대장균의 전사조절 유전자 nlp의 분자기구 해석

최용락,정수열,정영기,정정한 동의대학교 기초과학연구소 1994 基礎科學硏究論文集 Vol.4 No.1

An nlp (Ner like protein) gene from E. coli was previously cloned and sequenced. Here was show that expression of the sugar metabolism related genes, lacZ, malQ, and malP, increased 2.5- to 8.3-fold in the presence of a plasmid containing the nlp gene. This suggested that the nlp gene could induce maltose- and lactose-metabolism coordinately with crp*1 in the absence of cAMP. Using the nlp-lacZ fusion gene, it was possible to show the promoter of nlp was active in vivo. The overexpressed nlp gene product, a polypeptide of 10,000 daltons, was confirmed by SDS-polyacrylamide gel electrophoresis. The band shift assay revealed that the partially purified Nlp protein bound a specific DNA of the regulatory region of the nlp gene.

以功能語法構建的應用文教材對提高非華語學生功 能字詞運用的成效

Mark Shiu Kee SHUM,Wai Ip LAM,Chung Pui TAI,Paisley Tsz Mei CHEUNG,Jo Jo Wan Shan YUNG,Jessica Man Yi YOUNG 단국대학교 한문교육연구소 2020 International Han-character Education Research Vol.2 No.1

홍콩의 비중국어권 학습자의 실용문 학습과 상황에 맞는 정확한 표현 능력의 제고를 위해 홍콩대학 Dr. Mark Shum Shiu Kee (岑紹基) 연구팀은 교육국의 연구비 지원을 받아 2016~ 2017년 비중국어권 학습자용 실용문 교재를 개발하였다. 본 논문은 기능 어법 이론을 바탕으로 비중국어권 학습자용 실용문 교재를 어떻게 설계했는지를 밝히고, 본 교재가 비중국어권 학습자의 기능어 운용 능력 향상에 끼친 영향에 대해 고찰하였다. 또한 연구팀이 개발한 실용문 교재가 비중국어권 학습자의 기능어 운용 능력 향상에 어떠한 성과를 가져왔는지를 밝히는 것을 본 논문의 주목적으로 삼았다. 연구팀은 체계 기능 언어학 이론을 바탕으로 비중국어권 학습자용 실용문 교재를 개발하였으며, 실용문의 종류에 따라 달라지는 의사전달 기능, 도식 구조, 어법 특징을 고려하여 읽기 예문의 주제를 선정하였다. 또한, 비중국어권 학습자가 기능어와 구문 활용에서 갖는 어려움을 실용문 종류에 따라 나누어 분석하였다. 아울러, 「文類功能(문체기능)」 이론 및 「閱讀促進學習(읽기촉진학습)」 교수법을 참고로 읽기 쓰기 통합형 쌍방향 연습법을 개발하여 학습자가 모범 예문을 분석, 해체 및 재구조화를 할 수 있게 하고, 더 나아가 학습자가 스스로 실용문 쓰기를 할 수 있도록 도왔다. 이외에도, 교육 일선에서 지도하고 있는 중국어 교사들을 초청하여 시범적으로 교수법을 시행하고 이를 통해 본 교재의 실제 효과를 검증해 보았다. 학습자가 작성한 실용문을 교수법 시행 전후로 비교 분석하고 교사와 학습자 인터뷰도 함께 진행하여, 본 실용문 교재가 비중국어권 학습자의 기능어 운용과 실용문 쓰기에 끼친 효과를 평가하였다. 아울러, 본 연구 결과를 바탕으로 비중국어권 학습자 실용문 교육을 위한 실행 가능한 몇 가지 방안을 제시하였다. In order to enhance Hong Kong non-Chinese speaking (NCS) students’ ability to learn practical writing and presenting ideas appropriately in Chinese, Dr. Mark Shum Shiu Kee’s research team received funding from the Hong Kong Education Bureau to develop Chinese practical writing teaching materials for NCS students in 2016/17. This paper describes how the researchers develop these teaching materials by adopting Functional Grammar Theory, and investigates the effectiveness of the teaching material package at equipping the NCS students’ with ability in using function words. This paper aims at exploring how effective the newly developed practical writing teaching material package at enhancing NCS students’ ability to use function words is. With reference to the Systemic Functional Linguistic (SFL) Theory, Chinese practical writing teaching material package suitable for NCS students was developed. The package reading materials were developed by looking into the communicative function, schematic structure and metalanguage of different genres. Besides, NCS students’ difficulty in using function words and expressions of different genres were also analyzed. By referring to “Genre Function” theory and “Reading to Learn” pedagogy, interactive exercises that combine reading and writing were designed. These suggested exercises guide students to learn in a process, from deconstruction of the sample texts to joint construction and individual construction. Apart from the above, frontline Chinese language teachers were invited to conduct tryout sessions to test the effectiveness of the teaching material package. The analysis of students’ writings in pre-tests and post-tests, together with the research data from teachers and students interviews, provide a comprehensive evaluation on the effectiveness of the practical writing teaching material package. Feasible suggestions for enhancing NCS students’ ability, particularly in using function words and constructing practical writing, are provided for teaching NCS students to construct practical writing.

대장균의 전사조절 유전자 nlp의 분자기구 해석

최용락,정수열,정영기,정정한 東亞大學校附設遺傳工學硏究所 1994 遺傳工學硏究 Vol.- No.1

대장균의 nlp(Ner like protein) 유전자를 클로닝하여 구조해석을 한 결과 전사조절 단백질인 Ner와는 61%의 높은 상동성을 가지고 있음을 이미 보고한 바 있다. 본 연구는 당 대사 관련 유전자의 발현 조절을 보고자 nlp 유전자를 도입시켜 tac promoter에 의해 대량 발현시킴으로서 lactose대사 관련 유전자 lacZ와 maltose 대사 관련 유전자인 malQ, malP의 유전자 발현이 2.5배에서 최고 8.3배 정도까지 증가됨을 확인하였다. 이는 nlp유전자가 cAMP비존재하에 crp*1와 상호작용하여 maltose및 lactose대사를 촉진시킴을 시사한다. nlp-lacZ 융합 유전자 산물을 Immunoblotting하여 분석한 결과 nlp의 promoter가 in vivo 상태에서 발현되어짐을 확인하였다. tac promoter와 IPTG에 의하여 nlp유전자 산물을 대량 발현시킨 결과 약 10,000Da의 산물을 SDS-poly-acrylamide gel 전기영동으로서 확인하였으며, 부분 정제된 Nlp단백질을 조절 영역의 DNA단편에 결합함으로서 전사조절에 관여하는 것으로 사료되어졌다. An nlp (Ner like protein) gene from E. coli was previously cloned and sequenced. Here we show that expression of the sugar metabolism related genes, lacZ, malQ and malP, increased 2.5- to 8.3-fold in the presence of a plasmid containing the nlp gene. This suggested that the nlp gene could induce maltose-and lactose-metabolism coordinately with crp*1 in the absence of CAMP. Using the nlp-lacZ fusion gene, it was possible to show the promoter of nlp was active in vivo. The overexpressed nlp gene product, a polypeptide of 10,000 daltons, was confirmed by SDS-polyacrylamide gel electrophoresis. The band shift assay revealed that the partially purified NIp protein bound a specific DNA of the regulatory region of the nlp gene.

「財務諸表의 目的에 관한 報告書」의 分析的 考察

鄭基英 啓明大學校 産業經營硏究所 1978 經營經濟 Vol.9 No.1

Two years after the appointment of the Study Group on the Objectives of Financial Statements, the Study Group producted the Report under the title of Objectives of Financial Statements. Of course, this study was not the first effort by a professional body to deal with the subject of financial statement objectives. But the difference of this study from the other previous inquiries may be largely due to matters in approach and method. The creation of a functional framework of which accounting standards can be based has been a continuing aim of this study. Deducing twelve objectives and eight qualitative characteristics of reporting, the Study Group concluded that the objectives developed in the Report could be looked upon as attainable in stages within a reasonable time and insisted that selecting the appropriate course of action for gaining acceptance of those objectives is not within the purview of the Study Group. However, the Study Group urged that its conclusions be considered as an initial step in developing objctives important for the ongoing refinement and improvement of accounting standards and practices. All the objectives are presented in the Report as apparent equals. Though they are showed in that way the objectives differ significantly from each other in aim or emphasis. Some are only means of achieving other objectives. The hierachical structure gives cohesion to ten of the objectives, and the ability to see the structure is essential to interpret several objectives and to see their relation to more basic or general objectives. Though there are other viewpoints in the hierachical structure, the first ten objectives(objective No.1-10) in the Report from a structure with five levels. Two objectives(objective No.11-12) in the Report do not fit into the hierarchical structure, though they are not necessarily extraneous to Study Group's task. Significant parts of the Report are capable of present adoption, either explicitly to guide current practice or as goals to be attained in the future. They are objective No. 1,3,4,6,7,8,9,10, and qualitative characteristics except consitstency. Other parts of the Report can not be adopted because they need additional study. In that group are objective No.2,5,11,12 and consitency, a part of financial reporting. The Study Group is generally accepted successful in doing their role to search objectives of financial statements.

입원 남성 및 여성 알코올리즘 환자의 인구학적, 임상적 및 인격적 특성비교

정원후,김성부,장기용,손진욱,박철수 大韓神經精神醫學會 1997 신경정신의학 Vol.36 No.4

저자들은 1996년 5월 1일부터 1996년 7월 15일까지 부산·경남·대구 소재의 대형정신병원과 종합병원에 입원해 있는 여성 알코올리즘을 대상으로 하고, 또한 같은 기간에 국립부곡정신병원에 입원한 남성 알코올리즘을 비교군으로 해서 사회인구학적 특성, 음주양상, 음주와 관련된 문제점, 성격 및 정신병리등을 비교하여 남녀 알코올리즘에서의 원인과 임상적 특성에서의 차이점을 알고자해서 본 연구를 하게 되었다. 저자들이 작성한 반구조화된 설문지, MAST(Michigan alcoholism screening test). Zung의 SAS(Self-rating anxiety scale)과 SDS(Self-rating depression scale). MMPI(Minnesota Multiphasic Personality Inventory), SCL-90-R(Symptom checklist-90-revision) 및 EPQ(Eysenck personality questionnaire)등의 검사도구를 이용하였고, 그 결과를 독립표본 t-test(independent-samples t-test)와 χ²-test로 검정하여 다음과 같은 결론을 얻었다. 1) 여성 알코올리즘에서 남성 알코올리즘보다 더 낮은 교육 수준을 보였으며 초등학교수준 이하가 52.4%를 차지하였다. 반면에 남성 알코올리즘에서는 중학교·고등학교 수준이 76.7%였다.(p〈0.01). 2) 여성 알코올리즘에서 형제들 중에서 더 낮은 서열을 보였으며 세째딸이 1/3로 가장 많았고 다음이 외동딸로써 23.8%를 차지하였다. 반면 남성 알코올리즘에서는 장남이 46.7%였다.(p〈0.01). 3) 여성 알코올리즘이 사회경제적 수준에서 더 낮았다(p〈0,01). 4) 여성 알코올리즘의 음주시작동기는 대인관계를 위한 사교목적, 심리적 갈등이나 다른 정신적인 문제, 가족들과의 갈등 등이 비슷하게 많았다. 그러나 남성 알코올리즘에서는 대인관계를 위한 사교목적으로 음주하는 경우가 절대적으로 많았다.(p〈0.05). 5) 처음으로 음주를 시작한 연령은 여성 알코올리즘보다는 남성 알코올리즘에서 유의하게 더 어린 연령에서 시작하였다.(p〈0.001). 6) 1일 평균 음주량은 여성 알코올리즘에서 유의하게 더 적었다.(p〈0.01). 7) 금단증상경험, 알코올성 신체장애 및 음주로 인한 법적 처벌경험도 남성 알코올리즘보다는 여성 알코올리즘에서 유의하게 더 낮았다.(p〈0.05). 8) SCL-90-R에서 PSY척도에서 유의하게 여성 알코올리즘군에서 더 높았고, EPQ에서는 L척도에서 유의하게 여성 알코올리즘이 더 높았다(p〈0.05). 결론적으로 본 연구는 심리적 요인과 사회적 요인이 남녀 알코올리즘간에서 많은 차이가 있는 것을 알 수 있었으며, 여성 알코올리즘의 원인에는 심리적 또는 사회인구학적 요인이 더 밀접하게 연관이 되어 있음을 알 수 있었다. Objective : This study was designed to compare the characteristics of demographic data, clinical features, and personality in male and female alcoholics and to identify the clinically meaningful differences in the etiologies and the clinical features between the two groups. Methods : The study group was composed of 30 males and 21 females who were recruited into the impatient treatment. The researchers used self-reporting questionnaire composed of demographic data and drinking patterns, Michigan alcoholism screening test(MAST), self-rating anxiety scale(SAS) and self-rating depression scale(SDS) of Zung, Minnesota Multiphasic Personality Inventory(MMPI), symptom Checklist-90-revision(SCL-90-R), and Eysenck personality questionnaire(EPQ). The differences between the two groups were tested by chi-square test and independent-sample t-test. Result : 1) Education level of the female alcoholics was significantly less than that of male alcoholics(p〈0.05) and the birth order the female alcoholics was significantly higher than that of male alcoholics(p〈0.05). 2) The level of socioeconomic status of female alcoholics was significantly lower than that of male alcoholics(p〈0.01). 3) The initial motivations of female alcoholics were mainly due to social, psychological, and intrafamilial problems while those of male alcoholics were due to social problems. There were significant difference between the two groups(p〈0.05). 4) The onset of initial drinking in female alcoholics was significantly earlier than that of male alcoholics(p〈0.001). 5) The average amount of daily drinking in female alcoholics was significantly less than that of male alcoholics(p〈0.01). 6) Withdrawal symptoms, physical disorder, and social or legal problems induced by alcohol drinking in female alcoholics were significantly less than those of male alcoholics(p〈0.05). 7) The mean scores of PSY scale of SCL90R and of L scale of EPQ in female alcoholics were significantly higher than those of male alcoholics(p〈0.05). Conclusion : Demographic and psychosocial factors differed to a significant degree between male and female alcoholism. We suggest that these factors are more important in female alcoholism than in male one in the etiologies and the clinical features. These differences are due to more complex factors rather than simple ones, thus the further evaluations are needed to elucidate the differences in the etiologies between male and female alcoholism.

한글 Logo 개발 및 그래픽 인터페이스에 관한 연구

鄭洪,金鎭相,朴起賢 계명대학교 산업기술연구소 1985 産業技術硏究所 論文報告集 Vol.7 No.-

This paper reports on aspects in the design and implementation of Hanguel Logo programing language which reflects a model in the design and implementation of Hanguel programing language. This article contains an in-depth analysis of Logo interpreter and many problem involved in the implementation procedure of Hanguel Logo, and some methods on resolving them. Design and implementation of a graphic interface for Logo's turtle geometry are also presented.

Capillaria hepatica 感染白鼠에 있어서 蟲卵抗原을 이용한 免疫診斷에 관한 比較硏究

鄭然郁,嚴基善,林漢鍾 고려대학교 의과대학 1985 고려대 의대 잡지 Vol.22 No.3

Comparative studies were performed for Capillaric hepatica antibodies with following immunodiagnostic assays : circumoval precipitation reaction(COP) with lyophilized eggs as antigen, indirect fluorescent antibody test (IFAT) with sectioned egg antigen, enzyme-linked immunosorbent assay(ELISA) with soluble egg antigen and counterimmunoelectrophoresis (CIEP) with soluble egg antigen. In the first part of this study it was found that every type of egg antigens used were effective and showed almost equal sensitivities with proper immunodiagnostic methods. Additionally, antibodies to C. hepatica were detectable only during short period after experimental infection of embryonated eggs. Sera from 40 white rats infected with 2,000 eggs orally were collected randomly between three and nine weeks after infection and the infection with C. hepatica was identified by necropsy. COP, ELISA & CIEP had diagnostic sensitivities of 85.0% and IFAT, 82.5 %. Sera from 5 white rats infected with 2,000 eggs were collected periodically to observe the changing patterns of antibody titers by IFAT and ELISA, which showed that high titers were detected at weeks 3 and 5, then gradually declined through weeks 9 until to negatively converted at weeks 13. The immunodiagnostic method of choice was recommended as ELISA since the antibodies were detectable with lowest protein contents(1.25 ㎍/ml) of antigen and highest dilution of sera (1 : 3,000), even though the equal sensitivity with other methods by different antigen and antibody conditions.

經濟환경의 변화와 會計관련 規制 : 기업회계기준 및 관련법률의 개정내용을 중심으로

鄭基英 啓明大學校 産業經營硏究所 1997 會計情報리뷰 Vol.- No.3

WTO 체제의 완성이 가까워 옴에 따라 각종 규제가 자유경쟁체제를 바탕으로 풀리게 되고 우리나라의 OECD가입으로 국제화의 바람은 멈추지 않고 있다. 이에 따라 자본시장의 자율화, 회계산업의 개방은 가속적으로 추진되고 있고 따라서 1996년 두차례에 걸쳐 기업회계 기준이 개정된 바 있다. 1996년과 1997년에 걸쳐 회계정보이용자들이 필요로 하는 정보의 생산과 유통을 광범위 하게 규정하고 있는 중심적 법규인 증권거래법을 위시하여, 상행위와 주식회사를 중심으로 私的 자치기능을 위한 제도적장치를 마련함과 아울러 투자자와 채권자를 보호할 목적으로 商法이 대폭개정되었다. 또한 주식회사의 외부감사에 관한 법률도 상법상의 감사와 외부감사와의 관계 및 감사인선임방법과 회계법인 평가등을 새로이 규정함으로써 경영의 투명성 및 경쟁력 강화를 도모하고 회계시장 개방에 대비케 하였다. 공인회계사법의 개정으로 손해배상책임제도를 강화하고 회계법인의 사원을 유한책임을 지도록 하며, 한국공인회계사회에 각종의 권한과 책임을 부여하여 국제화, 자율화의 틀에 맞도록 하였다. 이러한 회계관련 주요 법률 및 기준의 개정은 환경의 변화에 따른 것으로 이러한 규정들의 지속시간이 짧아질 것이고 내용도 다양성, 일시성, 새로움을 추구하는 방향으로 개정되어 나갈 것이다. 본 연구는 관련규제의 개정내용을 요약하여 회계규제 체계를 이해하는데 도움을 주며 앞으로의 분석적 연구에 터를 닦을 목적으로 수행된 것이며 문헌조사를 위주로한 기술적 연구이다.

公表된 利益이 서울증권시장에 미치는 영향에 관한 硏究

鄭基英 啓明大學校 産業經營硏究所 1988 經營經濟 Vol.21 No.1

There have been revolutionary research changes in the accounting academia. One of them is an empirical research using accounting data along with recently developed financial theories(or models). Powerful helps from those financial theories make accounting academia and accountants interpret their study results and/or practices with a scientific manner to their interested parties. Objcetives of this study are to introduce a very fundamental model developed by W.H.Beaver and to test a validity of that model in the Seoul Securities Exchange through 10 years range(1976-1985). From a parsimonious random sampling. 61 sample firms were selected. Stock price changes and transaction volume changes were examined through 17 weeks(8 weeks before accounting data disclosure week and 8 weeks after that). Conclusions of this study are as follows: 1) Accounting income number has, although it is not perfect, a kind ofinformation content(thus, it can be explained that Seoul Securities Exchange as a whole perceive accounting income number as one of information resources). 2) Accounting income number has been perceived, no information content to the individual investors(this result was analyzed from the examination of the transaction volume changes). However, this study has several limits, such as small number of sample firms, size problems, and no consideration of the Korean Government's intervention to the Seoul Securities Exchange, etc. If those limits were cured in some way, the results might have changed.

식물 추출물에서 β-Amyloid Peptide 응집 억제제 검출

정민아,최기오,안봉환 全南大醫大 2001 전남의대학술지 Vol.37 No.4