Overexpression of Zoysia ZjCIGR1 gene confers cold stress resistance to zoysiagrass

Yang‑Ji Kim,Dae‑Hwa Yang,Mi‑Young Park,Hyeon‑Jin Sun,Pill‑Soon Song,Hong‑Gyu Kang,Seok‑Cheol Suh,Yong‑Eok Lee,이효연 한국식물생명공학회 2020 Plant biotechnology reports Vol.14 No.1

Zoysia japonica Steud. is a warm-season lawn grass popular in Korea and elsewhere. They are cultivated in many places such as river banks, roadside, and play grounds. However, there still is a disadvantage of frequent mowing, and the grass grows poorly under the chilly conditions. To develop a grass variety that circumvents these drawbacks, we cloned the chitininducible gibberellins-responsive1 gene (CIGR1) from zoysiagrass. The full length of the ZjCIGR1 (Zj; Zoysia japonica Steud.) gene was obtained by 5′/3′ RACE PCR and the phylogenetic tree showed that it belonged to the CIGR1-subgroup in the PAT1-group of GRAS protein family. Expression of the ZjCIGR1 in wild-type plants was confirmed in roots, meristems, leaves, and flowers, especially high in the flowers. The transgenic zoysiagrass was confirmed by PCR using gene-specific primers, phosphinothricin-acetyl-transferase (PAT) strip test, and Southern blot analysis. ZjCIGR1-overexpressing plants acquired tolerance to cold stress displaying morphological phenotypes characteristic of stress resistance. In addition, in the transformants, expression of the ZjCIGR1 as well as cold-regulated (COR) gene was increased compared to the wild-type plants under cold stress condition. These results suggest that ZjCIGR1 gene is an important candidate for regulating cold stress resistance.

The Effect of Cultural Factors on Anther Culture in Spring Wheat (Triticum aestivum)

Kang,Tae-Jin,Yang,Deok-Chun 한국자원식물학회 2003 Plant Resources Vol.6 No.2

Effects of shaking, medium consistency and anther density on polyhaploid production in two wheat cultivars, Pavon and Chris, were studied using a modified 85D12 medium. Pavon produced more calli in shaking and more albino plants tban Chris. However, Chris produced threefold more green plants than Pavon in non-shaking treatment. More calli and green plants were derived from non-shaking treatment than those from shaking treatment. Anthers were cultured on both liquid and semi-solid 85D12 media, using two anther densities, 48 and 96 anthers per plate. Although Pavon generally produced more calli and albino plants than Chris, Chris produced more green plants than Pavon. More green plants were derived from semi-solid medium than those from liquid medium. A factor that may affect plant regeneration from anthers is the length of time on initiation medium. Most of the calli for both genotypes were transferred during the first two time periods. Fertility, as measured by seed set, was determined for all surviving regenerated plants. About 24% of Chris and Pavon anther-derived green plants in the experiment of medium consistency and anther density produced seed.

Days to Germination and Effect of Growth Regulator on Rhizome Growth in Cymbidium goeringii Hybrid

Kang,Tae-Jin,Yang,Deok-Chun 한국자원식물학회 2003 Plant Resources Vol.6 No.2

Germination efficiency, such as days to germination and conditions for the hybrid seeds, was tested after hybrid seeds were sown on the medium. Seeds were germinated from 67 to 126 days after seeding in all cross combinations, and germination condition was different among hybrid combinations. There was big difference on days to germination based on the different media, that is, days to germination in the hyponex medium was shorter than that in the Knudson C medium. Mutants such as MMS and NaN₃ were used to cause mutation. Germination occurred with 0.1 % NaN₃ and MMS. However, even though germination occurred in other treatments (0.01 % and 0.2% of NaN₃ and MMS), brownish phenomenon was intense, or did not proceed and got worse after 4-5 months of seeding. In addition, it was performed to choose appropriate medium for the growth of Cymbidium rhizome whose media adaptability is more different than other orchids. Different concentration of NAA and Kinetin was used. As a result, C. goeringii hybrid showed difference for the concentration of NAA and Kinetin combination.

8년간(1992~1999) 산업안전보건연구원에 의뢰된 직업병 심의 사례 분석

강성규,김규상,김양호,최정근,안연순,진영우,최병순,양정선,김은아,채창호,최용휴,김대성,박정선,정호근 대한산업의학회 2000 대한직업환경의학회지 Vol.12 No.2

Objectives : Pneumoconisis and noise-induced hearing loss (NIHL) have been reported as main occupational diseases by the Special Health Examination. The Industrial Accident Compensation Insurance has reported various work-related diseases, however, these two diseases occupied almost a half of compensated cases. Therefore, it was not well known about the status of occupational diseases other than pneumoconiosis, NIHL, and cardio-cerebrovascular accident (CVA). This study was conducted to analyze claimed cases as an occupational disease, that was requested to the Korea Industrial Safety and Health Agency (KOSHA). Methods : The local office of the Korea Labor Welfare Corporation (KLWC) has asked the KOSHA for confirmation of claimed cases as an occupational disease. We analyzed 379 cases requested from KLWC, the Ministry of Labor, employers, unions and occupational health agencies from 1992 to 1999. Results : Male was 80.7 % of the requested cases. Their mean age was 42 years old and 75.5 % of them were more than 35 years old. The requested cases were increased rapidly from 25 cases in 1992 to 108 cases in 1999 and the accept rate was 50.7 %. The majority of requested cases were respiratory diseases(22.4%), cancers(18.5%), Neuropsychiatric problems(14.5%), and musculoskeletal problems(13.5%). The accept rate was high in reproductive, respiratory, musculoskeletal and digestive disorders and low in neuropsychiatric, renal and otologic problems and occupational cancers. 73.6% of them were caused by chemical agents, especially 28.5 % were by organic solvents. 67% of them were clinically confirmed at university hospitals. A half of the cases were from KyongIn area, even the request came from the whole country. Conclusions : A claim was common in workers whose age was over 35 years old and exposure history was over 10 years. The respiratory diseases and neuropsychiatric disorders were still main problems in occupational health and occupational cancers was increasing even though its accept rate was not high yet.

KMI 라이브러리 개발

梁亨圭,이강호 강남대학교 산학기술연구소 2001 산학기술연구소논문집 Vol.- No.11

Phosphamidon 분해세균의 분리동정 및 생분해능

강양미,허성남,안태석,송홍규 江原大學校 附設 環境硏究所 1999 環境硏究 Vol.16 No.-

토양으로부터 유기인계 살충제인 phosphamidon을 분해하는 세균들을 분리하고 Biolog system을 이용하여 동정하였다. 그람양성 세균들은 모두 Bacillus 속에 속하는 종들이었으며 그람음성 세균들은 토양에서 우점하지 않는 세균들이 많았다. 이들중 phosphamidon 함유배지에서 생장률이 높은 균주들을 선택하여 phosphamidon 분해능을 조사한 결과 Capnocytophaga gingivalis로 동정된 YD-17 균주가 가장 높은 생분해능을 나타내어 1000 ppm의 phosphamidon이 배양 21일 후 9.4%의 잔류량을 보였으며 이는 대조구에 비해 제거율이 52% 증가된 결과였다. 이 때 균주의 생장을 단백질량으로 측정하였는데 분해균주들이 고농도의 phosphamidon에 의해 저해되지 않고 지속적인 생장을 하여 phosphamidon을 탄소원으로 이용하는 생분해가 일어난 것을 확인할 수 있었다. Organphosphorus insecticide phosphamidon-degrading bacteria were isolated from agricultural soils and identified using Biolog microtiter assay. All Gram-ositive degrading bacterial strains belong to genus Bacillus and many Gram-negative bacteria were rare soil species. Among them fast growing strains on phospamidon-containing minimal medium were selected and their biodegrading capability were measured. YD-17 which was identified as Capnocytophaga gingivalis showed the highest biodegradation rate. It could increase the removal of phosphamidon up to 52%. During the biodegradation continuous increase of amount of cell protein was observed, which, indicated that phosphamidon was utilized as a carbon source for phosphamidon-degrading bacteria.

통신망의 효율적 통합관리를 위한 예비비트 활용방안

강양구,김명조,이성로,김경호 木浦大學校 應用科學硏究院 2001 應用科學硏究誌 Vol.1 No.-

In this paper, a new method for improve the performance of was presented. This method based on the use of an idle overhead in the TCP/IP protocol that used in syhchronous network structure of TMN system. Two type of modeling methodes were executed to show the performance improvements. First, it materializes the functions of the network condition scan and the remote control login. Second, this paper shows the control of a subsystem by the convert process of protocol which uses a signal conversion.

방송산업과 저작권

강태영,최양수,김영석 연세대학교 언론연구소 1992 언론 사회 문화 Vol.- No.2

상악 협측 치은에 발생한 모세혈관종 치험례 : A CASE REPORT

강근영,양규호,최남기,김선미 大韓小兒齒科學會 2004 大韓小兒齒科學會誌 Vol.31 No.4

혈관종은 혈관의 증식에 의해 발생하는 양성 종양으로 유아와 어린이에서 호발한다. 대부분 진성종양으로 보다는 과오종(hamartoma)으로 보며 구강내 호발부위는 입술, 혀, 협점막 구개부 등이다. 임상소견은 편평하거나 융기된 적청색 병소로 보통 단발성이다. 조직학적 소견을 통해 모세혈관성(capillary), 해면상(cavernous), 혼합성(mixed), 경화성(sclerosing)등으로 분류되며 확진된다. 본 증례는 6세 남자 환아가 치은에 뭐가 났다는 것을 주소로 전남대학교 병원 소아치과에 내원하였다. 임상검사 결과 #62, 63 부위의 부착치은에 딸기모양 종물(mass)이 관찰되었다. 조직검사 시행 결과 모세혈관종으로 확진되었으며 외과적 절제로 양호한 결과를 얻었기에 보고하는 바이다. 혈관종은 재발이 드물며 악성전환이 없지만 출혈 경향이 높으므로 임상적 시술시 주의가 요구된다. The hemangioma, a benign proliferation of blood vessel, is the most common tumor of infancy and childhood. In many instances, the lesion probably represents a hamartoma or malformation rather than a true neoplasm. In the oral cavity, common sites are lips, followed by tongue, buccal mucosa and palate. Clinical characteristics appear as a flat or raised reddish-blue lesion and are generally solitary. They are classified on the basis of their histological appearance into capillary, mixed, cavernous or a sclerosing variety. A 6-year-old male of this case was referred to the Department of Pediatric Dentistry, Chonnam National University with a chief complaint of swelling lesion on gingiva. The strawberry appearance mass was detected by clinical examination on attached gingiva at the upper left primary lateral incisor and canine. Surgical excision and biopsy were carried out for histological examination and the lesion was diagnosed with a capillary hemangioma. The risk of recurrence after this therapy is rare, and there is no malignant transformation. Despite their benign origins and behaviour, hemangiomas in the region of oral cavity are always clinically important to the dental profession because of bleeding tendency.

방재관점에서 본 서울시 주택재개발 방향

姜良錫 弘益大學校 科學技術硏究所 2007 科學技術硏究論文集 Vol.18 No.-

Nonetheless there has been almost no seisrruc disaster, there are average 40 earthquakes per annum in the Korean Peninsula. Therefore the densely built-up area with masonry buildings, which is extremely fragile to an earthquake, should be redeveloped to improve the urban conditions in terms of disaster prevention. However, it is not concerned about the seismic fragility of buildinzs, but about the year of built as well as the width of roads according to the 'Act on the Maintenance and Improvement of Urban Areas and Dwelling Conditions for Residents'. This research examined the possible problems under the severe seismic situation in the sample areas which are densely built-up with masonry buildings in Seoul. Based upon the result, the direction of housing renewal plan from a viewpoint of disaster prevention are proposed.