Theoretic Analysis on the Manufacture of Blended Yarn by One Spinneret

Wang, Ni,Zhang, Jianchun,Lai, Kan,Sun, Run-Jun The Korean Fiber Society 2007 Fibers and polymers Vol.8 No.3

The possibility of producing blended yam by using one spinneret is analyzed theoretically, especially the formation of differences in linear density and shrinkage between the monofils. Under the same spinning pressure, the die-spinning nozzles with different diameters are used to produce the differences in the flow of the melt. According to the hagen-poseuille equation for streamline flow in cylinder pipe, the volumetric flow rate and the mean melt speed of die-spinning nozzles increase with the increase of diameters. Under the conditions that the winding speeds are the same, so that the effective draw ratios decrease with the increase of the die-spinning nozzles diameters. Then, the filament formed through the die-spinning nozzle with large diameter is coarse and have a low birefringence. On the contrary, the filament formed through the die-spinning nozzle with small diameter is fine and have a high birefringence. The differences between the filament in structure and property could be acquired by one spinneret.

Nicotinamide Mononucleotide Adenylyl Transferase 2 Inhibition Aggravates Neurological Damage after Traumatic Brain Injury in a Rat Model

Xiaoyu Gu,Haibo Ni,XuGang Kan,Chen Chen,Zhiping Zhou,Zheng Ding,Di Li,Bofei Liu 대한신경외과학회 2023 Journal of Korean neurosurgical society Vol.66 No.4

Objective : Nicotinamide mononucleotide adenylyl transferase 2 (NMNAT2) is a crucial factor for the survival of neuron. The role of NMNAT2 in damage following traumatic brain injury (TBI) remains unknown. This study was designed to investigate the role of NMNAT2 in TBI-induced neuronal degeneration and neurological deficits in rats. Methods : The TBI model was established in Sprague-Dawley rats by a weight-dropping method. Real-time polymerase chain reaction, western blot, immunofluorescence, Fluoro-Jade C staining, and neurological score analyses were carried out. Results : NMNAT2 mRNA and protein levels were increased in the injured-side cortex at 6 hours and peaked 12 hours after TBI. Knocking down NMNAT2 with an injection of small interfering RNA in lateral ventricle significantly exacerbated neuronal degeneration and neurological deficits after TBI, which were accompanied by increased expression of BCL-2-associated X protein (Bax). Conclusion : NMNAT2 expression is increased and NMNAT2 exhibits neuroprotective activity in the early stages after TBI, and Bax signaling pathway may be involved in the process. Thus, NMNAT2 is likely to be an important target to prevent secondary damage following TBI.

Preparation and characterization of hexaferrites Ba0.40Sr0.60-xNdxFe12.00-xZnxO19 by the solid-state reaction route

Yujie Yang,Xiansong Liu,Shuangjiu Feng,Xucai Kan,Qingrong Lv,Feng Hu,Jiangli Ni,Chaocheng Liu,Wei Wang 한양대학교 세라믹연구소 2020 Journal of Ceramic Processing Research Vol.21 No.4

The microstructural, spectral, magnetic and electric properties of hexaferrites Ba0.40Sr0.60-xNdxFe12.00-xZnxO19 (0.00 ≤ x ≤ 0.30)synthesized by the solid-state reaction route have been studied. XRD results confirmed that the hexaferrites with Nd-Zncontent (x) of 0.00 ≤ x ≤ 0.24 were single M-type phase, and the hexaferrite with x = 0.30 exhibited the M-type phase andimpurity phase. The remanence (Br) increased with x from 0.00 to 0.06, and then decreased when x ≥ 0.06. The intrinsiccoercivity (Hcj) and magnetic induction coercivity (Hcb) decreased with x from 0.00 to 0.30. Br indicated a linear decreasingbehavior with increasing temperature from 20 oC to 140 oC. Hcj raised linearly with increasing temperature from 20 oC to 140oC. The value of aBr basically remained constant with Nd-Zn content (x). The value of aHcj increased with x from 0.00 to 0.12,and began to decrease when x ≥ 0.12. The electrical resitivity (ρ) presented a decreasing trend with x from 0.00 to 0.30.

Elevated Expression of RIOK1 Is Correlated with Breast Cancer Hormone Receptor Status and Promotes Cancer Progression

Zhiqi Huang,Xingyu Li,Tian Xie,Changjiang Gu,Kan Ni,Qingqing Yin,Xiaolei Cao,Chunhui Zhang 대한암학회 2020 Cancer Research and Treatment Vol.52 No.4

Purpose RIOK1 has been proved to play an important role in cancer cell proliferation and migration in various types of cancers—such as colorectal and gastric cancers. However, the expression of RIOK1 in breast cancer (BC) and the relationship between RIOK1 expression and the development of BC are not well characterized. In this study, we assessed the expression of RIOK1 in BC and evaluated the mechanisms underlying its biological function in this disease context. Materials and Methods We used immunohistochemistry, western blot and quantitative real-time polymerase chain reaction to evaluate the expression of RIOK1 in BC patients. Then, knockdown or overexpression of RIOK1 were used to evaluate the effect on BC cells in vitro and in vivo. Finally, we predicted miR-204-5p could be a potential regulator of RIOK1. Results We found that the expression levels of RIOK1 were significantly higher in hormone receptor (HR)–negative BC patients and was associated with tumor grades (p=0.010) and p53 expression (p=0.008) and survival duration (p=0.011). Kaplan-Meier analysis suggested a tendency for the poor prognosis. In vitro, knockdown of RIOK1 could inhibit proliferation, invasion, and induced apoptosis in HR-negative BC cells and inhibited tumorigenesis in vivo, while overexpression of RIOK1 promoted HR-positive tumor progression. MiR-204-5p could regulate RIOK1 expression and be involved in BC progression. Conclusion These findings indicate that RIOK1 expression could be a biomarker of HR-negative BC, and it may serve as an effective prognostic indicator and promote BC progression.

Vibrio parahaemolyticus cqsA controls production of quorum sensing signal molecule 3-hydroxyundecan-4-one and regulates colony morphology

Kui Wu,Yangyun Zheng,Qingping Wu,Haiying Chen,Songzhe Fu,Biao Kan,Yongyan Long,Xiansheng Ni,Junling Tu 한국미생물학회 2019 The journal of microbiology Vol.57 No.12

In order to adapt to different environments, Vibrio parahaemolyticus employed a complicated quorum sensing system to orchestrate gene expression and diverse colony morphology patterns. In this study, the function of the putative quorum sensing signal synthase gene cqsA (VPA0711 in V. parahaemolyticus strain RIMD2210633 genome) was investigated. The cloning and expression of V. parahaemolyticus cqsA in Escherichia coli system induced the production of a new quorum sensing signal that was found in its culture supernatant. The signal was purified by high performance liquid chromatography methods and determined to be 3-hydroxyundecan- 4-one by indirect and direct mass spectra assays. The deletion of cqsA in RIMD2210633 changed V. parahaemolyticus colony morphology from the classical ‘fried-egg’ shape (thick and opaque in the center, while thin and translucent in the edge) of the wild-type colony to a ‘pancake’ shape (no significant difference between the centre and the edge) of the cqsAdeleted colony. This morphological change could be restored by complementary experiment with cqsA gene or the signal extract. In addition, the expression of opaR, a well-known quorum sensing regulatory gene, could be up-regulated by cqsA deletion. Our results suggested that V. parahaemolyticus used cqsA to produce 3-hydroxyundecan-4-one signal and thereby regulated colony morphology and other quorum sensing-associated behaviors.

Comprehensive profiles and diagnostic value of menopausal-specific gut microbiota in premenopausal breast cancer

Hou Ming-Feng,Ou-Yang Fu,Li Chung-Liang,Chen Fang-Ming,Chuang Chieh-Han,Kan Jung-Yu,Wu Cheng-Che,Shih Shen-Liang,Shiau Jun-Ping,Kao Li-Chun,Kao Chieh-Ni,Lee Yi-Chen,Moi Sin-Hua,Yeh Yao-Tsung,Cheng Chi 생화학분자생물학회 2021 Experimental and molecular medicine Vol.53 No.-

In Western countries, breast cancer tends to occur in older postmenopausal women. However, in Asian countries, the proportion of younger premenopausal breast cancer patients is increasing. Increasing evidence suggests that the gut microbiota plays a critical role in breast cancer. However, studies on the gut microbiota in the context of breast cancer have mainly focused on postmenopausal breast cancer. Little is known about the gut microbiota in the context of premenopausal breast cancer. This study aimed to comprehensively explore the gut microbial profiles, diagnostic value, and functional pathways in premenopausal breast cancer patients. Here, we analyzed 267 breast cancer patients with different menopausal statuses and age-matched female controls. The α-diversity was significantly reduced in premenopausal breast cancer patients, and the β-diversity differed significantly between breast cancer patients and controls. By performing multiple analyses and classification, 14 microbial markers were identified in the different menopausal statuses of breast cancer. Bacteroides fragilis was specifically found in young women of premenopausal statuses and Klebsiella pneumoniae in older women of postmenopausal statuses. In addition, menopausal-specific microbial markers could exhibit excellent discriminatory ability in distinguishing breast cancer patients from controls. Finally, the functional pathways differed between breast cancer patients and controls. Our findings provide the first evidence that the gut microbiota in premenopausal breast cancer patients differs from that in postmenopausal breast cancer patients and shed light on menopausal-specific microbial markers for diagnosis and investigation, ultimately providing a noninvasive approach for breast cancer detection and a novel strategy for preventing premenopausal breast cancer.