Vascular regeneration and skeletal muscle repair induced by long-term exposure to SDF-1α derived from engineered mesenchymal stem cells after hindlimb ischemia

Kim Jin-Ju,Park Jae-Hyun,Kim Hyeok,Sim Woo-Sup,Hong Seokbeom,Choi Yeon-Jik,Kim Hyo-Jin,Lee Soon Min,Kim Dongha,Kang Sun-woong,Ban Kiwon,Park Hun-Jun 생화학분자생물학회 2023 Experimental and molecular medicine Vol.55 No.-

Despite recent progress in medical and endovascular therapy, the prognosis for patients with critical limb ischemia (CLI) remains poor. In response, various stem cells and growth factors have been assessed for use in therapeutic neovascularization and limb salvage in CLI patients. However, the clinical outcomes of cell-based therapeutic angiogenesis have not provided the promised benefits, reinforcing the need for novel cell-based therapeutic angiogenic strategies to cure untreatable CLI. In the present study, we investigated genetically engineered mesenchymal stem cells (MSCs) derived from human bone marrow that continuously secrete stromal-derived factor-1α (SDF1α-eMSCs) and demonstrated that intramuscular injection of SDF1α-eMSCs can provide long-term paracrine effects in limb ischemia and effectively contribute to vascular regeneration as well as skeletal muscle repair through increased phosphorylation of ERK and Akt within the SDF1α/CXCR4 axis. These results provide compelling evidence that genetically engineered MSCs with SDF-1α can be an effective strategy for successful limb salvage in limb ischemia.

KDM3A histone demethylase functions as an essential factor for activation of JAK2−STAT3 signaling pathway

Kim, Hyunkyung,Kim, Dongha,Choi, Seon Ah,Kim, Chang Rok,Oh, Se Kyu,Pyo, Ki Eun,Kim, Joomyung,Lee, Seung-Hoon,Yoon, Jong-Bok,Zhang, Yi,Baek, Sung Hee National Academy of Sciences 2018 PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF Vol.115 No.46

<P><B>Significance</B></P><P>An aberrant covalent histone modification which drives dysregulation of transcriptional program is related to human diseases such as cancer. Thus, identifying signaling pathways modulating transcription factors and epigenetic enzymes are coming into view as attractive therapeutic targets. In this study, we provide evidence that KDM3A is tyrosine-phosphorylated by JAK2, and tyrosine-phosphorylated KDM3A acts as a coactivator for STAT3, thereby exerting increased cancer cell growth and motility. We propose that JAK2-dependent tyrosine phosphorylation of KDM3A could be a potential therapeutic target for epigenetic control of oncogenic effect governed by JAK2−STAT3 signaling pathway.</P><P>Janus tyrosine kinase 2 (JAK2)−signal transducer and activator of transcription 3 (STAT3) signaling pathway is essential for modulating cellular development, differentiation, and homeostasis. Thus, dysregulation of JAK2−STAT3 signaling pathway is frequently associated with human malignancies. Here, we provide evidence that lysine-specific demethylase 3A (KDM3A) functions as an essential epigenetic enzyme for the activation of JAK2−STAT3 signaling pathway. KDM3A is tyrosine-phosphorylated by JAK2 in the nucleus and functions as a STAT3-dependent transcriptional coactivator. JAK2−KDM3A signaling cascade induced by IL-6 leads to alteration of histone H3K9 methylation as a predominant epigenetic event, thereby providing the functional and mechanistic link between activation of JAK2−STAT3 signaling pathway and its epigenetic control. Together, our findings demonstrate that inhibition of KDM3A phosphorylation could be a potent therapeutic strategy to control oncogenic effect of JAK2−STAT3 signaling pathway.</P>

Copper-Catalyzed Selective Arylations of Benzoxazoles with Aryl Iodides

Kim, Donghae,Yoo, Kwangho,Kim, Se Eun,Cho, Hee Jin,Lee, Junseong,Kim, Youngjo,Kim, Min American Chemical Society 2015 Journal of organic chemistry Vol.80 No.7

<P>A copper-catalyzed direct ring-opening double N-arylation of benzoxazoles with aryl iodides has been developed. The present system exhibits high selectivity despite competition from C-arylation. The selectivity between ring-opening N-arylation and C-arylation was controlled by the choice of reaction vessel. The nitrile bound bis(triphenylphosphine)copper cyanide was identified as the active catalytic species for both reactions, and when combined with a nitrile-containing solvent, enhanced the reaction efficiency.</P>

폐플라스틱 열분해 산업: 현황과 전망

김동해(Donghae Kim),한명훈(Myeonghun Han),김나현(Nahyun Kim),김지현(Jihyeon Kim),정석희(Sokhee P. Jung) 대한환경공학회 2024 대한환경공학회지 Vol.46 No.7

열분해는 폐플라스틱을 분해하여 열분해유를 생산할 수 있는 기술로서, 다른 기술로 처리하기 불가능한 오염된 폐플라스틱도 처리할 수 있는 장점이 있다. 폐플라스틱 발생량이 증가하고, 화석연료와 같은 천연자원이 부족한 우리나라에서 열분해 기술은 ‘도시유전’이라는 키워드로 주목받고 있다. 대한민국 환경부에서는 폐플라스틱의 열분해 처리 비중을 2021년 기준 0.1%에서 2030년 10%까지 높이는 ‘폐플라스틱 열분해 활성화 방안’을 발표했다. 열분해의 생성물은 연료와 플라스틱 원료 두 가지이지만, 처리 과정 중 상당한 에너지를 소비한다. 그래서 열분해 기술의 시장성과 경제성이 어둡다는 지적이 지속되고 있기에, 친환경성에 대한 의문이 제기되고 있다. 이런 배경에서, 본 연구에서는 열분해 기술의 국제 동향을 조사하고, 환경적 측면에서 다른 기술과 상세한 비교 분석을 수행하였다. 본 연구 결과에 따르면, 현재 환경 선진국에서는 열분해유를 연료로 사용하는 것에 매우 부정적이며, 이에 다음과 같은 정책 방향을 제시한다. 1) 플라스틱 재생원료 의무 사용 비율을 준수함과 동시에 친환경성을 달성하기 위해, 열분해 기술을 연료화 보다 플라스틱 재생 원료 생산 기술로 사용함을 권장한다. 2) 열분해가 물리적 재활용에 비해 더 많은 온실가스를 배출하므로, 열분해 기술을 물리적 재활용이 어려운 폐플라스틱을 처리하는, 물리적 재활용을 보완하는 방법으로 사용한다. 3) 폐플라스틱의 자원순환에 있어 물리적 재활용이 매우 중요하므로, 분리 선별 기술의 발전을 국가적으로 도모한다. Pyrolysis is a technology that can produce pyrolysis oil by decomposing waste plastic, and has the advantage of being able to process contaminated waste plastic that is impossible to process with other technologies. In Korea, where the amount of waste plastic is increasing and natural resources such as fossil fuels are lacking, pyrolysis technology is attracting attention under the keyword ‘urban oil field’. The Ministry of Environment of the Republic of Korea announced a ‘plan to revitalize waste plastic pyrolysis’ to increase the proportion of waste plastic pyrolysis treatment from 0.1% in 2021 to 10% in 2030. The products of pyrolysis are both fuel and plastic raw materials, but the processing consumes significant energy. Therefore, as criticism continues that the marketability and economic feasibility of pyrolysis technology is poor, questions are being raised about its environmental friendliness. Against this background, this study investigated international trends in pyrolysis technology and performed a detailed comparative analysis with other technologies from an environmental perspective. According to the results of this study, currently environmentally advanced countries are very negative about using pyrolysis oil as fuel, and the following policy directions are suggested. 1) In order to comply with the mandatory use ratio of plastic recycled raw materials and at the same time achieve eco-friendliness, it is recommended to use pyrolysis technology as a plastic recycled raw material production technology rather than fuel conversion. 2) Since pyrolysis emits more greenhouse gases than physical recycling, pyrolysis technology is used as a complementary method to physical recycling to process waste plastics that are difficult to physically recycle. 3) Since physical recycling is very important in the resource circulation of waste plastic, the development of separation and sorting technology is promoted nationally.

동축반전 프로펠러 직경 차이에 따른 추력 변화 분석

김동해(Donghae Kim),방영웅(Yeongwoong Bang),이동규(Donggyu Lee),김시원(Siwon Kim),김태종(Taejong Kim),안존(Jon Ahn) 한국항공우주학회 2023 한국항공우주학회 학술발표회 논문집 Vol.2023 No.11

최적의 필기노트 어플리케이션을 구성하는 기능 요소들

김동하(Dongha Kim),김민정(Minjeong Kim),김희수(Heesu Kim),양동준(Dongjoon Yang),임승록(Seungrok Lim),김효선(Hyosun Kim) 한국HCI학회 2013 한국HCI학회 학술대회 Vol.2013 No.1

태블릿 PC에서 활용되는 "필기노트" 어플리케이션의 사용성을 향상시키고자 인터뷰와 설문을 실시하였다. 인터뷰와 설문을 통해서 필기노트 어플리케이션의 필기모드에서 사람들이 선호하는 기능 제공 방식과 그 이유들을 분석 하였다. 본 연구의 결과는 필기노트 어플리케이션 제작 시 고려해야할 사항을 발견할 수 있다는 것에 의의가 있으며, 향후 해당 어플리케이션의 사용성 발전에 도움이 될 것이다. In this paper, the result of survey and interview provides suggestions to improve usability for "Writing application" using tablet PC. Through this interview and survey, we found out the preferable method of providing different functions when using writing application. Through this analysis, what to be considered before designing writing application is presented in details and it can be considered when improving usability.

금오공과대학교 메타버스 체험을 위한 Unity 기반의 플랫폼 개발

김준용(Junyong Kim),김동하(Dongha Kim),김창우(Changwoo Kim),정상경(Sanggyeong Jung),한선재(Seonjae Han),김성영(Sungyoung Kim) 한국정보기술학회 2023 Proceedings of KIIT Conference Vol.2023 No.6

Bc13-dependent stabilization of CtBP1 is crucial for the inhibition of apoptosis and tumor progression in breast cancer

Choi, Hee June;Lee, Ji Min;Kim, Hyunkyung;Nam, Hye Jin;Shin, Hi-Jai R.;Kim, Dongha;Ko, Enyoung;Noh, Dong-Young;Kim, Keun II;Kim, Jung Hwa;Baek, Sung Hee Sookmyung Women's University Research Institute of 2011 여성과 건강 Vol.6 No.2

B-cell lymphoma 3 (Bc13) is a proto-oncogene upregulated in a wide range of cancers, including breast cancer. Although Bc13 is known to promote cell proliferation and inhibit apoptosis, the molecular mech¬anisms underlying the proto-oncogenic function of Bc13 have not been completely elucidated. To gain insight into the oncogenic role of Bc13, we applied a proteomic approach, which led to the identification of C-terminal binding protein 1 (CtBP1) as a binding partner of Bc13. A PXDLS/R motif embedded in Bc13 was found to mediate the interaction between Bc13 and CtBP1, which caused the stabilization of CtBP1 by blocking proteasome-dependent degradation. Apoptotic stimuli-induced degradation of CtBP1 was sig¬nificantly abolished by the upregulation of Bc13, leading to the sustained repression of pro-apoptotic gene expression and subsequent inhibition of apoptosis. Intriguingly, a strong positive correlation between the protein levels of Bc13 and CtBP1 was detected in breast cancer patient samples. Our study reveals a novel combinatorial role for Bc13 and CtBP1, providing an explanation for the acquisition of resistance to apop¬tosis in cancer cells, which is a major requirement for cancer development.

Ellagic Acid Prevents Binge Alcohol-induced Gut Leakiness and Hepatic Damage via Regulating Gut Microbiota

Dongha Kim,Yejin Sim,Jin-hyeon Hwang,Young-Eun Cho 한국식품영양과학회 2020 한국식품영양과학회 학술대회발표집 Vol.2020 No.10

The factors influenced to the work efficiency

Kim, Dongha 江原大學校附設體育科學硏究所 1989 江原大學校附設體育科學硏究所論文集 Vol.- No.14

본 연구는 일의 효율과 관계가 있는 생체역학적 요인들을 알아보기 위하여 핀란드의 활동적인 여자 16명을 대상으로 하였다. 이들을 하지와 상지의 두 훈련 집단으로 나누어 근순발력 훈련을 16주와 12주 동안 실시하였다. 그러나 실험은 상지 훈련 집단도 하지 후련 집단과 같은 실험을 받았다. 훈련전에 모든 피험자들은 1차 실험은 받았으며, 16주후 2차실험을 1차 실험과 똑 같은 실험조건에서 받았다. 하지 훈련 집단은 훈련후 8주에 3차 실험을 받았으며, 상지 훈련 집단은 2차 실험시 하지의 굴신 운동시 슬관절각을 달리하는 실험 조건에서 또 한번의 실험을 받았다. 실험 내용은 최대 근순발력 검사와 100번의 굴신운동을 통한 피로 반응 검사를 받았다. 본 연구에서 분석한 영역은 근수축시 근육의 수축 길이의 장단이 일효율에 미치는 영향과, 근순발력훈련이 지속적인 운동시의 일효율에 미치는 영향과, 동작의 생체 역학적 변인들이 일효율에 미치는 영향으로 구분하였다. 분석 결과는 운동시 근수축의 길이가 짧은 무릅 관절각 110도의 실험조건에서 긴 실험 조건(90도)에서 보다 높게 나타 났으며 (24.54±2.3%와 22.85±2.6%), 근순발력 훈련후 지속적인 운동에서는 훈련전 보다 일효율이 낮아졌다(21.68±1.4%와 23.49±3.1%). 그러나 통계적 유의성은 검증되지 않았다. 일효율과 운동시 각 변인들과의 상관 관계 검증에서는 16개 변인중 6개 변인들 즉 최대 운동능력(MP)과, 총운동량(TW)은 통계적으로 유의성(P<0.05 이상)이 있는 정적 상관율, 연장 수축기와 단축 수축기의 접지시간(CTe and c)과, 발목과 무릎관절사이의 최대굴곡시간(AKmax)과 최대산소 섭취량(VO₂/㎏)에서 통계적으로 유의한(P<0.05 이상) 부적상관을 나타내었다. 이들 결과들은 근육의 연장 수축시 생성된 탄성력을 재이용하는 것이 일효율을 높이는 중요한 관건이며, 근지구력과 근력의 발현 능력이 또한 굴신 운동시 높은 일효율을 가져오는데 중요한 요인임을 제시하였다.