Comparison of extraction methods for determining tocopherols in soybeans

Lim, Ho,Woo, Sunhee,Kim, Hong Sig,Jong, Seung-Keun,Lee, Junsoo WILEY-VCH Verlag 2007 European journal of lipid science and technology Vol.109 No.11

<P>We evaluated the usefulness of saponification, direct solvent extraction, and Soxhlet extraction as extraction methods to determine the amounts of tocopherols in soybeans. Soxhlet extraction yielded the highest analytical values for each tocopherol homolog and was the best method for quantifying tocopherols in soybeans. Coupling of simple Soxhlet extraction with HPLC provided a highly reproducible procedure to quantify tocopherols in soybeans. The percent mean recovery ± standard deviation (n = 5) was 103.2 ± 1.21, 109.8 ± 4.18, 93.8 ± 1.12, and 106.9 ± 1.54% for α-, β-, γ-, and δ-tocopherol, respectively. The linearity test for quantification was carried out over the ranges of 0.4–10.0, 0.2–4.0, 2.0–16.0, and 0.4–10.0 µg/mL for α-, β-, γ-, and δ-tocopherol, respectively. Regression analysis showed an excellent linear relationship (R<SUP>2</SUP> >0.998), and the results of the validation parameters were generally reliable and satisfactory.</P>

Butein inhibits adipocyte differentiation by modulating the AMPK pathway in 3T3-L1 cells

Lim, Yongrae,Sung, Jeehye,Yang, Jinwoo,Jeong, Heon Sang,Lee, Junsoo Wiley (Blackwell Publishing) 2018 Journal of Food Biochemistry Vol.42 No.1

Curvilinear-Coordinate-Based Object and Situation Assessment for Highly Automated Vehicles

Junsoo Kim,Kichun Jo,Wontaek Lim,Minchul Lee,Myoungho Sunwoo IEEE 2015 IEEE transactions on intelligent transportation sy Vol.16 No.3

<P>This paper presents a novel curvilinear-coordinate-based approach to improve object and situation assessment performance for highly automated vehicles under various curved road conditions. The approach integrates object information from radars and lane information from a camera with three steps: track-to-track fusion, curvilinear coordinate conversion, and lane assessment. The track-to-track fusion is achieved through a nearest neighbor filter that updates the target state estimation and covariance with the nearest neighbor measurement, and a cross-covariance method that merges the duplicate tracks using error covariance. In order to determine in which lane the fused tracks are located accurately and reliably, the curvilinear coordinate conversion process is performed. The curvilinear coordinates are generated in the form of a cubic Hermite spline lane model from the lane information of the camera. Based on the converted track information and the lane model in the curvilinear coordinates, the probability distribution of the threat levels in each lane is determined though a probabilistic lane association and threat assessment. The developed algorithm is verified and evaluated through experiments using a real-time embedded system. The results show that the proposed curvilinear-coordinate-based approach provides excellent performance of object and situation assessment, in respect of accuracy and computational efficiency, in real-time operation.</P>

Physical and Chemical Constraints on Transformation and Mass-increase of Fine Aerosols in Northeast Asia

Saehee Lim,Meehye Lee,PaoloLaj,Sang-Woo Kim,Kang-Ho Ahn,Junsoo Gil,Xiaona Shanga,Marco Zanatta,Kyeong-Sik Kang 한국대기환경학회 2021 한국대기환경학회 학술대회논문집 Vol.2021 No.10

Over the past few decades, northeast Asia has suffered from the extreme levels of PM2.5. Despite extensive efforts and the scientific advances in understanding PM2.5 pollution, the fundamental mechanisms responsible for the occurrence of high PM2.5 concentrations have not been comprehensively understood. In this study, we investigated the physical and chemical drivers for the formation and transformation of atmospheric particles using a four-year dataset of nanoparticle number size distributions, PM2.5 chemical composition, gaseous precursors, and meteorological variables in nor theast Asia outflows. The empirical orthogonal function (EOF) analyses of size-separated particle numbers extracted two modes representing a burst of nanoparticles (EOF1) and an increase in PM2.5 mass (EOF2) associated with persistent anticyclone and synoptic-scale stagnation, respectively. The vertical structure of the particles demonstrated that the synoptic conditions also affected the daily evolution of boundary layer, promoting either the formation of nanoparticles through deep mixing or conversion into accumulation-mode particles in shallow mixed layers. In the haze-development episode equivalent to EOF2 during the KORUS-AQ (KORea-US Air Quality) campaign, the PM2.5 mass reached 63 mg m<SUP>-3</SUP> with the highest contribution from inorganic constituents, which was accompanied by a thick coating of refractory black carbon (rBC) that linearly increased with condensation-mode particles. This observational evidence suggests that the thick coating of rBC resulted from an active conversion of condensable gases into particle-phase on the BC surface, thereby increasing the mass of the accumulation-mode aerosol. Consequently, this result complies with the strategy to reduce black carbon as a way to effectively mitigate haze pollution as well as climate change in northeast Asia.

Comparing Argus sling and artificial urinary sphincter in patients with moderate post-prostatectomy incontinence

Bumjin Lim,Aram Kim,Miho Song,Ji-Youn Chun,Junsoo Park,Myung-Soo Choo 한국운동재활학회 2014 JER Vol.10 No.5

승용차 운전자에 대한 사용성 평가 구조 모형 개발

박준수(Junsoo Park),박성준(Sungjoon Park),임영재(Youngjae Lim),정의승(Eui S. Jung) 대한인간공학회 2010 大韓人間工學會誌 Vol.29 No.6

This study aims to systematically develop a usability evaluation model using the Structural Equation Model (SEM) from experiment of usability on using vehicle. Vehicle developers have been adding many functions for enhance the user satisfaction. But it will be made the trade-off problem of usability and design elements of vehicle interior from attempt to make best usability satisfaction in a restricted space. To solve the trade-off problem, we set a new solution criterion from usability evaluation model. The usability experiment is based on major activity pool from derived user's acts pattern in vehicle for make more accurate usability evaluation model. And this model was built with twenty-nine measurement variables for the evaluation of usability of vehicle user. As a result, the proposed SEM model showed statistical significance as well as a high level of R Square (0.7144). This model shows the relationships of detailed usability and design elements. According to the result, this study introduces the criterion to secure the best satisfaction of usability and design elements.

Mesenchymal Stem Cell Therapy Alleviates Interstitial Cystitis by Activating Wnt Signaling Pathway

Song, Miho,Lim, Jisun,Yu, Hwan Yeul,Park, Junsoo,Chun, Ji-Youn,Jeong, Jaeho,Heo, Jinbeom,Kang, Hyunsook,Kim, YongHwan,Cho, Yong Mee,Kim, Seong Who,Oh, Wonil,Choi, Soo Jin,Jang, Sung-Wuk,Park, Sanghyeo Mary Ann Liebert 2015 STEM CELLS AND DEVELOPMENT Vol.24 No.14

Influence of Long-term Supplementation with Korean Red Ginseng on in vivo Antioxidant Capacities in Rats

Heung-Bin Lim,Dong-Wook Lee,Junsoo Lee 한국식품과학회 2009 Food Science and Biotechnology Vol.18 No.1

Effects of ginseng on in vivo antioxidant capacities with age were studied in rats. All rats were reared in the conventional system. Ginseng-treated rats were supplied with ginseng water extracts (25 ㎎/㎏/day) continuously from 6 weeks of age to spontaneous death. None of the rats showed any discernible adverse effects of treatment with ginsengcontaining water. There was no significant difference in body weight (BW) gains with age between treated and control groups. However, ginseng extracts did cause a decrease in the level of serum low density lipoprotein (LDL)-cholesterol, glucose, and thiobarbituric acid reactive substances (TBARS) in the treated rats. The activities of superoxide dismutase (SOD), catalase, and glutathione peroxidase in liver cytosol decreased with age in the control group. However, these enzyme activities were well maintained in the ginseng-treated rats and, especially, catalase and glutathione peroxidase activities were consistently higher than in control rats. The levels of total sulfhydryl group (T-SH) and glutathione reductase (GR) were unchanged, and glutathione-S-transferase (GST) activity gradually decreased with age in both groups. There were no differences in T-SH, GR, or GST between the control and treatment groups. These results indicate that long-term administration of ginseng retards agerelated deterioration in some biochemical parameters such as cholesterol, glucose, and lactate dehydrogenase in serum and it has an enhancing effect on antioxidant capacity in the liver.

건조방법에 따른 고춧가루의 품질특성

임용래(Yongre Lim),경예나(Yena Kyung),정헌상(Heon Sang Jeong),김혜영(Hae Yong Kim),황인국(In Guk Hwang),유선미(Seon Mi Yoo),이준수(Junsoo Lee) 한국식품영양과학회 2012 한국식품영양과학회지 Vol.41 No.9

본 연구에서는 건조방법에 따른 고춧가루의 품질 변화를 비교하기 위하여 한 가지 품종의 고추를 PE House 건조,열풍건조, 원적외선 방법을 이용하여 건조한 다음, 건조조건별로 고춧가루의 수분함량 변화, pH, ASTA value 및 capsaicinoids와 vitamin C의 함량을 조사하였다. 건조 후 고춧 가루의 수분함량은 12.05∼14.01% 함유한 것으로 나타났으며, pH는 건조 전 생고추인 4.93보다 다소 높은 5.00∼5.54를 나타내었다. 고춧가루의 매운맛을 나타내는 총 capsaicinoids의 함량은 PE House 건조에서 224.40 mg/100 g으로 가장 높게 나타났으며, 열풍 70oC 건조에서 191.87 mg/100g으로 가장 낮게 나타났다. 하지만 건조방법에 따른 capsaicinoids 함량이 유의적 차이를 보이지 않았기 때문에, 매운맛 함량의 변화는 큰 차이를 나타내지 않았다. 고춧가루의 대표적 항산화제인 vitamin C 함량은 다른 건조방법 및 조건에 비해 PE House, 열풍 60oC과 원적외선 60oC에서 596.60∼648.31 mg/100 g으로 높은 수준의 함량을 나타내었다. 소비자가 고춧가루를 선택하는데 중요한 요소로 작용하는 ASTA value는 126.68∼156.77로 건조방법 및 조건에 따른 유의적 차이를 나타내지 않았다. 따라서 고추의 건조방법 및 조건을 낮은 온도 조건에서의 열풍, 원적외선 건조방법으로 결정한다면 고춧가루의 vitamin C의 손실을 최소화하고 PE House 건조에 비해 신속하며 오염을 예방하는 고품질의 고춧가루 제품을 생산할 수 있을 것으로 생각된다. Red pepper (Capsicum annuum L.) powder is widely used as a spice and flavor ingredient in the food industry. It is well known that during the drying process red pepper undergoes physico-chemical and nutritional changes. The objective of this study was to investigate the quality of red pepper powder according to drying method. Red pepper was dried by far-infrared drying, hot air drying, and polyethylene (PE) house drying. Average moisture content of dried red pepper powder from the three different drying methods was 12.5±0.3%. The pH level slightly increased from 4.93 before drying to 5.00∼5.54 after drying. Contents of capsaicinoids were highest (224.40 mg/100 g) in the PE house drying method and lowest (191.87 mg/100 g) in the hot air drying (70oC) method. However, capsaicinoid contents were not significantly different among the various drying methods. Vitamin C content decreased as temperature increased. Drying conditions, particularly temperature, lead to loss of vitamin C in red pepper, resulting in quality degradation. Taken together, these results demonstrate that the content of vitamin C, one of the major factors affecting pepper powder quality, was affected by drying temperature.

3T3-L1 지방전구세포에서 홍국균 균사체-고체발효 원두커피 추출물의 지방축적 억제효과

임용래(Yongrae Lim),신지영(Ji-Young Shin),김훈(Hoon Kim),백길훈(Gil-Hun Baek),유광원(Kwang-Won Yu),정헌상(Heon-Sang Jeong),이준수(Junsoo Lee) 한국식품영양과학회 2014 한국식품영양과학회지 Vol.43 No.4

본 연구에서는 두 품종의 원두커피와 이를 Monascus ruber 홍국균으로 발효시킨 원두커피 열수추출물의 지방축적 억제활성을 확인하고자 하였다. 세포 내 triglyceride 생성 저해효과 및 주요 전사인자인 PPARγ, C/EBPα와 FAS 및 aP2의 발현을 측정하기 위해 3T3-L1 지방전구세포에서 성숙지방세포로의 분화 유도와 함께 베트남 로부스타(VR), 홍국균으로 고체발효 한 베트남 로부스타(MR-VR), 발아현미(10, 20, 30%)를 첨가하여 홍국균으로 고체발효 한 베트남로부스타(MR-VR10, MR-VR20, MR-VR30), 에티오피아모카 시다모 G2(ES), 홍국균으로 고체발효 한 에티오피아모카 시다모 G2(MR-ES), 발아현미(10, 20, 30%)를 첨가하여 홍국균으로 고체발효 한 에티오피아 모카 시다모 G2(MR-ES10, MR-ES20, MR-ES30) 원두커피의 열수추출물을 1,000 μg/mL 농도로 처리하였다. 연구 결과 대조군과 비교하여 커피추출물을 처리한 모든 실험군에서 유의적으로 지방구 생성이 감소하였다. 베트남 로부스타 품종이 에티오피아 모카 시다모 G2 품종보다 지방구 생성 및 지방분화전사인자들인 PPARγ, C/EBPα, FAS 및 aP2의 발현을 효과적으로 억제하였으며, 에티오피아 모카 시다모 G2 품종의 경우 원두커피 열수추출물을 처리한 실험군보다 홍국균으로 고체발효 한 원두커피의 열수추출물을 처리한 실험군에서 더 높은 지방분화 억제능을 나타냈다. 또한 전사인자들의 발현 정도는 지방분화 억제능의 결과와 유사하였다. 따라서 Monascus ruber 홍국균의 고체배양을 이용한 에피오티아 모카 시다모 G2 발효원두커피는 효과적인 항비만 기능성 식품으로서의 활용가치가 기대되며 로부스타 품종의 경우 다른 품종들보다 저렴한 원가를 감안할 때 베트남 로부스타 발효원두커피는 경제적인 기능성 커피음료 및 기능성 소재로서 산업적인 응용에 좋은 활용가치가 될 것으로 사료된다. Obesity is the leading metabolic disease in industrialized countries and is closely associated with coronary heart disease, hypertension, diabetes, and cancer. The objective of this study was to evaluate the anti-adipogenic effects of two roasted coffee beans, Vietnam robusta (VR) and Ethiopia Mocha Sidamo G2 (ES), as well as fermented coffee beans with Monascus ruber (MR) mycelium on differentiation of 3T3-L1 preadipocytes. Treatments with 1,000 μg/mL of hot water extract from coffee beans significantly reduced intracellular lipid accumulation. In addition, VR more effectively inhibited transcription factors such as PPARγ, C/EBPα, FAS, and aP2 compared to ES. Further, ES fermented with MR showed more effective anti-adipogenic activity than non-fermented ES. These results suggest that VR and ES inhibit adipocyte differentiation which may contribute to their anti-adipogenic properties.