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Shim, Doo-Bo,Park, Eun-Sil,Sim, Gyu-Jin,Lee, Ji-Young,Kang, Ju-Hwan,Yoo, Hyun-Joo,Choi, Yeon-Jin,Lee, Young-Mee,Lee, Sang-Yeol,Kim, Min-Gab,Kang, Da-Won,Jung, Eun-Jung,Kang, Kee-Ryeon The Korean Society for Applied Biological Chemistr 2011 Applied Biological Chemistry (Appl Biol Chem) Vol.54 No.4
Eukaryotic translation initiation factor 5A (eIF5A) is the only hypusine-containing protein, which is formed by deoxyhypusine synthase (DHS) and deoxyhypusine hydroxylase (DOHH). DOHH is a novel metalloenzyme with HEAT [named for human huntingtin (H), elongation factor 3 (E), a subunit of protein phosphatase 2A (A), and the target of rapamycin (T)]-repeat motifs. Inspite of much progress in determining the roles of iron-containing DOHH holoenzyme as an eIF5A hydroxylase, little is known about iron-free apoenzyme of DOHH under certain stress conditions. For this purpose, we compared cell growth in two yeast strains subjected to oxidative damage. Thus, the existence of more viable cells in the Saccharomyces cerevisiae BY4743 (parental yeast) strain than in the DOHH- strain after $H_2O_2$ treatment indicates the importance of DOHH in protecting yeast cells against oxidative stress. To identify endogenous target proteins influenced by DOHH under oxidative damage, proteomic analysis was applied to the two yeast strains. Of these proteins, the oxidized form of peroxiredoxin I (PrxI) was concomitantly up-regulated in both strains under $H_2O_2$ treatment. Two-dimensional electrophoresis (DE) followed by immunoblot analysis shows that the recovery of the oxidized PrxI to the reduced enzyme under $H_2O_2$ treatment was found to be much slower in the DOHH- strain than in the parental strain. Based on the results, we discovered a possible interaction between DOHH and PrxI by immunoprecipitation/immunoblotting in yeast under oxidative stress. Taken together, these results suggest that DOHH might be a candidate protein for protection of yeast cells against oxidative stress in conjunction with PrxI.
Kang-Moon Song,Doo Yong Chung,Min Ji Choi,Kalyan Ghatak,Nguyen Nhat Minh,Anita Limanjaya,Mi-Hye Kwon,옥지연,Guo Nan Yin,Dae-Kee Kim,Ji-Kan Ryu,Jun-Kyu Suh 대한남성과학회 2020 The World Journal of Men's Health Vol.38 No.4
Purpose: To examine the therapeutic effect of Vactosertib, a small molecule inhibitor of transforming growth factor-β (TGF-β) type I receptor (activin receptor-like kinase-5, ALK5), in an experimental model of Peyronie’s disease (PD) and determining anti-fibrotic mechanisms of Vactosertib in primary fibroblasts derived from human PD plaques. Materials and Methods: Male rats were randomly divided into three groups (n=6 per group); control rats without treatment; PD rats receiving vehicle; and PD rats receiving Vactosertib (10 mg/kg). PD-like plaques were induced by administering 100 μL of each of human fibrin and thrombin solutions into the tunica albuginea on days 0 and 5. Vactosertib was given orally five times a week for 2 weeks. On day 30, we performed electrical stimulation of the cavernous nerve to measure erectile function, and the penis was obtained for histological examination. Fibroblasts isolated from human PD plaques were used to determine the anti-fibrotic effects of Vactosertib in vitro. Results: Vactosertib induced significant regression of fibrotic plaques in PD rats in vivo through reduced infiltration of inflammatory cells and reduced expression of phospho-Smad2, which recovered erectile function. Vactosertib also abrogated TGF- β1-induced enhancement of extracellular matrix protein production and hydroxyproline content in PD fibroblasts in vitro by hindering the TGF-β1-induced Smad2/3 phosphorylation and nuclear translocation, and fibroblast-to-myofibroblast transdifferentiation. Conclusions: In view of the critical role of TGF-β and the Smad pathway in the pathogenesis of PD, inhibition of this pathway with an ALK5 inhibitor may represent a novel, targeted therapy for PD.
Cheol-Kee Min,Doo-Hwan Ji,Soon-Cheol Chung,Jin-Kyu Kang,Seunghee Hong,Shun'ichi Doi,Byung-Chan Min 대한인간공학회 2012 대한인간공학회 학술대회논문집 Vol.2012 No.5
In this study, it was observed how the supply of highly concentrated oxygen(40%) could affect HR while performing the addition tasks in a vehicle graphic simulator. The subjects of this study were 17 males in their twenties, and the addition tasks were performed in oxygen concentration(21%, 40%). For the performance of double-digit addition tasks in the straight course of two-lane road, its data was extracted by 30sec section from the start of the task and was analyzed. The test was proceeded in the order of Control (5min), Driving (2min), Driving + Task (1min), and Rest (10min). As a result of analyzing HR, there was a significant difference (p<0.01) between 21% oxygen concentration and 40% oxygen concentration, and HR increased during 21% oxygen concentration. Also, the significant difference between stimuli(Control, Driving, Driving+Task) was recognized, and as a result of the posteriori test, compared with Control, HR increased in Driving+Task, showing a significant difference(p<0.01). That is, the sympathetic nerve of the drivers were activated while performing the addition tasks during driving performance. Especially, when highly concentrated oxygen(40%) was presented, HR reduced. And thus, it was found out that highly concentrated oxygen(40%) had a positive effect on performing addition tasks while driving.