S-1 plus irinotecan and oxaliplatin for the first-line treatment of patients with metastatic colorectal cancer: a prospective phase II study and pharmacogenetic analysis

Kim, S Y,S Hong, Y,K Shim, E,Kong, S-Y,Shin, A,Baek, J Y,Jung, K H Nature Publishing Group 2013 The British journal of cancer Vol.109 No.6

<P><B>Background:</B></P><P>S-1 is an oral fluoropyrimidine that mimics infusional 5-fluorouracil. The aim of this phase II trial was to explore the clinical efficacy of the triplet regimen TIROX, which consists of S-1, irinotecan and oxaliplatin.</P><P><B>Methods:</B></P><P>Forty-two chemo-naive patients with metastatic colorectal cancer (mCRC) were planned to be enrolled and be treated with irinotecan 150 mg m<SUP>−2</SUP> followed by oxaliplatin 85 mg m<SUP>−2</SUP> on day 1 and S-1 80 mg m<SUP>−2</SUP> per day from day 1 to 14 every 3 weeks. Polymorphisms in the <I>UGT1A1</I>, <I>UGT1A6</I>, <I>UGT1A7</I> and <I>CYP2A6</I> genes were analysed.</P><P><B>Results:</B></P><P>Between July 2007 and February 2008, 43 patients were enrolled. An objective response was noted in 29 patients (67.4%, 95% confidence interval: 53.4–81.4), of which 2 achieved durable complete responses. The median progression-free survival was 10.0 months and the median overall survival was 19.2 months. Significant grade 3 or 4 adverse events were neutropenia (45.2%), febrile neutropenia (9.5%), diarrhoea (7.1%) and vomiting (9.5%). Increased gastrointestinal toxicities were associated with the presence of <I>UGT1A6*2</I> or <I>UGT1A7*3</I> and an improved tumour response was noted in those without variant alleles of <I>CYP2A6</I> or <I>UGT1A1*60</I>.</P><P><B>Conclusion:</B></P><P>The combination of S-1, irinotecan and oxaliplatin showed favourable efficacy and tolerability in untreated patients with mCRC.</P>

Apple ethanol extract promotes proliferation of human adult stem cells, which involves the regenerative potential of stem cells

Lee, J.,Shin, M.S.,Kim, M.O.,Jang, S.,Oh, S.W.,Kang, M.,Jung, K.,Park, Y.S.,Lee, J. Pergamon Press 2016 Nutrition research Vol.36 No.9

<P>Tissue regeneration using adult stem cells (ASCs) has significant potential as a novel treatment for many degenerative diseases. Previous studies have established that age negatively affects the proliferation status and differentiation potential of ASCs, suggesting a possible limitation in their potential therapeutic use. Therefore, we hypothesized that apple extract might exert beneficial effects on ASCs. The specific objectives were to investigate the proliferative effect of apple ethanol extract on human adipose tissue-derived mesenchymal stem cells (ADSCs) and human cord blood-derived mesenchymal stem cells (CB-MSCs), and identify the possible molecular mechanisms. Apple extract promoted proliferation of ADSCs and CB-MSCs as determined by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide and Click-iT 5-ethynyl-2'-deoxyuridine flow cytometry assays. In addition, phosphorylation of p44/42 MAPK (ERK), mammalian target of rapamycin (mTOR), p70 S6 kinase (p70S6K), S6 ribosomal protein (S6RP), eukaryotic initiation factor (eIF) 4B and eIF4E was induced stepwise in ADSCs. Furthermore, apple extract significantly induced the production of vascular endothelial growth factor and interleukin-6 in both ADSCs and CB-MSCs. Similarly, apple extract-induced phosphorylation of the mTOR/p70S6K/S6RP/eIF4B/eIF4E pathway was blocked by pretreatment with PD98059, a specific ERK inhibitor. These results indicate that apple extract-induced proliferation of ADSCs under serum-free conditions is mediated by ERK-dependent cytokine production. Moreover, the beneficial effect of apple extract on proliferation of ASCs may overcome the limitation in therapeutic use of stem cells in tissue regeneration and maintenance of stem cell homeostasis. (C) 2016 Elsevier Inc. All rights reserved.</P>

Antibiotic susceptibility and resistance of Streptococcus iniae and Streptococcus parauberis isolated from olive flounder (Paralichthys olivaceus)

Park, Y.K.,Nho, S.W.,Shin, G.W.,Park, S.B.,Jang, H.B.,Cha, I.S.,Ha, M.A.,Kim, Y.R.,Dalvi, R.S.,Kang, B.J.,Jung, T.S. Elsevier Scientific Pub. Co 2009 Veterinary microbiology Vol.136 No.1

The rates of antibiotic susceptibility and resistance were investigated in Streptococcus iniae and Streptococcus parauberis isolates obtained from diseased olive flounders (Paralichthys olivaceus) collected from fish farms in Jeju Island, Korea. Isolates of S. iniae (n=65) were susceptible to cefotaxime, erythromycin, ofloxacin, penicillin, tetracycline and vancomycin, as demonstrated by the minimum inhibitory concentration (MIC) test. Isolates of S. parauberis (n=86) were highly resistant to erythromycin (58% of the 86 isolates tested) and tetracycline (63% of the 86 isolates tested). Fifty-four isolates of tetracycline-resistant S. parauberis contained the tet(M/O/S) genes, of which 39 and 12 isolates contained the tet(M) and tet(S) genes, respectively, whereas 3 isolates contained both the tet(M) and tet(S) genes. Among the erythromycin-resistant isolates of S. parauberis (n=50) only 14 contained the erm(B) gene. These results suggest that the tet(S) and erm(B) genes of S. parauberis are involved in the acquisition of high-level resistance to erythromycin and tetracycline. Our findings reveal a high rate of antibiotic resistance among strains of S. parauberis and emphasize the need to develop an appropriate vaccine to reduce the use of antibiotics.

Genome Sequence of Corynebacterium nuruki S6-4T, Isolated from Alcohol Fermentation Starter

Shin, N.-R.,Whon, T. W.,Roh, S. W.,Kim, M.-S.,Jung, M.-J.,Lee, J.,Bae, J.-W. American Society for Microbiology 2011 Journal of Bacteriology Vol.193 No.16

Urokinase loading Dose와 Maintenance Dose를 위한 연구

홍세용,양동호,신현길,김순길,오도연,김기용,기은경,정광회,신상구 대한내과학회 1992 대한내과학회지 Vol.42 No.1

저자들은 two chain urokinase의 loading dose 및 maintenance dose를 결정하기 위하여 성인 뇌졸중 환자 6예를 대상으로 urokinase 300,000units를 주입하고(Ⅳ boluse), 60분간 혈중 urokinase의 변동을 관찰하여 two compartment model을 이용한 pharmacokinetic study를 하여 다음과 같은 결과를 얻었다. 체중의 55~65㎏ 정도이고 간 기능이 정상인 한국인 성인에서 urokinase의 Vc(central compartment volume)은 4.56L 이었고 반감기는 distribution phase에서 2.3분, elimination phase에서 33.1분이었다. Total boidy clearance(CL)은 244/min이었고 area under the concentration time curve(AUC)는 1,349units/㎖.min이었다. 이를 근거로 하였을때 시험관에서 free form plasmin을 유리하는 urokinase의 최소 농도인 10units를 maintenance dose로 시간당 146,400units를 주입하는 것이 적절한 것으로 판단되었으며 이는 외국 성서에서 추천하는 시간당 4,400units/㎏(60㎏×4,400=264,00units)에 비하여 약 절반 정도에 해당하는 양이다. 이렇게 차이가 나는 이유는 명확하지 않으나 본 연구에서 desired concentration으로 사용한 10units/㎖은 소위 therapeutic window의 lower margin에 해당되기 때문일 것으로 생각되며 therapeutic window의 upper magrin을 규명하기 위한 연구가 뒤따라야 할 것으로 사료된다. A Pharmacokinetic study was performed .for two-chain UK(M.W.53,000) in 6 Korean adults with body weight between 55~65㎏ Following intravenous administration of 300,000 units of UK, concentration declined rapidly. The half life showed two-compartment disposition with mean initial and terminal half-lives of 2.3 and 33.1 minutes. Total body clearance was about 244㎖/min and the steady state volume of distribution (Vdss) was 9.46L. In conjunction with our previous finding that free form plasmin begin to form at a urokinase concentration of 5~10units/㎖, our results suggests that the loading dose and maintenance dose of urokinase during systemic intravenous administration should be about 45, 610unit and 146,400unit/hr in a Korean adult.

상용 S/W를 이용한 계류선 설계

정동호(D.H. JUNG),신승호(S.H. SHIN),남보우(B.W. Nam),김현주(H.J. KIM),이호생(H.S. LEE),문덕수(D.S. MOON) 한국해양환경·에너지학회 2012 한국해양환경공학회 학술대회논문집 Vol.2012 No.5

A mooring line for keeping the position of a floating wave energy conversion is designed with using a commercial software, Ores flex. Static analysis for one line is performed to decide the properties and pretension of a mooring line. With considering the ocean environmental condition and importance of a floating structure, the layout of multi-line is determined. The result of free decay of the designed mooring system estimates that the designed mooring system meets the resonance on the extreme ocean environment si condition. The initial design for mooring line is inevitably modified with adjustment of pretension. Adjustment of pretension with the change of length of mooring line results in the decrease of the ultimate tension on mooring line. The extreme value in tension of mooring line shows to be very sensitive to pretension of mooring line.

Identification of stathmin 1 expression induced by Epstein–Barr virus in human B lymphocytes

Baik, S. Y.,Yun, H. S.,Lee, H. J.,Lee, M. H.,Jung, S. E.,Kim, J. W.,Jeon, J. P.,Shin, Y. K.,Rhee, H. S.,Kimm, K. C.,Han, B. G. Blackwell Publishing Ltd 2007 Cell proliferation Vol.40 No.2

<P>Abstract. </P><P><I>Introduction</I>: The Epstein–Barr virus transforms resting B cells into proliferating lymphoblastoid cells, the origin of cell lines. <I>Method and results</I>: Our cDNA microarray analyses led to the identification of 232 up-regulated and 112 down-regulated genes with more than a 3-fold difference in lymphoblastoid cell lines compared to resting B cells. The functional classification of these genes exhibited the distinct expression signature for cell proliferation, cell cycle and an immune response. Among them, we verified the differential expression of several oncogenes such as <I>stathmin 1</I> (<I>STMN1</I>), <I>RAB27A</I>, <I>RAB9A</I>, <I>BACH1</I> and <I>BACH2</I> using quantitative real-time reverse transcriptase-polymerase chain reactions or Western blot analysis. Expression of <I>STMN1</I> (which is involved in regulation of the microtubule filament system, cell growth and S-phase of cell cycle) was increased in lymphoblastoid cell line as well as in 7-day post-Epstein–Barr virus infection B cells, compared to resting B cells. <I>Conclusion</I>: Thus, this study suggests that Epstein–Barr virus infection induces <I>STMN1</I> expression, which play a role in cell cycle progression and proliferation in the human B lymphocyte.</P>

Enhanced efficiency and stability of polymer solar cells using solution-processed nickel oxide as hole transport material

Parthiban, S.,Kim, S.,Tamilavan, V.,Lee, J.,Shin, I.,Yuvaraj, D.,Jung, Y.K.,Hyun, M.H.,Jeong, J.H.,Park, S.H. ELSEVIER 2017 Current Applied Physics Vol.17 No.10

<P>Solution-processed nickel oxide (s-NiOx) was synthesized for use as hole-transport layers (HTLs) in the fabrication of polymer solar cell (PSC) devices. The s-NiOx thin-films were deposited using spin-coating and post-annealed at 300 degrees C, 400 degrees C, or 500 degrees C. With increased annealing temperature, the nickel acetate precursor decomposes more fully and forms s-NiOx films that show larger crystalline grain sizes with lower root mean square surface roughness. Bulk heterojunction solar cells fabricated with the new random polymer RP(BDT-PDBT) and [6,6]-phenyl-C-70-butyric acid methyl ester (PC70BM) using s-NiOx as HTLs exhibit a 4.46% enhancement in power conversion efficiency and better stability compared to conventional PSCs using poly (3,4-ethylenedioxythiophene):poly(styrene sulfonate) as HTLs. We believe that the solution-processable and highly stable s-NiOx could be a potential alternative for functional interface materials in optoelectronic devices. (C) 2017 Elsevier B.V. All rights reserved.</P>

상용 S/W를 이용한 계류선 설계

정동호(D.H. JUNG),신승호 (S.H. SHIN),남보우(B.W. Nam),김현주(H.J. KIM),이호생(H.S. LEE),문덕수(D.S. MOON) 대한조선학회 2012 대한조선학회 학술대회자료집 Vol.2012 No.5

A mooring line for keeping the position of a floating wave energy conversion is designed with using a commercial software, Orcaflex. Static analysis for one line is performed to decide the properties and pretension of a mooring line. With considering the ocean environmental condition and importance of a floating structure, the layout of multi-line is determined. The result of free decay of the designed mooring system estimates that the designed mooring system meets the resonance on the extreme ocean environmental condition. The initial design for mooring line is inevitably modified with adjustment of pretension. Adjustment of pretension with the change of length of mooring line results in the decrease of the ultimate tension on mooring line. The extreme value in tension of mooring line shows to be very sensitive to pretension of mooring line.

고농도 Kondo 계인 Ce_0.8Ta_0.2Te_2의 전자기적 특성에 관한 연구

조은상,조정현,박상천,신용숙,정명화,권용성 성균관대학교 기초과학연구소 1997 論文集 Vol.48 No.-

The Ce_0.8Ta_0.2Te_2 single crystal was grown by using pre-reaction and mineralization method. From X-ray diffraction experiments, We can know that the Ce_0.8Ta_0.2Te_2 has tetragonal structure with being a=4.653Å and c= 9.305Å. We processed electric, magnetic and thermal experiments. In Electric resistivity, one of main peaks is sharp-shape peak at 6K, the other is broad-shape peak near 150K. The origin of 6K peak is guessed magnetic transition. This idea is confirmed by measurement of electric resistivity in various magnetic field. Specific heat measurement was done by using an adiabatic method in 1.8K-120K region. A sharp-shape peak was detected at 4.4K. We guess the origin of the sharp peak is due to a magnetic transition. The peak temperature is matched to temperature of measurement of magnetization. Magnetic susceptibility was translated on effective Bohr magneton (μ_eff) Curie-Weiss law. The value of μ_eff (=2.42_μB) give information of which Ce is composed on 3+ ion to us. (experimental value Ce μ_eff = 2.4_μB).