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Tiantian Lian,Tao Yang,Junde Sun,Suping Guo,Huaijun Yang,Caihong Dong 한국미생물학회 2014 The journal of microbiology Vol.52 No.8
Cordyceps militaris, the type species of genus Cordyceps, isone of the most popular mushrooms and a nutraceutical ineastern Asia. It is considered a model organism for thestudy of Cordyceps species because it can complete its lifecycle when cultured in vitro. In the present study, the occurrenceand sequence variation of SSU rDNA group I introns,Cmi.S943 and Cmi.S1199, among different isolatesof C. militaris were analyzed. Based on the secondary structurepredictions, the Cmi.S943 intron has been placed insubgroup IC1, and the Cmi.S1199 intron has been placedin subgroup IE. No significant similarity between Cmi.S943and Cmi.S1199 suggested different origins. Three genotypes,based on the frequency and distribution of introns, were describedto discriminate the 57 surveyed C. militaris strains. It was found that the genotype was related to the stromacharacteristics. The stromata of all of the genotype II strains,which possessed only Cmi.S943, could produce perithecium. In contrast, the stromata of all genotype III strains, whichhad both Cmi.S943 and Cmi.S1199, could not produce perithecium. Cmi.S1199 showed the lowest level of intra-specificvariation among the tested strains. Group I introns can belost during strain cross-mating. Therefore, we presumed thatduring cross-mating and recombination, intron loss could bedriven by positive Darwinian selection due to the energeticcost of transcribing long introns.