to the poor construction, corrosion of steel bars and the neutralization of concrete due to environmental pollution, etc. This deterioration is unavoidable, thus it is necessary to develop the method for repair and retrofit of structures in order to ensure structural safety of members. The purpose of this paper is to investigate the load-deflection, ultimate strength, failure mode, and energy absorption capacity of reinforced concrete slabs subject to load experimentally for several variables and grasp the problem of concrete slabs strengthened with large diameter bolts and wire tensioning. And propose foundational research data for4 strengthening reinforced concrete slabs. The test result shows that the capacity of anchorage is controlled by bearing capacity of concrete under the anchorage or punching shear around the anchorage. And the gap between bolts and core hole must be filled to prevent slip and rotation of anchorage.

Benzene 분해 미생물의 분리 및 특성

서현호,정병운,전기일,김형갑 진주산업대학교 환경문제연구소 1998 環境硏究論文集 Vol.2 No.-

Flavobacterium sp. BEN2 was selected and identified out of 64 benzene-degrading bacteria isolated from soils and waste waters by the enrichment culture technique. The optimal temperature, pH, and benzene concentration for the growth of Flavobacterium sp. BEN2 appeared to be 30℃, 7.0 and 2000 ppm, respectively. Cell growth almost revealed a stationary phase at 24 hours after cultivation and benzen was completely degraded during as benzen, benzoic acid, phenol, p-cresol, salicylic acid, catechol, gentistic acid and toluene, but did not grow ob o-cresol, m-cresol, p-xylene and p-toluic acid. With this strain, catechol was degraded through both meta- and ortho- cleavage pathway. Therefore, it is suggested that Flavobacterium sp. BEN2 could be effectively used for the biological treatment of wastewater containing benzene in the presence of some aromatic compounds and organic solvents.

PCB부품 자동삽입기의 효율적 삽입경로에 관한 연구

문기주,윤상섭,김상현 한국경영과학회 2000 한국경영과학회 학술대회논문집 Vol.- No.2

For miniaturization of electronic parts and higher productivity, part arrangement job of PCB(printed Circuit Board) has been replaced with complete automated facilities, and contribution which is concerned about this will be expanded continuously. Such part arrangement job of PCB could have lots of influences to entire productivity. In this paper, effective grouping method of parts is suggested for the effective insertion path job of PCB concerning the constraints to the size of parts.

물의 종류를 달리한 동치미의 경도 변화 및 세포벽 관찰

심영현,안기정,김지은 한국조리과학회 2004 한국식품조리과학회지 Vol.20 No.1

The purpose of this study was to investigate the changes of hardness and microstructure of Dongchimi cooked with various source of water(distilled water, purified water, Cho Jung Carbonated Natureal water). This study was conducted to observe the change of pH, total acidity, salt content, turbidity, texture and microstructure. Dongchimi cooked with source of water of water was fermented at 10℃ for 46 days. The changes of pH on Dongchimi cooked with various source of water decreased in all samples during fermentation period, and then showed a slowly decrease after 12 days of fermentation. The total acidity of Dongchimi cooked with Cho Jung Carbonated Natural water was arrived slowly at best tasting condition 0.3~0.4 point compared with other conditions. So Dongchimi cooked with Cho Jung Carbonated Natural water was continued to the best tasting condition for end of fermentation. At early stage of fermentation, the changes of turbidity of Dongchimi used Cho Jung Carbonated Natural water showed highly as compared with other test condition for 12th days of fermentation. The maximum cutting force of chinese radish of Dongchimi showed the highest value among all at the 25th day of ripening and then decreased gradually. The maximum cutting force of chinese radish of Dongchimi used Cho Jung Carbonated Natural water was the highest compared with other conditions at 25th day of fermentation. The calcium content of Dongchimi juice used Cho Jung Carbonated Natural water was observed high at the early stage of fermentation and showed the highest value at 25th day of ripening. The calcium content of chinese radish and Dongchimi juice of Dongchimi cooked with water purifier was lower than that of Dongchimi cooked with Cho Jung Carbonated Natural water, and was higher than that of Dongchimi cooked with Distilled water at the early stage of fermentation. The magnesium content in all samples increased gradually from the early stage of fermentation. The microstructure showed disintegration appearance of middle lamella and cell wall during fermentation period.

불량행위 청소년을 중심으로 한 학교폭력 가해자의 사회인구학적 특성 및 정신병리(V)

박지도,최현경,장우성,조동환,김정기 大韓神經精神醫學會 2001 신경정신의학 Vol.40 No.4

연구목적 : 이 연구는 불량행위 청소년군과 학생군을 대상으로 학교내외에서 행하는 폭력행동의 특징을 알아보고 특히 가해자의 정신병리와 청소년의 폭력행동과의 관계를 알아봄으로써 학교폭력의 이해와 대처방법 그리고 예방프로그램의 수립에 도움이 되고자 한다. 방 법 : 불량행위 청소년(청소년 선도프로그램인 BBS참석자) 남자 331명과 남자 중고등학교생 394명을 폭력의 유형을 괴롭힘, 금품갈취, 신체적 폭력, 언어적 폭력, 위협 등의 5가지 유형으로 나눈 설문문항을 바탕으로 각 경우의 경험빈도를 5점 척도로 하여 각 문항에서 3점 이상을 가해자군으로 분류하였으며 설문지를 통한 조사와 정신병리의 측정도구로서 한국판 아동-청소년 문제행동 평가 척도표(CBCL : Child Behavior Checklist, 청소년용)및 청소년의 자기개념을 알아보기 위해 한국판 피어스-해리스 소아 자기 개념척도(Piers-Harris Children's Self-Concept Scale, Piers, 1984, 이하 자기개념척도)를 적용하였으며 Chi-square test, T-test, 그리고 Two-way ANOVA를 사용하여 비교하였다. 결 과 : 사회 인구학적 특성에서는 대부분의 요인에서 불량행위군과 학생군 그리고 가해자군과 비가해군간에 차이는 보이지 않았으나 흡연과 음주하는 비율이 불량행위군과 학생군 두 군 모두 가해자군이 비가해자군보다 의미있게 높게 나타났다. 부모의 부부싸움 빈도는 불량행위군에서는 가해자와 비가해자군간에 차이를 보이지 않았으나 학생군에서는 가해자군에서 종종 싸운다는 비율이 의미있게 높게 나타났다. 학교생활에서는 성적이나 교우관계에서는 두 군 사이와 가해여부에 따라 유의한 차이를 보이지는 않았으며 성적은 불량행위군이 학생군에 비해 낮은 반면 가해여부에 따라서는 차이가 없었다. 하지만 학생군에서는 가해자군이 비가해자군보다 성적이 낮았으며 만화방을 이용하는 빈도도 가해자군에서 높게 나타났다. 폭력물 시청 시 느낌은 불량행위군과 학생군 모두에게서 가해자군이 비가해자군에 비해 흥분된다는 비율이 높게 나타났다. CBCL(Child Behavior Checklist)척도는 불량행위군의 경우 비행, 공격성, 외현화, 사회성 척도가 학생군에 비해 유의하게 높았고 위축, 사회적 미성숙, 사고문제, 주의집중, 내재화 척도는 학생군에서 불량행위군에 비해 유의하게 높았다. 전체 대상군을 가해자군과 비가해자군으로 나누어 비교하였을 때 신체증상, 사고문제, 주의집중, 비행, 공격성, 내재화, 외현화척도에서 가해자군이 비가해자군에 비해 유의하게 높았으며 군과 가해여부의 상호작용효과 검증결과에서는 유의한 상호작용을 보이는 척도는 없었으나 각 집단의 모든 척도에서 가해자군이 비가해자군보다 높은 점수를 보여주었으며 불량행위군의 경우 가해자군일수록 비행, 공격성, 외현화 척도가 높아지는 경향을 보였으며 학생군의 경우에서도 마찬가지로 비행, 공격성, 외현화 척도에서 가해자군이 비가해자군보다 높게 나타났다. 자기개념 척도는 불량행위군의 경우 행동, 지적학업척도에서 학생군에 비해 유의하게 낮게 나타났으며 불안, 인기척도는 학생군에서 불량행위군보다 유의하게 낮게 나타났다. 전체 대상군을 가해자군과 비가해자군으로 나누어 비교하였을 때 행동, 지적학업척도는 가해자군이 비가해자군에 비해 유의하게 낮은 점수를 보여주었으며 각 군과 가해여부의 상호작용검증결과에서는 유의한 상호작용효과를 보인 것은 없었으나 두 군 모두 행동척도가 가해자군이 비가해자군에 비해 낮은 경향을 보여주었다. 결 론 : 불량행위군과 학생군에서의 폭력가해자는 사회인구학적 변인에서는 비가해자와 유사한 특징을 가지나 각자의 내재된 심리상태와 정신병리에 따라 폭력 영상물과 음주 흡연의 가능성증가 그리고 가정에서의 폭력의 학습 등 주변의 위해 요인에 가해자군이 비가해자군에 비해 영향을 많이 받는 것으로 생각된다. 이에 학교폭력에 대한 대책수립 시 결과에만 초점을 맞춘 처벌 위주의 방식에서 벗어나 이들이 가진 심리적 특징과 정신병리를 이해하고 그에 따른 예방적인 대책수립이 필요할 것으로 생각된다. Objective : The purpose of this study was to identify the characteristics of school violence, self concept, psychopathology and psychosocial characteristics of male aldolescent delinquency doing school violence. And we hoped to use this result as basic material for prevention of school violence through comparison of those of students group. Method : We surveyed 725 male adolescents including 331 deliquents from educational program called "Big Brothers and Sister" in Pusan. We used self-reporting questionnaire composed of demographic data and attitude to violence. In order to investigate the psychopathology and self-concept, we used Child Behavior Check List, Youth edit, Korean version(CBCL) and the Piers-Harris Children's Self-Concept Scale. Statistical methods employed were Chi-square, t-test, two-way ANOVA. Result : The results show that in most demographic and psychosocial factor including age, education, socio-economic condition, religion and relationship with friends, there was no significant difference between delinquent and student group. And there was no significant difference between bully and non-bully in both group. The frequency of alcohol drinking and smoking were higher in bullying groups. For the feeling of watching violent mass-media, bullying delinquents reported more excited state than non-bullying delinquents in both group. On results of CBCL, subscales of delinquent behavior, aggressive behavior, externalizing problem, social scale in delinquent group were higher than those in student group. Subscale of somatic complaints, thought problem, attention problem, delinquent behavior, aggressive behavior, internalizing problem, and externalizing problem in bullying group were higher than those in nonbullying group. In both delinquent and student group, subscale of delinquent behavior, aggressive behavior, and externalizing problem in bullying group were significantly higher than those in nonbully group. In the results of self-concept scale, subscales of behavior, intellectual and school status in delinquent group were lower than those in student group. Subscale of somatic complaints, thought problem, attention problem, delinquent behavior, aggressive behavior, internalizing problem, and externalizing problem in bullying group were higher than those in nonbully group Conclusion : In both delinquent and student groups, most psychosocial factors in bully group were not significantly different with those in nonbully group. Bully group was more influenced by internal psychic state, psychopathology and environmental factor than nonbully group.