Effects of Ubiquitin-conjugating Enzyme 2C on Invasion, Proliferation and Cell Cycling of Lung Cancer Cells

Tang, Xiao-Kui,Wang, Ke-Jian,Tang, Yu-Kui,Chen, Li Asian Pacific Journal of Cancer Prevention 2014 Asian Pacific journal of cancer prevention Vol.15 No.7

The aims of this study were to investigate the influence of ubiquitin- conjugating enzyme E2C (UBE2C) on biological behavior of lung cancer cells. Using MTT, flow cytometry and invasion assays, we detected UBE2C expression and evaluated its biological properties in these cells, including effects on proliferation, the cell cycle profile and invasive capability. Compared with control cells, the UBE2C transfected cells demonstrated increased cellular proliferation (p<0.05). UBE2C transfected cells also had a lower percentage in G1 phase and a higher percentage in S phase (p<0.05). Importantly, the UBE2C transfected cells had a notable enhancement of cell numbers penetrating the basement membrane compared with the control group (p<0.05). Ectopic up-regulation UBE2C promoted the growth of lung cancer cells in vivo. Furthermore, we found UBE2C increased the expression of cyclin D1 and MMP-2. These results show UBE2C may represent a potential therapeutic target for lung cancer.

Equivalent static wind loads analysis of tall television towers considering terrain factors of hilltops based on force measurement experiment

Shi-Tang Ke,Hao Wang,Yao-Jun Ge,Lin Zhao,Shuyang Cao 국제구조공학회 2017 Structural Engineering and Mechanics, An Int'l Jou Vol.63 No.4

Wind field in mountainous regions demonstrates unique distribution characteristic as compared with the wind field of the flat area, wind load and wind effect are the key considerations in structural design of television towers situated in mountainous regions. The television tower to be constructed is located at the top of Xiushan Mountain in Nanjing, China. In order to investigate the impact of terrain factors of hilltops on wind loads, firstly a wind tunnel test was performed for the mountainous area within 800m from the television tower. Then the tower basal forces such as bending moments and shear strength were obtained based on high frequency force balance (HFFB) test. Based on the experiments, the improved method for determining the load combinations was applied to extract the response distribution patterns of foundation internal force and peak acceleration of the tower top, then the equivalent static wind loads were computed under different wind angles, load conditions and equivalent goals. The impact of terrain factors, damping ratio and equivalent goals on the wind load distribution of a television tower was discussed. Finally the equivalent static wind loads of the television tower under the 5 most adverse wind angles and 5 most adverse load conditions were computed. The experimental method, computations and research findings provide important references for the anti-wind design of high-rise structure built on hilltops.

A study on the working mechanism of internal pressure of super-large cooling towers based on two-way coupling between wind and rain

Shi-Tang Ke,Wenlin Yu,Yao-Jun Ge 국제구조공학회 2019 Structural Engineering and Mechanics, An Int'l Jou Vol.70 No.4

In the current code design, the use of a uniform internal pressure coefficient of cooling towers as internal suction cannot reflect the 3D characteristics of flow field inside the tower body with different ventilation rate of shutters. Moreover, extreme weather such as heavy rain also has a direct impact on aerodynamic force on the internal surface and changes the turbulence effect of pulsating wind. In this study, the world\'s tallest cooling tower under construction, which stands 210m, is taken as the research object. The algorithm for two-way coupling between wind and rain is adopted. Simulation of wind field and raindrops is performed iteratively using continuous phase and discrete phase models, respectively, under the general principles of computational fluid dynamics (CFD). Firstly, the rule of influence of 9 combinations of wind speed and rainfall intensity on the volume of wind-driven rain, additional action force of raindrops and equivalent internal pressure coefficient of the tower body is analyzed. The combination of wind velocity and rainfall intensity that is most unfavorable to the cooling tower in terms of distribution of internal pressure coefficient is identified. On this basis, the wind/rain loads, distribution of aerodynamic force and working mechanism of internal pressures of the cooling tower under the most unfavorable working condition are compared between the four ventilation rates of shutters (0%, 15%, 30% and 100%). The results show that the amount of raindrops captured by the internal surface of the tower decreases as the wind velocity increases, and increases along with the rainfall intensity and ventilation rate of the shutters. The maximum value of rain-induced pressure coefficient is 0.013. The research findings lay the basis for determining the precise values of internal surface loads of cooling tower under extreme weather conditions.

Wind-sand coupling movement induced by strong typhoon and its influences on aerodynamic force distribution of the wind turbine

Shi-Tang Ke,Yifan Dong,Rongkuan Zhu,Tongguang Wang 한국풍공학회 2020 Wind and Structures, An International Journal (WAS Vol.30 No.4

The strong turbulence characteristic of typhoon not only will significantly change flow field characteristics surrounding the large-scale wind turbine and aerodynamic force distribution on surface, but also may cause morphological evolution of coast dune and thereby form sand storms. A 5MW horizontal-axis wind turbine in a wind power plant of southeastern coastal areas in China was chosen to investigate the distribution law of additional loads caused by wind-sand coupling movement of coast dune at landing of strong typhoons. Firstly, a mesoscale Weather Research and Forecasting (WRF) mode was introduced in for high spatial resolution simulation of typhoon “Megi”. Wind speed profile on the boundary layer of typhoon was gained through fitting based on nonlinear least squares and then it was integrated into the user-defined function (UDF) as an entry condition of small-scaled CFD numerical simulation. On this basis, a synchronous iterative modeling of wind field and sand particle combination was carried out by using a continuous phase and discrete phase. Influencing laws of typhoon and normal wind on moving characteristics of sand particles, equivalent pressure distribution mode of structural surface and characteristics of lift resistance coefficient were compared. Results demonstrated that: Compared with normal wind, mesoscale typhoon intensifies the 3D aerodynamic distribution mode on structural surface of wind turbine significantly. Different from wind loads, sand loads mainly impact on 30° ranges at two sides of the lower windward region on the tower. The ratio between sand loads and wind load reaches 3.937% and the maximum sand pressure coefficient is 0.09. The coupling impact effect of strong typhoon and large sand particles is more significant, in which the resistance coefficient of tower is increased by 9.80% to the maximum extent. The maximum resistance coefficient in typhoon field is 13.79% higher than that in the normal wind field.

United Electromagnetic Characteristics and Online Monitoring Method of Static Air-gap Eccentricity of Turbo-Generator

Tang, Gui-Ji,Ke, Meng-Qiang,He, Yu-Ling,Wang, Fa-Lin The Korean Institute of Electrical Engineers 2016 Journal of Electrical Engineering & Technology Vol.11 No.6

The purpose of this paper is to investigate the united Electromagnetic characteristics for the effective monitoring on the static air-gap eccentricity (SAGE) of turbo-generator. Different from other studies, this paper not only studies on the unbalanced magnetic pull (UMP) and the vibration characteristics of the stator and the rotor, but also investigates the harmonic features of the magnetic flux density and the circulating current inside the parallel branches (CCPB). The theoretical calculation, together with the finite-element-method (FEM) simulation and the experiment verification, is taken for a SDF-9 type non-salient generator. It is shown that, when SAGE occurs, apparent double-frequency UMP and vibrations will be produced both on the stator and the rotor, while the CCPB will have an obvious increment at the $1^{st}$ harmonic component. In addition, the amplitude of the magnetic flux density will be of cosine distribution in the circumferential position of the air-gap, while in normal condition it is a constant. Moreover, the pass-band amplitude, together with the $1^{st}$ harmonic of the magnetic flux density, will be enlarged as well. These united electromagnetic characteristics can be used as the diagnosis and monitoring criterion for SAGE.

Effects of Turbulent Dispersion on Water Droplet Impingement Based on Statistics Method

Ke Peng,Tang Xing,Lu Yi,Yang Hui Yun 한국항공우주학회 2018 International Journal of Aeronautical and Space Sc Vol.19 No.2

Researchonthewaterdropletmotionandimpactonsurfaceisthebasisofaircrafticingpredictionanddesignoficeprotection system. First, a new computational method based on statistical theory was developed in the Euler–Lagrange framework to calculatethelocalwaterimpingementcoefficientonthesurface,whichcouldbeusedtostudytheeffectofturbulentdispersion onthewaterdropletmotionandimpingementcharacteristics.Second,basedontheRANSmodelfortheairflowfield,taking engineconesurfacewithorwithoutslotasexamples,theinfluenceofturbulentdispersiononthewaterimpingementcoefficient on the surface was comparatively analyzed with the discrete random walk model. For the cone without film slot, turbulence causes a little impact on water droplet impingement. However, for the slotted cone, water droplet collection calculation must consider the effect of turbulence due to the stronger turbulence induced by the jet air. Further studies show that the effect is enhanced with the decrease of water droplet diameter. Finally, discussion about turbulent effects was proposed. This researchabout theinfluence ofturbulentdispersionwillbehelpfultoimprovetheaccuracy ofthewaterdropletimpingement calculation for some complex geometries.

Cutting force and machine kinematics constrained cutter location planning for five-axis flank milling of ruled surfaces

Ke Xu,Jiarui Wang,Chih-Hsing Chu,Kai Tang 한국CDE학회 2017 Journal of computational design and engineering Vol.4 No.3

Five-axis flank milling has been commonly used in the manufacturing of complex workpieces because of its greater productivity than that of three-axis or five-axis end milling. The advantage of this milling operation largely depends on effective cutter location planning. The finished surface sometimes suffers from large geometrical errors induced by improper tool positioning, due to the non-developability of most ruled surfaces in industrial applications. In addition, a slender flank-milling cutter may be deflected when subjected to large cutting forces during the machining process, further degrading the surface quality or even breaking the cutter. This paper proposes a novel tool path planning scheme to address those problems. A simple but effective algorithm is developed to adaptively allocate a series of cutter locations over the design surface with each one being confined within an angular rotation range. The allocation result satisfies a given constraint of geometrical errors on the finished surface, which consists of the tool positioning errors at each cutter location and the sweeping errors between consecutive ones. In addition, a feed rate scheduling algorithm is proposed to maximize the machining efficiency subject to the cutting force constraint and the kinematical constraints of a specific machine configuration. Simulation and experimental tests are conducted to validate the effectiveness of the proposed algorithms. Both the machining efficiency and finish surface quality are greatly improved compared with conventional cutter locations.

Room-temperature tensile strength and thermal shock behavior of spark plasma sintered W-K-TiC alloys

Ke Shi,Bo Huang,Bo He,Ye Xiao,Xiaoliang Yang,Youyun Lian,Xiang Liu,Jun Tang 한국원자력학회 2019 Nuclear Engineering and Technology Vol.51 No.1

W-K-TiC alloys with different titanium carbide concentrations (0.05, 0.1, 0.25, 0.5, 1, 2) wt.% werefabricated through Mechanical Alloying and Spark Plasma Sintering. The effects of the addition of nanoscaledTiC particles on the relative density, Vickers micro-hardness, microstructure, crystal information,thermal shock resistance, and tensile strength were investigated. It is revealed that the doped TiC nanoparticleslocated at the grain boundaries. The relative density and Vickers micro-hardness of W-K-TiCalloys was enhanced with TiC addition and the highest Vickers micro-hardness is 731.55. As the TiCaddition increased from 0.05 to 2 wt%, the room-temperature tensile strength raised from 141 to353 MPa. The grain size of the W-K-TiC alloys decreased sharply from 2.56 mm to 330 nm with theenhanced TiC doping. The resistance to thermal shock damage of W-K-TiC alloys was improved slightlywith the increased TiC addition

Novel Compact Bandpass Filter Based on Folded Half Mode Substrate Integrated Waveguide Cavities

Ke Gong,Wei Hong,Jixin Chen,Hongjun Tang,Debin Hou,Yan Zhang 한국전자파학회JEES 2010 Journal of Electromagnetic Engineering and Science Vol.10 No.3

This paper proposed a novel compact bandpass filter with folded half mode substrate integrated waveguide (FHMSIW) cavities using two-layer printed circuit board(PCB) process. The area of the FHMSIW filter is reduced by nearly 50 % and 75 % compared with half mode substrate integrated waveguide(HMSIW) filter and substrate integrated waveguide(SIW) filter, respectively. A four-pole Chebyshev FHMSIW bandpass filter at C-band has been designed, simulated and fabricated. Measured results are presented and found to agree with the full-wave simulated results by using Ansoft HFSS. The filter shows good performance and compact size.

Air and moisture stable robust bio-polymeric palladium catalyzed C-C bond formation and its application to the synthesis of fungicidal

Ke Chen,Guangzu He,Qiong Tang 한국공업화학회 2023 Journal of Industrial and Engineering Chemistry Vol.124 No.-

Cellulose supported bio-heterogeneous polymeric Pd(II) catalyst, stable to air and moisture, was designedand characterized with different spectroscopic techniques including FESEM, TEM, XPS, XRD, ICP-AES andEDX. The Pd(II) catalyst showed high catalytic performance (0.2 to 0.0009 mol%) to the Suzuki-MiyauraCAC cross-coupling reaction of aromatic halides and aryl diazonium salts with a range of organoboronicacids to produce the respective biaryl product in up to 97% yield. Biologically active Boscalid, a fungicidethat effectively inhibits the growth of fungal pathogens like ascomycetes on fruits and plants, could alsobe constructed through using this catalyst. Additionally, it was possible to recover the Pd(II) catalyst fromthe reaction medium and reused it repeatedly without significantly degrading its catalytic efficiency.