마우스의 BCG감염이 R.tsutsugamushi의 증식에 미치는 영향

김인재,전창덕,홍영권,정현택,이복수,장우현,김익상,박석돈 대한감염학회 1993 감염 Vol.25 No.2

It has recently been reported that gamma-inteferon(INF-r) and tumor necrosis factor(TNF) or lipopplysaccharide(LPS) activate macrophages to kill intracellular parasites by means of nitrc oxide (NO). It is now generally accepted that NO is the endothelium-derived relaxing factor, and that it also paticipates in the regulation of the nervous and immune systems. Activated macrophages form NO?? and NO?? from the terminal guanidino nitrogen atoms of L-arginine by the process now known to proceed via the formation of NO. This pathway is inhibited by the L-arginine analog(N?? monomethyl-L-arginine; N??MMA), forms L-citrulline as a co-product and is responsible for the cytotoxic action of macrophages. In this study, the authors found that INF-r and/or LPS induced murine macrophages to kill Rickettsiae tsutsugamushi in vitro and macrophages from BCG-infected mice got the host resistance against R.tsutsugamushi in vitro as well as in vivo. The rickettsicidal effect induced by the combination of INF-r and/or LPS induced mice got the host resistance against R.tsutsugamushi in vitro as well as in vivo. The rickettsicidal effect induced by the combination of INF-r and/or LPS can also be completely inhibited by N??MMA, leading to a dose-dependent inhibition of NO production. These datademonstrate that INF-r and/or LPS as well as BCG-activated macrophages mediate host resistance aganist R.tsutsugamushi infection through NO, which is necessary for the intracellular parasite.

사이토카인과 Lipopolysaccharide 자극에 의한 RAW 264.7 세포주의 nitric oxide 생성

김영덕,전창덕,이병순,이복수,박석돈,백상기,정헌택 충남대학교 생물공학연구소 1993 생물공학연구지 Vol.3 No.-

Macrophages have been implicated as a major class of effector in the host response to neoplasia. Cells of the monocyte-macrophage lineage are known to exhibit tumoricidal activity following stim-ulation by γ-interferon, tumor necrosis factor, BCG and bacterial products such as lipopoly-saccharide (LPS). While the mechanism involved remain obscure, the generation of reactive nitrogen intermediate (RNI) by activated macrophages is considered a maior participant in mediating the tumorstatic effect. But much of what is known about the induction and release of RNI has been elucidated by using freshly isolated cells from blood and other tissues of experimental animals. In this study, we used a murine macrophage cell line, RAW 264.7, and found that these cells showed above 99% positive of pan macrophage marker by immunohistochemical staining. These cells could produce nitric oxide (NO), when incubated with γ-IFN or poly I:C. Incubation of RAW 264.7 cells with γ-IFN for 48 hous in the presence of LPS agumented NO release in a dose dependent manner. Whereas, treatment of anti-TNE-α antibody or antisense TNF-α oligodeoxynucleotide inhibited the release of NO_2 by γ-IFN plus LPS activated macrophages. The production of NO was also inhibited reversibly or irreversibly by N^GMMA,NAA,arginase or DPI. Thease data suggest that RAW 264.7 cell line may be useful for the in vitro evalulation of biological response modifiers as well as the study of signal pathway of NO release by macrophages.

LCD의 회색 준위 상관 색온도 보정

최덕규,박차훈,김태선 경운대학교 산업기술연구소 2002 産業技術硏究論文誌 Vol.5 No.1(B)

At the present time, the LCD (liquid crystal display) not only emitsless radiation and consumes less power, it also takes up less space allowing for a more comfortable work environment. This paper looks into characteristics of LCD's CCT. We propose the LUT (look-up table) which adjust the three primary colors'digital input value, for maintaining the fixed value of correlated color temperature. LCD's CCT is maintaining about 6500 K, which is the standard CCT of display systems. Moreover it is the same as the former LCD's luminance that LCD's with using the LUT. In addition, we confirm that the color reproducibility of LCD using the proposed LUT become much accurate than a conventional LCD.

SMD 자동화 공정의 부품 혼입 방지 시스템

최덕규,박차훈,김태선 경운대학교 산업기술연구소 2002 産業技術硏究論文誌 Vol.5 No.1(B)

SMD (surface Mount Device : Print된 기판에 부품을 장착)공정은 완전 자동화 시스템이며, 이 작업을 진행하기 위하여 백 수 십여 가지의 부품을 정해진 부품 카트리지에 삽입한 후 자동화 설비는 그에 맞는 부품을 장착하게 된다. 그러나 부품을 카트리지에 삽입하는 작업을 작업자가 하게 되는데 이는 사람이 하는 일이라 Human Error가 발생할 가능성이 높다. 본 논문에서는 Chip Mounter에 센서를 부착하여 카트리지의 탈착 여부를 실시간으로 감시하는 모니터링 시스템과 부품 카트리지에 표시된 정보를 Bar-code Reader로 판독하게 함으로서 SMD Chip 장착 자동화 공정의 부품 혼입 방지 시스템을 개발하였다. 개발한 장비를 현장에 투입, 실 생산활동에 활용하게 하여 해당기업의 생산성 향상에 크게 기여하고 있다.

생체신호 처리용 지능형 ASIC 칩 개발

박차훈,최덕규,김태선 경운대학교 산업기술연구소 2002 産業技術硏究論文誌 Vol.5 No.1(A)

The proposed enhanced telemetry IC is verified with Altera FPGA chip (EPF10K20RC240-4) and layout with 0.6㎛ CMOS n-well process technology. For the VLSI design, a high level language was used, decreasing layout time by a factor of four. They resulting design, performed with a combination of Synopsys behavioral and structural code. The digital simulator used was VHDL logic simulator running under MaxplusⅡ.

신생아 말초혈액 T 림프구의 아형 분석

최두영,김종덕,장선일,이신은,조성경,정헌택 최신의학사 1990 最新醫學 Vol.33 No.12

신우신염으로 오인된 부종양증후군을 동반한 신세포암

한민철,김재헌,박성재,변동원,강덕희,심봉석 이화여자대학교 의과대학 2004 EMJ (Ewha medical journal) Vol.27 No.2

Renal cell carcinoma can presents wide range of signs and symptoms, and commonly associated with paraneoplastic syndrome. Paraneoplastic manifestations are present in up to 20% of patients with renal cell carcinoma. There is convincing evidence that renal cell carcinoma tumor cells elaborate proteins that serve as mediators of endocrine (ex ; ectopic production of parathyroid hormone-related protein or erythropoietin) as well as nonendocrine paraneoplastic syndromes. A paraneoplastic syndrome may be the various clinical presentation of renal cell carcinoma in a significant number of patients, therefore mimicked other general disease. We report a case of renal cell carcinoma associated with paraneoplastic syndrome mimicking pyelonephritis.

급성 심근경색후 생존심근 진단에서 Dobutamine 심초음파도와 Thallium 심근스캔의 정확성 비교

이근,강덕현,송재관,이철환,최기준,홍명기,김재중,박성욱,박종훈,박승정 대한순환기학회 1998 Korean Circulation Journal Vol.28 No.2

전류제어형 PWM 인버어터―유도전동기 위치제어의 신경회로망 응용

元忠淵,梁承浩,金世燦,金德憲 成均館大學校 科學技術硏究所 1991 論文集 Vol.42 No.1

이 논문은 전력변환기 시스템 모델링에 대한 신경회로망 사용을 기술하였다. Bp 알고리즘은 PWM 인버어터에 의해 구동되는 유도전동기의 on-off 패턴제어를 모델화하기 위해 처음으로 사용되었다. 신경회로망의 한 잇점은 본질적으로 병렬처리 이어서 그 결과 직렬인 디지털 컴퓨터보다 훨씬 빠르다. 게다가 신경회로망은 인버어터에 대해 학습능력이 있다. 제안된 방법의 타당성을 디지털 컴퓨터 시뮬레이션을 통하여 확인하였다. This paper discribes the use of neural networks for modeling power converter system. A back propagation(BP) algorithm is used firstly to model on-off pattern control of PWM inverter fed induction motor and, as a result, can solve problems much faster than a serial digital computer. Furthermore, neural networks have ability to learn for inverter. The validity of proposed method is confirm through the digital computer simulation.

미세혈관 협심증 환자의 임상상 및 지표에 관한 연구

박상호,온영근,김현정,이태훈,길효욱,김현건,박수진,송해정,한대희,방덕원,신원용,이내희,김성구,권영주 순천향의학연구소 2003 Journal of Soonchunhyang Medical Science Vol.9 No.1

Background : Microvascular angina or syndrom X is heterogenous and encompasses different pathogenetic entities. Several studies suggest that microvascular angina have good prognosis and occurs predominantly in postmenopausal women. Lipoprotein (a), total cholesterol, LDL-cholesterol, HDL-cholesterol, triglyceride, homocystein, CRP and fibrinogen are the independent risk factor of acute coronary syndrome and are the chief underlying cause of atherosclerosis. We investigated clinical characteristics and markers in patients with microvascular angina. Methods : We studies 23 patients diagnosed with microvascular angina and 16 control group with negative treadmill exercise test and no significant coronary angiogram. We investigated clinical characteristics in patients group and evaluated symptom during a follow-up period of 4.2± 2.5 month. Exercise time in treadmill exercise test was compared between patients and control group. We assessed the level of markers, such as lipoprotein (a), total cholesterol, LDL-cholesterol, HDL-cholesterol, triglyceride, homocystein, CRP and ESR in two groups. Results : There is no difference in age between two groups. Patients group had a tendency to increase in female, hypertension, DM, and smoking history, but there was no statistical significance difference. Exercise duration time was significantly decreased in patients with microvascular angina (p<0,05). Serum lipoprotein (a), total cholesterol, LDL-cholesterol, HDL-cholestrol, Triglyceride, ESR, Fibrinogen and Homocystein value were no significant difference between microvascular angina and control group. Only CRP was significantly high in patients with microvascular angina (p<0.05). Conclusion : There was no statistical significance difference in age, sex, hypertension, DM and smoking history between two groups but patients group had a tendency to increase the prevalence in hypertension, DM and smoking history, female. Also, Symptom improvement and disappearance in follow-up was presented in 11 patients of 19(57.9%). Exercise tolerance was worse in patients group than control group. CRP value was significantly higher in patients group than control group. There was no statistical significance difference in lipoprotein (a), total cholesterol, LDL-cholesterol, HDL-cholesterol, triglyceride, homocystein, ESR and Fibrinogen between two groups.