간내 담석과 동반된 간내 담도암에서의 p53과 Ki-ras의 발현

김명환,이성구,서동완,이승규,남승우,박능화,민영일,김연석,심기남,공경엽 대한소화기학회 1997 대한소화기학회지 Vol.30 No.5

Background/Aims: Hepatolithiasis or primary intrahepatic stones may be accompanied later by intrahepatic cholangiocarcinoma. This cancerous lesion is frequently associated with atypical hyperplastic epithelium that was suspected of being precancerous. To investigate the Ki-ras or p53 mutation may play a role in carcinogenesis, and to determine whether atypical hyperplastic epithelium may be precancerous, this study was performed in intrahepatic cholangiocarcinomas associated with hepatolithiasis. Methods: We examined 12 patients with intrahepatic cholangiocarcinomas associated with hepatolithiasis. Ki-ras point mutations were assessed by direct DNA sequencing. Expressions of p53 protein were immunohistochemically assessed. The overexpression of p53 and point mutation of Ki-ras were examined in normal, hyperplastic, atypical hyperplastic, and can- cerous tissues separately. Results: The expressions of p53 protein were detected in 4 (33%) of 12 cholangiocarcinomas and Ki-ras point mutations at codon 12 were found in 2 (17%) of 12 cholangiocarcinomas. In those two carcinoma cases which contained the mutant sequence of Ki-ras, the same point mutation from wild type GGT (glycine) to GAT (aspartic acid) was. found in the associated atypical hyperplastic epithelium. However, none of the normal and hyperplastic epithelium harbored Ki-ras or p53 mutations. Conclusions: The overexpression of p53 may play a part in the carcinogenesis of some intrahepatic cholangiocarcinomas associated with hepatolithiasis, whereas the role of Ki-ras mutations in those cases is dubious. However, Ki-ras point mutation at codon 12 may be responsible for either cancer or atypical hyperplastic epithelium associated with hepatolithiasis in certain cases, suggesting atypical hyperplastic epithelium may give rise to carcinoma.

Tetrahydroisoquinoline Alkaloids가 쥐의 동맥과 뇌균등질의 α-Adrenoceptors에 미치는 영향

이현석,백선하,황수현,박인성,김은상,정진명,한종우,장기철,장기철 대한신경외과학회 1999 Journal of Korean neurosurgical society Vol.28 No.1

정신분열병에 대한 리스페리돈의 효과 및 안정성

이민수,김용구,김영훈,연병길,오병훈,윤도준,윤진상,이철,정희연,강병조,김광수,김동언,김명정,김상훈,김희철,나철,노승호,민경준,박기창,박두병,백기청,백인호,손봉기,손진욱,양병환,양창국,우행원,이정호,이종범,이홍식,임기영,전태연,정영조,정영철,정인과,정인원,지익성,채정호,한상익,한선호,한진희,서광윤 大韓神經精神醫學會 1998 신경정신의학 Vol.37 No.1

연구목적 : 본 시험의 목적은 임상시험 시작전에 연구자들을 대상으로 PANSS Workshop을 통하여 PANSS, ESRS에 대한 국내에서의 표준화 작업을 구축하고 새로운 정신병 치료제인 리스페리돈의 효과와 안정성을 재확인하여 리스페리돈 사용에 대한 적정화를 이루는데 있다. 연구방법 : 1996년 4월부터 1996년 9월까지 국내 39개 대학병원 정신과에 입원중인 혹은 증상이 악화되어 입원하는 정신분열병 환자 377명을 대상으로 다시설 개방 연구를 시행하였다. 1주일간의 약물 배설기간을 가진후, 리스페리돈을 8주간 투여하였고, 기준점, 1주, 2주, 4주, 그리고 8주후에 평가되었다. 용량은 제1일에는 리스페리돈 1mg씩 1일 2회, 제2일에는 2mg씩 1일 2회, 제3∼7일에는 3mg씩 1일 2회 투여하였다. 이후 환자의 임상상태에 따라 임의로 증량할 수 있으며, 최대 일일 16mg을 초과하지 않도록 하였다. 추체외로 증상을 조절하기 위한 투약을 허용하였다. 임상증상 및 부작용의 평가는 PANSS(Positive and Negative Syndrome Scale), CGI(Clinical Global Impression) 그리고 ESRS(Extrapyramidal Symptom Rating Scale)을 사용하였다. 연구결과 : 377명중 343명(91%)이 8주간의 연구를 완결하였다. 치료 종결시점인 8주후 PANSS 총점수가 20% 이상 호전된 경우를 약물 반응군으로 정의할때, 약물반응군은 81.3%였다. 리스페리돈에 반응하는 예측인자로는 발병연령, 이전의 입원 횟수, 유병기간이 관련 있었다. 리스페리돈은 1주후부터 PANSS양성, 음성, 및 일반정신병리 점수상에 유의한 호전을 보여 효과가 빨랐다. CGI의 경우도 기준점에 비해 1주후부터 유의한 감소를 나타내었다. ESRS의 경우, 파킨슨 평가점수는 기준점과 비교해 투여 1주, 2주, 4주후 유의하게 증가되었다가 8주후 기준점과 차이가 없었다. Dystonia 평가점수는 1주후만 유의한 증가를 보였으며, dyskinesia 평가점수는 유의한 차이가 없었다. 혈압, 맥박수의 생명징후 및 일반 혈액학 검사, 생화학적 검사, 심전도 검사에서 유의한 변화는 없었다. 결 론 : 이상의 다시설 개방 임상 연구를 통해 리스페리돈은 정신분열병 환자에서 양성증상뿐만 아니라 음성증상 및 전반적인 증상에도 효과적인 것으로 사료된다. 보다 명확한 평가를 위해서는 다른 항정신병약물과의 이중맹검 연구가 필요할 것으로 생각되며, 또한 장기적 치료에 대한 평가도 함께 이루어져야 하겠다. Objective : The purpose of this study was to investigate the efficacy and safety of risperidone in the treatment of Korean schizophrenic patients. Method : This multicenter open study included 377 schizophrenic patients drawn from 39 university hospitals. After a wash-out period of 1 week, the schizophrenic patients were treated with risperidone for 8 weeks and evaluated at 5 points ; at baseline, and 1, 2, 4 and 8 weeks of treatment. The dose was increased from 2mg/day(1mg twice daily) to 6mg/day(3mg twice daily) during the first week and adjusted to a maximum of 16mg/day over the next 7 weeks according to the patient's clinical response. Medication to control extrapyramidal symptoms was permitted. The psychiatric and neurological status of the patients was assessed by PANSS, CGI, and ESRS scales. Results : 343(91%) of 377 patients completed the 8-week trial period. Clinical improvement, as defined by a 20% or more reduction in total PANSS score at end point, was shown by 81.3% of patients. The predictors of response to risperidone were associated older age, shorter duration of illness, fewer previous hospitalization. Risperidone had rapid onset of action ; a significant decrease of the total PANSS and three PANSS factor(positive, negative, general), and CGI was already noticed at the end of first week. For the ESRS, parkinsonism rating scores were significantly increased until week 4 comparing with baseline. Dystonia rating scores were significantly increased until week 1, and dyskinesia rating scores were not significantly changed during the study. Laboratory parameters including vital sign, EKG, hematological, and biochemical values showed no significant changes during the trial. Conclusions : This study suggests that risperidone is generally safe and effective against both the positive and negative symptoms in our group of patients.

정신과 입원환자의 병원이탈에 대한 고찰

기문상,박상학,김명규 大韓神經精神醫學會 1996 신경정신의학 Vol.35 No.2

This study was a survey of all available hospital records pertaining to the 300 elopers of psychiatric inpatients admitted at Naju National Mental Hospital during the years between 1991-1993. The findings were compared with randomly selected control group of 300 inpatients who admitted during the same period. This study was designed to evaluate the demographic data and clinical characteristics, motivations, and results of elopement in these patients. Results were as follows : 1) During 3 years, 308 of 4670 inpatients(6.5%) committed elopememt. 2) Most of them were involuntarily admitted young male patients and they were likely to be unmarried, unemployed, and high-school graduated compared to control group. Their socioeconomic status were relatively low. 3) While the control group was mainly diagnosed as undifferentiated schizophrenia, more of elopers was diagnosed as paranoid schizophrenia. 4) Clinically, elopers had chronic course of illness, frequent and long-term hospitalization, and previous history of elopement than control group. 5) Patients in open wards showed more frequent elopement behavior than those in closed wards. They were likely to elope during the strolling. Many attempted to elope on Thursday when the family members visited them. 6) Most of the elopers were safely returned to the hospital and the management was simple interview without restraint. Many of families were not unconcerned about the patient's elopement. 7) Elopement group were characterized by their severe psychotic symptoms(hallucination, delusion). poor relationship with treatment teams, and cold familial concerns. It was suggested that the patient's intrapsychic factor(42.8%) was the most important factor in determining the elopement.

黃化水素化合物이 紫外線B를 照射한 黑色마우스의 表皮멜라닌細胞의 形態에 미치는 影響

明基範,鞠泓一 梨花女子大學校 韓國生活科學硏究院 1989 韓國生活科學硏究院 論叢 Vol.44 No.-

C57BL 흑색마우스에 UVB를 매일 100mJ/cm^2씩 10일간 조사하여 표피멜라닌세포를 활성화시킨 후 5% cysteine과 5% glutathione이 표피멜라닌세포의 수적 변화와 멜라닌소체 형태변화에 미치는 영향을 관찰하여 다음과 같은 결과를 얻었다. 1. Cysteine 투여군에서는 멜라닌세포의 수적변화가 없었으며 glutathione투여 5주 후에서만 멜라닌세포의 수가 감소하였다. 2. Cysteine 투여군과 glutathione 투여군은 대조군에 비해 표피멜라닌세포내 멜라닌소체의 단축 길이와 stage Ⅳ 멜라닌소체 비율에 유의한 감소를 보여 멜라닌형성 억제제로서 의의있는 효과를 나타내었다. 3. 두 실험군간의 비교에서 멜라닌소체 단축길이는 glutathione 투여군에서 투여 3주 후에 cysteine 투여군보다 의의있게 감소되었으며, stage Ⅳ 멜라닌소체의 비율은 glutathione 투여군에서는 투여 3주 후에 cysteine 투여군에서는 투여 5주 후에 각각 유의한 감소소견을 보였다. 4. Glutathione 투여군에서는 멜라닌소체 단축길이와 stage Ⅳ 멜라닌소체의 비율이 모두 투여기간에 비례하여 감소되었다. 이상과 같은 결과로 황화수소화합물인 cysteine과 glutathione은 자외선 B조사로 활성화된 표피멜라닌세포내 멜라닌소체의 단축길이와 stage Ⅳ 멜라닌소체의 비율을 감소시킴으로써 멜라닌형성을 억제하고, glutathione이 cysteine보다 멜라닌형성억제에 우수한 효과를 나타냄을 알수 있었다. The effects of sulfhydryl compounds, cysteine and glutathione, on populations of melanocytes, the sizes of melanosomes and the ratios of melanosomal stages of epidermal melanocytes in UVB-irradiated C57BL black mice were evaluated. The results were as follow: 1. Both of cysteine and glutathione showed no significant diminution in the numbers of dopa-positive melanocytes except at the end of 5 weeks' treatment with glutathione. 2. Both of cysteine and glutathione showed significant diminution in short axis of melanosomes and the percentage of stage Ⅳ melanosomes of epidermal melanocytes. 3. The lengthes of short axes of melanosomes in glutathione-treated group were smaller than those in cysteine-treated group at the end of 3rd week of intraperitoneal injection. 4. In the glutathione-treated group, the short axes of melanosomes and the percentages of stage Ⅳ melanosomes decreased in proportion to the period of intraperitoneal injection.

연령증가에 따른 흰쥐 삼차신경절 및 삼차신경감각핵에서의 Neuropeptide Y 면역반응세포의 분포에 관한 연구

김명국,백기석,이승표 대한해부학회 1996 Anatomy & Cell Biology Vol.29 No.6

표피종양의 p27 발현에 관한 연역조직화학적 연구

이명기,정병수 조선대학교 2001 The Medical Journal of Chosun University Vol.26 No.1

Background and objectives: p27 belong to the family of cyclin dependent kinase inhibitors, which are important negative regulators of the cell cycle. Loss of normal cell cycle regulation contributes to the development and progression of tumors. Although p27 may play role in tumor progression, the precise function and expressive pattern in epithlial tumors are less clear. The propose of this study was to investigated the expressive patterns of p27 in benign, precancerous and malignant epithelial tumors. Materials and Methods: Formalin-Fixed and paraffin-embedded tissue from 5 seborrheic keratosis, 5 actinic keratosis, 5 Bowen disease, 7 basal cell carcinoma, 13 squamous cell carcinoma were immunolabeled with monoclonal antibodies directed p27. Results: The seborrheic keratosis showed a very low expression of p27 in all cases. In two cases of actinic keratosis, p27 staining was observed in dysplastic cells that proliferative into dermis. The Bowen’s disease showed low expression of p27 except dyskeratotic cells. The basal cell carcinoma showed positive staininng in central portion of tumor lobules on 4 cases. The low expression of p27 staining was observed well-differentiated squamous cell carcinoma except dyskeratotic cells and horn pearls, but poory-differentiated squamous cell carcinoma showed moderate expression in 2 cases and high expression in 2 cases. Conclusion: These result represent that the decresae of p27 might related to tumorigenesis, and p27 expression might reflect the terminal differentiation of tumor cells.

건물 신선 외기 태양열 난방 제어 시스템에 관한 연구

권기린,현명택,강은철 제주대학교 공과대학 첨단기술연구소 2002 尖端技術硏究所論文集 Vol.13 No.2

Unglazed solarwall installed in south wall is to preheat fresh air by solar insolated energy. This system has been proved as cost effective and easy to design and install and even operate. So, advanced countries such as Canada and USA have already developed and applied the solar wall systems. KIER has started to work on design, analysis and control of the solar wall system. The purpose of this paper is to introduce the solar wall fresh air heating control system. The control system logic as well as hardware will be explained. The impacts of solar wall control system on the room temperature have been investigated. The designed control system to keep the room temperature constant between 18℃ to 25℃ could be developed and demonstrated with LabVIEW 6i software and DAQ hardware in KIER.

溫胞散이 卵巢機能에 미치는 影響

李明珍,李京燮,宋炳基 慶熙大學校韓醫科大學韓醫學硏究所 1995 慶熙韓醫大論文集 Vol.18 No.2

Onposan has reputed to be effective for the remedy of sterility. In order to study the effects of Onposan on ovarian function, a series of experiments were conducted on the ovulation and serum LH, FSH, prolactin and estradiol 17-β level in rats and then the following results were obtained. 1. The increase of the dose of Onposan made a significant effect on ovulation induced PMSG. 2. The increase of the dose of Onposan made a significant effect on ovulation that on the induction of ovulation by PMSG and HCG. 3. The level of serum LH of rats induced hyperprolactinemia was significantly increased in the group of increase of the dose of Onposan. 4. The level of serum FSH of rats induced hyperprolactinemia was not significantly increased. 5. The level of serum Prolactin of rats induced hyperprolactinemia was not significantly increased. 6. The Level of serum Estradiol-17β of rats induced hyperprolactinemia was significantly increased.

성격유형에 따른 복식문양 이미지 평가에 관한 연구

남기선,한명숙 服飾文化學會 2004 服飾文化硏究 Vol.12 No.1

The objectives of this study were to investigate the perceptions of Korean female university students for clothing pattern tendency and structural element of clothing pattern image dimension and to find how individual personality type influence the preferred clothing pattern characteristics. For this study, a questionnaire was designed and sent to 600 female university students of Daejeon, Seoul and metropolitan area. The tool used in this study was MBTI(The Myers-Briggs Type Indicator) Form G Korean version and for the analysis of data SPSS 10.0 package were used. 10 representative patterns for this study were floral, dot, stripe, check, animal, abstract & artistic, geometric, vegetable & leaf, paisely, patchwork pattern. The data were analyzed by factor analysis, arithmetic mean, One-Way ANOVA, χ^(2)-test. The major findings were as follows: Clothing pattern image dimension perceived by Korean female university students for 10 representative patterns were basic form, deluxe, specialty, and cultral dimension. Among them, basic form and deluxe dimension were major dimensions. In basic form dimension, dot pattern score was high indicating female students perceive it as light, comfortable, clean, cool and simple pattern image. In deluxe dimension, floral pattern scored high and in specialty dimension, abstract and artistic pattern scored high among other pattern image. In cultural dimension, geometric pattern and check pattern scored high. Based on other detailed analysis results, It is concluded that the personality type greatly influence clothing pattern evaluation. For example, in case of color combination of patchwork pattern, there was a difference in color preference depend on a personality type such as sensing(S) or intuition(N). Therefore, sensing personality type preferred adjacent color combination than contrast color combination. Detailed marketing strategy is necessary in planning textile design of merchandise plan.